IDENTIFICATION OF THEILERIA EQUI IN HORSE BLOOD IN THE IRKUTSK REGION
Abstract
This paper describes the detection of Piroplasmida DNA in the blood of horses. 166 blood samples of horses from five districts of the Irkutsk region were studied. The high piroplasms infection rate in horses (69 %) was found. For six positive samples nucleotide sequences were determined. The comparison of determined 18S rRNA gene sequences with previously published sequences showed that all revealed piroplasms DNA samples are Theileria equi. The data are presented on the lethal cases of equine piroplasmosis in the Irkutsk region in 2008.
About the Authors
O. O. Fedulina
Scientific Center for Family Health and Human Reproduction Problems
Russian Federation
Russian Federation
O. V. Suntsova
Scientific Center for Family Health and Human Reproduction Problems
Russian Federation
Russian Federation
V. A. Rar
Institute of Chemical Biology and Fundamental Medicine SB RAS
Russian Federation
Russian Federation
I. V. Meltsov
Irkutsk State Agricultural Academy; Veterinary Service of the Irkutsk region
Russian Federation
Russian Federation
I. V. Kozlova
Scientific Center for Family Health and Human Reproduction Problems
Russian Federation
Russian Federation
O. V. Lisak
Scientific Center for Family Health and Human Reproduction Problems
Russian Federation
Russian Federation
V. V. Chekushkina
ZAO Irkut
Russian Federation
Russian Federation
References
1. Федулина О.О., Рар В.А., Сунцова О.В., Козлова И.В. Результаты рекогносцировочных исследований по обнаружению очагов бабезиоза на территории Иркутской области // Бюллетень ВСНЦ СО РАМН, 2013. - № 2-2 (90). - Р. 130-133
2. Adaszek L., Gorna M., Krzysiak M., Garbal M., Winiarczyk S. Identification of the piroplasms isolated from horses with clinical piroplasmosis in Poland // Wiad. Parazytol. - 2011. - Vol. 57 (1). - P. 21-26.
3. Equine piroplasmosis. - http://www.cfsph.iastate. edu.
4. Hall C.M., Busch J.D., Scoles G.A., Palma-Cagle K.A., Ueti M.W., Kappmeyer L.S., Wagner D.M. Genetic characterization of Theileria equi infecting horses in North America: evidence for a limited source of U.S. introduction // Parasites & Vectors. - 2013. - Vol. 6. - P. 35.
5. Knowles D.P. Jr., Kappmeyer L.S., Stiller D., Hennager S.G., Perryman L.E. Antibody to a recombinant merozoite protein epitope identifies horses infected with Babesia equi // J. Clin. Microbiol. - 1992. - N 30. - P. 3122-3126.
6. Lazarev V.V. Piroplasmoses of ruminant animals // Veterinary of the Kuban. - 2008. - N 2. - P. 29-31.
7. Qablan M.A., Obornik M., Petrzelkova K.J., Sloboda M., Shudiefat M.F., Horin P., Lukes J., Modry D. Infections by Babesia caballi and Theileria equi in Jordanian equids: epidemiology and genetic diversity // Parasitology, Cambridge University Press. - 2013. - P. 1-8.
8. Rar V.A., Epikhina T.I., Livanova N.N., Panov V.V. Genetic diversity of Babesia in Ixodes persulcatus and small mammals from North Ural and West Siberia, Russia // Parasitology. - 2011. - Vol. 138. - P. 175-182.
9. Salib F.A., Youssef R.R., Said S.F. Eppidemiology, diagnosis and therapy of Theileria equi infection in Giza, Egypt // Vet. World. - 2013. - Vol. 6 (2). - P. 76-82.
10. Seo M.G., Yun S.H., Choi S.K., Cho G.J., Park Y.S., Kwon O.D., Cho K.H., Kim T.H., Jeong K.S., Park S.J., Kwon Y.S., Kwak D. Seroprevalence of equine piroplasms in the Republic of Korea // Vet. Parasitol. - 2012. - Vol. 179 (1-3). - P. 224-226.
11. Sloboda M., Jirku M., Lukesova D., Qablan M., Batsukh Z., Fiala I., Horin P., Modry D., Lukes J. A survey for piroplasmids in horses and Bactrian camels in North-East ern Mongolia // Vet. Parasitol. - 2011. - Vol. 179 (1-3). - P. 246-249.
12. Steinman A., Zimmerman T., Klement E., Lensky I.M., Berlin D., Gottlieb Y., Baneth G. Demographic and environmental risk factors for infection by Theileria equi in 590 horses in Israel // Vet. Parasitol. - 2012. - Vol. 187 (304). - P. 558-562.
13. Stiller D., Goff W.L, Johnson L.W., Knowles D.P. Dermacentor variabilis and Boophilus microplus (Acari: Ixodidae): experimental vectors of babesia equi to equids // J. Med. Entomol. - 2002. - Vol. 39 (4). - P. 667-670.
14. Ueti M.W., Palmer G.H., Kappmeyer L.S., Statdfield M., Scoles G.A., Knowles D.P. Ability of the vector tick Boophilus microplus to acquire and transmit Babaesia equi following feeding on chronically infected horses with low-level parasitemia // J. Clin. Microbiol. - 2005. - Vol. 43 (8). - P. 3755-3759.
Review
For citations:
Fedulina O.O., Suntsova O.V., Rar V.A., Meltsov I.V., Kozlova I.V., Lisak O.V., Chekushkina V.V. IDENTIFICATION OF THEILERIA EQUI IN HORSE BLOOD IN THE IRKUTSK REGION. Acta Biomedica Scientifica. 2014;(6):101-104. (In Russ.)
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