The article presents the results of use of various fractal methods and their author modifications applied to the automated recognition of sleep phases by computer electroencephalograms. The results of the application of the Grassberger-Procaccia method, Hurst rescaled range method, the author's modification of the method of false nearest neighbors and. the method of approximating entropy for recognition of sleep phases at polysomnography are presented in the work and. compared by relative rate and. accuracy. Brief description of peculiarities of used fractal methods is given in the article. It was showed that use of fractal methods of determinate chaos in synergic approach allows to realize differentiation of sleep stages with relatively high accuracy at significantly reduced time limits with use of only one canal of encephalogram. Reliability of obtained results was estimated. by comparison with the results obtained by certified neurophysiological specialists

The aim. of the work was to create universal protocol of realization, of some restorative method, depending on the type of trauma of holding structure at surgical treatment of rectal cancer. The researched group of patients included. 1499 patients with rectal tumors who had. radical surgical interventions during 2006-2010 years. All patients after the operations were divided into the groups depending on the severity of pelvic diaphragm injury. Consequence of methods of reconstruction of holding structures depending on the degree of injury of anorectum was developed. The choice of method of operative intervention and. way of reconstruction of elements of pelvic floor is the result of introduction of complex of individual navigation surgery when the surgeon at preoperative stage offers use of consequence of technical approaches that was developed individually for each patient taking into account anatomical features, localization and stage of tumorous process

The aim. of work was to study the relationship between structural and functional changes in the myocardium and the parameters of heart rate variability in patients with chronic prostatitis. The study included 51 patient with chronic prostatitis of average age 34,2 ± 8,1 years. The study excluded patients older than 45 years, with various heart diseases, arterial hypertension and a history of inflammatory diseases of any other localization. Echocardiography research was carried out by standard methods on the device "VIVID-3-expert GE". All the patients had. Holter ECG monitoring with analysis of heart rate variability. In patients with chronic prostatitis the disorder of vegetative cardiovascular regulation of cardiac activity in the form of hypersympathotony and. significant reduction of protective vagal activity was revealed. Main differences of structural and. functional parameters of myocardium related to such indices as the thickness of the posterior wall, interventricular septum, left ventricular myocardial mass, time, isovolumetric relaxation of the left ventricle, which in patients with diastolic dysfunction of the left ventricle and. left atrium dilatation significantly higher than those settings intact myocardium of the left ventricle and. atrium, as well as a control group. The lowest values of heart rate variability reported in patients with structural and. functional changes in the myocardium of the left atrium and. diastolic dysfunction of the left ventricular. Thus, one of the mechanisms of left ventricular remodeling in patients with chronic prostatitis, a vegetative imbalance with sympathotony associated with lower basic spectral, temporal and geometry of heart rate variability. Prolonged activation of the sympathetic nervous system autonomic managers may directly or indirectly (through the renin-angiotensin-aldosterone system) to initiate and. maintain the processes of excessive growth of cardiomyocytes, contributing to dysfunction of left ventricular relaxation, in patients with chronic prostatitis

The article analyzes diagnostics, surgical treatment and remote results of treatment of 28 patients with cardiac tumors. The most frequently observed cardiac tumor was myxoma of left atrium. (89,3 %). The frequency of cardiac tumors in our investigation was 0,5 % from the total number of cardiac surgeries. Most frequently clinical manifestations were associated with mitral valve obstruction. Echocardioscopy and. transesophageal echocardiography that allow to determine localization, attachment spot, size and character of the tumor in 99-100 % of cases are the basic diagnostic techniques. Cardiac tumor requires urgency of intervention, especially for patients with high risk of embolization at the occurrence of large mobile myxomes with uneven contours and at expressed clinical symptomatology. The most probable causes of death are valve occlusion and. emboly. Removal of cardiac tumors in the early periods after confirmation of diagnosis is an effective operation with good results in the early and remote postoperative periods.

The article presents the results of treatment of patient M. after traffic accident who had. open fractures of both shin bones complicated with local and disseminated form of chronic traumatic osteomyelitis. At the treatment of patient authors' methods were used. On the left shin to stop local necrotic-purulent process we used monolocal compression osteosynthesis and. carried out extra operation - extra-focal autotransplantion. On the right shin to restore integrity of tibial bone at disseminated process we carried out bilocal compressive-distraction osteosynthesis and. used method of bone defect replacement of individual rate. At the result of treatment we managed to restore support ability of low extremities and. to achieve stable remission. of osteomyelitis process. Use of new methods of treatment allowed to join the fracture of bones of one shin and. to replace spacious bone defect of another in quite short terms at the osteomyelitis process.

