Age peculiarities in the change of aldoketoreductase spectrum in rat blood

Abstract

In order to reveal the causes of age-related changes in sensitivity of the organism to oxidative stress, the spectrum of aldoketoreductases in blood serum of rats of different ages was investigated. It was discovered that 0.5- and 24-month old rats have the similar spectrum of aldoketoreductase in the blood. There are only four electrophoretic fractions of aldoketoreductases in their spectrum. The share of 3^rd fraction increases the spectrum of 1.5-, 2- and 3- month old rats. The pubertal rats contain additional 5^th fraction in aldoketoreductase spectrum of the blood. This electrophoretic fraction has maximal electrophoretic mobility, but there is inconstancy in spectrum. There are 6 electrophoretic fractions of aldoketoreductases in the blood of 3-month old rats. Two of them with maximal electrophoretic mobility are absent in the spectrum of 0.5- and 24- month old rats. There are the same shares of 3^rd and 4^th fraction in the aldoketoreductase spectrum of the blood 12-month old rats, and 5^th fraction there is inconstancy. The cause of age-related changes in the aldoketoreductase spectrum of blood may be coupled with existence of age-related differences in the gene expression of the encoded enzymes of aldoketoreductase family. Due to the fact that 0.5- (early childhood) and 24-month old (senescence) rats have similar aldoketoreductase spectrum of the blood, we propose that the cause ofthat phenomenon coupled with age-related changes in the production and secretion of sex steroids.