actabiomedicaActa Biomedica ScientificaActa Biomedica Scientifica2541-94202587-9596Scientific Centre for Family Health and Human Reproduction Problems10.29413/ABS.2021-6.6-1.8actabiomedica-3112Research ArticleОФТАЛЬМОЛОГИЯOPHTHALMOLOGYМикроРНК в уточнённой диагностике меланомы хориоидеиMicroRNA in refined diagnosis of choroidal melanomahttps://orcid.org/0000-0001-6870-1952БровкинаА. Ф.BrovkinaA. F.

 доктор медицинских наук, профессор, академик РАН, Заслуженный деятель науки РФ, профессор кафедры офтальмологии; врач-офтальмолог онкологического отделения

125993, г. Москва, ул. Баррикадная, 2/1, Россия

125284, г. Москва, 2-й Боткинский пр-д, 5, корп. 19, Россия

 Dr. Sc. (Med.), Professor, Academician of RAS, Professor at the Department of Ophthalmology; Ophthalmologist at the Oncology Department

Barrikadnaya str. 2/1, Moscow 123995, Russian Federation 

2nd Botkinsky dr. 5 build 19, Moscow 125284, Russian Federation

anab@list.ruhttps://orcid.org/0000-0003-2525-3082ЦыбиковаН. Д.TsybikovaN. D.

 аспирант кафедры офтальмологии; врач-офтальмолог поликлинического отделения № 2

125993, г. Москва, ул. Баррикадная, 2/1, Россия

125284, г. Москва, 2-й Боткинский пр-д, 5, корп. 19, Россия

Postgraduate at the Department of Ophthalmology; Ophthalmologist at the Polyclinic Department No. 2 

Barrikadnaya str. 2/1, Moscow 123995, Russian Federation 

2nd Botkinsky dr. 5 build 19, Moscow 125284, Russian Federation

natashatd@bk.ruФГБОУ ДПО «Российская медицинская академия непрерывного профессионального образования» Минздрава России; Московский городской офтальмологический центр ГБУЗ ГКБ им. С.П. Боткина ДЗМRussian Medical Academy of Continuous Professional Education; Moscow Ophthalmological Centre, Botkin Hospital 20212812202166-16573Copyright © Бровкина А.Ф., Цыбикова Н.Д., 20212021Бровкина А.Ф., Цыбикова Н.Д.Brovkina A.F., Tsybikova N.D.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.actabiomedica.ru/jour/article/view/3112

Эпигенетические исследования уровня микроРНК в онкогенезе человека показывают, что они принимают участие практически во всех клеточных процессах, в том числе в развитии и росте опухолей разного генеза. Первые работы о роли микроРНК у больных увеальной меланомой появились в 2008 г.Цель работы: проанализировать уровень экспрессии микро-126 и микро-223 в плазме крови больных и определить их значимость в уточнённой диагностике меланомы хориоидеи.Материалы и методы исследования. Обследовано 84 больных монолатеральной меланомой хориоидеи (МХ), средний возраст – 63,4 ± 1,2 года (35–86 лет), проминенция МХ – 0,77–17,19 мм. Контрольную группу составили 28 волонтёров в возрасте 62,9 ± 1,42 года (45–78 лет). Уровни экспрессии микроРНК в плазме крови определяли методом ПЦР в режиме реального времени.Результаты. Подтверждено увеличение уровня экспрессии микроРНК-223 и микроРНК-126 в плазме крови у всех 84 больных МХ N0M0 (сравнение с контрольной группой). Доказано увеличение экспрессии микроРНК-223 и микроРНК-126, коррелирующихся с проминенцией опухоли.Заключение. Полученные результаты повышения экспрессии микроРНК-223 свидетельствуют не только об усилении клеточной пролиферации, но и об активизации опухолевого ангиогенеза по увеличению экспрессии микроРНК-126. Сказанное позволяет рекомендовать исследование уровня микроРНК-223 и микроРНК-126 для уточнённой диагностики начальных МХ в случаях сложности дифференциальной диагностики с другими опухолеподобными заболеваниями хориоидеи и предопределения возможности существования скрытых метастазов.

Epigenetic studies of the level of microRNAs in human oncogenesis indicate their signifi cant role in the development and growth of malignant tumors of various origins. The fi rst works on the role of microRNAs in patients with uveal melanoma appeared in 2008.The aim: to analyze the expression level of miRNA-126 and miRNA-223 in the plasma blood of patients and to determine their signifi cance in the refi ned diagnosis of choroidal melanoma. Materials and methods. We examined 84 patients with choroidal melanoma (CM), mean age – 63.4 ± 1.2 (35–86 y.o.). Localization – a single CM node with a thickness of 0.77–17.19 mm. The control group consisted of 28 volunteers, age – 62.9 ± 1.42 (45–78 y.o.). Plasma miRNA expression levels were determined by real-time PCR.Results. An increase in the level of expression of miRNA-223 and miRNA-126 in blood plasma was confi rmed in all 84 patients with choroidal melanoma N0M0 compared with the control group. An increase in the expression of miRNA-223 and miRNA-126 was proved with an increase in tumor prominence.Conclusion. The obtained results of an increase in the expression of miRNA-223 indicate an increase in cell proliferation, and an increase in the expression of miRNA-126 on the activation of angiogenesis in a growing tumor, which makes it possible to recommend a study of the level of miRNA-223 and miRNA-126 for a more accurate diagnosis of small CM in cases of difficulty of differential diagnosis with other tumor-like diseases of the choroid.

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The authors declare that there are no conflicts of interest present.