No 5(1) (2012)
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ПЕРЕДОВАЯ СТАТЬЯ
13-21 521
Abstract
This paper is about 100 years history of the Institute of the epidemiology and microbiology (Irkutsk), development of scientific researches and practical help to the public health.
CLINICAL MEDICINE
22-24 651
Abstract
Lung resection specimens of 677 surgical patients were studied to determine the clinical meaning of the absence of Mycobacterium tuberculosis (MTB) in sputums of pulmonary tuberculosis patients, who show none of the clinical manifestations of tuberculosis, except the alterations seen on x-ray pictures. There were 2 patient groups: group 1 comprised. 409 patients referred to a sputum-positive out-patient follow-up group, group 2 included 268 patients referred to a sputum-negative out-patient follow-up group, who showed the presence of mycobacteria in their lung resection specimens by luminescence microscopy in 147 (54,8 %) cases, and of them. 58 (39,4 %) cases showed, high counts of mycobacteria. By culture method. MTB were identified in 35 (13,0 %) patients, and. in 14 (40,0 %) cases growth was either medium or massive. The question was studied of how should one treat this fact: carrier state or latent tuberculosis? We conclude that patients with the absence of bacterial expectoration confirmation by laboratory methods and. the absence of disease manifestations, but who have definite morphologic signs of tuberculous alterations in the lungs, should be treated like epidemiologically risky group and. should be appointed to radical surgical treatment methods.
25-28 477
Abstract
The purpose of a study was the prevalence of chronic heart failure under the dependence on the sex and. the age based on the example of the representative sample of adult population of Irkutsk. The prevalence of chronic heart insufficiency composed. 9,7 %.
29-31 460
Abstract
Epidemiological study of allergic rhinitis symptoms frequency was undertaken on ISAAC program in children 7-8 years and 13-14 years in Irkutsk city in 1998-1999 and 2008-2009. According to the results of the study, the frequency of symptoms of rhinoconjunctivitis among schoolchildren, has increased during 10 years period. The high degree of combination of asthma and rhinitis was observed.
32-34 484
Abstract
It is paid a lot of attention to the problem of mycobacteria tuberculosis sensitivity to antituberculosis drugs. The main problem is in multidrug-resistance. Micobacteria tuberculosis sensitivity to antituberculosis drugs was monitored, tuberculosis patients were observed during 2008-2010 years. Drug sensitivity of 2251 strain of mycobacteria tuberculosis that were isolated from just evaluated and previously treated patients was studied using the method of absolute concentration. There is an upward trend in total frequency of Mycobacterium tuberculosis resistance. The reason is the increasing of the multidrug-resistant strains detection rate.
35-37 424
Abstract
We retrospectively analyzed. 890 medical histories of patients age from 1 days to 90 years. 225 of them were children and 665 were adult. AH of patients were admitted, to ICU of different hospitals in Irkutsk region. Hypovolemia was diagnosed in 75 % (496/665) of adult patients and in 81 % (183/225) of children. We found that the mortality of the adult patients depends on not only degree of hypovolemia but also on its duration.
38-40 529
Abstract
The purpose of a research was the study of the frequency of arthralgia, prevalence of the diseases of osteomuscular system, and fractures among the persons from 14 to 24 years in Irkutsk. The frequency of arthralgia composed. 22,4 %. The prevalence of the diseases of osteomuscular system composed. 36561 to 100000. Fractures occurred in 23,8 % people at the age of up to 24 years.
41-43 416
Abstract
The specific features of immune dysfunction in HIV-infected persons were studied, taking into account the stage of disease, the route of infection and climatic conditions of residence. Three hundred and. twenty-two HIV-infected patients aged from 17 to 57 years were examined. Of those with sexual transmission were 185 (Group II), with the parenteral route of transmission - 137 (Group I). The findings suggest that there are pronounced and. significant cellular and. humoral changes: decreases in the level of CD4-!ymphocytes, in the immunoregulatory index (IRI), B-lymphocytes and. increased the content of CD3, CD8-lymphocytes, a rise in the concentration of IgG, IgM, IgE, and. increased formation of circulating immune complexes (CIC). These changes were observed in all subjects, but most pronounced in the group with the parenteral route of transmission, as well as in stage 4 A-B HIV infection. The degree of damage the immune system is more pronounced in comparison with the data obtained in other territories.
44-46 498
Abstract
Periodontitis the same as brain, heart, kidneys is an organ-target for an arterial hypertensia. Disturbances in system of the microcirculatory bed, influencing a trophism of tissues of dentoalveolar system that promotes more active course of pathological processes in periodontitis thus develop. Actual revealing periodontitis pathogenic microorganisms in patients with an essential arterial hypertensia thereupon is represented. By us it is spent indication of five periodontitis pathogenic microorganisms, which most often causing development of diseases, in teenagers with an essential hypertensia by means of test system «Multident-5» («Genlab», Moscow).
47-50 401
Abstract
A total 61 randomly selected strains of M. tuberculosis were genotyped by the 12 loci of MIRU-VNTR. It was shown the predominance of the Beijing genetic group (78,4 %) in population of mycobacterium from the patients with tuberculosis and. human immunodeficiency virus infection in Irkutsk region and. Buryatia. Multidrug resistance was observed in 34,4 % of the studied strains without significant differences among representatives of Beijing and non-Beijing.
51-53 420
Abstract
The results of the clinical examination and supervision of 62 patients under average-severe course of coxiellosis at the age of 17-74: the first group (32 persons) - patients younger than 50, the second group (30 persons) -patients older than 50 are presented here. Catalase and ceruloplasmin have been defined in the blood serum of the patients with coxiellosis in the disease dynamics. Clinical evidences of coxiellosis: hyperaemia of the pharynx, shivering, myalgy, weakness, scleroconjunctivitis and. arthralgy have been seen longer in the patients older than 50. Catalase and. ceruloplasmin. content depended on the period of the disease and the patients age. At age first week of the disease there noted decreasing of the сatalase levels in 80 % of the patients younger than 50; but in 100 % of the patients older than 50 increasing was noted. At the second week of the disease most of the patients, regardless of their age, had. decreased the levels of catalase. Increase of the levels of ceruloplasmine has been noted the elder aged group. At the third week of the disease at the background of ceasing of clinical evidences of the disease most patients with coxiellosis, independent on age, had. increased content of the studied indices, which shows the compensatory activation of the antioxidant protection.
54-55 465
Abstract
The results of clinical follow-up and examination of 41 patients with average-severe course of coxiellosis at the age of 17-48 years (39 males and 2 females) are presented in this study. Hypersensitivity of the slow-type reaction of stopping of migration of leucocytes with pulmonary extract has been discovered in the patients with coxiellosis in dynamics and. in 30 donors. 29,3 % patients complained on tightness in the chest and. cough. Auscultation revealed hard breathing in 65,9 % patients with coxiellosis, dry and. moist rattles in 19,5 % patients. X-ray examinations confirmed the presence of bronchitis in 9,8 % patients and. pneumonia - 2,4 %. Formation of hypersensitivity of the slow-type depended on the period of the disease. Stopping of migration of leucocytes has seen noted at the first, second, and third weeks of the disease in 25,0, 35,3 and. 50,0 % patients, which shows the formation of the cellular autosensibilisation the pulmonary tissue under coxiellosis.
56-58 447
Abstract
This study of multidrug resistance (MDR) of tuberculosis mycobacterium. (TMB) frequency has revealed, that in 2011 28,4 % persons discharging bacteria with, newly diagnosed pulmonary tuberculosis had multidrug resistance of tuberculosis mycobacterium and among them are (70,1 %), rural population. (57,7 %), non-workers of employable age (56,7 %) prevailed. Among patients with, multidrug resistance (MDR) of tuberculosis mycobacterium. (TMB) there was a great propotion of patients with, disseminated tuberculosis (39,2 %), infiltrative tuberculosis (44,3 %), caseous pneumonia (7,2 %); there were 55,6 % of cases with profuse bacterioexcretin due to the violation of the Chest X-ray examination, terms. 45,3 % of patients with multidrug resistance also had. resistance to other essential antituberculosis drugs; in 54,7 % cases besides combination of this resistance there was also the rescue therapy drug resistance. Medical comment was made that under modern conditions it is not advisable to use chemotherapy of the first mode with patients with newly diagnosed pulmonary tuberculosis.
