The article presents the results of complex treatment of chronic recurrent aphthous stomatitis by methylcellulose-based gel with arabinogalactan biocomposite containing zero-valent silver in nano-valent condition. The gel was applied 3-6 times a day at the prescription of Diquertin 0,06 g 3 times a day and Fibrolar 0,3g 3 times a day. The proposed method of treatment allows to improve the effectiveness of treatment by increasing remission and reducing treatment terms.

There are many causal factors infringing reproductive health of men, including overweight that is associated with dysfunction of such system as lipid peroxidation and antioxidant protection. According to the results of study of two groups of infertile men with different body mass index we found that men with excess body weight have more pronounced imbalance in the system of lipid peroxidation compared to infertile men with normal body weight. As a material for biochemical studies we used blood serum. Content of substrates for lipid peroxidation - the compounds with isolated double bonds and products of lipid peroxidation processes (diene conjugates, ketodienes and conjugated trienes, thiobarbituric acid active substances) - were determined on spektrofluorofotometre «SH1MADZU-1501» (Japan). At the analysis of intergroup differences for independent samples parametric Student's T-criterion was used.

Magnesium is one of the important elements, necessary for the most of biochemical reactions, including those in nerve tissue. Magnesium insufficiency takes a leading position in the structure of elemental status pathology. 20-63 % of females of 18-45 years have borderline or moderate magnesium deficiency. Earlier conducted studies showed decreased level of magnesium in blood serum of women using combined oral contraceptive pills (COCP). We examined 137 young females of reproductive age (average age -19,1 ± 1,1 years) and defined the level of magnesium in blood serum and 24-hour urine at the background of taking COCP. Depending on the level of magnesium in blood serum all women were divided into 2 groups. Females who planned to use COCP and had initially normal level of magnesium in blood serum (n = 88) were randomized to form groups of observation and prophylaxis (with the use of magnesium preparations). All women with reduced level of magnesium (n = 49) together with hormonal contraceptive were prescribed magnesium preparation in combination with pyridoxine. The women with preventive use of magnesium-containing preparation had no significant reduction of magnesium level either in blood or in urine. And the women with reduced Mg level who were taking COCP and received magnesium-containing preparation in combination with pyridoxine had increased level of magnesium in blood serum and 24-hour urine. Thus, prescription of magnesium preparations in combination with pyridoxine to young women of reproductive age using combined oral contraceptive pills has been proved reasonable for reducing the risk of magnesium deficiency.

The research is dedicated to the study of characteristics of health of children, whose relatives of 1^st and 2^nd lines suffered thrombosis of vessels of different caliber and localizations before the age of 50. We examined 28 children of 13,5 ± 4,9 years. The results showed that 43 % of children suffered hypoxia in utero caused by chronic fetoplacental insufficiency. Up to 64 % of children have abnormalities in the state of their health, causing the risk of thrombosis in the future: hypertension, obesity, cardiac valve disease. 7 children have already suffered thrombosis in vessels of different caliber and localization by the moment of the study. The combination of inherited genes was correlated with the development of thrombosis in children (r = 0,24, p = 0,05).

The article is devoted to the study of activity of lipid peroxidation processes and their influence on oxygen-transport properties of hemoglobin in erythrocytes of peripheral blood of pregnant women with cytomegalovirus infection. Activation of lipid peroxidation processes in pregnant women with cytomegalovirus infection determined by the contents of TBA-active products is accompanied by accumulation of cytotoxic factors - arachidonic acid (1 trimester - up by 31 %, 11 trimester - up by 68 %, 111 trimester - up by 41 %) and lysophosphatidylcholine (1 trimester - up by 85 %, 11 trimester - up by 55 %, 111 trimester - up by 13 %), causes disorders of oxygen-transport properties of hemoglobin in erythrocytes (decrease of total erythrocytes and hemoglobin count), more than double increase of indices of thermolabile hemoglobin at slight decrease of oxyhemoglobin, increase of methemoglobin (1 trimester - up by 60 %, 11 trimester - up by 25 %, 111 trimester - up by 16 %, in comparison with control), that causes decrease of transportation and delivery of oxygen to the tissues and can be the reason of hypoxia.

The article presents the study of superoxide dismutase 2 gene polymorphism that plays an important role in antioxidant protection of an organism. We performed genetic typing in representatives of two ethnic groups of Eastern Siberia - Buryats (Mongoloid) and Russians (Caucasoid). Alleles frequencies among Russians were Ala = 0,492; Val = 0,508; among Buryats Ala = 0,343; Val = 0,657. We compared these frequencies between the populations from Russia and from abroad (according to the results of other researches).

