THE DETECTION OF EPIDEMIC SUBTYPES OF BEIJING GENOTYPE OF MYCOBACTERIUM TUBERCULOSIS CIRCULATED IN THE PRIMORSKY KRAI
https://doi.org/10.29413/ABS.2018-3.5.23
Abstract
Background. The Far East is the territory with high rate of incidence and prevalence of tuberculosis. Cases of tuberculosis caused by epidemic strains have high frequency of MDR and XDR. It is important to study the prevalence of TB in areas with a high burden of infection, to which the Far East belongs. The aim of the research is to carry out genotyping of strains and assess the prevalence of CC1 and CC2 subtypes in the territory of Primorsky Krai.
Materials and methods. The DNAs of 99 clinical isolates of MBT from Primorsky Krai have been genotyped by the 24-locus MIRU-VNTR and RD105/RD207.
Results. The dominant number of strains pertained to Beijing genotype (59.6 %). The express method revealed 22 isolates of the CC2/W148 subtype, which had 6 different MIRU-VNTR-24 profile. According to MLVA classification MtbC 15-9, the most common among the isolates of CC2/W148 profile is 100-32 (59.1 %). Among these profiles the highest frequency of MDR/XDR was recorded – 69,2 %. According to the results of the express analysis, 39 isolates with 26 different MIRU-VNTR-24 profiles belonged to the CC1 subtype, of which the dominant number belonged to 99-32 and 94-32.
Conclusions. The methods of express genotyping of epidemic subtypes of the Beijing genotype are very important for epidemiological surveillance and clinical practice. The developed methods allow to define a wider range of strains than previously used methods.
About the Authors
P. A. Khromova
Scientific Centre for Family Health and Human Reproduction Problems.
Russian Federation
Russian Federation
ul. Timiryazeva 16, Irkutsk 664003.
Junior Research Officer at the Laboratory of Epidemiologically and Socially Important Infections.
M. S. Kornilov
Pacific State Medical University of the Ministry of Health of the Russian Federation.
Russian Federation
Russian Federation
prosp. Ostryakova 2, Vladivostok 690002.
Postgraduate at the Department of Epidemiology and Military Epidemiology.
S. N. Zhdanova
Scientific Centre for Family Health and Human Reproduction Problems.
Russian Federation
Russian Federation
ul. Timiryazeva 16, Irkutsk 664003.
Сandidate of Medical Sciences, Senior Research Officer at the Laboratory of Epidemiologically and Socially Important Infections.
A. A. Yakovlev
Pacific State Medical University of the Ministry of Health of the Russian Federation.
Russian Federation
Russian Federation
prosp. Ostryakova 2, Vladivostok 690002.
Doctor of Medical Sciences, Professor, Professor of the Department of Epidemiology and Military Epidemiology.
O. B. Ogarkov
Scientific Centre for Family Health and Human Reproduction Problems; Irkutsk State Medical Academy of Postgraduate Education – Branch Campus of the Russian Medical Academy of Continuing Professional Education.
Russian Federation
Russian Federation
ul. Timiryazeva 16, Irkutsk 664003.
Yubileynyi 100, Irkutsk 664049.
Doctor of Medical Sciences, Senior Research Officer at the Central Research Laboratory; Head of the Laboratory of Epidemiologically and Socially Significant Infections.
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Review
For citations:
Khromova P.A., Kornilov M.S., Zhdanova S.N., Yakovlev A.A., Ogarkov O.B. THE DETECTION OF EPIDEMIC SUBTYPES OF BEIJING GENOTYPE OF MYCOBACTERIUM TUBERCULOSIS CIRCULATED IN THE PRIMORSKY KRAI. Acta Biomedica Scientifica. 2018;3(5):154-158. (In Russ.) https://doi.org/10.29413/ABS.2018-3.5.23
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