MYCOLOGICAL STUDYING DURING VARIOUS DISEASES OF GASTROINTESTINAL, RESPIRATORY AND UROGENITAL SYSTEMS
59-62 427
Abstract
The results of mycological studies of variety of biosamples during gastrointestinal, respiratory and. genitourinary diseases in different patients we analyzed during the period from 2006 to 2010 years. Different types of fungi were found at gastritis (15-30 % of cases), gastric cancers (5-30 %), duodenal ulcer (3-12 % ), diseases of the respiratory system. (9-14 %). In the cases of prophylactic gynecological evaluations the fungi were found in 12-15 % of women; among these 19-28 % were healthy non-pregnant women and. 14-32 % healthy pregnant women.
63-67 419
Abstract
This paper presents results, which were obtained during investigation of 106 children with acute intestinal infections in order to determine the presence of cryptosporidia and to evaluate their intestinal microbial status. The cryptosporidia's oocysts were found in 31 of children (29,2 %) that were mainly under the 3 age old. At the same time the authors found the dysbacteriosis of the second and. third, degrees in 92,5 % of patients. Six patients (5,7 %) became cryptosporidium negative and improved their intestinal microbial status. The obtained results showed that lactobacterias containing probiotics are effective in treatment of children with acute intestinal infections for normalizing of intestinal biocenosis and. to eradicate cryptosporidia.
L. V. Lyalina,
M. E. Ignatyeva,
O. V. Narvskaya,
Yu. N. Filippova,
A. A. Vyazovaya,
E. V. Kasatkin,
E. V. Katkyavichene
68-71 484
Abstract
Results of the study in three regions of the Russian Federation have shown widespread of high carcinogenic human papillomavirus among patients of dermatovenerological and gynecological profile. Detection of viral DNA in the material from the cervix and urethra ranged from 25,2 (Karelia) to 42,5 (Sakha Republic (Yakutia) per 100 examined patients. In all areas in 2010-2011 first place was occupied by 16-th virus genotype - from 11,5 (Sakha Republic (Yakutia) to 15,9 (St. Petersburg) per 100 patients. Prevalence of 11 other types differs. In the Sakha Republic (Yakutia) the second rank place was occupied by types 31 and. 51 (8,0 per 100 examined patients), in St. Petersburg - by 56 and 31 types (9,7 and. 7,6 per 100 patients). Age risk group contains patients of the age of 20-29 years. Information on circulating genotypes of the virus is a necessary part of surveillance to validate vaccination against human papillomavirus infection and evaluation of its efficiency.
72-75 675
Abstract
Currently, Clostridium difficile is the main reason of a nosocomial diarrhea, caused by uncontrolled antibacterial treatment. This problem is not paid, enough attention in our country. We analyzed. 536 cases of antibiotic associated infections using new immunochromotographical assay for express detection of Clostridium difficile. Since 2008 to 2011 evaluated rate of the positive tests was 28,7 % among the hospital patients. The first line therapy of this infection is vancomycine and metronidazole. We also observed increased incidence of mycosis, which accompanying the antibiotic associated diarrheas. During the same period the rate of Candida spp. infection was 50,8 % among the same patients. We used fluconazole and. amphotericine for the mycosis treatment. We also recommended to manage disbiosis during one year after discontinue of the treatment, and. we supposed reasonable to be managed by infectionist for this group of patients.
76-78 453
Abstract
This paper presents data about the prevalence of fungal infections of the skin in Nizhneudinsk and Nizhneudinsk area for the period from 2006 to 2010 years. The nail fungal infections were recorded primarily in adults, with more males (38,1-67,4 %) than women (26,6- 33,5 % ). Fungal infections of the scalp were most often detected in children (41,8-64,8 %). Dermatophytosis of smooth, skin among women was met in 30-51,5 % of cases and. among the men it was 31,3-38,8 %. Since 2007 the disease was diagnosed increasinly in children (30,4-37,8 %) in connection with rout in medical examinations in remote locations of Nizhneudin.sk under the Program. «Children of the North».
79-83 435
Abstract
The information about epidemiology of osteoporotic injuries of vertebras among people of elderly and senile age of Irkutsk is provided in the article. It is shown that frequency of these injuries is 28 % (33,9 % in women and. 22,2 % in men), and. osteoporotic fractures of vertebras were revealed, at 23,6 % (at 28,9 % of women and. at 18,3 % of men) semi-quantitative and. at 22,8 % (28,3 % and. 17,2 % respectively) quantitative by radiological methods of an assessment of deformations of vertebras.
PREVENTIVE MEDICINE
84-85 426
Abstract
The use of the physical culture methods in the children's pre-school establishment promote health status improving in children with tuberculosis intoxication, normalizes the psycho-emotional condition of the children, promote of the basis formation of the healthy lifestyle.
86-88 427
Abstract
We performed graphic and statistical analysis of twelve-year data concerning the incidence of infections transmitted. by Ixodes ticks, their abundance and activity in the south of Primorye territory. We found significant correlation between the incidence and. level of vectors activity, as well as inverse relationships between activity of ticks and. tick-borne encephalitis incidence and. Lyme disease.
89-91 416
Abstract
The paper presents the results of health status study in pediatric population of the Transbaikal region. It was shown the prevalence of some forms of chronic diseases in dependence on age and residence. In addition, we revealed factors influencing to pathology development.
92-94 621
Abstract
Not only the growth of the death, rate and the morbidity rate can be considered as epidemiological manifestation. of the tuberculosis pathomorfism in modern conditions but also the changes of biological properties of the activator. Since 2003 till 2011 mycobacterium drug resistance with the patients with the newly diagnosed infiltrative tuberculosis which is of the most frequent occurrence has increased 1,4 times. The mycobacterium drug resistance has undergone the apparent qualitative changes. The percent of multidrug resistance has also increased. The combination of multidrug resistance and rescue therapy drug resistance has become more often. At the same time bacterioexcretin has become more profuse, mycobacterium growth rate has become slower and. the fact proves there is an interaction between two their biological properties - drug resistance and. growing power.
95-96 447
Abstract
We present the results of assessment of mortality key elements and trends concerning respiratory diseases in the Irkutsk region in comparison with Siberian District and whole Russian Federation during 1992-2009 years. It was found that the bronchial-pulmonary pathology is ranked first in the pattern of mortality from cancer and. infectious diseases. Also it was established that in recent years morbidity and mortality from pneumonia of the population of the Irkutsk region increased.
102-105 442
Abstract
Six hundred and ninety three cases of ixodid tick-borne borreliosis in Irkutsk city residents duing17-year period. (1995-2011) have been analyzed. It was revealed that the most risk of Borrelia burgdorferi infection is for visitors of zones along Baikalsky, Goloustnensky and. Melnichny routes as well as green zones in Irkutsk from the end. of May up to the beginning of July. Individuals older than 40 years of both sexes suffer from the disease more often. Erythema is marked in more than 80 % of patients. Body is character place of the tick's bite for adults, head - in children.
R. S. Musagaliyeva,
B. B. Atshabar,
U. A. Sagymbek,
A. Zh. Zholshorinov,
Z. B. Zhumadilova,
Z. A. Sagiyev,
A. K. Kasenova,
A. O. Ismailova,
G. S. Zhunusova,
A. S. Zholdasbekov,
R. S. Sailaubek,
K. R. Rahimov,
L. M. Atovulayeva,
D. S. Aliyev,
Zh. B. Medetov
106-108 403
Abstract
The analysis of registered cholera cases that occurred during 1993-2008 showed that all epidemic cases of cholera in Kazakhstan were imported. The toxigenic cholera microbes were isolated from the water reservoirs which started in the neighbor country. The spatial distribution of non-toxic cholera vibrio has the regional features. The climatic and anthropogenic factors affect the distribution of the cholera vibrio in the Kazakhstan. The results of the analysis show that the complex approach has to be used for cholera epidemiological monitoring.
