The dynamics of the level of malondialdehyde (MDA), activity of superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase was investigated in blood serum of professional rugby players during one year training and competitive season. The MDA level significantly exceeds control level during all the year. The maximum of MDA concentration is registered at the end of competition period and isn't restored during the period of rest. The SOD and CAT activity is significantly reduced and doesn't return to control levels during the year. These changes prove chronic decompensation in the antioxidant defense system. In athletes glutathione-S-transferase is the leading enzyme realizing protection against reactive oxygen species in blood serum of sportsmen. Compensatory increase of its activity is 660 % in reference to the level of control group.

Changes of haematological and biochemical indices depending on the nosological entities of occupational diseases and the gender were analyzed in workers of chemical complex. Changes of these indices depending on the gender identity were revealed in workers with skin hyperkeratinization at the conduction of medical examination. Eosinophilia, lymphocytosis and leukopenia were revelaed in men with hyperkeratinization, increase of hemoglobin quantity and leukopenia were revealed in women with hyperkeratinization. There were no differences depending on the gender in patients with skin cancer.

The article reviews new laser-based methods of treatment of basal cell skin cancer. The results of the treatment of 25 patients with skin cancer were analyzed. The article reviews problems of complicated course of the disease when surgical and X-ray methods of treatment are not enough. We started to apply photodynamic therapy with use of Radahlorin for the enhancement of the results of treatment of recurrent basal cell skin cancer. New method of treatment became rather effective at the treatment of recurrent basal cell skin cancer.

The aim of the research was to study localization of wandering focus and effectiveness of extensive endocardectomy at left ventricular reconstruction in patients with ischemic heart disease (IHD) with postinfarction left ventricular aneurysm (LVA) in combination with ventricular tachycardia (VT). Examined patients besides diagnostic routines had intracardiac electrophysiological examination (EPE) with use of 3D-navigation system CARTO^TX (Biosense Webster). During EPE abnormal zones of delayed navigating and zones of double potential with main localization in the area of cacuminal and middle septate segments were found. All patients were divided into two groups: patients of the first group had aortocoronary bypass (ACB) in combination with left ventricular reconstruction completed with extended resection of endocardium with pinch of all cacuminal and middle septate segments; 2 patients of the second group had isolated ACB, 2 patients had ACB with mitral annuloplasty with rim and de Vega plasty of tricuspid valve, 1 patient had percutaneous transluminal coronary angioplasty, 10 patients hadACB in combination with LVA plasty without extended endocardium resection. As the result of the treatment in the first group there were no VT episodes after resection of aneurysm with extended endocardium resection; 33 % of patients in the second group had implantable cardioverter defibrillator, 7 % of the patients had radio frequency ablation of VT nidus, 7 % of the patients had anti-arrhythmic therapy. Taking into consideration that main localization of ectopic nidus in patients with IHD with postinfarction LVA and VT is in the area of cacuminal and middle septate segments, left ventricular reconstruction in these patients needs to be conducted with extended resection of endocardium of these segments.

The aim of the research was to study possibilities of MR-imaging for estimation of posttraumatic changes in the knee joint. 660 patients with posttraumatic processes in the knee joint were examined, all of them had magnetic resonance imaging with use of tomographic scanners of various classes with magnetic field intensity from 0,5 to 1,5 TL. Abilities of MR-imaging in the estimation of ligamentous apparatus of knee joint at posttraumatic changes are showed. MR-imaging symptoms of changes of fibrocartilagenous, ligamentous, soft-tissue and bone structures at the injuries and posttraumatic complications of ligamentous apparatus of knee joint were specificated and completed that was the basis of differential diagnostics. The comparative assessment of MR-imaging and knee joint arthroscopy was also conducted. On the basis of principles of evidentiary medicine we showed the indices of the knee joint MR-imaging effectiveness at the posttraumatic changes. MR-imaging accuracy is 96 % for diagnostics of meniscus injuries, 90,5 % for diagnostics of ligaments injuries, 71 % for the changes of synovial layer and 37 % for the changes of articular cartilages.

