Application of membrane-protectant in the process of increasing the viability of autologous fat graft
https://doi.org/10.29413/ABS.2025-10.5.24
Abstract
Background. Autologous adipose tissue transplantation is becoming increasingly popular in reconstructive surgery, but the main unsolved problem at the moment is the high percentage of partial volume loss due to autograft resorption.
The aim. Evaluation of the viability of adipocytes under incubation in solutions of different biochemical compositions, and clinical testing of the effectiveness of an optimized fat graft.
Materials and methods. The comparative spectral analysis of the content of ions (mainly oxygen) in the cytoplasm of fat cells grown from solution samples on a solid substrate using a scanning electron microscope in low vacuum was performed. The composition in 3 samples that spent 6 h in artificial solutions was investigated. The EDAX TEAM program was used to analyze the energy dispersive X-ray spectroscopy data. Results. Statistical and morphological analysis of the obtained results revealed differences in the composition of viable cells in the studied samples, varying up to 50 %. The most effective was the solution with dimethyloxobutylphosphonyldimethylate, which demonstrated an optimal level of oxygen ion content (O), as well as pronounced integrity of the cell membrane compared to other samples during electron microscopy and histological examination.
Conclusion. One of the key factors is the medication support of the autograft during the initial stages of engraftment after transplantation. By measuring the ionic content of the intracellular matrix, we were able to examine in vitro the effect of solutions of different substances to achieve this goal. For autograft preservation, the best option is a solution with a membrane protector dimethyloxobutylphosphonyldimethylate for its ability to preserve cell homeostasis.
About the Authors
V. Ya. Kolesnik
Russian Biotechnological University
Russian Federation
Russian Federation
Vasily Ya. Kolesnik – graduate student,
Volokolamskoe highway, 11, 125080 Moscow
R. A. Pakhomova
Russian Biotechnological University
Russian Federation
Russian Federation
Regina A. Pakhomova – Dr. Sc. (Med.), Head of the Department of Plastic Surgery,
Volokolamskoe highway, 11, 125080 Moscow
A. A. Kolesnik
Russian Biotechnological University
Russian Federation
Russian Federation
Anastasia A, Kolesnik – graduate student,
Volokolamskoe highway, 11, 125080 Moscow
References
1. Voblaya IN, Koroleva NV, Moreeva EG. Marketing analysis of the plastic surgery market in Russia. Problems of Social Hygiene, Public Health and History of Medicine. 2020; 28(2): 227-233. (In Russ.). doi: 10.32687/0869-866X-2020-28-2-227-233
2. Martyusheva AS, Soldatkina DA. Prospects of plastic surgery in 2020. Bulletin of the Council of Young Scientists and Specialists of the Chelyabinsk Region. 2021; 1(32): 30-33. (In Russ.).
3. Lavrentyeva EA, Khlystalov MV, Moroz SE, Khodorovich OS, Korolev AG, Ibadullaeva SS, et al. Histological comparison of lipofilling and a hyaluronic acid-based drug after administration to rats after 30 days. Reports of the Russian Academy of Sciences. Life Sciences. 2025; 520(1): 57–62. (In Russ.). doi: 10.31857/S2686738925010101
4. Brébant V, Weiherer M, Noisser V, Seitz S, Prantl L, et al. Implants Versus Lipograft: Analysis of Long-Term Results Following Congenital Breast Asymmetry Correction. Aesthetic Plastic Surgery. 2022; 46: 2228–2236. doi: 10.1007/s00266-022-02843-5
5. Marinicheva EV, Marinicheva IG, Manturova NE, Ganshin IB. Lipofilling or silicone implants: composite plasty of the lower limbs. Plastic Surgery and Aesthetic Medicine. 2022; 4: 10-17. (In Russ.). doi: 10.17116/plast.hirurgia202204110
6. Markov SN, Spiridonov AA, Slepov AV. Possibility of fat autotissue application for stage closure of the shin wound. Wounds and wound infections. Journal of Prof. B.M. Kostyuchyonok. 2021; 8(3): 48-53. (In Russ.). doi: 10.25199/2408-9613-2021-8-3-46-51
7. Khramtsova NI, Plaksin SA, Sotskov AY, Ponomarev DN. Application of the technique of autotransplantation of adipose tissue in patients with contour deformities, skin and soft tissue defects. Perm Medical Journal. 2022; 39(4): 97-107. (In Russ.). doi: 10.17816/pmj39497-107
8. Dashtoyan GE, Startseva OI. Investigation of the influence of mesenchymal stem cells on the engraftment of fat autotransplants: relevance, efficiency, oncologic precaution. Modern Science: Actual Problems of Theory and Practice. Series: Natural and Technical Sciences. 2022; 08: 164-173. (In Russ.). doi: 10.37882/2223-2966.2022.08.12
9. Sterodimas A. Helium Plasma Effect on Breast Stromal-Enriched Lipograft: A Case Report. Aesthetic Surgery Journal Open Forum. 2024; 6: ojae087. doi: 10.1093/asjof/ojae087
