This publication discusses the protective effects of on the body under conditions of exam stress. The purpose of this study was to determine the effect of a herbal remedy «Aroura Tan No. 7» on functional state students during exam stress. The study was conducted at the Centre for Health Centre for medical prevention of Ulan-Ude. Functional state of these students was evaluated by the temporal and spectral analysis of heart rate variability (HRV). Analysis of the spectral characteristics of HRV showed that during the exam total spectral power was reduced, compared with those obtained in a state of the regular school day. In addition, examination stress causes an increase in the proportion of very slow-wave period (WLF). Psycho-emotional stress during exams show lower negative impact on the functioning of the nervous and cardiovascular systems of the students who took «Aroura Tan No. 7».

Humeroscapular periarthritis makes 80 % of all «rheumatic» diseases of humerus. Tendons of muscles, attaching in the area of shoulderjoint, are constantly in a state of greatfunctional tension, connected with shoulder abduction and rotation, which brings to early development of degenerative process in them. Taking into consideration tendency of all kinds of primary periarthritis to persistent and long course caused by very slow resorption of degeneration foci and calcificates, the main condition of the right course of treatment is its persistence and perseverance. Together with all variety of existing methods of treatment of humeroscapular periarthritis this problem is not at all solved and prevalence of humeroscapular periarthritis does not reduce. Nowadays in treatment humeroscapular periarthritis of more and more attention is paid to the methods of traditional therapy: acupuncture, pharmacopuncture, osteopathy. It is doubtless that reflexotherapy provides impact on the most part of the links of pathological process, influencing local changes in tissues and organs, normalizing nervous regulatory processes and eliminating pain. Using neuroreflex approach in diagnostics and treatment of humeroscapular periarthritis we can perform grounded and selective choice of acupuncture points, providing influence on majority of links of pathological process, which rises the efficacy of treatment, prolongs remission period, eliminates pain.

The purpose of the research was the study of the effectiveness of balloon angioplasty and stenting of renal arteries to improve the microcirculation of the kidneys in patients with renovascular hypertension. During the period from 2010 to 2012 inclusive in the vascular compartment BSMU clinic were examined and treated 32 patients with renal artery stenosis. Inclusion criteria were: presence of verified violations of regional circulation in the kidney, the presence of symptomatic renovascular hypertension. Measures the average systolic and diastolic blood pressure mast. Patients received antihypertensive treatment to endovascular treatment. Treatment with antihypertensive medication was effective only in 9.8 % of cases. Diagnostic angiography of the renal arteries was performed using angiographic complex INNOVA 3131 IQ. After analyzing the data, the question of stenting or balloon angioplasty. After a detailed survey of 32 patients underwent endovascular intervention for renal artery 6 balloon angioplasty and stenting 26. Following the procedure, the hypotensive effect was observed in all cases. Evaluation of the results showed a significant reduction in the maximum rise in systolic blood pressure by 28 %, diastolic - 21 %. After endovascular intervention, the mean systolic blood pressure decreased by 10%, the average diastolic blood pressure - 15 %. Mean arterial pressure decreased by 8 %. In a review of the effectiveness of endovascular procedures with the original length and the degree of hypertension. Thus, roentgenendovascular treatment improves microcirculation in the renal tissue, which leads to the hypotensive effect of a predominantly marked reduction of maximum systolic blood pressure.

Aim. Studies on the role of autonomic disorders, changes of serum NEFA, glycerol, adenine nucleotides and violation fraction of the fatty acids of erythrocyte membranes in the development of cardiac arrhythmias in patients with chronic prostatitis. Methods. The study included 50 patients with chronic prostatitis, mean age 35 ± 5,6 years. Vegetative state is defined by: clinical evaluation of autonomic disorders, autonomic profiles and vegetative index Kerdo. Performed Holter ECG monitoring with analysis of heart rate variability. The blood was determined NEFA, glycerol, macroergs concentration and size distribution of higher fatty acids in red blood cells. Results. The majority of patients with chronic prostatitis on the questionnaire data revealed sympathotony manifested reduced the basic parameters of heart rate variability. In these patients have the syndrome disorders utilization of fatty acids, characterized by the accumulation in the blood NEFA while reducing the level of glycerol, and decreased ATP content in red blood cells and increased AMP. The greatest changes in the level and composition of free fatty acids in erythrocyte membranes as raising saturated and polyunsaturated reduction deal with chronic prostatitis with the activation of the sympathetic autonomous nervous system level. Among patients simpatotonikov often recorded supraventricular and ventricular arrhythmias, whereas patients-eytonik arrhythmias detected less frequently, and they are only a single supraventricular extrasystoles. Thus, long-term activation of the sympathetic nervous system in patients with chronic prostatitis, manifested by reduction of basic parameters of heart rate variability leads to a syndrome disorders utilization of fatty acids in the myocardium, the energy deficit and implement the lipolytic effect of catecholamines from the imbalance of the quality of the free fatty acids, which can be a trigger mechanism in the development of cardiac arrhythmias in these patients.

