Bacteria Biofilms in Purulent-Septic Infections
https://doi.org/10.29413/ABS.2019-4.5.6
Abstract
The causative agents of many infectious diseases can exist in the form of biofilms. The aim of the work is to study of the frequency of occurrence and the degree of activity of biofilm formation of microorganisms isolated from different locus in purulent-septic infections.
Materials and methods. Fifteen strains isolated from patients with purulent-septic infections were examined. Biofilms were determined by the ability to adsorption a crystalviolet to ethanol.
Results. 73,3 ± 11,4 % strains had biofilms (including gram-negative bacteria – 69,2 ± 11,9 %; Staphylococcus – 100,0 %; p < 0,05).The degree of activity of formation of biofilm by gram-negative bacteria was higher than Staphylococcus (0,302 ± 0,04 и 0,134 ± 0,01 units of optical density; p < 0,01). The highest activity of formation of biofilm was detected in K. pneumoniae isolated from patients with sepsis. Strains from clinically important locus (blood, sputum, wound discharge, abdominal fluid) had biofilms in 75,0 %; from locus of monitoring – 66,7 %. The pathogens isolated from locus of the monitoring were characterized by an average degree of activity of biofilm formation (0,180–0,360 units of optical density). Strains from clinically important locus (blood and sputum from patients with sepsis) had a high
degree of biofilm formation (more than 0,360 units of optical density).
Conclusion. In most cases, strains were characterized by the presence of biofilms and differed in degrees activity of biofilm formation depending on locus.
About the Authors
E. D. Savilov
Irkutsk State Medical Academy of Continuing Education – Branch of the Russian Medical Academy of Continuing Professional Education,
Scientific Centre for Family Health and Human Reproduction Problems
Russian Federation
Russian Federation
Dr. Sc. (Med.), Professor, Head of the Department of Epidemiology and Microbiology; Chief Research Officer at the Laboratory of Epidemiologically and Socially Important Infections
Timiryazev str. 16, 664003 Irkutsk, Russian Federation
Yubileyniy 100, 664049 Irkutsk, Russian Federation
E. V. Anganova
Scientific Centre for Family Health and Human Reproduction Problems
Russian Federation
Russian Federation
Dr. Sc. (Biol.), Laboratory of Epidemiologically and Socially Important Infections
O. A. Noskova
Irkutsk State Regional Children’s Clinical Hospital; Scientific Centre for Family Health and Human Reproduction Problems
Russian Federation
Russian Federation
Deputy Chief Physician for Sanitary and Epidemiological Work; Junior Research Officer at the Laboratory of Epidemiologically and Socially Important Infections
Timiryazev str. 16, 664003 Irkutsk, Russian Federation
Gagarina Blvd. 4, 664022 Irkutsk, Russian Federation
A. V. Dukhanina
Scientific Centre for Family Health and Human Reproduction Problems
Russian Federation
Russian Federation
Cand. Sc. (Biol.), Professor, Engineer at the Laboratory of Epidemiologically and Socially Important Infections
Timiryazev str. 16, 664003 Irkutsk, Russian Federation
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Review
For citations:
Savilov E.D., Anganova E.V., Noskova O.A., Dukhanina A.V. Bacteria Biofilms in Purulent-Septic Infections. Acta Biomedica Scientifica. 2019;4(5):38-42. (In Russ.) https://doi.org/10.29413/ABS.2019-4.5.6
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