60 men of 25-39 years (10 healthy men and 50 men with nasal septum deviation (NSD)) had daily Holter monitoring of electrocardiogram. Patients with. NSD who had septoplasty were divided into groups: without anesthesia, analgin, diclofenac, ketorol and ketamine - 1^st, 2^nd, 3^rd, 4^th and 5^th groups correspondingly. Healthy people were related to the group 0 (comparison group). Monitors in 1^st-5^th groups were set 30 minutes before and. operation and. were took away in a day. Circadian index (CI) was calculated by the deviation of ST segment from contour (CST) and. by heart rate (HR). Approximation analysis of CI was realized, within each group, Student analysis was realized between the groups. Approximation coefficients were from 0,6 to 0,87. Differences of circadian indices on CST and. HR were revealed only in the 1^st group (p < 0,05). Intergroup analysis showed that groups 0 and. 3 were comparable, decrease of CI was revealed in the 4^th and. 5^th groups (p < 0,05). The 2^nd group had tendency to the decrease of CI. CI can be calculated non only by HR but also by deviation of CST that points to the significance of CST not only as a marker of ischemia but also as the index of tension of vegetative nervous system.

The study included. 173 children with minimal brain dysfunction. (MBD) at the age of 2-8 years and 90 healthy children comparable by age and sex. As the result of dynamic examination we revealed two types of clinical course: favorable, showed as a regredient variant, and. unfavorable type, showed as a regredient variant with light residual organic cerebral insufficiency and. progredient variant. Favorable type of MBD course was observed in 70.3 % of children of pre-preschool age and. only in 22,2 % of children of the early school age. Unfavorable type was is 2,5 times more often in the group of children of early school age. Prognostically important risk factors were determined, by discriminant analysis, the degree of their mutual influence both on the development and. clinical course of MBD were defined. A system for individual prediction of development and. clinical course of MBD that allows revealing the children with risk of appearance of the disease with 95,4 % accuracy was developed. Determination of type of clinical course of MBD with 98,2 % accuracy gives the chance to carry out individual treatment and. preventive measures, including the correction of the most significant "controlled" risk factors in proper time.

The article is based on the analysis of the results of retrospective examination of 182 patients who entered the clinics of SCRRS SB RAMS for surgical treatment of diaphyseal fractures of shin bones, complicated with chronic traumatic osteomyelitis (CTO). The estimation of the results of traditional surgical treatment with local form of CTO at the application of biolocal osteosynthesis was realized. At the traditional surgical treatment of patients with shin bones fractures complicated with CTO long terms of adhesion of fractures and replacement of bone defects, prolonged course of inflammatory process in the zones of resection of a bone and. of transosseous elements. In 77, 5 ± 3,52 % of patients use of traditional treatment causes slow adhesion of the fracture, formation of false joint and. poorly mineralized distraction regenerate. In 11,2 ± 9,35 % of patients osteomyelitis process isn't managed to stop.

Conditions of environment can become causes of developments of different pathologic processes in people, living in ecologically unfavorable regions. The aim. of the work was to study contents of autoantibodies in blood serum of teenagers with chronic pathology of respiratory tract living in areas contaminated with emissions of petrochemical industry. We examined teenagers living near petrochemical industry and. in commuter towns. In each area people with and. without chronic pathology of respiratory tract were marked out. We studied the levels of serum autoantibodies to antigens that characterize condition of immune system. (nDNA, β2-glycoprotein I, Fc-enzyme, IgG). The changes of autoantibodies contents can tell about peculiarities of course of adaptive processes in teenagers living in the conditions of technogenic load.

The objective of the work was to study value of indicators of pyridinoline and dezoksipyridinoline in the prognosis of development of slow consolidation of fractures of long bones of extremities. We carried out the research of markers of resorption of bone tissue in 75 patients of 16-55 years with fractures of long bones of extremities in postoperative period. The first group included. 44 patients with non-complicated course of posttraumatic period. The second group (n = 31) included patients with disorder of consolidation of fractures in late posttraumatic period (slow consolidation). Control group included. 30 nearly healthy people of 18-35 years. Groups of patients were compared by sex, age, time of getting the injury, character of fractures and. the treatment they got. The criteria of excluding out of the research were patients with any concomitant pathology and. also women in menopause. The researches were realized on the 2^nd and. the 10^th day of posttraumatic period. Statistical processing of the data was realized with help of «BIOSTAT». The analysis of data between the groups of patients in different terms of posttraumatic period was carried out with, help of Newman - Keuls criteria. To compare indices of patients with, complicated and. non-complicated. course of fractures of long bones of extremities we used Mann - Whitney criteria. The differences were considered statistically sigificans at p < 0,05. It was determined that at the level of pyridinoline in blood serum more than 15 % and of deoxypyridinoline in urine more than 35 % of control values on the 10^th day after trauma the disorder of consolidation of fractures in late postoperative period is prognosed. Determination of indices of pyridinoline and. deoxypyridinoline patients with fractures of long bones of extremities on the 10th day of posttraumatic period allows to prognose development of slow consolidation of fractures that can allow to realize preventive prophylactic and. treatment measures aimed at precaution of development of disorders of consolidation.

