Proteolytic cleavage of immunoglobulins G by subcellular fractions of Vibrio Cholerae O1 and O139 serougroups
Abstract
With the use of diffusion test in substrate agarose gel in sterile subcellular fractions of Vibrio cholerae 01 and 0139 serogroups, got from by the lysis urea of living microbial cells, found out a ability for the proteolysis of rabbit and human immunoglobulins of class G. Substrate electrophoresis detected polypeptides with IgG-protease activity both in water-soluble and membrane fractions of the strains with different molecular masses. Differences in IgG-protease activity of subcellular fractions of V. cholerae ompT+ and ompT-strains were determined.
About the Authors
S. N. Kozlov
Irkutsk Antiplague Research Institute of Rospotrebnadzor
Russian Federation
Russian Federation
V. B. Nikolaev
Irkutsk Antiplague Research Institute of Rospotrebnadzor
Russian Federation
Russian Federation
E. Y. Markov
Irkutsk Antiplague Research Institute of Rospotrebnadzor
Russian Federation
Russian Federation
L. V. Mironova
Irkutsk Antiplague Research Institute of Rospotrebnadzor
Russian Federation
Russian Federation
L. Y. Urbanovich
Irkutsk Antiplague Research Institute of Rospotrebnadzor
Russian Federation
Russian Federation
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Review
For citations:
Kozlov S.N., Nikolaev V.B., Markov E.Y., Mironova L.V., Urbanovich L.Y. Proteolytic cleavage of immunoglobulins G by subcellular fractions of Vibrio Cholerae O1 and O139 serougroups. Acta Biomedica Scientifica. 2013;(6):145-148. (In Russ.)
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