To study the parameters of peripheral blood circulation of upper extremity in patients with fractures of radial bone at perosseous osteosynthesis we studied the group of patients with fractures externally fixed by apparatus with pin arrangement and the group of patients treated by apparatus of rod arrangement. Rheovasographic examination of upper extremities was performed before the surgery, the day after it, on the 7^th, 14^th and 21^st day after the surgery. In all patients during preoperational period we revealed disturbances of blood circulation in distal parts of upper extremities of angiospastic type giving evidence of sympathicotonia. Having analized the results of clinical and rheovasographic researches in established terms of observation we were able to mark gradual improvement of arterial and venous blood flow in forearm in examined groups. In the group with rod fixation positive dynamics occurred 5-7 days earlier than in the group with pin fixation. Original method was applied, consisting in rod fixation of apparatus of external fixation. Evaluation of clinical parameters marks good results in 97 % of cases. In early postoperative period improvement of rheovasographic parameters has been marked, which says about increased blood perfusion in tissues and about normalized vessel tone.

There were 81 patients with vertebral canal stenosis under observation. All patients underwent complex examination: study of clinical neurological status; review spondylography of lumbar part of the spine; MRI of lumbar part of the spine; multispiral CT with myelography of lumbar part of the spine; functional spondylography of lumbar part of the spine with load; the study of morphometry of vertebral canal, dural sack and vertebra body by designed method; the study of acute phase proteins in blood. Conducted complex research revealed that improved classification of stenosis of vertebral canal reflects direct dependence of severity of clinical-neurological manifestations from the degree of stenosis. It also has been marked that reduced concentration of α2-macroglobulin in blood manifests itself in negative regulation of cells of immune system. And an increase of classic rheumatoid factor (Ig M-RF), α1-antitripsin and α1-acid glycoprotein in blood serum leads to reduction of inflammatory and immune response. Revealed direct co-dependence of severity of disease and degree of stenosis gives a possibility to develop more precise program of conservative and surgical treatment.

In given article the results of the research of application of cytoflavin in patients with a syndrome of diabetic foot complicated by trophic ulcers of the 1-2 degree according to Wagner classification are described. The main place is taken by the interpretation of clinical and laboratory and instrumental marks, such as stimulation of reparative processes and reduction middle time of restoration of skin at the ulcer defects areas of the foot of 1-2 degree, significantly expressed reduction middle marks of glycemic profile at the stages of the research, normalization of indices of lipid peroxidation (malondialdehyde and catalase) and redress the imbalance of patients' prooxidant-antioxidant system in the blood serum, improvement clinical and instrumental marks of blood flow, such as Doppler ultrasound of the arteries of the lower limbs, average linear velocity of blood flow (Vaver), peripheral resistance index (Ri), systolic-diastolic ratio (1SD) right after the taken therapy. The dynamics of the changes of ankle-brachial index illustrating the clinical example is also given.

1n the clinic of Scientific Center of Reconstructive and Restorative Surgery SB RAMS of Irkutsk the open controlled randomized single-center study for evaluation of clinical efficiency of the surgical method was hold. The analysis of surgery was based on 34 patients with chronic posttraumatic anteromedial instability of the shoulder joint with the defect of the front edge of the blade articular process. The result of the research showed its efficiency in patients of young and working age. While evaluation of functional results by the scale of Rowe-Zarins in three months for 28,8 % and in six months for 16,4 % and by the scale of DASH for 18,5 % in three months after the surgery the better results were achieved (the level of evidence was 11 b) comparing to traditional method of surgery, that let to the patient of the main group much earlier get back to work and going in for sport.

In population based study were determine the prevalence of lumbar osteochondrose in teleuts population which are the aboriginal in Kemerovo region. It is shown, that the frequency of lumbar osteochondrose are different, depended from male, age, social status, profession and residence. The frequency of vertebral and extravertebral implications of lumbar osteochondrose is defined.

The purpose of the given work is specification adverse prognostical criteria of outcomes of treatment of acute nonspecific empiema pleurae. Work is based on the results of complex treatment of 123 patients with empiema pleurae of not traumatic character. The acute abscess and a lung gangrene (37,9 %) was a principal cause of development of empiema. It is established that the most authentic factors of failures of treatment of empiema were overdue diagnostics acute empiema pleurae; defects and errors in treatment; insufficient preoperative preparation of the patient; an insufficient skill level of the surgeon; late reference for medical aid. Preventive maintenance of the specified defects of rendering of medical aid for the patient with acute empiema pleurae will allow to improve results of treatment of patients with the given pathology.

Till these days the problem of restoration of the function of damaged flexor tendons of fingers is unsolved. A.M. Volkova consider that none of the surgical department has so many disappointments of unsatisfactory results as in surgery of wrist flexor. Functional result of treatment is defined by: 1) the level of damage; 2) means suture; 3) suture material. Active role of a hand in human activity defines high frequency of damages of this segment. More often 1-2 zone subjected to trauma, it is connected with involuntary defensive reaction at the moment of trauma. The most difficult is to restore flexor tendons at the level of bone and fibrous channels. In this work the analysis of further results of treatment of patients had surgery of tendon unit in the department of microsurgery is given. On the basis of the findings optimal and promising areas for surgery technics and results of treatment improving are defined.

