COMPARATIVE ANALYSIS OF CRISPR-CAS SYSTEM STRUCTURES OF YERSINIA PSEUDOTUBERCULOSIS IP32953 AND IP31758
https://doi.org/10.29413/ABS.2018-3.5.8
Abstract
Background. Pseudotuberculosis is still relevant problem in medical science and public health of Russia and other countries. Typing of Y. рseudotuberculosis strains by their CRISPR systems is a perspective tool for monitoring of Yersinia populations as was shown in Y. pestis.
Aims. Here we describe and compare CRISPR-Cas systems of Yersinia pseudotuberculosis strains IP32953 and IP31758 causing classic pseudotuberculosis and Far-East scarlet-like fever (FESLF) respectively.
Materials and methods. Complete genomes of Y. pseudotuberculosis IP329353 and IP31758 (NC_006155 and NC_009708 respectively) were obtained from NCBI Nucleotide Database. Search; identification; and analysis of CRISPR systems were carried out by online-tools CRISPROne; CRISPRDetect; and CRISPRTarget.
Results and discussion. Analyzed strains have CRISPR-Cas systems that include one set of cas-genes and arrays situated at the long distances from each other. We defined three CRISPR arrays in Y. pseudotuberculosis IP32953 by the combination of program methods. CRISPR-Cas system of this strain consist of array YP1 located near cas-genes; arrays YP2 and YP3. CRISPR-Cas system of Y. pseudotuberculosis IP31758 includes two arrays – YP1 and YP3. CRISPR systems do not share similar spacers. CRISPR systems of the analyzed strains differ in CRISPR loci and cas-protein structures that can be used as specific marks of analyzed strains.
Conclusions. We suggest that acquisition of certain spacers may play a role in evolution and divergence of Y. pseudotuberculosis strains.
About the Authors
N. P. Peretolchina
Irkutsk State Medical University; Irkutsk Antiplague Research Institute of Siberia and Far East of Rospotrebnadzor.
Russian Federation
Russian Federation
ul. Krasnogo Vosstaniya 1, Irkutsk 664003.
ul. Trilissera 78, Irkutsk 664047.
Postgraduate at the Department of Microbiology, Virology and Immunology; Clinical Research Assistant.
A. Y. Borisenko
Irkutsk State Medical University.
Russian Federation
Russian Federation
ul. Krasnogo Vosstaniya 1, Irkutsk 664003.
Postgraduate, Teaching Assistant at the Department of Microbiology, Virology and Immunology.
Yu. P. Dzhioev
Irkutsk State Medical University.
Russian Federation
Russian Federation
ul. Krasnogo Vosstaniya 1, Irkutsk 664003.
Candidate of Biological Sciences, Leading Research Officer, Head of the Laboratory of Molecular Virology and Biotechnology, Research Institute of Biomedical Technologies.
V. I. Zlobin
Irkutsk State Medical University.
Russian Federation
Russian Federation
ul. Krasnogo Vosstaniya 1, Irkutsk 664003.
Doctor of Medical Sciences, Professor, Academician of RAS, Head of the Department of Microbiology, Virology and Immunology, Director of the Research Institute of Biomedical Technologies.
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Review
For citations:
Peretolchina N.P., Borisenko A.Y., Dzhioev Yu.P., Zlobin V.I. COMPARATIVE ANALYSIS OF CRISPR-CAS SYSTEM STRUCTURES OF YERSINIA PSEUDOTUBERCULOSIS IP32953 AND IP31758. Acta Biomedica Scientifica. 2018;3(5):54-59. (In Russ.) https://doi.org/10.29413/ABS.2018-3.5.8
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