The research is based on the analysis of treatment of 30 patients (during 2002-2010 years) with. Monteggia injuries by transosseous osteosynthesis. The aim. of the work was to show opportunities of transosseous osteosynthesis as mini-invasive method that allows to restore anatomical structures of injured segment by closed method. Separate fixation of radial and ulnar bones used in the work allowed to remove fixator from the radial bone without losing rigidity of fixation of ulnar bone and. to provide the opportunity of earlier development of rotation. At the estimation of effectiveness of treatment we considered functional activity of injured upper extremity in addition to the result of clinical and. radiologic examination of the patient. The accent was made on the typical mistakes at the treatment of these patients. Study of outcomes of treatment showed that 13 patients (86,6 %) had. usual degree of social integration in 6 months, 2 patients (13,4 %) had. slight limitations of social life. In a year all patients were considered socially integrated.

In the study carried out in primary population of Kemerovo region - teleuts - we determined the prevalence of toxic, compression-ischemic (tunnel), diabetic and atherosclerotic polyneuropathy. It was determined that the frequency of these diseases is different depending on the sex, age, social status, profession, and place of residence. In male population toxic polyneuropathy prevails in age groups of 30-39 and. 40-49 years. Compression-ischemic polyneuropathy prevailed in women of 50-59 years who belonged to the unemployment and. employees social groups. Diabetic and. atherosclerotic polyneuropathy has the most prevalence in women of 70 years and. older in the social group of pensioners.

The aim. of the work was to introduce implants with bioactive coating in the practice of treatment of long tubular bones and. to assess speed of consolidation in comparison with bioinert constructions with use of clinical data, radiation and biochemical methods of examination. Use of bioactive calcium-phosphate coatings obtained by the method of microarc oxidation for the osteosynthesis of long tubular bones is accompanied by more effective consolidation of fracture area in comparison with titanium plates. In 4 months after the operation with use of bioactive implants 95,2 % of patients had. active formation and. formed callus and. only 4,8 % of patients didn't have signs of consolidation. In the same terms at the fixation by bioinert plates adhesion of fracture didn't happen in 24 % of cases. Main mechanism of positive action of bioactive implants is connected with increase of calcium depot in blood and. decrease of markers of bone resorption in the first months after their installation.

Lung paraffin sections of 35 died during the epidemic of influenza A/H1N1 of 2009-2010 in Zabaikalskiy krai were investigated. Exudative stage was diagnosed, in 10 cases, proliferative stage - in 16 cases and fibrotic stage of acute respiratory distress syndrome (ARDS) was diagnosed in 9 cases at the autopsy. Expression of heat shock protein 70 (HSP-70) by cells of the lungs was determined in sections by immunohistochemistry. We revealed that HSP-70 is expressed by neutrophils, macrophages, fibroblasts and alveolocytes type 1 and. 2. It was found that regardless of the stage of ARDS the highest expression of HSP-70 was realized by neutrophils, the lowest - by the endothelium alveolocytes type 2 and. fibroblasts. HSP-70 expression in neutrophils, macrophages, fibroblasts and. alveolocytes type 1 was the same in all stages of ARDS. In alveolocytes type 2 in exudative and. proliferative phases it was lower than at the development of fibrosis. We concluded that one of the mechanisms of developments of acute lungs injury/acute respiratory distress syndrome at influenza pneumonia is comparatively low synthesis of HSP-70 by endotheliocytes of lung capillary and. alveolocytes type 2.

The article is devoted, to the study of secretion of hormones of hypophysis, sexual gland and adrenals in patients with postoperative cicatricial-commissural epiduritis. Concentration of some hormones of hypophysis, sexual glands and adrenals in blood serum of 45 patients with postoperative cicatricial-commissural epiduritis before and. after standard conservative treatment was defined. Typical changes in secretion of studied hormones at postoperative cicatricial-commissural epiduritis were revealed. It was showed that standard scheme of treatment partly grades revealed endocrine disorders. Appropriateness of including research of hormonal profile for solving the problem of its possible correction in the complex of examination of patients with postoperative cicatricial-commissural epiduritis and. of calling endocrinologist for the examination of patients and further enhancement of the scheme of their treatment was proved.

