CORRECTION OF INTERFERON-α-INDUCED DENDRITIC CELL DYSFUNCTIONS IN IMMUNOCOMPROMISED PATIENTS
Abstract
The correction of impaired functions of in vitro generated interferon-alpha-induced dendritic cells (IFN-DCs) in patients with malignant brain tumors (n = 8), malignant lymphomas (n = 14) and pulmonary tuberculosis (n = 26) was analyzed in the present study. The principal possibility of restoring reduced functional activity of patient DCs was shown. The addition of recombinant human IL-2, complex of pro-inflammatory cytokines, double-stranded human DNA or polyoxidonium to IFN-DC cultures at the stage of maturation could significantly increase the allostimulatory activity of DCs in patients with malignant lymphomas and pulmonary tuberculosis, as well as enhance the cytotoxic activity of cancer patient DCs against NK- and TRAIL-resistant tumor cell line HEp-2. These findings offer new approaches to the correction of DC dysfunctions in the pathology and justify ways of improving the treatment of infectious diseases and cancer using dendritic cell vaccines.
About the Authors
O. Yu. Leplina
Institute of Clinical Immunology SB RAMS
Russian Federation
Russian Federation
L. V. Sakhno
Institute of Clinical Immunology SB RAMS
Russian Federation
Russian Federation
A. A. Ostanin
Institute of Clinical Immunology SB RAMS
Russian Federation
Russian Federation
S. S. Bogachev
Institute of cytology and genetics SB RAMS
Russian Federation
Russian Federation
E. R. Chernykh
Institute of Clinical Immunology SB RAMS
Russian Federation
Russian Federation
References
1. Леплина О.Ю., Насонова Г.В., Тихонова М.А., Крючкова И.В. и др. Характеристика IFNα -индуцированных дендритных клеток у больных злокачественными лимфомами // Гематология и трансфузиология. - 2008. - № 3. - C. 24-29.
2. Леплина О.Ю., Насонова Г.В., Тихонова М.А., Крючкова И.В. и др. «IFNα-индуцированные дендритные клетки у больных множественной миеломой» // Сибир. онколог. журн. - 2009. - № 6. -С. 37-43.
3. Леплина О.Ю., Тихонова М.А., Борисова А.Е., Старостина Н.М. и др. Фенотип и функции дендритных клеток у больных хроническими вирусными гепатитами // Мед. иммунология. - 2009. - Т. 2/3. - С. 191-196.
4. Сахно Л.В., Леплина О.Ю., Тихонова М.А., Распай Ж.М. и др. Характеристика дендритных клеток, генерируемых в присутствии интерферона-α у больных туберкулезом легких // Проблемы туберкулеза. - 2007. - № 3. - С. 42-46.
5. Carbonneil C., Aouba A., Burgard M. Dendritic cells generated in the presence of GM-CSF and Interferon-alpha are potent inducers of HIV-specific CD8+ T-cells // AIDS. - 2003. - Vol. 17. -P. 1731 -1740.
6. Della Bella S., Nicola S., Brambilla L. Quantitative and functional defects of dendritic cells in classic Kaposi's sarcoma // Clin. Immunol. - 2006. -Vol. 119. - P. 317-329.
7. Leplina O.Yu., Tyrinova T.V., Tikhonova M.A., Shevela E.Ya. et al. Direct antitumor activity of interferon-induced dendritic cells of healthy donors and patients with primary brain tumors / In: Glioma -exploring it's biology and practical relevance; ed. by. A. Ghosh. - 2011. - P. 325-342.
8. Santini S., Lapenta C., Belardelli F. Type I interferons as regulators of the differentiation/activation of human dendritic cells: methods for the evaluation of IFN-induced effects // Meth. Mol Med. - 2005. -Vol. 116. - P. 167-181.
9. Thurner B., Roder C., Dieckmann D. Generation of large numbers of fully mature and stable dendritic cells from leukapheresis products for clinical applications // J. Immunol. Meth. - 1999. - Vol. 223. -P. 1-15.
10. Van der Molen R.G., Sprengers D., Binda R.S. Functional impairment of myeloid and plasmacytoid dendritic cells of patients with chronic hepatitis B // Hepatology. - 2004. - Vol. 40. - P. 738-746.
11. Zhang Z.J., Fu Q., Zhao Y. Differential restoration of myeloid and plasmacytoid dendritic cells in HIV-1-infected children after treatment with highly active antiretroviral therapy // J. Immunol. - 2006. -Vol. 176. - P. 5644-5651.
Review
For citations:
Leplina O.Yu., Sakhno L.V., Ostanin A.A., Bogachev S.S., Chernykh E.R. CORRECTION OF INTERFERON-α-INDUCED DENDRITIC CELL DYSFUNCTIONS IN IMMUNOCOMPROMISED PATIENTS. Acta Biomedica Scientifica. 2012;(3(2)):104-108. (In Russ.)
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