N. V. Rudakov,
S. N. Shpynov,
V. K. Yastrebov,
I. E. Samoylenko,
L. V. Kumpan,
T. A. Reshetnikova,
N. V. Abramova,
A. N. Kolomeyetz
109-113 417
Abstract
The paper presents analysis of the development and the current status of the doctrine about rickettsia and rickettsiosis. Progress in the study of Rickettsia is associated with, improved methods for their detection and isolation - using live biotechnological systems (cultivation in the experimental lines of ticks-sensitive lines of eukaryotic cells) in combination with methods of genotyping and. classical ricketsial methods. The development and. use of new methodological approaches to the study of tick-borne rickettsiae and other alpha-proteobacteria led to an important revision of ideas about genetic diversity, heterogeneity of antigenic and. biological characteristics, ecology and. contribution to the infectious pathology of various representatives of the order Rickettsiales in Russia.
114-118 638
Abstract
Nowadays the evolution of epidemic process is under active influence of a new adjusting risk factor - technogenic environmental contamination. Negative influence of ecological pressure on manifestations of infectious diseases is realized in destabilization of epidemic process: significantly higher level of incidence, greater amplitude of its fluctuations around a trendline and. shortening cyclic componenta. Besides this, the more heavy clinical manifestations of infectious process and its longer duration with simultaneous development of the complications, accompanying diseases and. synchronization of process takes place. Decrease of immunological and epidemiological effectiveness of vaccination is also registered. This paper establishes, that the minimal period of infection incidence is the most vulnerable part in a uniform circuit of epidemic process. It is shown in epidemiological experiment that the complex of preventive actions at management of infection incidence is necessary for making active during a minimum level of development of epidemic process in its intraannual dynamics.
119-121 442
Abstract
Contrary tuberculosis epidemiology in the Irkutsk region is observed. There are a lot of factors underlain of this situation. Because of the Irkutsk region comprises a large amount of industrial sectors, we cannot ignore the role of anthropogenic impact. The aim. of the research was to study how epidemic process of pulmonary tuberculosis could manifest in conditions of technogenic pollution in large industrial cities in the Eastern Siberia. It was found that air pollution strongly effects to the formation of unfavorable respiratory tuberculosis epidemiology.
I. A. Sobolev,
O. G. Kurskaya,
E. V. Ivanova,
A. G. Durymanov,
T. N. Ilyicheva,
V. N. Mickheyev,
A. M. Shestopalov
122-126 401
Abstract
In the result of the monitoring for influenza in the Novosibirsk region during the season 2011-2012 85 strains of influenza virus on the culture of MDCK cells were isolated: 79 strains A (H3N2) and 6 strains of influenza virus B (five strains belonged to the Yamagata line and. 1 strain belonged to the Victoria line). During the entire epidemic season 2011-2012 on the territory of the Novosibirsk region no strains of influenza A (H1N1) pdm09 were allocated. For molecular genetic analysis 3 strains of influenza A (H3N2) were selected. We determined full nucleotide sequence of NA and. HA genes encoding respective surface glycoproteins of influenza virus, as well as the comparative analysis of amino acid sequences of these proteins to the strains of influenza A (H3N2), vaccine strain and. the strain A/Victoria/361/2011 earlier seasons, for which diagnostic serum were obtained.
127-131 518
Abstract
The paper presents the data of complex epidemiological analysis of infectious diseases from fecal-oral transmission. in the Republic of Sakha (Yakutia) from 2002 to 2011. We found increased levels of acute intestinal infections of unknown etiology, and. set against a background of reducing the incidence of dysentery. It was found that in the Republic of Sakha (Yakutia) the epidemiological situation is characterized by greater intensity in comparison with the Russian Federation. We identified area of risk for the incidence of acute intestinal infections and. dysentery in adult and. child population of the Republic of Sakha (Yakutia).
132-134 387
Abstract
The analysis of morbidity of the highly pathogenic influenza and acute respiratory viral infections in Republic of Buryatiya is lead. Data of laboratory monitoring behind circulation of pathogen agent of flue and the immunization of the population in preepidemic period are presented. Organizational and. practical actions on counteraction to flu A/H1N1/ are consecrated.
135-136 428
Abstract
The analysis of long-term morbidity of acute and chronic viral hepatitis B in the Republic of Buryatia is lead. The data of inspections of HBV infection among the population of republic are presented. The interrelation between morbidity and. immunization of the population against a virus of hepatitis B is revealed. The results of the realization priority national project of healthcare on additional immunization of the population against HBV infection in republic are given.
137-140 436
Abstract
In this paper, based on a retrospective analysis of hepatitis A, shigellosis Flexner and Sonne total population of the various administrative areas of Primorye Territory in the period. 1995-1999 and. 2004-2011 years and. conducted studies of the correlation between the incidence and. the proportion of these infections in the demographic structure of the population, areas of the region up to 14 children and. up to 6 years attending preschool institutions, given the epidemiological assessment of the impact of this factor on the spatial dimension of the epidemic process under studied nozoform was studied.
141-144 478
Abstract
Pattern of age, sex and social composition of newly diagnosed patients with infiltrative pulmonary tuberculosis was studied as well as characteristic features of the developed particular process and its clinical manifestations in three phases, covering a 20-year period, of observation (1989 - 105 patients, 2004 - 161 patients, 2009 - 399 patients). It was found that for 20 years infiltrative tuberculosis was subjected to significant pathomorphism which showed an increase in the proportion of cases among persons of older age groups and. women, an increasing role of exogenous superinfection and. concomitant diseases of the risk group in its pathogenesis, growth of damage at a constant rate of destruction and. bacterioexcretion. It is stated that in the dynamics there is an increase in frequency of primary drug resistance in tuberculosis Mycobacterium and. the loading of its structure. The features of clinical and. laboratory manifestations of infiltrative pulmonary tuberculosis are distinguished in modern conditions.
145-150 457
Abstract
In the article using the binomial distribution analysis of the intensity of рориlation antitoxic (AT) and antibacterial (AB) of immunity to diphtheria in terms of specific diphtheria antibodies in the sera of healthy adults aged 20 to 51 years and. older, living in Russia and. Italy. The latter, in contrast to Russia, during the epidemic was only a few cases of diphtheria. For the population of people from Italy the optimal parameters of tension antibody immunity (bimodal distribution of the concentrations of AT antibodies) are determined, whereas for the adult population of Russia experienced an abnormally high, overvoltages antibody immunity (lack of bimodal distribution). The level of concentration of antibody isotypes AB M, G, A in both countries match the total synthesis of immunoglobulin isotypes in the ontogeny of these: thus, in the sera of adults aged 20 to 40 years AB antibody IgG prevail. Low concentrations of antitoxin in the blood of most adults living in Italy, recharged high AB IgG in these same serum, and. optimal concentrations of AB IgM and. IgA. This indicates the possibility lifting the mass revaccination of adults, and. creating an individual approach to their boosters. Optimal bimodal distribution of AB antibodies in the population of healthy adults, both in Russia and. in Italy, is an additional protection to the diphtheria antitoxic resistance.
151-154 528
Abstract
The analysis of the incidence of diphtheria in the Russian Federation after the second revaccination of adults since 2005 to 2011 is presented. The level of the immunization and. the state of population immunity were studied. The high level of protection against diphtheria was established. It provides positive epidemiological situation against this infection in the country.
EXPERIMENTAL RESEARCHES IN BIOLOGY AND MEDICINE
T. .. Masuzawa,
N. .. Ohashi,
M. A. Khasnatinov,
G. A. Danchinova,
L. I. Ivanov,
T. .. Fukui,
Y. .. Okamoto,
M. J. Pan,
N. .. Koizumi
155-157 350
Abstract
To determine the reservoir animals and vector ticks for Anaplasma phagocytophilum in Far East Asia, which causes human granulocytic anaplasmosis, we analyzed tissue samples from deer and boars in Japan rodents in Taiwan and. Ixodes persulcatus in Russia by PCR-targeted. to 16S rDNA. Anaplasma species including Anaplasma bovis and. Anaplasma centrale-infected wild deer and boars were detected. The detection rates for A. phagocytophilum, A. bovis and. A. centrale in deer were 15,6 %, 21,9 % and. 37,5 %, respectively. These infection rates in wild boar were 3,6 %, 17,9 % and. 3,6 %, respectively. Wild rodents captured in Taiwan were positive for A. phagocytophilum. and. A. bovis. Prevalence rate of A. phagocytophilum. on I. persulcatus ticks in Irkutsk and. in Khabarovsk were 6,3 % and. 11,3 %, respectively. The 16SrDNA sequences detected from Russian ticks were identical to those of A. phagocytophilum. detected, in US and. Europe, and. from tick Ixodes ovatus and. Ixodes persulcatus in Japan. However the sequence detected from deer and boars in Japan were identical to sequences previously detected from deer and cattle in Japan, and. showed. less similarity (98,6 %) with typical A. phagocytophilum.. Sequences detected from wild rodents collected in Taiwan showed higher similarity (99,7 %) with typical A. phagocytophilum but formed the branch from those of A. phagocytophilum. detected in US and Europe. The finding suggests that the A. phagocytophilum-related sequence detected from deer and boars in Japan, and. wild rodents in Taiwan were different from those of typical A. phagocytophilum. found in Ixodid ticks.