Myasthenia is a chronic autoimmune neuromuscular disease. Antibodies to acetylcholine receptors are found in 85-90 % of patients with generalized myasthenia and play an important role in the pathogenesis of the disease. In the article compared comparison of the level of antibodies to the acetylcholine receptors (AChR) in patients with myasthenia gravis with the severity and duration of disease, age of onset, changes in the thymus and. treatment was realized. We examined. 80 patients. The research of antibodies was conducted by immune-enzymatic method. The highest levels of antibodies to AChR were detected in the group of patients who received long-term cytotoxic agents due to the steady progress of the disease. High. level of antibodies to AChR was observed in patients with predominance of weakness in craniobulbar muscles in clinical presentation. There were no correlations of level of antibodies to AChR with sex, duration of disease, presence of thymoma.

The purpose of this study was comparative analysis of normal biochemical composition of synovial fluid of knee and elbow human joints. Studies were performed on synovial fluid samples of 33 cadavers of suddenly dead people of both sexes (26 men and. 7 women) at the age of 23 to 79 years, without articular pathology registered by expert. We didn't reveal reliable differences of albumen-globulin coefficient, but there were differences in contents of albumen fractions, products of oxide peroxidation of albumens. At the developement of pathological process cast of albumen in synovial fluid is possibly the compensatory reaction in providing of synovia viscosity, because albumen component provides this function on level with hyaluronic acid. Knee joint is an hinge joint, that is meant to bend only in one plane. Biomechanics of elbow joint is much more complicated and. is described as "ginglymoid hinge" with abduction-adduction besides the joint is much smaller. Obtained results can be used as normative values of biochemical synovial fluid indexes in orthopaedic and. trauma practice

The results of ultrasound diagnostics of consequences of traumatic injuries of liver in 122 patients were analyzed. Contours, structure and. texture of intrahepatic formations depended on the type of tissue substrate (haematoma, haemobylema, bylema), terms of formation and infection of traumatic focus. At aseptic course of haematomas and haemobylemas of liver we divided pathological process into 4 stages: stage of latest hemorrhage, stage of formed clot, hemolytic stage and reparative stage. The differences at the infection way of development started from the second week after appearance of intrahepatic injury on the stage of "bacterial lysis of a clot" and. incapsulation. Ultrasound semiotics of intrahepatic bylemas depended only on the infection of contents.

The study of hemodynamics in 199 patients with chronic pancreatitis and pancreatic cancer was performed with use of radiological diagnostics methods: ultrasound research, CT and. portal scintigraphy. Ultrasound examination with color and. power Doppler, CT with 3D-reconstruction give an idea of the local hemodynamic changes in extrahepatic portal hypertension and. can identify preoperative anatomic and. topographic relationship of great vessels in their involvement in mass lesion of the pancreas. Portal scintigraphy with accuracy 83 %, sensitivity 62 % and. specificity 100 % functional condition evaluates collateral flow in extrahepatic portal hypertension

Irkutsk Region is one of the Russian Regions where botulism cases are registered yearly. Botulism morbidity in Irkutsk Region is analysed for the ten-year period (since 2002); its clinical-epidemiological features are revealed at the present stage. Sporadic cases of botulism, are dominated. (81,9 %). From. 2006 in Irkutsk Region botulism share makes 100 % in food poisoning number. A considerable role in the infection transmission plays consumption of fish, mainly smoked or salty Baikal omul (90,9 %). In 30 % of cases injured people purchased fish at private persons in unascertained trade places, 60 % of patients ate own salting fish. In Irkutsk Region botulism is registered more often from July till November with peaks in July (58,3 %) and. September (20,8 %) that is connected, with activation of unprofitable fabricated fish selling in combination, with adverse conditions of this foodstuff storage. Botulism affects various age groups of population with, prevalence of the age category 30-49 years old. (47,8 %). Socially active able-bodied population. (72,3 %) is most often affected that increases the social importance of the disease. Mainly, moderate severe (56, 5 %) and. severe (34,8 %) disease forms were registered. Primary symptoms include two variants - dyspeptically-paralytic and. ophthalmologic-neurological forms. Analysis of botulism etiological structure showed, that the leading part in the disease occurrence in Irkutsk Region belonged to botulinum. type Е (73, 9 %). Clinical material and. foodstuff testing to botulinum toxin presence was conducted in parallel by the standard reaction of biological toxin neutralisation in white mice and dot-immunoanalysis with application of the test system that we have developed. Possibility of dot-immunoassay application in laboratory diagnostics of botulism is estimated as the express method with high specificity, sensitivity and. simplicity.

