Assessment of the parameters of the fatty acid spectrum of blood serum in relation to hormonal status in obese adolescents
https://doi.org/10.29413/ABS.2025-10.5.18
Abstract
Rationale. The development and implementation of new high-tech mass spectrometric diagnostic methods into laboratory practice has determined the possibility of a global analysis of the human lipidome, in particular, for a detailed study of its fatty acid component and assessment of the role of individual free fatty acids (FFA) in the pathogenesis of obesity and associated diseases.
Objective. To identify the features of changes in the parameters of the fatty acid spectrum of blood serum and establish their relationship to hormonal status indicators in adolescents with obesity.
Materials and methods. A total of 27 adolescents aged 10–18 years with obesity (SDS BMI 2.0-3.9) were examined. The control group consisted of 27 adolescents with normal weight with comparable characteristics by gender and age. SDS BMI was calculated using the WHO Anthroplus calculator. The concentration of hormones and peptides in the blood serum was measured by ELISA. The mobile fatty acid pool of blood serum was assessed by chromatography-mass spectrometry on an Agilent 7000B detector.
Results. In adolescents with obesity of 1–3 degrees, elevated levels of insulin and C-peptide, decreased concentration of GLP-2 and fatty acid imbalance (decreased proportion of GLA, DGLA, DPA, DHA, AA and increased content of ALA, OA, POA, BA, MA, PA, MAA), as well as a low risk index for the development of a subintimal inflammatory reaction are recorded in the blood. In the group with obesity, direct and negative correlations were established between the content of individual hormones and fatty acids, which were absent between the corresponding parameters in the group of healthy individuals.
Conclusion. The established endocrine-metabolic changes in adolescents with obesity are pathogenetic factors of a complex of compensatory-adaptive reactions accompanying low-intensity inflammation.
About the Authors
I. E. Esimova
Siberian State Medical University
Russian Federation
Russian Federation
Irina E. Esimova – Dr. Sc. (Med.), Professor of the Department of Biochemistry and Molecular Biology with a course in Clinical Laboratory Diagnostics,
Moskovsky trakt 2, Tomsk 634050
O. V. Voronkova
Siberian State Medical University
Russian Federation
Russian Federation
Olga V. Voronkova – Dr. Sc. (Med.), Assistant Professor, Head of the Department of Biology and Genetics,
Moskovsky trakt 2, Tomsk 634050
Iu. G. Samoilova
Siberian State Medical University;
Institute of Medicine and Medical Technologies Novosibirsk State University
Russian Federation
Russian Federation
Iuliia G. Samoilova – Dr. Sc. (Med.), Professor, Professor of the Department of Pediatrics with a course in Endocrinology, Moskovsky trakt 2, Tomsk 634050;
Director, Pirogov Street, 1, Novosibirsk Oblast, Novosibirsk 630090
D. V. Podchinenova
Siberian State Medical University;
Institute of Medicine and Medical Technologies Novosibirsk State University
Russian Federation
Russian Federation
Daria V. Podchinenova – Cand. Sc. (Med.), Associate Professor of the Department of Pediatrics with a course in Endocrinology, Moskovsky trakt 2, Tomsk 634050;
Deputy Director, Pirogov Street, 1, Novosibirsk Oblast, Novosibirsk 630090
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Review
For citations:
Esimova I.E., Voronkova O.V., Samoilova I.G., Podchinenova D.V. Assessment of the parameters of the fatty acid spectrum of blood serum in relation to hormonal status in obese adolescents. Acta Biomedica Scientifica. 2025;10(5):155-166. (In Russ.) https://doi.org/10.29413/ABS.2025-10.5.18
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