10. Peer LA. Cell survival theory versus replacement theory. Plastic and Reconstructive Surgery. 1955; 16(3): 161- 168.
11. Eto H, Kato H, Suga H, Aoi N, Doi K, Kuno S, et al. The fate of adipocytes after nonvascularized fat grafting: evidence of early death and replacement of adipocytes. Plastic and Reconstructive Surgery. 2012; 129(5): 1081-1092. doi: 10.1097/PRS.0b013e31824a2b19
12. Lee K, Jeong W, Choi J, Kim J, Son D, Jo T. Fat graft survival requires metabolic reprogramming toward the glycolytic pathway. Journal of Plastic, Reconstructive and Aesthetic Surgery. 2023; 83: 23-31. doi: 10.1016/j.bjps.2023.04.010
13. Bogdanova NM, Novikova VP, Khavkin AI, Dekhtyareva NS. Lactate and lactoacidosis: the main pathogenetic mechanisms in the development of diseases. Voprosy Dietologii. 2020; 10(1): 45- 52. (In Russ.). doi: 10.20953/2224-5448-2020-1-45-52
14. Vizel AA, Vizel AO, Shchukina LI. Dimethyl oxobutylphosphonyl dimethylate (Dimephosphon): application in pulmonology and phthisiology. Atmosfera. Pulmonologiya i allergologiya. 2013; 3: 40-44 (In Russ.).
15. Yang H, Acker J, Chen A, McGann L. In situ assessment of cell viability. Cell Transplant. 1998; 7(5): 443-51. doi: 10.1177/096368979800700503
16. Luze H, Einsiedler J, Nischwitz SP, Winter R, Kolb D, Kamolz LP, et al. Quality and Vitality of Autologous Fat Grafts Harvested by Different Techniques: A Clinical Comparison Study. Aesthet Surg J. 2022; 42(12): 1416-1424. doi: 10.1093/asj/sjac192
17. Urakov AL, Urakova AL, Shabanov PD. Hypoxic irreversible brain cells damage, associated risk factors and antihypoxants. Reviews on Clinical Pharmacology and Drug Therapy. 2024; 22(3): 277-288. doi: 10.17816/ RCF629408
18. Ignatenko GA, Bondarenko NN, Tumanova SV. Hypoxia-Inducible Factors: Details Create a “Picture”. Part I. HIF-1. Fundamentalnaia i klinicheskaia meditsina. 2023; 8(3): 93-106. (In Russ.). doi: 10.23946/2500-0764-2023-8-3-93-106
19. Artamonov AA, Ivanov AM. Scanning electron microscopy in hematology. Laboratornaia sluzhba. 2025; 14(1): 38-47. (In Russ.). doi: 10.17116/labs20251401138
20. Maksimova VD, Shakirova SM. Features of scanning microscopy. Student and agricultural science: Proceedings of the XVIII All-Russian student scientific conference of 2024. 2024: 318-321. (In Russ.).
21. Yurova EV, Rastorgueva EV, Beloborodova EA. Peptide toxin targeting sodium channels in a model of ischemia-reperfusion cell death. Ulianovskii mediko-biologicheskii zhurnal. 2025; 1: 93-103. (In Russ.). doi: 10.34014/2227-1848-2025-1-93-103
22. Kozlova MI, Bushmakin IM, Belyaeva YuD. Expansion of the “Sodium World” through evolutionary time and taxonomic space. Biochemistry. 2020; 85(12): 1788-1815. (In Russ.). doi: 10.31857/S0320972520120052
23. Plakhotnaya VM, Martynova EY, Poteshkin YuE. Sodium-glucose cotransporter 1: role in the pathogenesis of type 2 diabetes mellitus and other diseases and a potential target for therapeutic intervention. Effektivnaia farmakoterapiia. 2022; 18(2): 42-54. (In Russ.). doi: 10.33978/2307-3586-2022-18-2-42-54
Review
For citations:
Kolesnik V.Ya., Pakhomova R.A., Kolesnik A.A. Application of membrane-protectant in the process of increasing the viability of autologous fat graft. Acta Biomedica Scientifica. 2025;10(5):224-232. (In Russ.) https://doi.org/10.29413/ABS.2025-10.5.24
Views:
58
.png)
Email this article 
