NECESSITY OF AGE-RELATED STANDARDS FOR IMMUNE PHENOTYPING OF BLOOD CELLS FOR MONITORING OF HEALTH IN CHILDREN BORN BY HIV-INFECTED WOMEN

Considering the increase in number of HIV-infected women of childbearing age this study was aimed at a comparison of CD-phenotype of children born by infected mothers with age standards obtained during the analysis of the functional state of the immune system of healthy children differing by sex and age in the Perm region. 133 children from infected mothers at the age from 1 month to 4 years and 53 clinically healthy children were examined. Among the peripheral blood lymphocytes of children the numbers of CD3+-T-lymphocytes, CD3+CD4+-T-helpers, CD3+CD8+-cytotoxic T-lymphocytes, CD3-CD19+-B-lymphocytes, CD3-CD16+/56+-NK-cells were identified. The increase in the functional activity of the immune system of the risk group was reliably determined. In HIV-positive children of 1 to 6 months and 1 to 4 years the increase in the absolute number of leukocytes, lymphocytes, T-lymphocytes, cytotoxic T-lymphocytes, B-lymphocytes was oserved as compared with their healthy coevals. By contrast, under similar comparison within the age group of 6 to 12 months the decrease in percentage of lymphocytes and absolute number of NK-cells was determined. Gender-based division of children under study supported the necessity in taking into account this factor yet in this early age. It was shown that both in perinatal contact and under the progression of HIV infection the reaction of male and female bodies differed. Data obtained from the phenotyping of blood from healthy children in the city of Perm were compared with those provided from American and Chinese investigations. Resulting differences primarily depended on the age range of the children and were frequently related to the absolute cell number that expressed definite markers. Results evidence for the necessity in further temporal specification under the assessment of the state of the immune system in children, and allow developing new criteria for immune system monitoring under the risk of vertical route of HIV infection.

HIV, children, age, immunophenotyping

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