CARBONIC ANHYDRASE AS A MARKER OF OSTEOCLAST ACTIVITY IN THE REPAIR ZONE OF LONG BONE FRACTURE
Abstract
In an experimental model of long bone fractures studied osteoclastic activity in the fracture zone. We used histological staining methods and histochemical staining with antibodies to carbonic anhydrase type II. It is shown that the use of histochemical staining to carbonic anhydrase type II cells is promising for the evaluation of osteoclastic activity in bone tissue. Application of this technique significantly simplifies the morphological study, allows a clear differentiation of osteoclastic cells.
About the Authors
I. A. Shurygina
Scientific Center of Reconstructive and Restorative Surgery SB RAMS
Russian Federation
Russian Federation
M. G. Shurygin
Scientific Center of Reconstructive and Restorative Surgery SB RAMS
Russian Federation
Russian Federation
L. V. Rodionova
Scientific Center of Reconstructive and Restorative Surgery SB RAMS
Russian Federation
Russian Federation
V. Ph. Lebedev
Scientific Center of Reconstructive and Restorative Surgery SB RAMS
Russian Federation
Russian Federation
N. N. Dremina
Scientific Center of Reconstructive and Restorative Surgery SB RAMS
Russian Federation
Russian Federation
References
1. Krishnamurthy V.M., Kaufman G.K., Urbach A.R. et al. Carbonic anhydrase as a model for biophysical and physical-organic studies of proteins and protein-ligand binding // Chem. Rev. - 2008. -Vol. 108. - P. 946-1051.
2. Mori K., Ogawa Y., Ebihara K. et al. Isolation and characterization of CA XIV, a novel membrane-bound carbonic anhydrase from mouse kidney // J. Biol. Chem. - 1999. - Vol. 274, N 22. - P. 15701 -15705.
3. Oksala N., Levula M., Pelto-Huikko M. et al. Carbonic anhydrases II and XII are up-regulated in osteoclast-like cells in advanced human atherosclerotic plaques-Tampere Vascular Study // Ann. Med. — 2010. — Vol. 42, N 5. — P. 360 — 370.
4. Shah G.N., Hewett-Emmett D., Grubb J.H. et al. Mitochondrial carbonic anhydrase CA VB: differences in tissue distribution and pattern of evolution from those of CA VA suggest distinct physiological roles // Proc. Natl. Acad. Sci. USA. — 2000. — Vol. 97, N 4. — P. 1677 — 1682.
Review
For citations:
Shurygina I.A., Shurygin M.G., Rodionova L.V., Lebedev V.P., Dremina N.N. CARBONIC ANHYDRASE AS A MARKER OF OSTEOCLAST ACTIVITY IN THE REPAIR ZONE OF LONG BONE FRACTURE. Acta Biomedica Scientifica. 2012;(5(2)):120-122. (In Russ.)
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