The purpose of the article is to determine the factors contributing to the development of cervical intraepithelial neoplasia and cervical cancer during pregnancy, and the assessment of the impact of HIV infection on the progression of dysplasia of the cervix during pregnancy. 1146 pregnant women at various stages of gestation, including in 124 HIV-positive were examined. The control group included 146 pregnant women without HIV The survey included: examination, colposcopy, PAP test, PCR for HPV and Chlamydia, HlV diagnosis activity: ELISA, immune blotting, PCR, VIRAL LOAD, identification numbers of CD4 + and CD8 +. The presence of HPV in HIV-positive pregnant women is the leading risk factor for cervical dysplasia epithelium regardless of gestational age. Dysplasia may be suspected at colposcopy examination. The final diagnosis is stated morphologically. HIV in combination with CIN detected in the early stage of pregnancy, it is important to determine the risk of cervical cancer, while maintaining the pregnancy. You must assign the ART in detecting cervical intraepithelial neoplasia of 2-3 grade. When CIN 3 during pregnancy is appropriate elective caesarean section, as a way to prevent the development of cervical cancer after the birth, along with a reduced likelihood of intrapartum transmission of HIV.

Reflux esophagitis (R-E) is precancerous condition. The authors show urgency of the problem from the perspective of early detection of reflux esophagitis and its timely surgical treatment considering disappointing results of surgical and radiation and chemotherapy treatments of esophageal carcinoma. Basing on the data of modern literature and own researches the authors claim that late revealing of R-E and late surgery need the treatment of bronchoobstructive and pulmonary complications as well as esophageal carcinoma. Great spread of R-E in Russia and abroad proves the data of own researches. In early diagnostics of R-E the authors pay much attention to described endoscopic criteria of R-E, they are lack the reflex function of the cardia, shortening of the esophagus and other endoscopic signs of complicated forms of R-E. They can serve as guide for timely diagnostics and appointment for surgery. The given clinical observations prove the risk of bronchial complecations and esophageal carcinoma at R-E and show the efficiency of surgical treatment in the case of complications.

The aim of the work was to evaluate the effectiveness of endovenous laser ablation of great and small saphenous veins by a series of clinical cases of patients with varicose veins. Endovenous laser ablation of great (18) and small saphenous veins (8) were performed in 24 patients. This surgery was combined with phlebectomy varicose veins in 16 and foam form sclerotherapy in 10 cases. All patients werefollowed up for 2 and 6 months. Full ablation of main saphenous vein achieved in all patients in the early period of observation. Endovenous laser ablation of main saphenous veins is a good alternative to standard flebectomy it has the same with the surgery percentage of recurrence in early period of observation but faster rehabilitation after interference. Further control clinical study is needed for assessment effect of this treatment in the course of year and more.

In the article the evaluation of the degree of stenotic process in the spinal canal in 48 patients with stenosis of the spine judging by the morphometric measurements of vertebrae, dural sac and spinal canal. The authors improved and supplemented the morphometric technique of measurements of a number of parameters, on their basis the mathematical model was worked out and mathematical calculations to determine the coefficient of stenotic process in the spinal canal at the lumbar level were hold (Zn). The diagnostics of stenosis of spinal canal basing on worked out clinic ray criteria using mathematical modeling can help more complete diagnostics and evaluation of the degree of stenotic process of the spinal canal at the lumbar level and let choose appropriate prevention and treatment of this pathology. The calculated ratio of stenotic process (Zn) and its values let clinicians choose tactics of conservative or surgical treatment in time and choose the way of prevention of complications of stenotic process of the spinal canal.

The study included 287 patients with tubal-peritoneal infertility after unsuccessful attempts of in vitro fertilization (IVF). All women, from 5 to 8 days of the menstrual cycle performed hysteroscopy with taking material for histological and cultural study of the endometrium, extended colposcopy (CPS). Endometrial pathology is not detected by ultrasound before IVF and Paypell biopsy was detected in 252 (87,9 %) patients, chronic endometritis prevailed in 78,7 %. The etiological structure was determined with a predominance of opportunistic microflora and mixed infection had almost all patients. 196 (68,2 %) patients had colposcopic signs of chronic inflammation of the cervix. Pregravid preparation for assisted reproductive technology (ART) should include hysteroscopy, followed by causal therapy.

The study included 30 reproductive age women with lower female reproductive tract nonspecific inflammatory diseases. The analysis of the qualitative and quantitative pathogenic microorganisms as well as lactobacilli composition was conducted. It was shown that in women observed the opportunistic microflora detected often (73,3 %) in the rest part of patients (26,7 %) in case if opportunistic microflora was absent the disbiotic changes occurred in lower concentration of normal microflora of vagina represented mostly by lactoflora. Deficiency of lactobacilli was observed almost in all women of this group (96,6 %) and only in one case (3,4 %) the concentration of lactobacilli was consistent with normal physiological range. Molecular genetic methods (PCR amplification) with visualization by means of electrophoresis in agarose gel was made for identification of Lactobacilli species. Consistency index (c) and species saturation index (sri) for opportunistic microorganisms in women vaginal biotope examined were calculated. Prevalence of Lactobacillus plantarum and Lactobacillus jensoni isolating in 83 and 50 % respectively was shown. Also it was shown that in the structure of quantitative compatibility of studied species of lactobacilli the highest rate is characterized to the association of two types that makes 53 %.

