MicroRNA in refined diagnosis of choroidal melanoma
https://doi.org/10.29413/ABS.2021-6.6-1.8
Abstract
Epigenetic studies of the level of microRNAs in human oncogenesis indicate their signifi cant role in the development and growth of malignant tumors of various origins. The fi rst works on the role of microRNAs in patients with uveal melanoma appeared in 2008.
The aim: to analyze the expression level of miRNA-126 and miRNA-223 in the plasma blood of patients and to determine their signifi cance in the refi ned diagnosis of choroidal melanoma.
Materials and methods. We examined 84 patients with choroidal melanoma (CM), mean age – 63.4 ± 1.2 (35–86 y.o.). Localization – a single CM node with a thickness of 0.77–17.19 mm. The control group consisted of 28 volunteers, age – 62.9 ± 1.42 (45–78 y.o.). Plasma miRNA expression levels were determined by real-time PCR.
Results. An increase in the level of expression of miRNA-223 and miRNA-126 in blood plasma was confi rmed in all 84 patients with choroidal melanoma N0M0 compared with the control group. An increase in the expression of miRNA-223 and miRNA-126 was proved with an increase in tumor prominence.
Conclusion. The obtained results of an increase in the expression of miRNA-223 indicate an increase in cell proliferation, and an increase in the expression of miRNA-126 on the activation of angiogenesis in a growing tumor, which makes it possible to recommend a study of the level of miRNA-223 and miRNA-126 for a more accurate diagnosis of small CM in cases of difficulty of differential diagnosis with other tumor-like diseases of the choroid.
About the Authors
A. F. Brovkina
Russian Medical Academy of Continuous Professional Education;
Moscow Ophthalmological Centre, Botkin Hospital
Russian Federation
Russian Federation
Dr. Sc. (Med.), Professor, Academician of RAS, Professor at the Department of Ophthalmology; Ophthalmologist at the Oncology Department
Barrikadnaya str. 2/1, Moscow 123995, Russian Federation
2nd Botkinsky dr. 5 build 19, Moscow 125284, Russian Federation
N. D. Tsybikova
Russian Medical Academy of Continuous Professional Education;
Moscow Ophthalmological Centre, Botkin Hospital
Russian Federation
Russian Federation
Postgraduate at the Department of Ophthalmology; Ophthalmologist at the Polyclinic Department No. 2
Barrikadnaya str. 2/1, Moscow 123995, Russian Federation
2nd Botkinsky dr. 5 build 19, Moscow 125284, Russian Federation
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Review
For citations:
Brovkina A.F., Tsybikova N.D. MicroRNA in refined diagnosis of choroidal melanoma. Acta Biomedica Scientifica. 2021;6(6-1):65-73. (In Russ.) https://doi.org/10.29413/ABS.2021-6.6-1.8
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