The data of the study by continuous total disability due to affective disorders of the adult population in the Irkutsk region referral to the bureau of medical-social expertise in 2007—2011 years were presented. Intensive index was calculated per 1000 population (level). The percentage of primary disability for 5 years was 18,5 %, whereas redisability (81,5 %) prevailed. The level of total disability due to affective disorders has decreased from 0,16 in 2007, 0,18 in 2008 to 0,14 in the years of the period. People of working age were 82,5 % disabled due to affective disorders, while the retirement age - only 17,5 %. The mean total disability in working age (16,5) exceeded the rate of retirement age (11,5 per 1000). Women predominated, among the disabled. The level of total disability in men was more than two times lower.

The physical-chemical investigation on studying the influence of different concentrations of the barbituric acid. used in the photometric procedures on determining the cyanides, on the development and. stability of the potassium cyanide solution dyeing has been performed. The well-known methods on determining the cyanides in the air of the working zones, in the atmospheric air and in the drinking water were used as the material for studying. In these methods the photometric determination of the cyanides is based on the Kennig reaction. This reaction is also used to guantitative determining the rhodanides. The procedure analysis has shown that the researchers used the 6 % and. 3 % solutions of barbituric acid to perform the reaction for the quantitative measurement of the cyanides in different media. It was experimentally revealed that using the 3% solution of barbituric acid for the reaction, painting the potassium cyanide solution may reach the maximal value 5 minutes after the experiment start and. it was found to be stable for 40 minutes. Using the 6 % solution of barbituric acid, the potassium cyanide solution color was more found to be unstable. As a result of the experiments which were performed according to the methodical instructions on measuring the concentrations of the harmful substances in the air of the working zone, the concentration was chosen which would provide the optimal conditions for developing the color and. stability of the painted complex formed. The trueness of the results obtained was confirmed by the calculations of the average quadratic declination (S) and. the molar coefficient of the light absorption. (E). The spectroscopic character of potassium cyanide solution, painted was assessed. It was found that the optical density of the cyanide solution painted is recommended to measure at one of three wave lengths - 584, 586 and 588 nm in its photometric determination with the use of chloroamine, pyridine and barbituric acid. The other composition of the pyridine - barbituric reagent in which the 1 % barbituric acid solution is used was offered. This modification in the composition of the pyridine - barbituric reagent may provide the condition in which the colour of the final reaction product may be stable for 2 hours, which may exert the positive influence on both the quantitative measurements of cyanide in the sample and. in the reagent economy.

We investigated, toxicity and detoxification of model solutions of arsenic salts (Na₃AsO₄) by biotesting. Decreasing the toxicity of arsenic using humic substances («Powhumus», «Lignohumate», and «Humate-80») is shown. The possibility of use of humic substances to detoxify arsenic contamination, in model experiments is studied. To study the possibility of detoxification model contamination by salt of arsenic we used humic substances Powhumus (humate from leonardite «Humintech Ltd», Germany), Humate-80 (potassium, humate LLC «Agricultural Technology») and. Lignohumate (potassium, humate «SPA «RET»). As test objects seeds of Lepidium. sativum. L. (JSC «Irkutsk seeds») and. algae (Scenedesmus quadricauda (Turp.) Breb.) were taken. Toxicity was evaluated by effect on seed germination and. root length of L. sativum, the change in intensity of chlorophyll fluorescence of algae cells S. quadricauda. The significance of differences was determined, by Student's test. The table shows the mean values and. standard deviations for p > 0,95. Sodium arsenite at a concentration, of 8 mg/cdm inhibited seed germination of cress to 70,1 ± 6,9 %. The content of 9 mg/cdm. Na₃AsO₄ reduced, the number of germinated seeds to 75,1 ± 6,6 %. LC₅₀ for this method was equal 5,7 mg/cdm. In applying the HS in a concentration, of 0,2 g/cdm a decrease toxicity of sodium arsenite to 25,3 ± 2,7 % was observed. The most effective model in reducing the toxicity of arsenic contamination was observed at a concentration of Powhumus 1,0 g/dm³ - the number of germinated, seeds was 90,1 ± 8,7 %. The next stage was the bioassay using the registration reducing chlorophyll fluorescence of algae cells S. quadricauda. Value of LC₅₀ for this method was 1,5 mg/cdm. The concentration of sodium arsenite 1,8 and 1,5 mg/ cdm suppressed, levels of chlorophyll fluorescence by more than 30 % (the level of chlorophyll fluorescence was 26,4 ± 3,2 % and. 54,5 ± 6,1 % respectively). Powhumus in concentration of 0,05 g/cdm reduced toxicity of samples for 36,7 ± 3,9 % and. 31,8 ± 3,4 %, respectively. Bioassay method of changing the intensity of chlorophyll fluorescence of cells of algae S. quadricauda showed greater sensitivity and. speed of the response than the method of assessing the impact on seed, germination. and seedling root length of L. sativum.

