Indicators of immunocompetent blood cells of experimental animals immunized against plague on the background of immunomodulation

Abstract

The strategy of specific prophylaxis of plague for epidemic indications accepted in the Russian Federation, while having positive experience, needs to be improved. The solution to one of the problems is the use of vaccines together with adjuvants. The ability of the selenium-containing preparation 974zh to increase the immunogenicity of live plague vaccine was demonstrated.
The aim of the work. To evaluate the subpopulation composition of blood cells in animals immunized by Y. pestis EV on the background of immunomodulation.
Materials and methods. In the study we used 100 white mice. Blood served as a material for the study. The phenotype of lymphocytes was determined on a flow cytofluorimeter using antibodies to markers CD45, CD44, CD3, CD19, CD4, CD8, CD25, CD62L, I-A/I-E (MHC II).
Results. Evaluation of cellular immunity revealed a statistically significant increase in monocytes on day 7 and a decrease in lymphocytes on day 3 in mice immunized with Y. pestis EV at doses of 10³ CFU and 10⁴ CFU in combination with 974zh. When co-injected with Y. pestis EV (103 CFU) and 974zh, unlike Y. pestis EV at different doses, no decrease in CD3⁺CD4⁺CD8^-CD25⁺ cells was detected.
Conclusion. Thus, the adjuvant property of 974zh has been established, contributing to an increase in the immunogenic properties of the Y. pestis EV vaccine strain, regardless of the dose. The use of Y. pestis EV at a dose of 10³ CFU to reduce the bacterial load is appropriate.