Morphometric analysis of the growth factors influence on fibroblastic phase of inflammation at experimental myocardial infraction

Abstract

The objective of the study was comparative study of the effect of VEGF and FGF on fibroblastic phase of inflammation by replacing necrotic myocardium on its model of acute myocardial infarction. Model of myocardial infarction in rats was carried out to study the effect of increasing and decreasing concentrations of basic fibroblast growth factor (FGF2) and vascular endothelial growth factor (VEGF) on the dynamics of morpho-metric parameters fibroblastic phase. It has been established that the increase of VEGF in the systemic circulation more stimulated the growth of fibroblasts compared to FGF. Thus, the density of fibroblasts in the repair area in VEGF group during all periods of observation values in a group exceeds FGF. Differences were significant at the 7^th (295 [249-340] versus 205 [192-275], p = 0.0257) and the 30^th day (132 [125-148] versus 88 [81-122], p = 0.0036). However, when reducing the amount of growth factors by introducing antibodies fibroblastic cells showed greater sensitivity to the reduction of FGF. Fibroblast density in the area of repair in the group at all time points antiVEGF observation group was significantly higher performance antiFGF: the 3^rd day 299 [130-481] and 132,5 [92-148], p = 0,0257; the 7^th day - 306 [272-369] and 167 [151-215], p = 0,0008; at the 14^th day - 250.5 [233-260] and 131,5 [121-147], p = 0,0002; on the 30^th day - 195.5 [139-237] and 88,5 [81-96], p = 0.0003 respectively. It was established, that modifying the natural regulatory stimulation of both the side with the additional administration the growth factors and decreasing its level at the expense of elimination upon binding to antibody leads to disruption of fibroblast differentiation to fibrocytes.