The article presents the results of genetic typing of blood samples of children with glomerulonephritis and congenital abnormalities of the urinary system with different levels of blood pressure by A1166C-polymorphism of gene AGTR1 by the method of polymerase chain reaction. Comparative frequency analysis of genotypes (χ² = 3,74; df = 5; p = 0,39) and alleles degrees of incidence (χ² = 4,28; df = 5; p = 0,31) did not reveal statistically significant differences. Consequently, association of this polymorphism with secondary hypertension wasn't detected.

Comparative analysis of changes of indices of mineral density of bone tissue in patients with chronic traumatic osteomyelitis with different terms of shin synostosis, with united and ununited fractures was performed. Dependence of synostosis terms on the degree of decrease of mineral density of bone tissue on injured extremity was determined. Basing on the study of system and regional indices of mineral density on bone tissue we developed methods of prediction of bone tissue regeneration that allow to reveal disorder in the regeneration process, determine approximate terms of synostosis and estimate effectiveness of the treatment.

The objective of the work was to reveal the peculiarities of damage of the hearing organ at mine explosive trauma and to evaluate the effectiveness of the complex of the procedures during a long period of time. The testing of hearing was conducted in miners who suffered from explosions of methane-air mix on Raspadskaya mine in Mezhdurechensk of Kemerovo region. During the primary examination sensorineural hearing loss of various severity, post-traumatic otitis with rupture of tympanic membrane, unilateral deafness, thermal burns of ear auricles were revealed. All the injured had complex treatment including anti-neuritis therapy, and local anti-inflammatory treatment if necessary. Comparison group included miners who didn't significantly differ by age, working conditions and length of service. Traumatic injuries of the organ of hearing in miners after explosion of methane-air mixture at underground workings occur in 75 ± 3,6 % of cases. During the acute period many injured miners may not be serious enough about the auditory dysfunction on the background of another more expressed pathology. Early audiologic examination of injured miners with observance of all the rules, especially the rules of disguise allows to reveal acute posttraumatic sensorineural hearing loss and deafness. Therapy of hearing loss and middle ear pathology should begin as early as possible to achieve positive firm effect in improving and stabilizing of the hearing. Long-term dynamic monitoring, control of hearing, antineuritis therapy with an individual approach should be continued even after stabilization of the level of hearing loss.

The aim of the research was to optimize the existing methods of surgical treatment of chronic laryngitis, obligate and facultative pre-malignant conditions of the larynx. Surgical treatment of patients with chronic laryngitis, obligate and facultative pre-malignant conditions of the larynx treated in the department of otolaryngology of Municipal Clinical Hospital N 1 of Novokuznetsk and in the department of otolaryngology of Regional Clinical Hospital of War Veterans of Kemerovo during 2009-2012 was conducted. Patients of thefirstgroup had endolaryngeal surgery with direct microlaryngoscopy using surgical microscope and microsurgical tools, patients of the second groups - with indirect laryngoscopy. Assessment of the results of surgical treatment was carried out on the basis of the re-examination of patients in one year (surveys, examination and fibrolaryngoscopy). It was established that use of direct microlaryngoscopy with surgical microscope and microsurgical tools with staining of changed parts of larynx mucous membrane allowed to achieve better visualization when conducting surgical treatment and as a result to reduce the percentage of patients with the development cancerous process in the larynx significantly. Comparison of treatment outcomes in two groups with and without use of microsurgical technique confirms the reliability of this conclusion.

The article presents theoretical ground of the method of correction of intestinal dysbiosis based on a complex immuno-microbiological examination combining research of quantitative and qualitative composition of intestinal microbiota with study of immune reactivity of an organism to bifidus bacteria. As a part of the task we performed bacteriological and serological analysis of 314 coprofiltrate samples of 1-14 years old children living in Irkutsk. On the basis of the studied materials we presented the algorithm of complex study with the ground of possible ways of correction of dysbiotic disorders.

The article presents the description of the clinical follow-up of two children of 4 and 6 years with Kawasaki disease who along with cardiac complications had symptoms of the nervous system damage in the form of the syndrome of narcolepsy and meningoencephalitis. While conducting MRI-angiography of the brain of patient with the syndrome ofnarcolepsy the signs of vasculitis of siphon internal carotid arteries on both sides were discovered. While conducting MSCT of the brain of the child with the meningoencephalitis syndrome the signs of vascular encephalopathy were found. A significant regression of neurological symptoms was observed during the treatment in both children. These cases are interesting because the symptoms of the nervous system damage along with cardiac complications were significantly expressed and the diagnosis of cerebral vasculitis was confirmed by neuroimaging. Cases of the nervous system damage at Kawasaki disease are rare, the literature has little data about the neurological syndromes in this disease, that requires further research in this direction.