158-162 399
Abstract
Segregation of hantaviruses into clades that parallel the molecular phylogeny of rodents (order Rodentia) in the Murinae, Arvicolinae, Neotominae and Sigmodontinae subfamilies suggests that hantaviruses may have co-evolved with their reservoir hosts. Recently, however, host switching and local species-specific adaptation have been proposed, to account for the similarities between host and. virus phylogenies. The demonstration, that Thottapalayam. virus, a hantavirus isolated, from the Asian house shrew (Suncus murinus), represents an early evolutionary divergence from other hantaviruses has spurred, a search, to ascertain if small mammals having shared, ecosystems with, rodents, such, as shrews (order Soricomorpha, family Soricidae) and. moles (family Tal-pidae), as well as bats (order Chiroptera), may have figured prominently in the evolution and. diversification of hantaviruses. Archival tissues from 1,258 shrews (47 species), 152 moles (10 species) and. 329 bats (32 species), captured, in Europe, Asia, Africa and. North. America in 1980-2012, were analyzed, for hantavirus RNA by RT-PCR. The realization, that newfound, hantaviruses detected, in soricomorphs are genetically more diverse than those harbored, by rodents suggests that the evolutionary history of hantaviruses is more ancient and. complex than previously conjectured and. that ancestral rodents may not have been the original mammalian hosts of primordial hantaviruses.
163-165 407
Abstract
Changes in lung, liver and brain were investigated, in wild, ducks and. domestic chicken under experimental infection caused by highly pathogenic avian Influenza virus A/H5N1. Isolation of virus was demonstrated, in swabs from the throat and. cloaca, virus titers were presented, in patterns of organs. Changes in organs of wild, ducks have dystrophic characteristics, but in domestic chickens are of the dystrophic and necrotic nature.
E. Yu. Abiduyeva,
L. P. Kozyreva,
D. D. Tsyrenova,
B. B. Namsarayev,
B. B. Batobolotova,
E. B. Matyugina
166-170 438
Abstract
On microbiological and sanitary-bacteriological parameters investigated, include the Zabaikal region lakes to clean, mildly and. moderately polluted, waters. However, in the summer antropogenic pressures on the aquatic ecosystem, of some lakes increases, which, leads to deterioration, of water quality.
D. .. Abmed,
Zh. .. Bataa,
D. .. Anu,
M. A. Khasnatinov,
G. A. Danchinova,
P. .. Nimidavaa,
D. .. Nimahuu
171-172 428
Abstract
During 1999-2011 we conducted the collaborative research in Northern, Central and Eastern Mongolia. Several tick-borne infections were revealed in ticks and. human patients. Most important of them are tick-borne encephalitis, Lyme disease, tick-borne rickettsiosis, human granulocytic anaplasmosis and. babesiosis. The importance, risk of infection and. spread of tick-borne diseases in Mongolia are discussed in the paper.
173-176 440
Abstract
The influence of 2-hydroxyethylammonium salts of biologically active arylsulfanylacetic acids on the yield of bacterial mass of Staphylococcus aureus (strain 209), biomass of Merezhkovsky's bacteria (Salmonella typhi spermophilorum.) and E. coli (E. coli М-17) has bee studied for the first time. The introduction of growth stimulators (in concentration of 10-3-10-8 wt. %) into nutrient medium increases the yield of microorganisms bacterial mass: Staphilococcus aureus by 32-40 %, Merezhkovsky's bacteria Salmonella typhi spermophilorum. by 47,5-140,4 %, E. coli by 11,4-59,4 %. The increase of yield of the microorganisms biomass reached by application of the growth stimulators opens the route to their utilization in large-scale processes of Staphilococcus aureus cultivation for the production of valuable protein А, biopreparations, diagnostic agents, serum, vaccines, probiotic drugs as well as for the struggle with rodents (plaque carriers, pests).
177-183 433
Abstract
On the basis of studying of features of structure of 7 genes determining major factors of pathogenicity of the causative agent of whooping cough - ptxA, ptxB, ptxC, ptxD, ptxE, ptxP and prn, features of genotyping variability of strains of B.pertussis allocated from patients with whooping cough in Russia are shown. Dynamics of formation of population of strains of the causative agent of whooping cough is tracked and is established that population of strains of B.pertussis is formed by clonal expansion of strains with, new genetic structure of major factors of pathogenicity - pertussis toxin and. pertactin. The structure of population of strains of B.pertussis causing a disease by whooping cough, at the present stage of epidemic process of a pertussis infection - domination. of strains with new «non-vaccine» alleles genes - ptxA1 (97,7 %), ptxC2 (87,4 %), prn2 (89,5 %) and. ptxP3 (93,3 %), in 2,2 % cases with ptxB2 allele and. in 1,3 % cases with. prn9 allele circulate.
184-186 510
Abstract
The results of the study of opportunistic bacteria of the Lena river are show in the article. The microbial community of the river is represented by bacteria of different families. Opportunistic Enterobacteriaceae is absolutely dominated. Enterobacteria of different type belonged to four classes of dominance. The fluctuations in the frequency of their occurrence did. not extend beyond the class for the period of observation. At the same time the changes in their populations at the level of diversity are identified.
N. M. Andreevskaya,
V. A. Mikhailova,
E. S. Karetnikova,
A. G. Atlas,
L. M. Mikhailov,
A. I. Kalinovsky,
N. G. Gefan,
N. L. Barannikova
187-189 448
Abstract
At present no commercial polyvalent brucellosis serum is produced in Russia. We developed a technology of polyvalent brucellosis high-effective and specific serum production without adsorption. Experimental-production series are obeyed the technical specification project.
190-192 416
Abstract
Data of the experimental investigation, using typical for the focus virulent Yersinia pestis subsp. pestis strains were analyzed to detect special features of a plague microbe biofilm formation (conglomerates, «blocks») in the main plague carrier, Citellophilus tesquorum altaicus fleas, in Tuva natural focus. It was established that biofilm (bacterial «blocks») played the important role both in plague transmission, by fleas and also in the agent preservation, by these insects. Results of the analysis indicated the prevailing significance of C. tesquorum. altaicus females in plague agent preservation and. explained some mechanisms of its survival in fleas during a cold season in Tuva natural plague focus. Under adverse conditions during the autumn period Y. pestis biofilms («blocks») accumulate mainly in a digestive tract of the main carrier females and. so can survive in fleas for a long time.
193-196 466
Abstract
Studying of distribution and number of indicator microorganisms has allowed to estimate springs water quality by microbiological indicators. The koliformal bacteria have been found in water and. ground precipitation of hot and. cold springs. Opportunistic microorganisms have been allocated - Enterobacter aewgenes, Klebsiella oxytoka, Escherichia coli, Enterobacter agglomerans, Enterobacter cloacae, Enterobacter amnigenus (biogroup 2), Citrobacter Freundi.
197-200 992
Abstract
The article presents the results of determination of residual ethanol in the preparation of heterologous antirabies immunoglobulin, using portable photoionization gas chromatograph PGC-1. The advantages of the method are as follows: accuracy, sensitivity, promptness. It does not require sample preparation; the results can be processed automatically.