The purpose of the investigation was development of screening visual and quantitative method of strain selection and potential consortium of normal human microflora (including one of probiotic orientation) for future creation of new synbiotic, symbiotic and acellular prophylactic and medicinal forms significant for therapy of endoceliac diseases. We used strains of yeast Candida and lactobacilli Lactobacillus, isolated from normal microflora of urogenital biotope of healthy donors. Suspensions of strains with turbidity 1 unit of Mcfarland standards were added with increasing doses to wells of polystyrene micropanels with surplus of MRS broth (variants A, B, C, D and E) and incubated 48 hours at 37 °C. Cultivated biofilms were stained with gentian violet and bottoms of wells in micropanels were photographed and scanned. Optical density of ethanol fixated stained biofilms in wells was registered with colour filter 620 nm. Visual evaluation was performed of influence of biofilm resolving activity and opposite action of polysaccharides and other adhesins. To assess ranging of expressiveness if biofilms growth in monocultures we suggested to use calculated coefficient (D + E)/(B + C), which reflects rectifying and decreasing of gradient of slope of dosage dependence of optical density in series of cultivations of microbial suspensions. The results make possible to perform monitoring of early film formation by strains of microorganisms and their mixtures. Micropanel is convenient for getting prognostic patterns of interference on film formation of co-existing in biotope consortiums of microorganisms populations and for evaluation of health status of biotope human microbiocenosises. It is possible to select strains biocompatible with human organism and their mixtures of probiotic orientation which are of great interest for prevention and therapy antibiofilm, antipathogenic, enzymatic, alternative to antibiotics, supporting adjuvant and combined

The article is devoted to the experimental study of changes of oxygen capacity and blood lactate concentration in conditions of multiple skeletal trauma. As the result of the research we revealed that indices that characterize oxygen capacity of blood of 22 Chinchilla rabbits in conditions of experimental multiple skeletal trauma didn't have significant changes during first 7 days after trauma. In first 3 days after trauma hyposiderosis was detected that is connected, in authors' opinion, with system inflammatory reaction and. is considered to be the reason of distinct tendency to anemia in a week after trauma. In laboratory animals during 7 days of observation after trauma significant decrease of concentration of lactate was determined. On the basis of obtained data authors concluded adequate delivery of oxygen to the tissues and. absence of circulatory hypoxia in laboratory animals of an experimental group at the realization of tolerant (passive) adaptation in conditions of multiple skeletal trauma

The article is devoted to the estimation of morphological changes in liver of experimental animals with acute hepatic failure caused by toxic injury of liver under the influence of xenotransplantation of culture of cryopreserved cells of liver and isolated cryopreserved neonatal hepatocytes. It was determined that undamaged hepatocytes with simultaneous decrease of number of vacuolated cells, preservation. of beam structure, decrease of fat insertions prevailed in the liver of animals with xenotransplantation of culture of cryopreserved cells of liver to the 6^th day. At the transplantation of isolated neonatal hepatocytes number of hepatocytes with undamaged structure decreased and number of damaged hepatocytes increased. Thus, cultivated cryopreserved cells of liver are the cells of choice of correction of acute toxic liver injury.

Performed studies showed an actual dosed aerogenic load of microorganisms-producers of a fodder-protein on the organisms of workers of two enterprises of microbiological industry. A quantitative value of air pollution levels of a working zone allowed to reveal the dependence of a production-induced morbidity, mico-carrying and a specific resistance of organism to a dosed load of microorganism-producers. The following levels of risk were identified: minimum when the actual dosed load does doesn't exceed the limits of the normative; increased when the actual dosed load exceeds the normative up to 6,5 times; high when the ratio of the excess is more than 6,5 times. The frequency of detection of criteria significant indices of prenosological diagnostics (combination of mico-carrying with positive results of allergotests) in the zone of minimal risk gradually grows and. is insignificant at the same time. While the dosed load is growing the number of working with a combination of these symptoms increases too. In the zone of high risk there is a sharp rise of frequency of detection of indices, that are the criteria of prenosological diagnostics of professional risk. The proposed criteria of risk of the development of vocational and production-induced pathology will allow occupational pathologists and. craft physicians to link the resulting violations in the health status of employees with an action of professional microbial factor even in the absence of the maximum allowable concentration of microorganisms-producers in the air of industrial premises. A safe level of exposure to microorganism-producers of a fodder protein cannon be recommended on this stage of the study, because even a minimal dosed load would lead to forming myco-carrying, specific alterations of an immune system and. higher levels of production-induced morbidity. While analyzing the results of the conducted. work we can conclude that the concept of limen concentration at the regulation of maximum allowable concentration for relatively-pathogenic microorganisms-producers needs to be clarified and. its possible adjustment and. dosed load of microorganisms-producers of the positions dose-spectacular dependencies requires more cautious approach to the evaluation for the enterprises of biotechnological synthesis.

