INVESTIGATION PHYLOGENETIC RELATIONSHIPS MAJOR GENOTYPES MYCOBACTERIUM TUBERCULOSIS BY MIRU-VNTR-24 GENOTYPING
Abstract
Thirty-one isolates of M. tuberculosis was identified in Buryatia by MIRU-VNTR-24 typing and deletion analysis by RD105 and RD207. Beijing family strains accountedfor 67,4 % and had a variety of profiles with a prevalence (22,6 %) of two modern epidemic subtype Beijing MIT17 and MIT16. Strains Beijing MIT 642 differed in the number of repeats of the variable loci, it may reflect a longer circulation of these subtypes without shaping their properties of epidemic circulating strains in modern populations, or they had epidemic importance in the past. It is also apparent heterogeneity in the number of loci among strains according to group LAM, T, and X, which requires additional research. The aim of this work was to determine the current epidemiologically important genotypes of Mycobacterium tuberculosis, as well as the genotypes had an epidemic importance in the past as an example of the region with a high prevalence of tuberculosis - the Republic of Buryatia.
About the Authors
A. A. Lats
Scientific Centre of the Family Health and Human Reproduction Problems Siberian Branch, Russian Academy of Medical Sciences
Russian Federation
Russian Federation
O. B. Ogarkov
Scientific Centre of the Family Health and Human Reproduction Problems Siberian Branch, Russian Academy of Medical Sciences
Russian Federation
Russian Federation
S. N. Zhdanova
Scientific Centre of the Family Health and Human Reproduction Problems Siberian Branch, Russian Academy of Medical Sciences
Russian Federation
Russian Federation
A. N. Zarbuev
Republican Clinical TB Dispensary
Russian Federation
Russian Federation
L. S. Untanova
Republican Clinical TB Dispensary
Russian Federation
Russian Federation
M. V. Badleeva
Buryatian State University
Russian Federation
Russian Federation
B. D. Zanaeva
Scientific Centre of the Family Health and Human Reproduction Problems Siberian Branch, Russian Academy of Medical Sciences
Russian Federation
Russian Federation
O. Ts. Tsydypova
Scientific Centre of the Family Health and Human Reproduction Problems Siberian Branch, Russian Academy of Medical Sciences
Russian Federation
Russian Federation
References
1. Молекулярное типирование штаммов микобактерий туберкулеза в Иркутской области (Восточная Сибирь) в 2000-2005 гг. / О.Б. Огарков [и др.] // Молекулярная медицина. - 2007. - № 2. - С. 33-38.
2. Полиморфизм Mycobacterium tuberculosis, выделенных от больных туберкулезом в пенитенциарной системе Бурятии: высокая распространенность генотипа пекин / О.Б. Огарков [и др.] // Сибирский медицинский журнал (Иркутск). - 2012. - № 6. - С. 54-57.
3. Характеристика лекарственно устойчивых штаммов Mycobacterium tuberculosis с помощью молекулярно-генетических методов / С.Н. Жданова [и др.] // Сибирский медицинский журнал (Иркутск). - 2011. - № 6. - С. 228-230.
4. Evaluation of a two-step approach for large-scale, prospective genotyping of Mycobacterium tuberculosis isolates in the United States / L. Cowan [et al.] // J. Clin. Microbiol. - 2005. - № 43. - P. 688-695.
5. Extremely high prevalence of multidrug resistant tuberculosis in Murmansk, Russia: a population-based study / J. Ma'kinen [et al.] // Eur. J. Clin. Microbiol. Infect. Dis. - 2011. - № 30 -Р. 1119-1126.
6. Genetic analysis of mycobacterium tuberculosis strains isolated in Ural region, Russian Federation, by MIRU-VNTR genotyping / S.Y. Kovalev [et al.] // Int. J. Tuberc. Lung Dis. - 2005. - № 9. - P. 746-752.
7. Genetic Diversity of the Mycobacterium tuberculosis Beijing Family Based on SNP and VNTR Typing Profiles in Asian Countries / Y.-Y. Chen [et al.] // PLoS ONE 7(7):e39792. doi:10.1371/journal. pone.0039792.
8. Hypervariable loci that enhance the discriminatory ability of newly proposed 15-loci and 24-locivariable-number tandem repeat typing method on Mycobacterium tuberculosis strains predominated by the Beijing family / T. Iwamoto [et al.] // FEMS Microbiol Lett. - 2007. -№ 270. - P. 67-74.
9. Large Sequence Polymorphisms Classify Mycobacterium tuberculosis Strains with Ancestral Spoligotyping Patterns / L. Flores [et al.] // J. Of Clin. Microbiol. - 2007. - № 45. - P. 3393-3395.
10. Major Mycobacterium tuberculosis Lineages Associate with Patient Country of Origin / M. Reed [et al.] // J. Clin. Microbiol. - 2009. - № 47. - P. 1119-1128.
11. Markelov Y., Narvskaya O. Circulation of multidrug-resistant tuberculosis pathogen strains in the Republic of Karelia // Tuberk. Bolezn. Legk. - 2010. -№ 2. - Р. 54-56.
12. Mycobacterium tuberculosis population in northwestern Russia: an update from Russian-EU-Latvian border region / I. Mokrousov [et al.] // PLoS One. 2012;7(7):e41318. doi: 10.1371/journal. pone.0041318.
13. Narvskaya O., Mokrousov I., Otten T., Vishnevsky B. Molecular markers: application for studies of Mycobacterium tuberculosis population in Russia / Read M.M., editor. Trends in DNA fingerprinting research // Nova Science Publishers. - 2005. - №7. - P. 111-125.
14. Proposal for standardization of optimized mycobacterial interspersed repetitive unit-variablenumber tandem repeat typing of Mycobacterium tuberculosis / P. Supply [et al.] // J. Clin. Microbiol. - 2006. - № 44. - Р. 498-510.
15. Spread of drug-resistant Mycobacterium tuberculosis strains of the Beijing genotype in the Archangel Oblast, Russia / O.S. Toungoussova [et al.] // J. Clin. Microbiol. - 2002. - № 40. - Р. 1930-1937.
16. The genetic diversity of Mycobacterium tuberculosis and an assessment of risk factors of tuberculosis spread in Russia's Siberian region by molecular epidemiological methods / O.V. Norkina [et al.] // Mol. Gen. Mikrobiol. Virusol. - 2003. - № 3. - Р. 9-18.
17. Velji P., Nikolayevskyy V., Brown T., Drobniewski F. Discriminatory ability of hypervariable variable number tandem repeat loci in population-basedanalysis of Mycobacterium tuberculosis strains, London, UK // Emerg. Infect. Dis. - 2009. - № 15. - Р. 1609-1616.
18. World Health Organization, Annual Meeting of the European Network for TB Surveillance in Europe, Dubrovnik, Croatia 25-26 May: Tech. rep.: WHO. - 2009.
Review
For citations:
Lats A.A., Ogarkov O.B., Zhdanova S.N., Zarbuev A.N., Untanova L.S., Badleeva M.V., Zanaeva B.D., Tsydypova O.Ts. INVESTIGATION PHYLOGENETIC RELATIONSHIPS MAJOR GENOTYPES MYCOBACTERIUM TUBERCULOSIS BY MIRU-VNTR-24 GENOTYPING. Acta Biomedica Scientifica. 2013;(2(2)):144-147. (In Russ.)
Views:
412
.png)
Email this article 
