DETECTION OF CAUSATIVE AGENTS OF TICK-BORNE INFECTIONS BY POLYMERASE CHAIN REACTION ASSAY CARRIED OUT AS A PART OF CLINICAL STUDIES IN THE “REPUBLICAN CLINICAL INFECTIOUS DISEASES HOSPITAL” OF ULAN-UDE

The article is devoted to the study of vector-borne pathogens circulating in the Republic of Buryatia. The authors have carried out  identification of the genetic material of tick-borne encephalitis virus,  Borrellia burgdorferi, Borrellia miуamotoi, Coxiella burnetii and  Rickettsia sibirica in various samples of biological material. The  object of the study were whole blood, leukocyte fraction and blood  serum of patients admitted to the hospital in a state of fever that  occurred after sucking of the tick, as well as mites brought by the  population. A detailed analysis of the data showed that 11.7 % (9  out of 70) of the clinical material samples and 17.8 % (50 out of  281) of the tick samples had genetic markers of tick-borne  pathogens. In the material from the patients, the RNA of tick-borne  encephalitis virus was most often determined, and in the DNA of  Borrellia burgdorferi mites. In addition, the authors first confirmed  the etiologic role of Borrellia miуamotoi in the development of cases  of Lyme disease in the Republic. An unexpected finding was the  detection of Coxiella burnetii DNA in ticks and in clinical material. In  the Republic of Buryatia Q-fever was detected by laboratory  diagnostics for the first time. This fact confirms the circulation of this pathogen in the region. In this regard, the authors conducted a  retrospective analysis of the medical histories of patients who were found to have genetic markers of Q-fever.

1. Багаутдинова Л.И., Сарксян Д.С., Дудадров М.В., Малинин О.В. Клинический полиморфизм заболевания, вызываемого Borrelia miyamotoi // Практическая медицина. – 2013. – № 5. – С. 125–130. Bagautdinova LI, Sarksyan DS, Dudarev MV, Malinin OV. (2013). Clinical polymorphism of disease caused by Borrelia miyamotoi [Klinicheskiy polimorfizm zabolevaniya, vyzyvaemogo Borrelia miyamotoi]. Prakticheskaya meditsina, (5), 125-130.

2. Бондаренко Е.И., Щучинова Л.Д., Тимофеев Д.И., Мишенова Е.В. Выявление генетических маркеров возбудителей клещевых риккетсиозов в ПЦР с помощью наборов реагентов «РеалБест ДНК Rickettsiaspecies» и «РеалБест ДНК Rickettsia sibirica/Rickettsia heilongjiangensis» // Новости «Вектор- Бест». – 2018. – № 1. – С. 2–10. Bondarenko EI, Socinova LD, Timofeev DI, Mishanova EV. (2018). Detection of genetic markers of pathogens of tick-borne rickettsiosis disease in PCR using sets of reagents ”DNA Realbest Rickettsiaspecies and Realbest DNA Rickettsia sibirica/Rickettsia heilongjiangensis” [Vyyavlenie geneticheskikh markerov vozbuditeley kleshchevykh rikketsiozov v PTsR s pomoshch’yu naborov reagentov «RealBest DNK Rickettsiaspecies» i «RealBest DNK Rickettsia sibirica/Rickettsia heilongjiangensis»]. Novosti «Vektor-Best», (1), 2-10.

3. Емельянов А.Н., Кижло Л.Б. Клинико-эпидемиологические особенности иксодового клещевого боррелиоза в Забайкальском крае // Сибирский медицинский журнал. – 2012. – Т. 11, № 5. – С. 103–104. Emelyanova AN, Kizhlo LB. (2012). Clinical and epidemiological features of ixodic tick borreliosis in the Trans-Baikal territory [ Kliniko- epidemiologicheskie osobennosti iksodovogo kleshchevogo borrelioza v Zabaykal’skom kra]. Sibirskiy meditsinskiy zhurnal, (5), 103-104.

4. Карпенко С.Ф., Галимзянов Х.М. Лихорадка Ку в Астраханской области: современные реалии эпидемиологии и клиники // РЭТ-Инфо. – 2017. – № 7. – С. 5–10. Karpenko SF, Galimzyanov HM. (2017). Q-fever in the Astrakhan region: modern realities of epidemiology and clinics [Likhoradka Ku v Astrakhanskoy oblasti: sovremennye realii epidemiologii i kliniki]. RET-Info, (7), 5-10.

5. Нафеев А.А., Безик В.В. Случай лихорадки Ку на неэндемичной территории // Эпидемиология и инфекционные болезни. – 2012. – № 6. – С. 38–40. Nafeev AA, Bezik VV. (2012). Case of Q-fever in non-endemic area [Sluchay likhoradki Ku na neendemichnoy territorii]. Epidemiologiya i infektsionnye bolezni, (6), 38-40.

6. Савельева М.В., Краснова Е.И. Особенности клиники и лабораторной диагностики заболеваний, вызванных боррелиями, у жителей Новосибирской области // Журнал инфектологии. – 2018. – Т. 10, № 2. – С. 93.

7. Saveliev MV, Krasnova EI. (2018). Features of clinic and laboratory diagnostics of diseases caused by borreliae, the inhabitants of Novosibirsk region [ Osobennosti kliniki i laboratornoy diagnostiki zabolevaniy, vyzvannykh borreliyami, u zhiteley Novosibirskoy oblasti]. Zhurnal infektologii, (2), 93.

8. Сарксян Д.С., Малеев В.В., Платонов А.Е. Дифференциальная диагностика иксодового клещевого боррелиоза, вызванного Borrelia miyamotoi // Инфекционные болезни. – 2012. – Т. 10. – С. 41–44. Sarkisyan DS, Maleev VV, Platonov AE. (2012). Differential diagnosis of Ixodes tick-borne borreliosis caused by Borrelia miyamotoi [Differentsial’naya diagnostika iksodovogo kleshchevogo borrelioza, vyzvannogo Borrelia miyamotoi]. Infektsionnye bolezni, 41-44.

9. Фетисова Н.Ф., Гафарова М.Т. Эколого-эпидемиологические аспекты коксиеллёза // Вестник РАМН. – 2008. – № 7. – С. 15–18. Fetisova NF, Gafarova MT. (2008). Еcological and epidemiological aspects of coxyellosis [Ekologo-epidemiologicheskie aspekty koksielleza]. Vestnik RAMN, (7), 15-18.

10. Pieter J, Sprong H. (2015). Borrelia miyamotoi: a widespread tick-borne relapsing fever spirochete. Trends in Parasitology, 6, 260-268.

11. Platonov A, Karan L. (2011). Humans infected with relapsing fever spirochete Borrelia miyamotoi. Emerging Infectious Diseases, 17, 1816-1823.

12. Fukunaga М, Takahash Y. (1995). Genetic and phenotypic analysis of Borrelia miyamotoi sp. nov., isolated from the ixodid tick Ixodes persulcatus, the vector for Lyme disease in Japan. Int J Syst Bacteriol, 4, 804-810.