The aim of the research was to assess the reactivity of female organism with hypertrophic scars. 42 women of 16-35years were examined on the 5-7^th day of the menstrual cycle, 17 of them had normotrophic and 25 - hypertrophic scars. The quality of scars (according to the clinical and morphological characteristics), white blood cells, blood lipids, hormonal and immune status were assessed. Non-parametric Mann - Whitney U-test and discriminant analysis were used at statistical processing of the results. Average values and standard deviations were also calculated for interpreting the results of quantitative indications. As the result of discriminant analysis the most informative indicators of reactivity of an organism that allow to differentiate patients of the studied groups were defined for patients with normotrophic and hypertrophic scars. It was concluded that the imbalance of steroid hormones (progesterone, cortisol, testosterone, estrogen) was important in the etiopathogenesis of hypertrophic scars.

An anticholinesterase preparation was introduced in 3 patients 30 minutes before direct electrical stimulation with low-intensity current in order to reduce treatment time, to increase muscle tension and to decrease sensitive disorders in the zone of innervation of the brachial plexus and peripheral nerve. 1,0 ml of 0,05% proserin solution was injected. Once reaching maximum concentration in blood proserin allowed to achieve potentiating the effect of electrical stimulation, increasing patient's muscle strength and independent muscle contractions, as well as 0,18 mV, on the average, increasing voluntary activity of the muscles tested according to EMG results which corresponds to 37-45 % of physical activity of the tested muscle group. The proposed method allowed to reduce treatment periods, to regress focal symptoms, to increase muscle tension and to decrease sensitive disorders in the zone of peripheral nerve innervation.

The article reviews successful experience of brachial plexus blockade under ultrasound control and describes this technique. The authors give evidence, that using both ultrasonic imaging and neurostimulation during brachial plexus blockade allows to improve significantly (by 31,6 %) quality of blockade (p, = 0,01) and to decrease (by 21,4 %) the risk of purposeless vessels puncture (p= 0,02).

The authors studied remote results in 236 (83,7 %) of 282 patients who had hip replacement. 195 (82,6 %) patients had good results, 38 (16,2 %) - satisfactory, 2 (0,84 %) - unsatisfactory (due to the development of suppurative complications). Lethal outcome was registered in 1 (0,8 %) case (myocardial infarction). 16 patients had bilateral hip replacement. Authors divided rehabilitation measures into local (aimed to the restoration of limb's functions) and general and also by the periods. Precise compliance with standards at the endoprosthesis replacement with further optimal selection of specific physical factors and exercise therapy allow to recover patient to active and qualitative life.

We studied the results of treatment of 167 patients with postoperative ventral hernias (POVH) with use of mesh endoprosthesis. Basic group included 22 (13,0 ± 2,6 %) patients, who had plasty of anterior abdominal wall with use of proposed method. This method is a modification of «on lay» plasty. The first group of clinical comparison included 95 (57,0 ± 3,8 %) patients operated with «on lay» method. The second group of clinical comparison included 50 (30,0 ± 3,5 %) patients operated with «sub lay» method. Morphological study of hernial sac was conducted, the indications to the new method of plasty were revealed, its efficiency in comparison with «on lay» plasty was proved. The article reviews frequency and character of complications and quality of life of patients after application of various kinds of prosthetic plasty. We developed an algorithm for choosing the method of abdominal wall plasty.

The article presents the results of study of antibiotic resistance of bacteria isolated from the lake Baikal ecosystem. It was revealed that in the littoral zone of the lake Baikal (area of Listvyanka, Baikalsk, Slyudyanka) under the anthropogenic impact there are strains of microorganisms resistant to a wide range of antibiotics. Strains resistant to all antibiotics are 1 % of the isolated bacterial strains; strains sensitive to all antibiotics are 6 %; strains isolated from the drill samples of bottom sediments are 80 %. This phenomenon can be considered as an indicator of anthropogenic influence.

The article presents sanitary-hygienic assessment of the state of air environment of chemical industries of Eastern Siberia of production of methyl alcohol and methylamines, butyl alcohol by oxosynthesis, methyl-tert-butyl ether (MTBE). Productions use continuous production cycles with high degree of automation and mechanization of laborintensive operations. Retrospective study of work area of these productions for 10-year period showed absence of excess of occupational exposure limit of average annual concentration of repugnant substances (methanol, carbonic oxide, methylamines, butanols, MTBE, aliphatic hydrocarbons С₁-С₁₀ et al.). Also significant tendency for its decrease during the period of monitoring was registered, that is connected with introduction of organizational-technical and hygienic measures conductive to the sanitation of working conditions on studied productions. Researches conducted at the present day testify to the persistent influence on the employees of the complex of chemical compounds in low concentrations. Nevertheless despite parameters of industrial environment meet hygienic requirements it's necessary to take into account combined influence of risk factors on the employees for prevention occupational diseases.