The article presents the results of detection of genetic determinants of pathogenicity in 44 strains of Klebsiella (Klebsiella pneumoniae, Klebsiella oxytoca), isolated from the intestinal habitat of infants with intestinal dysbiosis. The presence of the uge gene was detected in 92 % of cases in K. oxytoca and in 90 % of K. pneumoniae. Kfu gene was detected twice as frequent (30 %) in K. pneumoniae strains than in K. oxytoca (12,5 %); bfp gene was detected in K. oxytoca 5 times more frequently (25 %) than in K. pneumoniae (5 %), as well as stx 1 - 29,2 % and 15 %, correspondingly. The presence of stx 2 gene wasn't recorded in any of the DNA samples. Registration of investigated determinants in DNA of Klebsiella spp. autostrains which are non-clinical isolates indicates pathogenicity factors circulation among them and therefore the risk of the formation of intestinal dysbiosis in children.

MIRU-VNTR genetic polymorphism of 156 strains of M. tuberculosis was studied. On the background of significant presence of Beijing genotype strains (44/57 (77,2 %)) we discovered prevalence of epidemically significant Beijing subtype MIT17 (29/44 (65,9 %)) in patients from Mongolia.

69 adolescents of 14-17 years with essential arterial hypertension (EAH) were examined. The most informative indices of lipid peroxidation and antioxidant defense (LPO - AOD) in the blood of adolescents were studied. The state of LPO - AOD system was assessed by determining diene conjugates, ketodienes and conjugated trienes concentrations, superoxide dismutase activity and total antioxidant activity. A method for prediction of the risk of essential hypertension in adolescents was proposed using multivariate discriminant analysis.

The article describes the results of recognaissance surveys of blood serums of humans in Baikal area for hantavirus antibodies and of tissue specimens (lungs) of small mammal for hantavirus antigens. This investigation demonstrated that Baikal area inhabitants contact such dangerous pathogens as hantaviruses. It's proved by the presence of hantavirus antibodies in human serum samples. IgG or IgM antibodies to hantaviruses were found in 3,7 % of 379 tested serum samples. IgM were found in 1,6 % of cases, IgG - in 2,1 % of cases. 2 patients with IgG and IgM with suspected traces of hantavirus infection had complaints to hyperthermia (up to 40,5 °C) and wasting. Blood serums with positive results of hantavirus antibodies were found in residents of Irkutsk region. Hantavirus antigens weren't found in researched lung samples of mouse-like rodent and insectivores from Angarsk, Irkutsk, Zalari and Ust-Kut districts of Irkutsk region and Tunkinsky district of Republic of Buryatia.

The plague natural foci of Kazakhstan are a complicated system of relations between the plague microbe, warm-blood host and vector. The complex approaches used for study of the processes of the plague epizooty and prognosis it. Use of computerized programs with the classic methods of study helps to process the data in time and space. For epidemiological and epizootological analysis the passport data of the strains Y. pestis isolated by Zhosaly Anti-plague Station of the Republic of Kazakhstan in 1990-2012 were processed. The Anti-plague Station carries out the epizootic study of the landscape and epizootology regions as the North Qizilqum, East Karakum, and West part of Aryskum-Dariyalyktakyr. From 1990 to 2012 there were 295 plague strains isolated, 57 % of them were isolated from rodents, and 49,3 % of them were isolated from vectors (ticks and fleas). The passport data for 1990-2011 of three mentioned above landscape and epizootology regions were processed by STATA 12 program; and the regression models of the North Qizilqum and East Karakum were created. The model shows that the plague epizooty activity has been declining by the years. The second model shows that the plague activity in the North Qizilqum has been increasing by the years. Also the passport data of the strains Y. pestis isolated in two natural plague foci of Central Asia desert plague focus as Qizilqum and Aryskum-Dariyalyktakyr natural foci were processed by ArcGIS 10. The regression analysis and modeling in ArcGIS 10 give the opportunities for carrying out of spatial and temporal characteristics of conditions of epizootic activities of the natural plague foci, for detection more possible regions where people livestock as camels can be infected with plague and for prevention of infection of people during their activities. Application of this analysis in the complex of epizootic and epidemiological analysis gives the possibility to prognosis of possible plague epidemic complications and make remote prognosis for carrying out of the prophylaxis measurements under the influences of anthropogenic transformation of the foci, social and climatic factors and can help to survey the foci and protect the population who live there. Use of the regression analysis in the epidemiological and epizootological monitoring of the plague in the natural plague foci can help to prognosis the dynamics of the plague epizooty in the foci and detect a correlation between the microbe, vector, and host of the plague.

The article presents main results of collective researches of Scientific Center of Reconstructive and Restorative Surgery SB RAMS and A.E. Favorsky Irkutsk Institute of Chemistry SB RAS. Introduction of obtained results in clinical practice produces good effect and allows to hope for further collaboration.