201-205 441
Abstract
The technology of isolation of the cholera vibrio complex of low-molecular enzymes related to pathogenicity factors, from cholera vaccine production wastes by means of concentrating ultrafiltration was developed. This technology allows to obtain standard series of the preparation termed proteovibrin which is free from the basic Vibrio cholerae immunogen - O-antigen, and possesses high activity of protease and. phospholipases A2 and. C. It is shown that proteovibrin is highly soluble in water and 0.14 molar NaCl solution, causes the production of antibodies in rabbits and is toxic for white mice. Its further examination seems to be promising, as well as its possible use as additional source of the cholera vibrio enzymes and. as tripsin-like preparation, applicable for protein raw material hydrolysis in production of microbiological nutrient media.
G. A. Danchinova,
M. A. Khasnatinov,
E. V. Arbatskaya,
R. S. Shoboyeva,
S. S. Khankhareyev,
D. .. Abmed,
J. .. Bataa,
D. .. Tserennorov,
D. .. Otgonbaatar
206-209 453
Abstract
The results of the research of Selenga river basin as territory of highest risk of human infection with diseases with natural focality in Buryatia and Mongolia are present in this paper. Both in Buryatia and. in Mongolia the highest incidence rate for tick-borne encephalitis is associated with districts situated in the basin of Selenga river - Pribaykalskyi district and Selenge aimag respectively. All tick-borne infections and. every vector tick species detected in Buryatia and. Mongolia so far are present on the territories of these two districts. The epidemiological processes in the natural foci of these territories are shown to be very similar in spite of national and administrative borders.
Yu. P. Dzhioev,
G. V. Yurinova,
B. G. Sukhov,
S. M. Popkova,
T. V. Ganenko,
N. N. Pogodaeva,
D. K. Vasiljeva,
E. B. Rakova,
L. A. Safronova,
V. S. Podgorskij,
B. A. Trofimov
210-212 468
Abstract
Natural nanocomposites hemicellulose arabinogalactan and flavonoids isolated (from Siberian larch) and characterized. Additionally nitro- and sulfo-esters of arabinogalactan and its calcium salt are synthesized and. characterized. All of the derivatives of the beta-hemicellulose arabinogalactan. are water-soluble and are promising prebiotics on the example test-strain Bifidobacterium bifidum. (except for the nitrate esters of arabinogalactan).
Yu. P. Dzhioyev,
A. O. Revizor,
V. M. Yahnenko,
I. V. Kozlova,
E. K. Doroshchenko,
O. V. Lisak,
O. V. Suntsova,
O. O. Chernoivanova,
A. I. Paramonov
213-216 465
Abstract
Allozyme structure of the population of ticks Ixodes persulcatus in 8 enzyme systems is shown in two samples of ticks from the recreational areas in suburbs of Irkutsk. From the total of 13 loci, which encode these enzymes, eight were polymorphic. Based on allozyme analysis of populations of mites, it is found that most of them show intrapopulation heterogeneity and. the lack of difference between the two populations from different foci. A similar pattern may indicate a large rate of migration of genes between populations and. their close relatives.
217-219 415
Abstract
We surveyed. 112 adolescents aged 14-17 years. This cohort of children was divided into 3 groups: gifted realized, unrealized and secondary gifted intellectuals. The perinatal course peculiarities influence to development of psychosomatic disorders in gifted students. Perinatal factors such as age of mothers less 30 years, eventfullness of pregnancy and. deviations in child development up to 1 year were found in the students with psychosomatic disorders.
I. M. Zhulidov,
M. V. Antonycheva,
O. A. Lobovikova,
E. G. Abramova,
A. K. Nikiforov,
I. V. Shulgina,
L. V. Zaitseva,
N. P. Mironova,
N. I. Vakhrushina,
S. V. Astafieva,
V. I. Pavlova,
V. S. Bronnikova,
O. A. Volokh,
N. G. Avdeyeva,
G. V. Bazlov,
A. V. Komissarov
220-223 417
Abstract
The data on exploration of biological properties of experimental solid and liquid media on the basis of dry enzymatic hydrolysate of fibrinobtained from production waste of anti-rabies immunoglobulin is presened here. The culture media engineered meets the requirements of normative documents and. is highly competitive with the test medium in their qualitative characteristics. Suggested media can be used for V. cholerae cultivation, including submerged cultivation, in production of cholera preventive and. diagnostic preparations.
224-227 456
Abstract
In this paper the data for mortality from experimental infections and natural contamination in different species of poultry is presented. Mortality of natural contamination was identified during the epizootic of influenza in the Novosibirsk area in July 2005. We infected domestic geese, ducks and two breeds of chickens with highly pathogenic influenza virus A/goose/Krasnoozerskoye/627/05 (H5N1) for the experiments constructed.
M. N. Kireyev,
N. P. Gooseva,
T. A. Polunina,
A. L. Kravtsov,
S. A. Bugorkova,
N. A. Sharapova,
T. M. Taranenko
228-230 418
Abstract
This study shows that Yersinia pestis capsular antigen F1 conjugated to the CS nano-particles demonstrates apparent immunogenic effects. When injected to the test animals it was able to stimulate the production of specific antibodies under smaller antigenic load, it caused activation of the genetic apparatus in the immune system cells, and it stimulated hyperplastic processes in the lymphoid organs. Solutions of CS plus antigens appeared to be stable and caused neither general nor local responses in the test animals; they were easy to make use of, thus demonstrating their potentialities to be exploited as adjuvants in immunologic practices.
I. V. Kozlova,
M. M. Verkhozina,
E. K. Doroshchenko,
O. V. Lysak,
V. A. Borisov,
M. K. Tuvakov,
L. S. Karan,
S. E. Tkachev,
Yu. P. Dzhioev
231-235 500
Abstract
Genotyping of nine strains and nine isolates of tick-borne encephalitis virus RNA (TBE) isolated from patients in the Irkutsk Region and. the Buryat Republic in 1963-2009 years conducted. The investigations revealed that three TBE virus genotypes are involved in the etiology of the TBE: Ural-Siberian (genotype 3), Far Eastern (genotype 1) and. European (genotype 2). It is found that the TBE virus of Far East and. Ural-Siberian genotypes can cause disease with a wide spectrum of clinical manifestations: acute TBE (focal and. nonlocal forms), chronic TBE (encephalitic form, progressive course.) Prospects of application of molecular genetic techniques for the rapid indication and. identification of the TBE virus in the blood of sick people are shown.
N. N. Kochkalova,
E. G. Abramova,
A. K. Nikiforov,
M. N. Kireyev,
O. A. Lobovikova,
L. V. Savitskaya,
S. A. Badarin,
S. V. Generalov,
N. I. Kostyleva,
N. A. Sharapova,
Yu. V. Ivanov
236-238 416
Abstract
We presented the data concerning optimization of presentation and consumer container of anti-rabies immunoglobulin obtained, from horse serum.. During the experiments, ampoules and. flasks were used for primary packaging. They were filled within 5 ml of the preparation of freeze-drying. Comparative analysis of physical, chemical and. biological properties and. molecular parameters of freeze-dried and initial forms of the immunoglobulin. was carried out. Freeze-drying was demonstrated to promote stabilization of its properties and. prevent emergence of fragments and. aggregates during anti-rabies immunoglobulin storage.
239-242 378
Abstract
The possibility of using biologically active proton (2-hydroxyethyl) ammonium, ionic liquids of the general formula HN+RiR2(CH2CH2OH)3_n.•OOCCH,X(Ind)Ai; R = H, Me, X = O, S, SO; n = 0-2 as stimulators of growth S. aureus shows in the article. 24 strains isolated from patients with purulent-septic complications from the surgical department studied. There are shown a significant acceleration, of growth of S. aureus in a agar nutrient containing yolk-salt agar, the addition of stimulants tested at concentrations 4.10-6.10 wt. %. Proposed use of stimulants for the development of improved methods of rapid diagnosis of healthcare-associated infections caused by S. aureus, which will greatly reduce the time of issue analysis and. prescribe adequate treatment promptly sick.
243-244 453
Abstract
Here we present the results of the analysis of mt16S ribosomal RNA gene of two species of ticks from Irkutsk region - Dermacentor nuttalli and D. silvarum.. The ticks from are closely related to each other with 99-100 % identity of nucleotide sequence of mt16S rRNA gene. The phylogenetic analysis has shown that both species are closely related and. formed the separate clade together with specimens of D. nuttalli and. D. silvarum. from China.