In this paper the microecological characteristics of Klebsiella spp. regional populations in the intestinal microbiota of infants were represented. 373 children (55,9 % of general quantity of researched) had disbiotic changes of intestinal microbiota related to increasing number of Klebsiella. The autostrains sensitivity to antibiotic drugs (в-lactams, aminoglycosides, quinolones) and to purified Klebsiella pneumoniae phage and purified polyvalent Klebsiella phage were researched. Samples of isolates (n = 142) characterized by a high proportion of antibiotic susceptible strains. Phage sensitivity of bacteriophage to the commercial preparations was determined in 268 strains of Klebsiella spp. of two types (146 strains of K. Oxytoca and 122 strains of K. pneumonia). Probably high frequency of pathogens resistant to studied bacteriophages (K. oxytocain 66 % and K. pneumoniae in 77.8 % of cases) was the reason of low efficiency of phage therapy by specific phage and can explain high frequency of occurrence of Klebsiella at dysbiosis in children of early age.

The results analysis of studying the adaptation mechanisms of the cardio-respiratory system to the extreme conditions of Far North are represented in this article, as well as the preventive measures which may reduce their unfavourable effects are suggested. The cold and low content of water vapors in the atmosphere which are characteristic to the high latitudes and which may decrease in the gas metabolism efficiency in the respiratory organs and may be considered as the leading link of the respiratory organ changes are known to be the mainfactors of the natural environment which may influence on the functional state of the human organism. The organism adaptation process features to the extreme weather conditions of Far North are known to consist in the morphological and functional changes in the respiratory organs, in particular, in increase in the area of the alveolar lung surface on the average by 24 % and the lung capillary volume - by 39 %. The increase in the systolic pressure in the lung artery higher than 30 mm of mercury column is also known to be process feature of the adaptation to the extreme weather conditions of Far North. This may form the clinical-morphological complex specific to the high latitudes. The adaptation to the extreme climatic conditions of North was found to run via some stages. The increase in the functional reserves and the area of the gas metabolism due to the additional alveolar uncovering in the upper and middle lung zones may occur during the period of the initial adaptation to the cold. During the long-term residence in North (more than 10-15 years) the capability of the further increase in the adaptive organism reserves is known to be exhausted and the increase in the gas metabolism area may be observed mainly due to the morphological changes. The gas diffusion disorder via the alveolar-capillary lung membrane may promote the development of hypoxemia, to the decrease of which all the compensatory mechanisms of the respiratory system are finally activated. The long-term hypoxemia which may be aggravated during the cold period was found to increase in the free radical process and to decrease in the content of the organism antioxidants. That's why, the large physical overloads, smoking, the cold may lead to the aggravation of the hypoxemia and to the increase in the pathological changes in the lung tissue which in turn may stipulate the after appearance of the acute and chronic diseases of the respiratory organs which may be very painful. Even for the practically healthy residents of Zapolarya the occurrence of the short-wind and increased tiredness in performing the daily activity may be typical. Thereby, the function change of the external respiration is characteristic to both the native population of North and to the migrants. As the preventive measure, performing the short-term courses of taking the high does of food antioxidants during the winter period, keeping the optimal temperature and moisture regimes in the living and production rooms as well as using the means of the individual protection for the respiratory organs are suggested.

There is represented the analysis of the sickness rate among the children and adolescents on the typical areas of the Irkutsk region during 2003-2011 (by example of the Taishet district). We have established that the sickness rate of the Taishet district is typical for every south-west area, however significantly lower than in industrial centers of the Irkutsk region. Children and adolescents of the whole Irkutsk region as well as the Taishet district distinguish themselves by the increase of the sickness rate of respiratory diseases (the growth rate over the period of the analysis ranges from 18 to 117 %). There are calculated the values of the comparative risk of the priority types of diseases for industrial centers: respiratory diseases (for children RR = 1,4 (р < 0,05), for adolescents RR = 1,22 (р < 0,05)); injuries, poisonings and accidents (1,8 (р < 0,05) for both age groups).

Microbiological monitoring of the six districts of the Krasnoyarsk Territory was conducted in order to prove the existence of natural foci of human granulocytic anaplasmosis (HGA) and monocytic ehrlichiosis (HME) on its territory. Two-round PCR method was used for the detection of Anaplasma and Ehrlichia DNA. 374 samples from mites were analyzed with two-round PCR for the presence of Anaplasma and Ehrlichia DNA: 341 specimens of Ixodes persulcatus and 33 specimens of Haemaphysalis concinna. Ticks were collected from vegetation on the flag in the 6 districts of the Krasnoyarsk Territory: Yeniseisky, Achynsky, Sharypovsky, Ilansky, Minusinsky and Ermakovsky, belonging to different climate zones and have landscaped, geo-botanical and faunal features. The study showed that 5.6 % of taiga ticks infected with E. muris, 2,1 % with A. phagocytophilum. In 0.3 % of carriers we identified mixtinfected E. muris and A. phagocytophilum. These data indicate presence of HGA and HME foci in the central, western and southern parts of the Krasnoyarsk Territory.

Shiga toxins (stxs) are the virulence factor of enterohemorrhagic Escherichia coli (EHEC). Shiga toxin-producing Escherichia coli has been recognized as an important food-borne pathogen that causes severe diseases such as a hemolytic uremic syndrome (HUS). Provides information about the species composition of the major opportunistic organisms that inhabit this biotope. Using polymerase chain reaction (PCR) 96 strains of E. coli were examined for the presence of genes stx1 and stx2 coding the ability to toxigenesis. They were isolated from the children with functional disorders of the gastro-intestinal tract. Both pathogenicity genes present in the genome of E. coli with normal enzyme activity more often (stx1 in 24,2 % of genomes, stx2 in 9,1 %). The presence of stx1 and stx2 genes in different biochemical variants of E. coli allows to ascertain the fact of presence of a potentially pathogenicity reservoir in non-pathogenic forms of E. coli. Intestinal microflora is integral part of each individual. Further studying of its functions, the states interfering qualitative and quantitative composition of microorganisms, colonizing human gastrointestinal tract, as well as the pathogenic potential.