The review describes current understanding of the problem of essential hypertension from a perspective of sleep medicine. Sleep disorders in adolescents can be considered as one of constant symptoms of essential hypertension, which are found not only on initial (functional) stages of this disease and but also in the course of the illness. It is now known that about 90 % of sleep disorders are represented by different types of such respiratory disturbances as obstructive sleep-disordered breathing (obstructive sleep apnea/hypopnea syndrome). Considering close interaction between hemodynamic and respiratory systems we can assume possible participation of sleep-disordered breathing in the development of essential hypertension in adolescents.

The review presents current researches of peculiarities of hormonal status, lipid and carbohydrate metabolism of indigenous and migrant populations of the North and Siberia. It is known that extreme climatic and geographical conditions are more demanding to the metabolic status. Features of protein-lipid and carbohydrate metabolism in the North and Siberia are determined by biologically built adaptive type and are connected with adherence of indigenous population to traditional lifestyle and "Asian" type of food. However in the last two decades the decline of quality of health of ethnic groups is registered due to a number of social reasons. Changing lifestyles and food ration causes the development of dysadaptive reactions in the whole organism and the subsequent development of diseases. Numerous studies on metabolic changes in migrant population depending on the length of stay in the northern territories, latitude, seasons, age and gender are conducted.

The article summarizes and analyzes literature data concerning significant degradation of basic indicators of reproductive function of teenage boys and of men of reproductive age. Sharp decrease of fertility and sexual activity of young and middle-aged men is registered in industrialized countries including Russia in recent decades. The results of researches of infertility causes among fertility-challenged couples shows that "male factor" accounts for about a half of the cases. Data about progressing tendency of degradation of children's and adolescents' reproductive health causes particular concern. The diseases of child and adolescent gestation period cause almost a half of the cases of male infertility. The reason of it is the complex of negative factors influencing the population, including growth of stress-producing social factors, sharp aggravation of the ecological situation, increase of quantity of impaired development cases, inadequate use of medicines, alcoholism, smoking, drug addiction and many other things. This situation is considered to be critical, its significance causes carrying out complex researches of reproductive potential of the man population of Russia.

The review of scientific literature describes modern understanding of the problem of teratogenic effect of alcohol on the fetus and pregnancy outcome. The attention is paid to the problems of incidence, the role of short-term intervention in the prevention of fetal alcohol syndrome (FAS) and fetal alcohol spectrum disorders (FASD). It is shown that in Russia accurate epidemiological data on the incidence of fetal alcohol syndrome and fetal alcohol spectrum disorders are not available. It's noted that alcohol consumption in women can lead to adverse pregnancy outcomes. In a review of domestic and foreign literature it was revealed that in Russia there is a lack of information on violations arising in connection with the use of alcohol by child's mother during the prenatal period. FAS and FASD problem are little studied, including Irkutsk region. It was established that there was no data on the prevalence and incidence of FAS and FASD. Prevention issues are covered a bit and are used in physicians' practice not in all regions. Therefore, the study of this problem will be of great theoretical and practical significance.

Treatment of cholelithiasis in patients with high operative risk need requires minimally invasive methods. Contact bilestones litholysis is a perspective direction. For this purpose it is recommended to use medications with the highest litholytic activity and the lowest side effects. It is necessary to seek for an adequate concrement dissolvent because of mineral and organic composition of bilestones. The article presents comparative analysis of characteristics of various dissolving agents proceeding from effectiveness and intensity toxic effects.

The aim of this review is to analyze the literature about the functional state of lipid peroxidation - antioxidant protection system in menopausal women. The activity of free-radical oxidation of lipids in women with sex steroid deficiency depends on the version of its development. The activity of free-radical oxidation of lipids is within age normal range at physiological menopause but there is oxidative stress at pathological climax course that can be determined by genes. The most of works demonstrated deficiency of glutathione, melatonin and reduced glutathione peroxidase activity in menopausal women but data about the content of vitamins are very ambiguous and prove their connection with various pathologies. It should be noted that oxidative stress in perimenopausal women is regarded as a prognostic index of the severity of menopausal symptoms.

The article presents the analysis of the inventive work of the Institute of Traumatology and Orthopaedics being a part of the Scientific Center of Reconstructive and Restorative Surgery SB RAMS. Main directions of patent work including hip replacement technologies, diagnostics and treatment of spine and spinal cord pathologies, methods of managing bone tissue regeneration and of using autologous tissues for osteoplasty, septic osteology, anesthetic management techniques in orthopedic surgery and also of evaluation and modulation of functional state of organism were marked. The effectiveness of patented technologies is proved in the current researches of the Center that allows to transmit them into practical health care.