Study of the Effect of a Biologically Active Compound Tris(2-hydroxyethyl)ammonium 4-Chlorophenylsulfanylacetate on the Growth of Listeria monocytogenes and Staphylococcus aureus

Background. Development of nutrient media ensuring the maximum growth rate of pathogens of dangerous infectious diseases while preserving their biological properties is extremely important. A promising direction in this area seems to be the use of synthetic microbial growth biostimulants.

The aim of the work is to study the possibility of improving nutrient media for the cultivation of Listeria and Staphylococcus using a biologically active compound tris(2-hydroxyethyl)ammonium 4-chlorophenylsulfanylacetate.

Materials and methods. The object of the study was experimental nutrient medium for the cultivation of Listeria used for the culturing of the test strain Listeria monocytogenes 766. As a comparison medium, commercial medium Fraser broth to which agar was added at a concentration of 1.5 %, was used. The test strain Staphylococcus aureus ATCC 6538-P (FDA 209-P) was cultivated on meat-peptone agar with 1% glucose. The compound tris(2-hydroxyethyl) ammonium (4-chlorophenyl)sulfanylacetate at a concentration of 10–4 wt. % was studied as a growth stimulator. A nutrient medium without a stimulant served as a control. The specific activity of nutrient media (germination rate, medium sensitivity, growth rate and stability of the main biological properties of microorganisms) was evaluated by the microbiological method.

Results. Studies have shown that the addition of a growth stimulator to nutrient media contributes to the growth of colonies (by 10–50 %) and a decrease in the time of their development. When growth stimulator was added to the nutrient medium for the cultivation of Listeria, the initial growth of colonies of the L. monocytogenes 766 test strain after 12 hours of cultivation and growth of colonies of the test strain S. aureus ATCC 6538-P after 6 hours of cultivation on the meat-peptone agar with 1% glucose was observed.

Conclusion. Thus, the addition of a growth biostimulator tris(2-hydroxyethyl)ammonium 4-chlorophenylsulfanyl acetate at a concentration of 10–4 wt. % in the nutrient medium accelerates the growth of Listeria and Staphylococcus, allows to reduce the time of issuance of the analysis result in half.

1. Ibragimova MA. Modern aspects of listerious infection (literature review). Vestnik Almatinskogo gosudarstvennogo instituta usovershenstvovanija vrachej. 2016; (1): 84-91. (In Russ.)

2. Vallim DC, Hofer CB, Rodrigo de Castro L, Victor BA, Alves RL, Moura FC, et al. Twenty years of listeria in Brazil: occurrence of Listeria species and Listeria monocytogenes serovars in food samples in Brazil between 1990 and 2012. BioMed Research International. 2015; 2015: 540204. doi. 10.1155/2015/540204

3. Gaziumarova LD, Titov LP, Levshina NN, Bogush AA, Strizhak IV, Rogacheva TA, et al. Testing new nutrient media for accumulating and isolating listeria for microbiological monitoring. Meditsina. 2014; (3): 57-61. (In Russ.)

4. Tartakovskij IS. Listeria: role in human infectious pathology and laboratory diagnostics. Clinical Microbiology and Antimicrobial Chemotherapy. 2000; 2(2): 20-30. (In Russ.)

5. Frize K, Kahel V. Infectious diseases of pregnant and newborn. Moscow: Medicine; 2003. (In Russ.)

6. Swaminathan B, Gerner-Smidt P. The epidemiology of human listeriosis. Microbes and Infection. 2007; 9(10): 1236-1243. doi: 10.1016/j.micinf.2007.05.011

7. Nichols RL. Preventing surgical site infections. Clinical Medicine, Research. 2004; 2(2): 115-118. doi: 10.3121/cmr.2.2.115

8. Labinskaia AS, Kostyukova NN (eds). Manual of Medical Microbiology. Moscow: BINOM; 2013. (In Russ.)

9. Mirskova AN, Adamovich SN, Mirskov RG, Voronkov MG. Pharmacologically active salts and ionic liquids based on 2-hydroxyethylamines, arylchalcogenylacetic acids, and essential metals. Russian Chemical Bulletin. 2014; 63(9): 1869-1883. doi: 10.1007/s11172-014-0679-3

10. Adamovich SN. New atranes and similar ionic complexes. Synthesis, structure, properties. Appl Organomet Chem. 2019; 33(7): e4940. doi: 10.1002/aoc.4940

11. Mirskova AN, Adamovich SN, Vinogradov ЕYa, Mirskov RG. Meningococcus growth stimulators for meningitis diagnostics based on 2-hydroxylalkylamine salts. Bulletin of the East Siberian Scientific Center SB RAMS. 2012; (5-1): 276-280. (In Russ.)

12. Polyak MS, Sukharevich VI, Sukharevich ME. Growth media for medical microbiology. St. Petersburg; 2003. (In Russ.)

13. Khaptanova NM, Andreevskaya NM, Lukyanova SV, Kuznetsov VI, Konovalova JA, Mikhailova VA, et al. Study of the physicochemical and biological properties of the cultural medium for the cultivation of listerias. Aktual’nye problemy bolezney, obshchikh dlya cheloveka i zhivotnykh. Materialy III Vserossiyskoy nauchno-prakticheskoy konferentsii, Stavropol’, 24-25 aprelya 2019 g. Stavropol; 2019: 291-292. (In Russ.)