245-249 456
Abstract
This article is devoted to investigation of prostaglandin E2 as potential immunomodulator of gull tapeworm. This parasite has an important epizootic and epidemiological significance in the Lake Baikal and. its basin. Using confocal microscopy, it was revealed that prostaglandin. E2 is produced and. secreted with specialized frontal glands in the organism of gull tapeworm. Using the method of leukocyte culture, the influence of prostaglandin. E2 on immunological reaction of fishes was investigated (by way of example of three-spined stickleback). This immunomodulator decreased the absolute number of leucocytes and. percentage of lymphocytes. Despite of total leukocyte respiratory-burst activity decreasing, respiratory-burst activity per 1 granulocyte remains constant.
250-253 384
Abstract
Using lectins of Acilact, clinical isolates of Lactobacillus from vaginal biotope were screened. Lactobacilli of the biotopes were classified to functional groups. Results indicate autoregulation signal properties of probiotic microbial lectins.
254-256 391
Abstract
Developed by us conception of micropathogen associate acting like communicative organism was proposed. It allows predicting some pathogen responses to the presence of systemic localized lectins of probiotics. The rules of eukaryotic pathogen behavior in the presence of cascade probiotic lectins are formulated. New criteria of pathogenicity are described. The data will provide understanding the relationships of microbiota in biotopes as well as constructing directed space cascades of microbiocenose landscapes for medical biotechnology.
M. V. Lakhtin,
V. M. Lakhtin,
A. V. Aleshkin,
S. S. Afanasiev,
Y. V. Kulakova,
Y. V. Belikova,
Y. V. Agapova
257-261 388
Abstract
This paper summarizes the results of electrophoretic and other studies of exopolymeric substances (EPS) of probiotic lactobacilli and. bifidobacteria of human origin. It finds that EPS of lactobacilli were significantly less produced and. low stained with fluorescent dye compared to bifidobacterial ones. For the first time, the systems of biosurfactants of bacilli and. bifidobacteria were identified. Also the pictures of physical layer EPS gradients of bifidobacteria were described for the first time. Part of EPS included cationic latent biosurfactants. Perspective features of EPS are described. The used approaches are suitable for both screening probiotic producers of EPS and. monitoring EPS consumption or degradation. Electrophoresis in pore gels can be used for obtaining directed 3D gradients of EPS associated with (glyco)proteins and. (glyco)lipids.
262-264 705
Abstract
Based the available data and our own researches materials on Echinococcus multilocularis infection of wild animals in the basin of Lake Baikal (Republic of Buryatia) are presented here. E. multilocularis circulated by the circuit: red fox, common wolf (definitive hosts) - rodents (intermediate hosts) - red fox, common wolf (definitive hosts). The range of intermediate hosts in wild fauna includes rodents of family: Muridae (brown rat), Сricetids (muskrat, northern red-backed vole, Mongolian gerbil), Squirrels (long-tailed ground squirrel).
265-267 423
Abstract
Based on original research we attempted to assess the range of hematological parameters of the hemopoietic system in the Baikal omul infected. D. dendriticum in the spawning period. The following effects were found: low functional activity of T-cell immunity, constant level of B-cell immunity; the intensification of erythropoiesis; cellular responses give evidences of endogenous intoxication. Overall, identified hematologic changes as in infected as in uninfected subjects of the Baikal omul proceeded were in adaptive criterias.
268-270 482
Abstract
Studying contamination of vegetables and fruit pathogenic and opportunistic enterobacteria was conducted. It is shown that in washouts from a surface of fruits and greens often enough there are representatives of investigated. group. At the same time, the bacteriological analysis of superficially processed fruits has revealed occurrence Enterobacteriaceae in vegetative fabrics where they exist in quality endophytic microorganisms. The received, results proved possibility of preservation of pathogenic and. is conditional-pathogenic bacteria in vegetative fabrics after sanitary processing that testifies that the products of a phytogenesis used. in food without thermal processing can be a source of an infection of the person.
271-275 417
Abstract
The study provides the results of AIV surveillance in Central Asia region during 2003-2009. We have analyzed. 2604 samples from wild birds. These samples were collected in Kazakhstan. (279), Mongolia (650), and Russia (1675). Isolated viruses from samples collected in Mongolia (13 isolates) and. in Russia (4 isolates) were described. Virological analysis has shown that 6 isolates belong to H3N6 subtype and. 5 isolates belong to H4N6 subtype. Two H1N1 influenza viruses, one H10N7 virus, two H3N8 viruses, and. H13N8 virus, which is new for Central Asia, have been also isolated. Samples were taken from birds of 6 orders, including several species, preferring water and semi-aquatic biotopes, one species, preferring dry plain regions, and one more species which can inhabit both dry and. water biotopes.
276-280 386
Abstract
The methods for the synthesis of 4-(4 '-nitrophenyl)-L-(+)-treo- and 2,8-dimethyl-4-(4 '-nitro-phenyl)-L-(+)-treo- 1-aza-3,7-dioxabicyclo-[3,3,0]octanes as well as iodomethylates of L-(+)-treo-, D-(-)-treo- and. D-(-)-, L-(+)-treo -4-(4 '-nitrophenyl)-1-aza-3,7-dioxabicyclo-[3,3,0]-octanes have been developed. It has been shown for the first time that optically isomeric iodo-methylates of 4-(4 '-nitrophenyl)-1-aza-3,7-dioxabicyclo[3,3,0]octanes possess high growth-stimulating activity with respect to meningococcus strains isolated from cerebrospinal fluid of patients thus indicating the possibility of these compounds application for qualitative diagnostics of meningitis. A method for the preparation of elective «Dry medium nutritium. ad. meningococcos siccum» for isolation and. cultivation of meningococci has been elaborated. Methods for the synthesis of novel potential stimulators of microorganisms growth have been worked out on the basis of biologically active aryloxy(arylsulfanyl)acetic acids and. waste of Levomecytin (Chloramphenicol) production, «L-treoamine».
281-283 416
Abstract
In April-June 2012 a collection and analysis of structure of Ixodes tick communities was performed in various biotopes at the Primorye Territory. Five tick species (Ixodes persulcatus Schulze, 1930, I. pavlovskyi Pomerantzev, 1946, Haemaphysalis concinna Koch, 1844, H. japonica douglasi Nuttall et Warburton 1915, Dermacentor silvarum Olenev, 1932) were revealed. It was shown that I. pavlovskyi was rare at the continental part of the Primorye Territory, but it often happened at the Russian island. On the Continent H. japonica douglasi prevailed over H. concinna in total collections. Correlation of these species at the Russian island was reversed. Distinctions in the structure of Ixodes tick communities, formed due to specificity of ecological conditions and. long enough isolation of the parasites' fauna at the island, could affect the ratio and. features of the causative agents of the human infectious diseases for which the carrier organism serves as the first environment.
A. K. Nikiforov,
M. N. Kireyev,
O. V. Gromova,
A. V. Komissarov,
S. A. Yeriomin,
O. A. Volokh,
Yu. A. Aleshina,
A. V. Gayeva,
T. L. Zakharova,
V. I. Pavlova
284-287 474
Abstract
We evaluated immunochemical, physical and biochemical properties of Vibrio cholerae 569B INABA protective antigenes, derived on industrial and own-developed bioreactors as well as by technology of its concentration by tangential ultrafiltration. We detected, protease, twinase and. lysophospholipase in all samples. Also, dotimmunoanalysis showed equal concentration, of cholerogen-anatoxine and. O-antigen in all samples too. Using chromatography and. electrophoresis, we found their properties as similar. Thus, we suppose to be possible using developed bioreactor as well as technology of Vibrio cholerae 569B INABA protective antigens concentration by tangential concentration during a process of synthetic oral cholera vaccine production.