The information about more than 17 thousand cases of public inquiries to the Center of Diagnostics and prevention of tick-borne infections of Scientific Center of Problems Family and Human Reproduction Problems because of bites of ticks at the territory of Irkutsk Region during the last six years (2007-2012) is analyzed in the article. All age groups undergo to the bites of ticks. Over the half of the people asking for help have the policy of voluntary health insurance of "Anti tick" kind. The percentage of people vaccinated against the tick borne encephalitis is a little bit over than 8 % what proves the low level of the sanitary education and neglect to the human health in the region. Every year the cases of tick's bites and findings of tick-borne encephalitis virus antigen in blood serum of bitten people in the northern areas of Irkutsk region are revealed. Every year in average 90 examples of blood serum had tick-borne encephalitis virus antigen that means 3 % of bitten people have pre-clinical infection of tick-borne encephalitis. Patients with positive result of tick-borne encephalitis virus antigen (543 patients) had the necessary consultation and preventive treatment with specific immunoglobulin according to the instruction of application. Diseases of tick-borne encephalitis among the patients had the preventive treatment in time were not marked.

Environmental conditions may be a prerequisite for the development of various pathological processes in individuals living in environmentally disadvantaged areas. The respiratory system is the first target of the pollutants. Therefore, respiratory diseases occupy the first place in the overall morbidity. The aim of this work was to study the content of autoantibodies in blood serum of teenagers with chronic disease of the upper respiratory tract, living in air pollution emissions of the petrochemical industry. Teenagers from industrial center living near petrochemical company and "sleeping areas" were examined. Otolaryngologist conducted a survey to assess the state of the upper respiratory tract (URT). In each of the areas individuals with and without chronic diseases of the URT were isolated. The content of IgG antibodies in the serum to antigens that characterizes the general state of the immune system (the native DNA, p2-glycoprotein I, Fc-fragment of IgG) was studied by enzyme immunoassay. Statistical processing of the results was performed using nonparametric methods software package «Statistica 6.0». Diseases of the respiratory system take the first place (42.9 %) in the structure of morbidity level. According to anamnestic data more often were the following disorders: chronic tonsillitis (18,9 ± 2,6 %), chronic pharyngitis (6,8 ± 1,7 %), acute respiratory infections (42,3 ± 3,32 %). Comparison of the incidence of chronic diseases of the upper respiratory tract infections in teenagers found that cases of chronic tonsillitis were observed in 45,0 ± 4,9 % of schoolchildren living near the industrial plant and 33,3 ± 4,3 % of teenagers from residential areas, cases of chronic pharyngitis - in 12,7± 3,3 % and5± 1,9 %, respectively. In teenagers from the «sleeping areas» with chronic diseases of the URT the level of auto-antibodies to p2-glycoprotein I was lower than one in healthy children from the same area due to the greater number of cases of lower values. In the group of teenagers with chronic diseases of the URTfrom environmentally disadvantaged areas children with high content of antibodies to p2-glycoprotein I were more common than in the groups of healthy teenagers living in the same area. The findings suggest that there is activation of the immune system in healthy schoolchildren living in a «sleeping areas" of the industrial city. It can be assumed that the detected changes in the content of autoantibodies may indicate features for the adaptive processes under development pressure because of the surveyed students identified isolated cases of hypo- or hyperimmunoreactivity of specific autoantibodies.

New data was obtained about the original version of tickborne encephalitis virus (TBE), circulating in the territory of Eastern Siberia. With the help of molecular hybridization of nucleic acids (MGNA) and genome sequencing we revealed a group of 13 strains having a genetic structure analogous to strain 886-84, which was described earlier as the sole representative of the probable genotype 5. The formation of a separate cluster on the phylogenetic tree, differentiation level of genetic differences from other genotypes more than 12 %, own habitat, ecological relationship with all the links of a vector-borne chain, participation in human pathology, the stability and duration of circulation in nature confirms the validity of certification of «group 886» as a separate TBEV genotype 5.

This article contains results of reconnaissance studies intended to find foci of babesia in the Irkutsk region. Babesia DNA were founded in two types of ticks (I. persulcatus, H. concinna) in the three districts of the Irkutsk region (Ekhirit-Bulagatsky, Irkutsky, Shelekhovsky). The analysis of 1220 nr length nucleotide sequences of 18S rRNA gene of five Babesia samples was conducted. It was shown that the identified Babesia sequenced differ from previously known, and refer to the two genetic groups. The first group included babesia, genetically most similar to the sheep pathogen Babesia crassa, second contains sample, nucleotide sequence of which is significantly different from all known (less than 95 % homology).