We studied quality of water of centralized water supply sources by microbiological and sanitary chemical indices in Aldansky, Khangalassky and Megino-Kangalassky districts of Sakha (Yakutia) Republic. The article presents comparative assessment with the same indices of Sakha (Yakutia) Republic. Statistical accountforms of the Center of Hygiene and Epidemiology in Sakha (Yakutia) Republic were used for the analysis. 14133 water samples were analyzed during 2006-2012. It was showed that the mostfavorable situation of the conformance to the sanitary chemical indices of water samplesfrom centralized water supply sources was in Aldansky district (specific density of unsatisfactory samples was 7,9 % and was lower than in the republic as a whole). The highest percentage of irregular samples of water exceeding the level of Sakha (Yakutia) Republic was registered in Khangalassky district (32,3 %). This index in Megino-Kangalassky district was at the level of the republican one (14,1 %). In long-term period in Aldansky district the dynamics of decrease ofunsatisfactory by microbiological indices samples of water that reflects situation in Sakha (Yakutia) Republic as a whole; in Megino-Kangalassky district we registered increasing dynamics. The highest specific density of water samples irregular by sanitary chemical indices was revealed in Khangalassky and Megino-Kangalassky districts (41,8 % and 29,2 % respectively); the lowest - in Aldanky district. In long-term period number of water samples inconsistent to the hygienic standards by sanitary chemical indices was defined by the increase in the other half of analyzed period.

Experimental simulation of pathological processes is of greatest importance for perception of essence of various kinds of surgical pathology, for deciphering etiology and pathogenesis of disorders and finding possible ways of their management. The goal of an experimental research is systematization, strengthening and extension of theoretical and practical skills and experiences, and practicing their application in solving scientific problems. To perform an experiment one needs to have research skills, knowledge of modern techniques of setting up an experiment, evaluation and interpretation of its results, to be able to make public speeches at conferences, to present the case in the form of an article or an abstract, to work with scientific references, to compose priority documents (patents and innovation proposals). Methodology is an algorithm of goal search, set of techniques, methods, measures and principles of goal achievement. Peculiar feature of experimental researches involving laboratory animals is high moral responsibility, in particular connected with humane treatment of animals, and this responsibility falls both on the performer and on his research adviser.

The work presents evaluation of influence of plant preparation «Thyreoton-forte» on endothelium of vessels and morphological structure of thyroid gland in experimental hypothyreosis. Experimental hypothyreosis in rats was induced by administration of mercazolilum (10 mg/kg, intragastric introduction) during 28 days. The course administration of «Thyreoton-forte» (50,100 mg/kg, intragastric introduction) decreases the number of circulating endothelial cells (p < 0,05) and normalizes the structure of thyroid gland. Pharmacological effect of «Thyreoton-forte» is provided by protective influence in vascular endothelium and positive action on thyroid gland.

The program of mathematical processing of the electrophoregramm of protein strips is suggested. It is intended for definition of weight of proteins in a researched sample and has the purpose to automatically establish coordinates of the maximal concentration of proteins in investigated strips and to calculate the area under their peak using the algorithm of integration with an adaptive choice of a step, after reading the scanned strip of electrophoresis with expansion (.pcx) and processing of the received digital matrix.

The objective of the study was comparative study of the effect of VEGF and FGF on fibroblastic phase of inflammation by replacing necrotic myocardium on its model of acute myocardial infarction. Model of myocardial infarction in rats was carried out to study the effect of increasing and decreasing concentrations of basic fibroblast growth factor (FGF2) and vascular endothelial growth factor (VEGF) on the dynamics of morpho-metric parameters fibroblastic phase. It has been established that the increase of VEGF in the systemic circulation more stimulated the growth of fibroblasts compared to FGF. Thus, the density of fibroblasts in the repair area in VEGF group during all periods of observation values in a group exceeds FGF. Differences were significant at the 7^th (295 [249-340] versus 205 [192-275], p = 0.0257) and the 30^th day (132 [125-148] versus 88 [81-122], p = 0.0036). However, when reducing the amount of growth factors by introducing antibodies fibroblastic cells showed greater sensitivity to the reduction of FGF. Fibroblast density in the area of repair in the group at all time points antiVEGF observation group was significantly higher performance antiFGF: the 3^rd day 299 [130-481] and 132,5 [92-148], p = 0,0257; the 7^th day - 306 [272-369] and 167 [151-215], p = 0,0008; at the 14^th day - 250.5 [233-260] and 131,5 [121-147], p = 0,0002; on the 30^th day - 195.5 [139-237] and 88,5 [81-96], p = 0.0003 respectively. It was established, that modifying the natural regulatory stimulation of both the side with the additional administration the growth factors and decreasing its level at the expense of elimination upon binding to antibody leads to disruption of fibroblast differentiation to fibrocytes.