The objective of the work was to study the distribution of metalloprotease 9 (MMP9) in tissues after myocardial infarction. We studied anatomopathological material from 30 patients, the primary victims of acute transmural myocardial infarction. Average age - 63,7 years. There were 18 men and 12 women. 16 patients died in the period up to 3 days, 14 patients - in a period from 3 days to 1 month. Samples were fixed in formalin and immunohistochemical staining for MMP9 was conducted. We used rabbit IgG monoclonal antibody to MMP9 (Epitomics, Clone 1D: EP1254, Cat. N 2551-1, Lot YG 113001P) in the working dilution of 1: 100 - 1: 250. At fatal outcomes that happened within a few hours after the onset of a heart attack a large number of MMP9 was detected in neutrophils in the blood vessels at near infarction zone. In the period from 1 to 2 hours MMP9 was detected in the extracellular matrix in the area of infarction. In the period from 3 to 30 days MMP9 was detected only in fibroclasts at forming cardiosclerosis zone. Thus, the use of staining for MMP9 is convenient for determination of the time elapsed after myocardial infarction in post mortem examination.

The natural plague foci of Kazakhstan occupy a huge territory. The mapping of these foci is a very important part of the plague monitoring and has a scientific and practical meaning. The old paper mapped data have been obsolete and. all previous and. current data have been digitized now. The digitizing of the data was carried by ArcGIS program. The epidemiological and epizootological analysis of the data of 2012 showed that the Kazakh plague natural foci were active in that time. And the analysis showed that by the complex factors a more active and epidemic hazardous area was Qyzylorda Region.

Emergency situations associated with infectious diseases dangerous for humans are actual for the Russian Federation. The probability of the occurrence of these events varies for different Russian Federation regions, but also for the individual municipalities of one region. Complex approach to the organization of anti-epidemic readiness for such situations is necessary. This approach should be based on differentiation of municipalities of a Russian Federation region according to the estimation of external and internal epidemiological risks associated. with diseases dangerous for humans. At the federal level the system of epidemiological surveillance is determined for diseases requiring measures for sanitary protection of the territory, and separately for almost every infectious disease, affecting the sanitary-epidemiological welfare of the population of the Russian Federation. In the frames of this study it is offered to consider the probability of the importation of infectious diseases, requiring measures for sanitary protection of the territory, as the external epidemiological risk. Diseases common to man and. animals, other endemic to the Russia infectious diseases, including relevant in regional pathology, are viewed as the potential and real internal epidemiological risk. The purpose of this study is the development of the control system of these epidemiological risks at the Municipal level. The three-level control system of the epidemiological risks according to differentiation of municipalities of a Russian Federation region by criteria of external and. internal epidemiological risks is proposed as a result of this work. The control system allows suggesting a priority orientation of organizational, preventive and anti-epidemic actions in municipalities and. promotes rational distribution of forces and means of Public Health Institutions in cases of emergency situations in the field of sanitary-epidemiological welfare associated with diseases dangerous for humans.

In the world the tendency of growth of cholera cases has been increasing. In Kazakhstan in 2011 epidemic cholera strains were isolated from the environment. In 2012 the cholera situation was quite stable. The cholera strains were isolated from the environment. Strains V. cholerae non O1 were isolated from people. But all isolated strains were not hazardous. By the complex of factors the South. Kazakhstan. Region is more cholera unfavorable region in Kazakhstan