The content of fluorine in atmospheric air, in soil, and in system of centralized household-drinking water supply of city of Bratsk are studied, data of branch of Federal Budgetary Healthcare Institution «The Center of Hygiene and Epidemiology of Irkutsk Region» in Bratsk and region of Bratsk, data of Bratsk branch of state institution «Irkutsk CGMS - P» are used. The assessment of prevalence of iodine deficiency of children in two ecologically various districts of city of Bratsk is given. Results of obtained data are turned into practical recommendations on prevention of iodine deficiency in conditions of industrial influence of fluorine. In chosen areas examination of children of preschool age is provided. The number of surveyed made 98 persons including 49 girls and 49 boys. Iodine and fluorine excretion with urine, and also content of fluorine in teeth were defined. Iodine in urine was determined with a spectrophotocolorimetry method. The content of fluorine in urine was determined by an electrometric method with the use of fluoride selective electrode complete with pH in meter according to TORMENTS 4.1.773 - 99. Approved, certified methods of definition of fluorine in teeth do not exist in Russian Federation. Fluorine definition in teeth is based on reaction with alizarin complexon and cerium nitrate with formation of the painted connection. Now it is the only reaction used for direct photometric definition of fluorine. We came to conclusion that contents of fluorine in such biosubstrates as urine and teeth in children of the Central district, though doesn>t exceed admissible level, is significantly above the one of children living in settlement Energetic. This results from the fact that atmospheric air in the Central district is more polluted by fluoric connections. It is known that iodine and fluorine are halogens, and the second one possesses more expressed biological activity and can replace the first in the competitive way. Thus, in this research we tried to prove experimentally that urinary iodine indicators directly depend on industrial influence of fluorine.

Aim. Zymographic analysis of water-soluble proteases of Vibrio cholerae O1 и О139 serogroups. Materials and methods. Bacteria cultivated on a casein-yeast agar (рН 7,6) at 37 ° with during one twenty-four hours and washed off physiological solution. Bacterial mass (by the concentration of 109 cell./ml) was treatment by sterile solution of urea in an eventual concentration 4,5 М. After day's display and determination of sterility of got lysate insoluble in urea material (cell walls) was deleted by high-speed centrifugation. Supernatant liquid was exposed to the dialysis, released from insoluble in water sediment centrifugation and freeze dried out. Protease activity was determined in diffusion test in 1 % agarose gel, containing 0,5 % gelatin or 0,5% casein. The spectrum of proteases was analysed by a substrate electrophoresis in the blocks of 8% polyacrylamide gel, impregnated in the process of polymerization gelatin or casein (in an eventual concentration 0,1 %), in presence the dodecylsulphate of sodium. Results. After differential centrifugation of ureal lysate of cells and dialysis there are mainly intracellular water-soluble proteases in the investigated extracts. Diffusion tests showed that all preparations of the investigated strains of Vibrio cholerae possessed protease activity of different intensity. Changing of substrate in diffusion test from gelatin to the casein resulted in the general diminishing of the registered areas of hydrolysis, specifying that a casein fissions less actively by comparison to gelatin. Substrate electrophoresis showed that proteins, making the spectrum of water-soluble pro teases of Vibrio cholerae are extracted by urea, possessed molecular mass, varying within from less than 30 кйа to more than 120 кDа. Strains distinctions are marked in quantitative and high-quality description of spectrums of soluble proteases. Dependence of description of type of water-soluble proteases is educed on used substrate. At the estimation of spectrums of proteases clear confirmation is got, that on gels are impregnated by gelatin electrophoretic mobility of proteases is higher, than in gels, containing a casein. Conclusion. Substrate electrophoresis of preparations of cell-free lysates of Vibrio cholerae showed the presence of a few water-soluble proteases, quantitative and high-quality interstrain distinctions, dependence of spectrum of active proteases from of used substrate.

Thirty-one isolates of M. tuberculosis was identified in Buryatia by MIRU-VNTR-24 typing and deletion analysis by RD105 and RD207. Beijing family strains accountedfor 67,4 % and had a variety of profiles with a prevalence (22,6 %) of two modern epidemic subtype Beijing MIT17 and MIT16. Strains Beijing MIT 642 differed in the number of repeats of the variable loci, it may reflect a longer circulation of these subtypes without shaping their properties of epidemic circulating strains in modern populations, or they had epidemic importance in the past. It is also apparent heterogeneity in the number of loci among strains according to group LAM, T, and X, which requires additional research. The aim of this work was to determine the current epidemiologically important genotypes of Mycobacterium tuberculosis, as well as the genotypes had an epidemic importance in the past as an example of the region with a high prevalence of tuberculosis - the Republic of Buryatia.

The model of multiple skeletal injuries was reproduced in two groups. 22 animals of the 1st group had stabilization of the right forearm bones and left shin with spoke external fixation device of 2 subsystems with followed osteotomy of the bones of respective segment in the middle third under general anesthesia. During the experiment the injection of dexamethasone and adrenaline during the first twenty-four hours after multiple skeletal injuries prevented the reduce the intensity of the metabolic processes, that was proved by close to initial values of oxygen consumption and carbon dioxide emissions. But to the third day there was no any difference in the metabolic activity in rabbits having and not having adrenaline and dexamethasone. The animals had adrenaline and dexamethasone turned to be in more severe clinical situation. As a result of «normal» intensity of metabolic processes in rabbits during the near time period after trauma there was decrease of survival from 45,5 % to 7,7 %.