288-292 392
Abstract
Intestinal, nasopharyngeal microbiocenoses peculiarities and bifidobacteria species composition in children living in the Irkutsk region industrial cities under existing technogenic loading conditions have been studied. Major microbiocenosis variants in children living in the cities with the different levels of the atmospheric air pollution have been determined
VARIANCE OF BIOLOGICAL PROPERTIES OF OPPORTUNISTIC STAPHYLOCOCCUS UNDER NEGATIVE AERO IONS INFLUENCE
293-295 410
Abstract
The aim. of this research was to study some changes of staphylococcus biological properties under negative aero ions influence and lactobacillus. We applied a disco-diffuse method for detection of bacterial sensitivity to antimicrobials and. evaluated of the minimal inhibitory concentration by bacteriologic analyzer. Then we studied impact of negative aeroions flow on both isolated staphylococcus culture and. mixed with lactobacilli. Aeroions flow inhibits staphylococcal growth, decrease its sensitivity to antibiotics in vitro both in isolated culture, and. mixed with lactobacilli. Combined application of probiotics and. negative aeroions could be a new approach for changes of antimicrobial sensitivity.
296-299 436
Abstract
The paper is devoted to the long-term dynamics of Baikalian omul contamination with plerocercoids of Diphyllobothrium. dendriticum during the spawning migration into the Selenga River. Contamination peacks were in 2005 and 2011. Dynamic of diphyllobothriosis morbidity in Buryatia Republic in 2000-2011 have shown increasing trend of helm.inth.osis with 5-year cycle.
300-303 407
Abstract
Antagonistic properties of bifidobacteria to opportunistic microorganisms in vitro and mechanism of antagonistic activity decreasing were studied and. analyzed. Great percentage of bifidobacteria regional population strains with low antagonistic activity to transitional opportunistic microorganisms is registered. It results to colonization. resistance decreasing and. requires development of the preventive probiotics therapy methods to correct.
304-309 431
Abstract
For the first time in the south of the Central Siberia carried out large-scale study of wild and synanthropic birds for the presence of influenza A viruses. Along with the traditional methods of laboratory study of pathogens in Merskey's reaction and. PCR carried out ornithological research in areas of high concentrations of birds and. flyways. Also studied the long-term dynamics of isolation of positive samples containing specific antibody to influenza virus A subtypes H5 and. H7 and. genomic RNA of these subtypes. It was found that infection and. reinfection. of birds by avian influenza doesn't take place only during the wintering grounds, but also the breeding season in the water keepings of the south of the Central Siberia. Chicks are actively involved in the epizootic process in July, when transovarialy transmitted maternal antibodies disappear. Because of the peak interval of positive samples selection in Merskey's reaction and. PCR is 3 years, it is possible to organize an immunological monitoring followed by preventive programs development of birds deaths.
A. P. Savchenko,
V. I. Emelyanov,
P. A. Savchenko,
S. O. Andreyev,
I. A. Savchenko,
N. V. Karpova,
V. L. Temerova
310-315 417
Abstract
It was studied the long-term dynamics of influenza A viruses circulation in the Southern Central Siberia. Also, we studied of birds population dynamic as the main carriers of viral infection, as well as spatial and temporal distribution of positive samples containing specific antibody to influenza viruses subtypes H5 and. H7 and. genomic RNA of these subtypes. It was considered the cases of mortality wild. and. synanthropic birds in the region. It was shown a correlation between the identified centers of the virus influenza, the deaths of birds and. human disease with significant excess seasonal epidemic threshold, including a subtype Н1NЫЦ.
316-318 392
Abstract
During this research huge surveillance study, the role of Gulls as the nature host of influenza A viruses was established. Oral and cloacal samples from Gulls were collected on the territory of the Russian Far East. Fourteen influenza A viruses were isolated.
319-322 401
Abstract
During avian influenza virus (AIV) surveillance in Russia, 2008, H15N4 subtype of the virus was isolated. All the H15 viruses had been previously isolated in Australia in 1979 and 1983. This is the first report about isolation. of AIV H15 subtype elsewhere. Genetic and. antigenic analyses were made. The significant antigenic differences between A/teal/Chany/7119/2008 strain and. reference strain A/shearwater/ Australia/2376/1979 were revealed. The results of this study show the necessity of monitoring for avian influenza viruses, study of pathogen genetic variability, evolution and ecology.
323-324 481
Abstract
A novel original approach to self-organization of hybrid nanobiostructures possessing unique complex of physicochemical and biological properties (extremely demanded for noninvasive diagnosis and. therapy) is discussed.
325-327 435
Abstract
Determination of ticks species is very important for epidemiology and ecology investigations but in some cases the morphological study couldn't be carried out. In this work the PCR system based on species-specific primers to cytochrome oxidase subunit 1 (COI) gene sequence was developed for molecular species typing of Ixodes persulcatus and. Ixodes pavlovskyi ticks.
328-330 451
Abstract
Data on infection of muskrat by trematoda species Quinqueserialis quinqueserialis in the Selenga River Delta are presented in this paper. The indexes of infection of muskrat by parasite in 2011 are similar to those in 1981 and 1982. The study is the first attempt to assess the influence of infestation on structure and. quantity leucocytes of blood of infected muskrat in Lake Baikal basin.
M. A. Khasnatinov,
S. Havlikova,
A. Tuplin,
M. Kazimirova,
N. Goonavardane,
M. Slovak,
B. Klempa,
I. M. Jones,
M. Labuda,
E. A. Gould,
T. S. Gritsun
331-334 493
Abstract
There are 3 subtypes of tick-borne encephalitis virus (TBEV) distinguished, at present time: European (EU), Siberian (SIB) and Far Eastern (FE). The former one is associated with Ixodes ricinus tick, whereas the latter two - with I. persulcatus. The circulation of TBEV in nature is mediated by the non-viraemic transmission, between infected and. uninfected ticks co-feeding on the same hosts and. it was shown that transmission rate of «Hypr» strain (EU subtype) is much higher than rate of «Vasilchenko» strain (SIB subtype) - 60 and 5 % respectively. To reveal the viral determinants of transmission efficacy, we constructed the series of recombinant viruses with gradually exchanged genes coding structural (str) and. non-structural (ns) viral proteins. The recombinant virus Hypr[str]Vs[ns] achieved the rate of non-viraemic transmission of 70 %. The introduction of separate structural genes of Hypr into Vs infectious clone has enhanced the transmission efficacy as well, though not to such extent as entire structural region but up to 33 % only. Thus, it was shown that efficacy of non-viraemic transmission of TBEV depends from properties of viral structural proteins mainly.
335-339 377
Abstract
During assessment of water quality in the lake Kotokelskoe, we observed some cyanobacteria that forming harmful to human and animal toxins. According to the chlorophyll a content, the lake Kotokelskoe is eutrophic with high productivity of phytoplankton. But microbiological parameters such as total number of microorganisms and saprophytes cause to classify this lake as «polluted waters». Preparation. «Baikal EM 1» is actively involved to the processes of self-purification of water. It could provide decreasing of chlorophyll content total number of microorganisms in aquarium water.
E. A. Tchaporgina,
G. A. Danchinova,
M. A. Khasnatinov,
A. V. Liapunov,
S. V. Pyzhjanov,
E. V. Arbatskaya
340-342 444
Abstract
The California encephalitis serogroup virus, Batai virus and Geta virus were isolated as result of investigation of distribution of arboviruses in Pribaikalye. The results of the study of influenza A virus in birds in Pribaikalye are presented as well.
343-346 420
Abstract
The work presents the results of epizootological monitoring of mass gastro-intestinal diseases of young poultry in the Irkutsk Region. As a result of the poultry sickness rate analysis covering the period, of 2006-2010 the following nosological units have been distinguished: colibacteriosis (37,3 %), salmonellosis (25,4 %), pasteurellosis (19,8 %), spirochetosis (14,4 %), staphylococcus diseases (2,9 %), infectiouslaryngotracheitis (0,2 %). The results of the monitoring research, indicated that gastro-intestinal diseases are caused, by a wide range of opportunistic pathogenic microflora agents. However, these bacteria groups are mostly represented by microorganisms Enterobacteriaceae family of Escherichia and Salmonella types. The research established not only wide diversity of the distinguished serological colon bacillus and. salmonella varieties, but also fluctuations in their proportion in the common structure of the distinguished culture in the examined time interval.