The review presents modern data on the role of immune system in development of postoperative complications at hip replacement. Surgical stress while having depressive influence on immune system is powerful factor that causes conditions for development of secondary immune depression or intensifies it. On this background possibility of development of both early and late postoperative complications is quite high, that's why frequency of revision hip replacement increases steadily. Providing of stability of endoprosthesis is extremely important at total hip replacement. Researches realized during last decades determined connection of processes of osteogenesis and bone resorption and polypeptide factors of growth - cytokines. That's why questions of prevention and. correction of loss of bone substance with use of modern pharmacological preparations are important in the movement of observed problem. Infectious complications are also very significant ones at hip replacement. The fact of infectious process being the result of interrelation of macro- and. microorganisms and. immune system having one the leading roles in this process is doubtless. Among all the measures for prevention of postoperative complications their prevention that allows to determine right tactics of operative treatment, to realize complex of preparatory and. rehabilitation measures and. to improve the results of treatment and quality of life of patient, becomes important. Use of immunologic methods allowed to choose criteria and to develop ways of prevention of development of complications at hip replacement. However proposed ways not always allow to count individual susceptibility of an organism to different infectious pathogens; most of them are based on the determination of various specific and. quite laborious indices. Necessity of realization of researches before an operation and. in different terms of postoperative period for determination of dynamic dependence of changes of laboratory indices is significant disadvantage and requires quite prolonged observation of the patient for getting an information about prevention of hip replacement results. That's why further development and use of new ways of early hip replacement undoubtedly allows to improve results of surgical treatment of patients with different forms of coxarthrosis.

Tuberculosis is nowadays one of the most actual problems of world public health, service. According to the data of WHO, one third of world's population is infected, with tuberculosis microbacteria. Every year 2-3 millions of people die because of tuberculosis. During last years in Russia tuberculosis morbidity increased 2,3 times, mortality - 1,9 times and prevalence - 4 %. Aggravation of epidemiological situation on tuberculosis is characterized by increase of morbidity among all age groups and especially among the children. In Irkutsk region epidemiological situation on tuberculosis is still intensive. Main reasons of unsatisfactory indices of tuberculosis in Irkutsk region are: presence of significant reservoir of tuberculosis infection among the population. of the region; significant level of prevalence of HIV-infection; unsatisfactory material and technical base of antituberculous institutions of the region; personnel deficiency in antituberculous institutions; insufficient level of preventive measures in foci of tuberculosis infection; absence of modern, medical informational system in antituberculous institutions of the region. To optimize the situation at the development of antituberculous measures it is necessary to take into account regional peculiarities of epidemic situation, to realize control and. organization of an aid. for children from the foci of tuberculosis infection and. to carry out controlled chemical prophylaxis in sanatorium conditions.

This review presents the observation of the role of endothelial dysfunction in development of atherosclerosis, coronary disease and. hypertension. Among other causes of endothelial dysfunction the most significant are hemodynamic factors (wall transverse strain, transmural pressure), dyslipoproteinemia, hyperhomocysteinemia, hyperglycemia, free radical damage of endothelium. It is supposed that in development and. progressing of coronary disease more significant role may belong to endothelial dysfunction rather than to morphological vascular changes, predetermined by atherosclerosis. However, there is still no clear view of development of endothelial dysfunction and. mechanisms of development and. progressing of both isolated forms of coronary disease and arterial hypertension and their combination.

This work deals with the literature review on the problems associated with the progress of the national and foreign authors in studying the role of the cytokine net in the pathogenetic mechanisms of forming the diseases of the nervous system. Studying the peculiarities in the functions of a number of the immune system indices as the targets of the combined exposure to the production and other factors are known to be the actual aspect of the works analyzed in the recent years. A special attention in the literature studied is paid to the intersystem interactions between the nervous and the immune systems and. the immune systems and. the value of their homeostasis. The literature deals with the causes of the injuries and/or the cellular death of the nervous system and, as the consequence, the function changes of other organism systems and the production of their activity products. The change regularities in the pro-inflammatory cytokines (IL-1β, TNF-α, IL-6 and IL-12) in the diseases of different etiology including those which were stipulated by the factors of the production environment and other factors on the example of the multiple sclerosis as the neurodegenerative disease are represented in this paper. The possibilities of the disease objectify of the nervous system and the determination of their process severity in the clinical praxis based on the immunological indices and the patient complaints are given in this work. The studies testifying the different mechanisms of the autoimmune response realization in exposure to the neuro toxicant of different etiology are represented in this paper as well as the direct correlation between the antibody content to the proteins of the nervous tissue and the production of the pro-inflammatory cytokines has been revealed. At the same time, the common morpho-functional regularities of the changes in the central nervous system of the albino rats in the post-contact period as a result of the exposure to the metallic mercury vapors and. sublimate including the persons examined working at the production were indicated. In the conclusion. the important actuality role of the molecular-genetic studies dealing with the gene polymorphism of the cytokine net in the ethiopathogenesis of the autoimmune disease and. the problems of the early diagnostics of the neurodegenerative disorders in the employees exposed to the neurotoxicants of different chemical nature is indicated.