In experiment the forming and remodeling of distraction regenerate in rabbits Shinshila while conducting transosseous elements through the acupuncture points were studied. One of the rabbit's forearm was stretched by Hizarov's method for 10 mm. Multidetector CT gave the results of mineral density of the bone distraction regenerate. To the 10th day of fixation of bone fragments by the external fixation device the distraction regenerate with a clear zonal structure formed. To the 20th day of fixation in the middle zone there were clear signs of the formation of the medullary canal, that proves the decrease of mineral density to negative values. The prolonged stimulation of acupuncture points by introduced transosseous elements leads to formation of the structure and properties of bone regenerate, which are close by the characteristics to the parameters of the intact bone.

The participation of Se in the structure of deiodinases during the metabolism of thyroid hormones defined the interest to the research of its possible impact to the processes of reparative regeneration of bone tissue in the experiment. The aim of the paper is to reveal the dynamics of thyroid hormone secretion at further adding of selenium under the conditions of standard fracture healing in rabbits. The most general response of the thyroid gland at the reparative regeneration of bone tissue was revealed, it is the reduction of Т, T₄ and free T₄ concentration during the nearest postoperative period. It is shown, that the use of the preparation of Se changes the reaction of thyroid to the dosed damage of the bone and metabolism of iodothyronines as well. The change of T₃ concentration was the most evident. Rabbits having extra preparation of Se were less susceptible to fluctuations in the secretion of iodothyronines typical for the dosed damage of the bone but the syndrome of «low Т₃» redoubled and peripheral conversion of thyroid hormone reduced.

The purpose of this study was to determine the effect of herbal means «kardekaim» on indicators of physical endurance. Actoprotective activity was determined by the general models, high-speed and power of physical endurance in experiments on white mice. Overall physical endurance was determined by the duration of the swimming pool in mice with a load, speed of physical endurance was assessed by running the length of the mice in the 10-track treadmill, strength endurance were evaluated by retention time on a pole mice. Found that the introduction of rnurse «kardekaim» increases overall, speed and power physical endurance of laboratory animals against peace. On the model of single-and multiple-dose exercise kardekaim increases the potential for physical performance, prevents fatigue and speeds up recovery of physical endurance of animals undergoing high intensity exercise. The most effective kardekaim shows at course introduction. It is proved that the effect of the introduction at course «kardekaim» superior product comparison - Eleutherococcus extract.

Seeing the formation of new system pathology of musculoskeletal system under the condition of man-made pollution of the habitat of the population of Eastern Siberia the authors suggest the creation of new organizational forms of detection and treatment of this pathology because it promotes prevention and reduction of the disability level in children and teenagers. Teenagers of 15-18 years old don't have opportunity to get specialized inpatient orthopedic and trauma care in hospitals of Irkutsk region. In it's turn early prophylaxis of disability of children and teenagers with pathology of musculoskeletal system has not only medical, social but economic efficiency as well. Saving a decent quality of life has a humanitarian significance for great part of children population of Irkutsk region. The creation of efficient programs of rehabilitation needs understanding of general mechanisms of pathological process development. Noninvasive methods of diagnostics let to reveal the disease in early stages.

This article represents the result of 10 years work of scientific stuff going into researching of degenerative diseases of the spine. The new theoretical knowledge taken to the basis of new ways of diagnostics, forecasting and treatment was obtained. The introduction of suggested new technics of diagnostics, treatment and forecasting of pathologies of the spine let to improve results of treatment of patients with spinal stenosis, postoperative adhesion scar-epiduritis, herniated intervertebral discs, instability of the spinal motion segment. Finding out the regularities and mechanisms of multifactorial syndrome of instability was taken as the basis of working out by scientists pathogenically founded ways of correction of this syndrome. All new methods are patented and adopted by new technologies.

As a result of the analysis of literature it was revealed that in case of pronation-abduction ankle injury takes place injury of the articular surfaces. The hyaline cartilage significantly suffers. Typical localization of damage of the articular cartilage is the middle or front third of the inner edge of talus block. The damage can spread to articular surface of the outer block of the talus. In 50 % cases the articular cartilage is the front edge of the tibia is damaged. The combination of damages of the articular cartilage of the inner edge of the block of the talus and the outside the department anterior edge of the tibia is more often (from 43 % till 57 %). In 80 % cases the damage of the articular cartilage surface of the talus ankle as roughness, cracks, defects with exposure of subchondral bone was revealed. The results say that in case of damage bony and ligamentous structures of the ankle at the joint pronation-abduction mechanism the articular cartilage significantly suffers. The size and character of the damages of the articular cartilage differs. i4s a result we can say that initially severe damage of the articular cartilage at trauma will significantly worsen the immediate and long-term results and this fact should be taken into account while making plan of the treatment of patients with ankle injury.

This paper presents a review of the scientific literature on the pathogenesis, diagnosis and treatment of pneumonia in liver cirrhosis. Pneumonia occur in 4-21% of patients with cirrhosis of the liver, the most common etiology of alcohol. Often the development of pneumonia complicated by bleeding from varicose veins in the esophagus. Mortality rate is 40%, the risk factors of mortality are impaired consciousness, mnogodolevaya pneumonia, ascites, acute renal failure, bacteremia, length of hospital stay. Separately considering other options lung disease in patients with liver cirrhosis (hepatopulmonary syndrome and portopulmonary hypertension), as well as other causes of fever in liver disease. An algorithm for investigation of patients with abnormal liver function, and fever, which includes finding the source of infection, diagnosis of bacterial overgrowth syndrome, bacterial translocation and endotoxemia, and consideration of possible non-infectious causes of fever.