N. A. Sharapova,
M. N. Kireyev,
E. G. Abramova,
S. V. Generalov,
Zh. V. Matveyeva,
I. M. Zhulidov,
N. E. Tereshkina,
E. A. Mikheyeva,
L. V. Savitskaya,
A. V. Gayeva,
A. K. Nikiforov
347-350 468
Abstract
Described here are the results of glicoproteid isolation from the fixed rabies virus, strain «Moscow 3253», using non-ionic detergent with subsequent chromatographic purification. The obtained antigen was demonstrated to be applicable as immunoreagent for construction of diagnosticum, by means of conjugation with colloid gold nanoparticles. The diagnosticum is meant for detection of specific antibodies in immune sera of horsesproducers, and in the preparation of anti-rabies immunoglobulin, in dot-immunoassay.
351-355 442
Abstract
Genetic evidence of shrew-borne hantavirus in Russia is presented here. Impressive distribution of a hantavirus Seewis (SWSV), previously discovered in the Eurasian common shrew from Switzerland, was demonstrated in Siberia among closely related shrew species: Sorex araneus, S. tundrensis and. S. daphaenodon. SWSV circulation was shown in Altai Republic, Krasnoyarsk Krai, Kemerovo and. Novosibirsk regions, surburbs of Novosibirsk and Irkutsk Cities.
ORGANIZATION OF PUBLIC HEALTH SERVICE
356-359 411
Abstract
The article presents the analysis of the characteristics of the epidemiological process disease of hepatitis A in the Republic of Buryatia. Patterns of epidemic process of viral hepatitis A on the territory of the Republic of Buryatia were identified. Characteristics of outbreak sickness rate, results genotyping and sequencing of selected viruses are presented. The task and. anti-epidemical work on the prevention of HAV was explored.
360-362 479
Abstract
A differentiated approach to the organization of measures for sanitary protection of the territory, based on the analysis of the transport and. facility components of risk emergency situations in the field of sanitary-epidemiological welfare of the population is proposed. The total evaluation of the external and. internal components of the risk emergency situations for each municipality of a region of the Russian Federation allows to determine type of readiness to conduct activities of the sanitary protection of the territory for a given area. The ranking of the region of the Russian Federation will contribute to the achievement of optimum readiness of the Institutions of Public Health and. sanitary-epidemiological surveillance at the level of municipalities to the occurrence of diseases, requiring measures of sanitary protection.
363-367 411
Abstract
The results of yersinioses microbiological monitoring in Novosibirsk Region are presented and introduction of a quality management system in activities of a laboratory of especially dangerous infections is experienced.
368-371 409
Abstract
This study presents analysis of the timely detection of pulmonary tuberculosis in 1334 patients who underwent treatment in the Regional TB Dispensary N1 in Krasnoyarsk city in 2010-2011. Late detection of tuberculosis was established in 60,2 %, and factors which are contributing to it were as following: the male, living in rural areas, social vulnerability, and. violation of the regulated terms of X-ray examination. The significance of late diagnosed patients to increase the reservoir of tuberculosis infection and spread of drug-resistant strains among the population was shown. It was determined that an increase in coverage of population by preventive X-ray examinations resulted in improvement of quality indicators only in 2,6 % of active detection of pulmonary tuberculosis in the form of growing proportion of the patients revealed during preventive examinations timely, as well as reducing proportion of fibro-cavernous pulmonary tuberculosis and. post-mortem diagnosis of tuberculosis among the newly diagnosed patients. During the active detection of TB among population with a focus of work on real, established during the research, its groups the conclusion about the need to use the obtained results was made.
372-374 407
Abstract
This article presents an analysis of disease trends in selected age groups of the population of Irkutsk for the period of 1992-2009 and it is found that most of these trends are dependent on socio-economic factors. Built polynomial regression models revealed significant increase in morbidity of mental disorders in children, diseases of the nervous system and. the digestive system against opposing change prevalence of adolescent and. adult population for the analyzed period.
375-378 420
Abstract
We present the results of treatment of patients with primary multidrug resistant pulmonary tuberculosis (MDR PT) (main group) and with acquiring MDR PT (comparison group) in Republic of Buryatia. We used the indexes for estimation of the treatment results according to the international standard. Index of sputum clean negativations is higher in main group - 77,8 %, in comparison, group - 44,4 %. The rate of negative sputum, in main group was 78 %, in comparison group - 41,9 % (р < 0,01). Increasing of drug resistance to reserved, medication up to MDR were often in the 2nd group (21/10,3 %). Tolerability to reserved, medication, in the 1st group was better than in the 2nd group (28 % and. 43,4 % correspondingly, р < 0,05). In 24 months there were 26 % recovered patients in the 1st group and. 10,8 % in the 2nd group (р<0, 01). Negative treatment result was observed only in the 1st group (n = 5, 2,5 %). Treatment was interrupted in 18 % of the 1st group of patients and. in 28,1 % of the 2nd group of patients. There were 21 patients died in the 2nd group (10,3 %). We suppose that in the cases of tuberculosis the treatment results could be improved by the early diagnose of MDR and. balanced anti-tuberculosis treatment.
REVIEWS OF LITERATURE
379-381 436
Abstract
Rickettsiales bacteria are obligatory intracellular parasites in vertebrates and/or invertebrates, and some cause severe febrile illness in humans as well as livestock. In Japan, "Rickettsia japonica" and "Orientia tsutsugamushi" are well known as causative agents for "Japanese spotted fever" transmitted by ticks and. "Tsutsugamushi disease" transmitted by mites, respectively. In recent years, many researchers have reported the presence of several tick-associated. Rickettsiales bacteria in Japan, mostly by molecular-based analyses. Some of these might be public health or veterinary significance. Such. Rickettsiales bacteria include spotted fever group rickettsiae other than R. japonica, Ehrlichia spp., Anaplasma spp., and. Neoehrlichia sp. We will summarize and. introduce the representatives of these Rickettsiales bacteria present in Japan.
382-385 535
Abstract
The review of literature and own data analysis describe bacteriophages application for medical microbiology (especially in cases of multiple drug resistance of pathogens) and. industrial biotechnology (the problems of decontamination and. stabilization of foods). Prospects of bacteriophages for enzyme therapy are underlined.
386-388 445
Abstract
Over the past 45 years, more than 30 episodes of epizootic diseases among birds were described. Some of them caused human diseases, often with high mortality rate. The most crucial cases of influenza outbreaks appeared in Europe and in the USA. Avian influenza virus types of subtype H5, which are the source for the avian influenza virus subtype H5N1 presented nowadays were first isolated from a goose in 1996 and. from the deceased person in 1997 in Hong Kong. Seasonal flu outbreaks usually appear firstly in the East, and. then move to the West. Viruses, therefore, detected at early stages in Asia are analyzed and. used to predict the components, which are applicable in the vaccine preparation for the next flu season. Over the past 50 years, genetic information about constantly changing circulating influenza strains, gratuitously received through viruses data communications and. exchange between countries, and. about epidemiological tendency to infection spreading has been gathered by the wide surveillance network (Global Influenza Surveillance Network), under the guidance of WHO.
M. B. Sharakshanov,
N. V. Breneva,
A. K. Noskov,
E. Yu. Kiseliova,
S. A. Kosilko,
T. T. Shkaruba,
M. V. Chesnokova
389-392 395
Abstract
In this study the increased number of the imported cases from endemic countries or occurring of the disease in earlier unknown natural leptospirosis foci in the world among participants of various extreme competitions, hostilities and travelers was revealed at analysis of the epidemiological leptospirosis situation. New human risk groups subjected to infection were determined.
393-396 363
Abstract
Russia covering a large part of Eurasia is of special interest for influenza virus ecology and evolution. The importance of this region has been confirmed by the previous outbreaks of HPAI H5N1 among wild birds from 2005 to 2010. Avian influenza virus surveillance is an important task prior the fundamental study and introduction. these data in practice. The article contains detailed comparative virological, molecular, pathogenic characteristics of H5N1 viruses isolated in Russia. Some molecular-epidemiological aspects are discussed. This study demonstrates the need for ongoing surveillance to detect new variants of influenza viruses and. facilitate prevention of outbreaks.
ИНФОРМАЦИЯ
ISSN 2541-9420 (Print)
ISSN 2587-9596 (Online)
ISSN 2587-9596 (Online)
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