Formation of sustained antitumor effect in mice’s liver and spleen (CBA х C57BL/6)F1 after a single intravenous injection of zymosan-treated neutrophils
https://doi.org/10.12737/23403
Abstract
Application of zymosan in medicine is limited because of its side effects, since the native granules of zymosan directly activate serum complement components and phagocytic cells. In this regard, it was decided at the outset (ex vivo) to incorporate the granules of zymosan in the neutrophils, and only then inject these "loaded" zymosan neutrophils into the bloodstream. Our experiments showed a total inhibition of the weight accretion of both tumor affected liver (from 5220 ± 963 to 1327 ± 219 mg) and spleen (from 328 ± 29 to 187 ± 64 mg) of mice. Thus these data showed that even on the 17th day after a single intravenous injection zymosan-treated neutrophils persist expressed manifestations of anti-tumor activity in the liver and spleen, that may be basis for using this method for antimetastatic preventive measures in liver and other localizations.
About the Authors
A. G. Lyubimov
Research Institute of Fundamental and Clinical Immunology
Russian Federation
Russian Federation
G. Y. Lyubimov
Research Institute of Fundamental and Clinical Immunology
Russian Federation
Russian Federation
V. A. Kozlov
Research Institute of Fundamental and Clinical Immunology
Russian Federation
Russian Federation
References
1. Беседнова Н.Н., Иванушко Л.А., Звягинцева Т.Н., Елякова Л.А. Иммунотропные свойства1→3; 1→6-β-D-глюканов // Антибиотики и химиотерапия. - 2000. -Т. 45 (2). - С. 37-44
2. Любимов Г.Ю., Любимов А.Г. Влияние инкорпорированного в аутологичные макрофаги зимозана на метастатический рост меланомы В16 в печени мышей // Российский иммунологический журнал. - 2011. -Т. 5 (14), № 3-4. - С. 309-314
3. Любимов Г.Ю., Любимов А.Г., Меньщикова Е.Б., Козлов В.А. Превентивное внутривенное введение зимозан-обработанных нейтрофилов подавляет рост меланомы B16 в печени и селезёнке мышей // Сибирский научный медицинский журнал. - 2016. - Т. 36, № 2. - С. 45-49
4. Маянский Д.Н., Цырендоржиев Д.Д. Реактивность макрофагов лёгких и печени нормальных и предварительно стимулированных животных // Патологическ. физиол. эксперим. терапия. - 1989. -№ 4. - С. 44-48
5. Маянский Д.Н., Цырендоржиев Д.Д., Йонкер А.М. Индукция гранулематозного воспаления печени неинфекционными частицами // Патологич. физиол. эксперим. терапия. -1990. - № 5. - С. 45-49
6. Akramiene D., Kondrotas A., Didziapetriene J., Kevelaitis E (2007). Effects of ß-glucans on immune system. Medicina (Kaunas), 43 (8), 597-606.
7. Deimann W., Faimi H.D. (1980). Induction of focal Hemopoiesis in adult rat liver by glucan, a macrophage activator. Laboratory Investigation, 42 (2), 217-225.
8. Faradji A., Bohbot A., Schmitt-Goguel M., Roeslin N., Dumont S., Wiesel M.L., Lallot C., Eber M., Bartholevns J., Poindron P., Morand G., Witz J.P., Oberling F. (1991). Phase I trial of intravenous infusion of ex-vivo-activated autologous blood-derived macrophages in patients with non-small-cell lung cancer: toxicity and immunomodulatory effects. Cancer Immunol. Immunother., 33 (5), 319-326.
9. Gregory A.D., Houghton A.M. (2011). Tumor-associated neutrophils: new targets for cancer therapy. Cancer Res., 71 (7), 2411-2416.
10. Houghton A.M. (2010). The paradox of tumor-associated neutrophils: fueling tumor growth with cytotoxic substances. Cell Cycle, 9 (9), 1732-1737.
11. Lala A., Lindemann R.A., Miyasaki K.T. (1992). The differential effects of polymorphonuclear leukocyte secretion on human natural killer cell activity. Oral Microbiol. Immunol., 7 (2), 89-95.
12. Morikawa K., Takeda R., Yamazaki M., Mizuno D. (1985). Induction of tumoricidal activity of polymorphonuclear leukocytes by a linear beta-1,3-D-glucan and other immunomodulators in murine cells. Cancer Res., 45 (4), 1496-1501.
13. Pekarek L.A., Starr B.A., Toledano A.Y., Schreiber H. (1995). Inhibition of tumor growth by elimination of granulocytes. J. Exp. Med., (181), 435-440.
14. Qi C., Cai Y., Gunn L., Ding C., Li B., Kloecker G., Qian K., Vasilakos J., Saijo S., Iwakura Y., Yanelli J.R., Yan Jen J. (2011). Differential pathways regulating innate and adaptive anti-tumor immune responses by particulate and soluble yeast-derived B-glucans. Blood, 117 (25), 6825-6836.
15. Schirmer W.J., Schirmer J.M., Naff G.B., Fry D.E. (1988). Contribution of toxic oxygen intermediates to complement-induced reductions in effective hepatic blood flow. J. Trauma, 28 (9), 1295-1300.
16. Schirmer W.J., Schirmer J.M., Naff G.B., Fry D.E. (1988). Systemic complement activation produces hemodynamic changes characteristic of sepsis. Arch. Surg., 123 (3), 316-321.
17. Smith H.A., Kang Y. (2013). The metastasis-promoting roles of tumor-associated immune cells. J. Mol. Med. (Berl.), 91 (4), 411-429.
18. Suzuki T., Ohno N., Chiba N., Miura N.N., Adachi Y., Yadomae T. (1996). Immunopharmacological activity of the purified insoluble glucan, zymocel, in mice. J. Pharm. Pharmacol., 48 (12), 1243-1248.
19. Tateishi T., Ohno N., Adachi Y., Yadomae T. (1997). Increases in Hematopoietic responses caused by ß-glucans in mice. Biosci. Biotech. Biochem, 61 (9), 1548-1553.
20. Underhill D.M. (2003). Macrophage recognition of zymosan particles. J. Endotoxin Res. (3), 176-180.
21. Volman T.J., Hendriks T., Goris R.J. (2005). Zymosan-induced generalized inflammation: experimental studies into mechanisms leading to multiple organ dysfunction syndrome. Shock, 23 (4), 291-297.
22. Williams D.L., Sherwood E.R., McNamee R.B., Jones E.L., Di Luzio N.R. (1985). Therapeutic efficacy of glucan in a murine model of hepatic metastatic disease. Hepatology, 5 (2), 198-206.
23. Winn A.A., Miyakawa K., Miyata E., Dranoff G., Takeya M., Takahashi K.L. (2001). Role of granulocyte / macrophage colony-stimulating factor in zymocel-induced hepatic granuloma formation. Am. J. Pathol., 158 (1), 131-145.
24. Wooles W.R., Di Luzio N.R. (1964). The phagocytic and proliferative response of the reticuloendothelial system following glucan administration. J. Reticuloendothel. Soc., (1), 160-169.
Review
For citations:
Lyubimov A.G., Lyubimov G.Y., Kozlov V.A. Formation of sustained antitumor effect in mice’s liver and spleen (CBA х C57BL/6)F1 after a single intravenous injection of zymosan-treated neutrophils. Acta Biomedica Scientifica. 2016;1(5):110-113. (In Russ.) https://doi.org/10.12737/23403
Views:
621
.png)
Email this article 