The modern findings of scientific research in a live plague vaccine range such as production antibiotic-resistant subcultures of the vaccine strains, design of the low-development of reaction producers vaccine strains, production of oral (tablet) preparation are represented in the review. Some techniques of production improvement live plague vaccine are formulated: stabilization of a number alive microbial cells in a inoculation dose of a license vaccine by decreasing of cultivation temperature mode from 27 °C till 21°С, concentration of microbial cells in ampoule from (5-10) × 1010 till (1-4) × 1010 per 1 ml, reduction freezing-drying step from 28 till 18 h, decreasing of amount dose in ampoule from 100-125 till 20-50 and furthermore acceleration of production process of microbial cells concentrate (fermentation in the bioreactor, tangential microfiltration) and formulation combined (meat-soya nutritious base) medium and conditions of their preparation (time reduction enzymatic hydrolysis from 14 till 3 days). The tendenceis of refinement of a technological process of equipment link were researched, such as using bioreactors instead of apparatusfor a cultivation microbesShesterenko, disposable bags Flexboy, which are allowed to eliminate the application of glass bottles and operation of a bottle assembling, also using of robotizing sublimation installations for drying of a liquid form of live plague vaccine, which are allowed to exclude utilization of low-temperature refrigerators. Beside of regulating estimation method of immunogenicity (calculation of effective immunizing dose) and quantitative account of a phenomenon of survival white mice the cyto-enzymochemical and morphometric rates are suggested to estimate the quality of ultimate product live plague dry vaccine, changes of those are charactering immunologic activity and state of mobilizing and bactericidal abilities of a organism phagocyte system cells.

We conducted the microbiological examination of vaginal microbiota in 324 women with non-specific inflammatory diseases of the genital tract and with reproductive dysfunction. According the condition of microecological balance of indigenous microflora (lactobacilli concentration) of women we have identified three types of vaginal microbiota: "normocenosis", " dysbiosis", "deep dysbiosis" We marked deficiency oflactobacilli in every fourth women (28%) with infertility and miscarriage, and deep deficit in every second (53% in the third group) - sharp depression of the indigenous microbiota and its replacement by opportunistic (facultative anaerobic and obligate anaerobic), what indicates microecological trouble in the vaginal biotope and can be one of the causes of infectious and inflammatory diseases of genitals and other reproductive disorders. It was shown that the dominant microorganisms of pathogenic microbiota (UPM) of inflammatory diseases in women are coagulase-negative staphylococci (CNS), fungi of genus Candida, Escherihia coli and Enterococcus spp. During the analysis of UPM representatives material of groups with "normocenosis", "dysbiosis" and "deep dysbiosis" an important microecological indicator was calculated which characterizing microbiocaenosis as a whole - index of species richness (ISR) - the average number of species in the composition.

In the last decade outbreaks and epidemics of infectious diseases dangerous for humans cause tension epidemiological situation in the world and in the Russian Federation. Epidemic manifestation of these diseases has symptoms of emergency situations and requires the use of additional forces and means of Public Health, as well as administrative, legal and financial support. The purpose of this study is formulation of the concept of "public health emergency situations associated with diseases dangerous for humans". In considering of definitions "emergency situation", "disease" and "event" in international and national law, the thesis is confirmed that epidemic manifestation of infectious diseases dangerous for humans, are signs of an emergency. In addition, the interpretation of these terms focuses on the distinction between potential risk and its actual implementation. Consequently, the threat of public health emergency situations should be viewed from the risk methodology, by which we proposed to evaluate internal and external epidemiological risks and to define its dominance for each municipality of the Russian Federation Region. Infectious diseases dangerous for humans which able to cause public health emergency situations, can be divided into three groups. 1. Diseases that form the external epidemiological risk - dangerous infectious diseases requiring measures for sanitary protection of the territory of the Russian Federation. 2. Diseases that form the internal epidemiological risk - dangerous infectious diseases, registration both single and mass indigenous events requires emergency response and using of additional forces and means of public health of the Region. 3. Infectious diseases of importance in regional pathology, prevention of which is to implement long-term programs. In the framework of this paper the definition of "public health emergency situations associated with diseases dangerous for humans" is proposed. It focuses on the feasibility of upgrading approaches to priority directions of anti-epidemic readiness of the Russian Federation Region, taking into account the probability of epidemiological risks.

The article discusses the phenomenon of metabolic adaptation to ischemia after repeated episodes of transient reduction in myocardial perfusion. It is shown that the phenomenon of ischemic preconditioning is to significantly improve the stability of the ischemic tissue and reperfusion injury that occurs after a few brief episodes of ischemia-reperfusion. Thus ischemic preconditioning is a multifactorial phenomenon, as implemented through adaptation to changes in the intracellular cascade of metabolic conditions and the restructuring of the myocardium with increased tolerance to its lower perfusion. The use of external actions aimed at the individual links of the process or set of them, will improve the outcomes of ischemic episodes, and better preservation of myocardial function in a compromised vascular bed. It is shown that one of the major incentives of ischemic preconditioning may make growth factors - FGF2 and VEGF. In this case, the protective effect is realized as by increasing the viability of myocardial contractile cells and vascular endothelial cells in myocardial ischemic damage of the hearth, and by neo-angiogenesis and improve the regenerative ability of mature cardiomyocytes.