The article presents the results of placebo-controlled research of the effectiveness of magnetic infrared laser therapy (MIL-therapy) of patients with psoriasis with malabsorption syndrome. The methods of treatment (traditional method, method with the use of magnetic infrared laser therapy and placebo control) were compared. The results of study showed that the group of patients who were treated with use of magnetic infrared laser therapy had more significant positive dynamics.

We realized pharmacognostic study of 6-component composition recommended as a therapeutic and prophylactic agent at the hepatic and bile passages disorders, for the accelerated elimination of products of kidneys' nitrogen metabolism and for normalization of lipid metabolism. The norms of indices defining composition's quality and also significant macro- and microscopic characteristics are determined. As the result of phytochemical research we identified main groups of biologically active substances.

The article presents the results of study of amino acid composition of Orthilia secunda L. and Pyrola rotundifolia L. in Eastern Siberia. It was established that studied plants contain 15 nonessential and 9 essential amino acids. Concentration of amino acids in the aboveground part of Orthilia secunda L. was 55,92 mg/g, in the grass of Pyrola rotundifolia L. - 64,02 mg/g. The largest part of amino acids of Orthilia secunda L. and Pyrola rotundifolia L. are similar representatives of this class. Among nonessential amino acids content of glutamic (6,66 and 7,40 mg/g), aspartic acid (3,93 and 4,92 mg/g), glycine (2,96 and 3,43 mg/g) is expressed. Essential amino acids are represented by the following series: leucine - 4,49 and 4,76 mg/g, lysine - 4,19 and 4,30 mg/g, phenylalanine - 3,05 and 3,08 mg/g.

We determined promising sources of phytoecdysteroids - Rhaponticum uniflorum (L.) and Serratula centauroides (L.), growing in the Buryat Republic. Biologically active substances were founded: ecdysteroids, phenolic compounds, polysaccharides, flavonoids, tannins, saponins, vitamins.

The work presents the basic results of researches of Scientific Center of Reconstructive and Restorative Surgery SB RAMS on the issue of influence of growth factors on postinfarction remodeling of myocardium. It has been shown, that the researches defined the significance of vascular endothelial growth factor (VEGF) and fibroblast growth factor (FGF) for myocardium reparation after its ischemic damage. There is a description of mechanisms of regulation of bloodflow in distal parts of coronary vasculature by endothelin on different stages of formation of postinfarction cardiosclerosis. Quantitative characteristics is given of mitogens level dynamics, cell composition and activity of developing connective-tissue component in various parts of myocardium on stages of reparation after ischemic damage. The article also contains substantiation of perspective of management of growth factors dynamic for providing optimal parameters of regeneration area and improvement of pathological process outcomes.

Article shows the analysis of the results of scientific research in the sphere of epidemiology of Helicobacter pylori infection in different ethnic populations including Russian ones: the peculiarities of infection prevalence, structure of associated diseases in adults and children. There is a review of mostfrequently discussed data on the factors influencing the characteristics of Helicobacter pylori-gastritis dynamics in ethnic populations.

The article presents a review of literature devoted to the epidemiology of HIV infection in the world and Russia, as well as the investigation of reproductive disorders in women with HIV infection and viral blood-borne hepatitis. Actuality of the problem lies in the fact that grows the number of HIV infected and having blood-borne viral hepatitis with involvement in the epidemiological process people of reproductive age, and especially the increase in the number of HIV-infected women of childbearing age. Importantly, it is necessary to predict, diagnose and correct reproductive disorders in women, taking into account basic patterns and risk factors for the formation of such violations with a view to the restoration and preservation of reproductive capacity and ability to have healthy children.