Lipid Peroxidation in the Pre-Reactive Period of Cold Injury

Pathological processes in the pre-reactive period of cold injury have not been studied.

The purpose of this study is to assess the morphological and biochemical parameters of blood in the body of patients in the pre-reactive period of cold injury.

Material and methods. We examined patients with cold injury in the pre-reactive period. Determined: diene conjugates, malonic dialdehyde, the total content of low molecular weight antioxidants, superoxide dismutase activity, catalase,glutathione peroxidase, glutathione reductase, using a SPECORD 40 spectrophotometer. The serum biochemical parameters in the serum were determined by biochemical automator analysis. Statistical processing of the data was performed using IBM SPSS Statistics 19.

Results and discussion. Features of the biochemical profile of blood in the pre-reactive period of cold injury in the human body are an increase in concentration of glucose and triacylglycerides in blood, an increase in transaminase activity, as aresult of inflammatory and destructive processes, a decrease in the cholesterol concentration in the blood of patients. At the same time, the pre-reactive period of cold injury is characterized by an increase in monocytes compared with the control, which, in combination with hypoxia, leads to an intensification of lipid peroxidation processes. In addition, the antioxidant protection in the pre-reactive period in the body of patients increased slightly.

Conclusion. According to the results of our research, already at the stage of the pre-reactive period of cold injury there is a significant intensification of free radical lipid oxidation processes.

1. Romanova AN, Alekseev RZ, Nikolaev VM, Sofronova SI. Problems of diagnostics and treatment of cold injury in the Arctic zone of the Republic of Sakha (Yakutia). In: Samsonova IV, Neustroeva AI, Malyasheva MM. (eds.). Arkticheskiy vektor: strate-giya razvitiya: Materialy II A82 nauchno-prakticheskoy konferentsii «Arkticheskiy vektor». Yakutsk: Akademiya nauk Respubliki Sakha (Yakutiya); 2019: 56-58. (In Russ.)

2. Alekseev RZ, Tomskiy MI, Golderova AS, Potapov AF, Alekseev YuR, Semenova SV. Prevention of necrosis in frostbites with tissue icing. Mezhdunarodnyy zhurnal prikladnykh i fundamen-tal'nykh issledovaniy. 2015; (8-1): 35-41. (In Russ.)

3. Shapovalov KG. Frostbites in the intensivist's practice. Vestnik anesteziologii i reanimatologii. 2019; 16(1): 63-68. doi: 10.21292/2078-5658-2019-16-1-63-68. (In Russ.)

4. Colburn TD, Ferguson SK, Holdsworth CT, Craig JC, Musch TI, Poole DC. Effect of sodium nitrite on local control of contracting skeletal muscle microvascular oxygen pressure in healthy rats. J Appl Physiol (1985). 2017; 122(1): 153-160. doi: 10.1152/japplphysiol.00367.2016

5. Shakirov BM, Tagaev KR. Our experience of the treatment of local cold injury of the extremity. Vestnik ekstrennoy meditsiny. 2017; X(1): 29-32. (In Russ.)

6. Bhattacharya S. Reactive oxygen species and cellular defense system. In: Rani V, Yadav U. (eds.). Free Radicals in Human Health and Disease. New Delhi: Springer India; 2015. 17-29. doi: 10.1007/978-81-322-2035-0_2

7. Mayer D, Armstrong D, Schultz G, Percival S, Malone M, Romanelli M, et al. Cell salvage in acute and chronic wounds: a potential treatment strategy. Experimental data and early clinical results. J Wound Care. 2018; 27(9): 594-605. doi: 10.12968/jowc.2018.27.9.594

8. Yurtaeva EYu, Dorovskikh VA, Simonova NV, Anokina RA, Shtarberg MA. The effectiveness of natural antioxidants in the adaptation of an organism to cold. Byulleten' fiziologii i patologii dykhaniya. 2017; (63): 70-74. doi: 10.12737/arti-cle_58e453527d0fc8.57228180. (In Russ.)

9. Situnayake RD, Crump BJ, Zezulka AV, Davis M, McConkey B, Thurnham DI. Measurement of conjugated diene lipids by derivative spectroscopy in heptane extracts of plasma. Ann Clin Biochem. 1990; 27(3): 258-266. doi: 10.1177/000456329002700313

10. Nahar Z, Sarwar MS, Safiqul Islam M, Rahman A, Nazrul Islam S, Islam MS, et al. Determination of serum antioxidant vitamins, glutathione and MDA levels in panic disorder patients. Drug Res (Stuttg). 2013; 63(8): 424-428. doi: 10.1055/s-0033-1343494

11. Vartanyan LS, Gurevich SM. Study of the detection of superoxide dismutase activity in animal tissues using tetrani-trotetrazolium blue. Voprosy meditsinskoy khimii. 1982; 28(5): 23-26. (In Russ.)

12. Korolyuk MA, Ivanova LI, Mayorova IG, Tokarev VE. Method of detection of catalase activity. Laboratornoe delo. 1988; (1): 1619. (In Russ.)

13. Danilova LA. Guide on the laboratory tests. Saint Petersburg: Piter; 2003. (In Russ.)