Ratio of peripheral blood leukocytes in young women of the arctic zone of the Russian Federation

Seven subjects of the Arkhangelsk Region, and also the Northern Administrative District and the Yamalo-Nenets Autonomous District are part of the AZRF, where climatic and ecological conditions are characterized as extreme (the industry of the Central Industrial Complex, the military industrial complex, the cosmodrome, and the Neftegazprom). Part of the territory of the Arkhangelsk region and the NAO are adjacent to the nuclear test zone (NPS) at the Northern Test site from 1955 to 1990. Nuclear weapons have never been produced in Western Siberia (Yamalo-Nenets Autonomous District). The relevance of the study is determined by the lack of information about the immune status of young northern women who were born and permanently reside in the Arctic zone of the Russian Federation.

The aim. To identify the ratio of peripheral blood leukocyte levels in young women from different northern regions.

Materials and methods. Sixty two women were examined: 47 people from the Nenets Autonomous Okrug and the Arkhangelsk Region (22–35 years old) and 15 people aged 22–40 years (Yamalo-Nenets Autonomous Okrug). Leukocytes, NLR, CD8+, and CD16+ cells were determined. An indirect immunoperoxidase reaction with monoclonal antibodies was used. Microsoft Excel 2010 and SPSS 20.0 for Windows were used.

Results. Residents of the adjacent area had elevated concentrations of monocytes (20.0; 12.5; 43.7 %), eosinophils (20.0; 25.0; 12.5 %), CD8+ lymphocytes (73.3; 31.2; 75.0%), and CD16+ lymphocytes (33.3; 62.5; 43.7 %). Deficiency of the total number of lymphocytes was 2.5 times more common than lymphocytosis: 20.0, 43.7, 12.5 %. Increased concentrations of monocytes 46.7 %, eosinophils 26.7 %, neutrophils 20.0 %, leukocytes %, CD8+ (80.0 %) and CD16+ (66.7 %) lymphocytes were recorded in residents of the non-adjacent zone.

Conclusion. The ratio of lymphocyte subpopulations in young women living in the Arctic zone of the Russian Federation is characterized by a high concentration of monocytes, eosinophils, and lymphocytes (CD8+, CD16+) against the background of a pronounced deficiency of the total lymphocyte population.

1. Arctic Zone of the Russian Federation (AZRF)]. URL: https://neftegaz.ru/tech-library/transportirovka-i-khranenie/668885-arkticheskaya-zona-rossiyskoy-federatsii-azrf-/ [date of access: September 29, 2025].

2. Staroded AS, Maidan VA, Tsvetkov SV. Influence of medico-geographical features of the Far North on the processes of adaptation. Proceedings of the Russian Military Medical Academy. 2020; 39(3-5): 160-163. (In Russ.).

3. Pashinskaya KO, Samodova AV, Dobrodeeva LK. Features of the immune system and levels of blood transport components in residents of the arctic of the Russian Federation. Am J Hum Biol. 2024; 36(10):e24136. doi: 10.1002/ajhb.24136

4. Lushnov AM, Lushnov MS. Medical information systems: multivariate analysis of medical and environmental data. St. Petersburg. “Helicon Plus. 2013; 460. (In Russ.).

5. Gmoshinsky IV, Nikityuk DB. Polar stress: mechanisms and modeling in the experiment. Bulletin of the Russian Academy of Medical Sciences. 2022; 77(6): 447-457. (In Russ.). doi: 10.15690/vramn2209

6. Ivanov AB, Krasilov GA, Logachev VA, Matushchenko AM, Safronov VG. Northern Novaya Zemlya test site, radiological consequences of nuclear tests. Moscow. 1997; 83. (In Russ.).

7. [Korchin VI, Korchina TYa, Ternikova EM, Bikbulatova LN, Lapenko VV. Infl e of climatic and geographical factors of the Yamalo-Nenets Autonomous District on public health (review). J. Med.-biol. Res. 2021; 9(1); 77–88. (In Russ.). doi: 10.37482/2687-1491-Z046

8. Donaldson S, Adlard B, Odland JØ. Overview of Human Health in the Arctic: Conclusions and Recommendations. Arctic Monitoring and Assessment Program. International Journal of Circumpolar Health. 2016; 75: 33807. doI: 10.3402/ijch.v75.33807

9. Kurzanov AN, Zabolotskikh NV, Kovalev DV. Functional reserves of the organism. – Moscow: Publishing House of the Academy of Natural Science. 2016; 96. (In Russ.).

10. Verster JC, Kraneveld AD, Garssen J. The Assessment of Immune Fitness. Journal of Clinical Medicine. 2023; 12(1): 22. doi: 10.3390/jcm12010022

11. Krivoshchekov SG. Labor and human health in the Arctic. Vestn. Sev. (Arctic) feder. Uni. Ser.: Med.-biol. sciences. 2016; 4; 84-93. (In Russ.).

12. Malikova EL. Mapping of Aeolian relief forms of the Nadym Ob region. Geographical studies of Siberia and adjacent territories. Mat. International geographical conf. Irkutsk: Publishing House of the V.B. Sochava Institute of Geography SB RAS. 2019; 510-513. (In Russ.).

13. Gorbachev AL. Biogeochemical characteristics of the northern regions of Russia. Man in the North: systemic mechanisms of adaptation: collected works dedicated to the 90th anniversary of the founding of Magadan / ed. Academician NN Besetnova. Magadan: Express polygraphy. 2019; 3: 68–79. (In Russ.).

14. Dudarev AA, Dorofeyev VM, Dushkina EV, Alloyarov PR, et al. Food and water security issues in Russia III: Foodand Waterborne Diseases in the Russian Arctic, Siberia and the Far Еast, 2000–2011. Int. J. Circumpolar Health. 2013; 72(1): 21856. doi: 10.3402/ijch.v72i0.21856

15. Shchegoleva LS, Shashkova EYu, Sergeeva TB. Cellular immunity in women aged 20–40 living in different climatic and geographic regions. Acta biomedica scientifica. 2024; 9(2): 120-129. (In Russ.). doi: 10.29413/ABS.2024-9.2.12

16. Sabirov JB, Namazbaeva ZI, Mukasheva MA. Assessment of peripheral blood parameters in the population living near the Semipalatinsk polygon. Med. labor and industrial ecol. 2019; 59(9). (In Russ.). doi:10.31089/1026-9428-2019-599-738-739

17. Snigireva GP, Novitskaya NN. Possibilities of using cytogenetic methods in the examination of the population exposed to radiation as a result of nuclear explosions at the Semipalatinsk test site. Bulletin of the Russian Scientific Research Center for Reconstruction and Research of the Ministry of Health of the Russian Federation. 2011; 11. URL: http://vestnik.rncrr.ru/vestnik/v11/papers/snigir_v11.htm [date of access: February 02, 2025].

18. Tanaka K, Iida S, Takeichi N, Chaizhunusovaet NJ, Gusev BI, Apsalikov KN, et al. Unstable-type chromosome aberrations in lymphocytes from individuals living near Semipalatinsk nuclear test site. Journal of Radiation Research (Tokyo). 2006; 47(A): 159-164. doi: 10.1269/jrr.47.a159

19. Buonacera A, Stancanelli B, Colaci M, Malatino L. Neutrophil to Lymphocyte Ratio: An Emerging Marker of the Relationships between the Immune System and Diseases. Int J Mol Sci. 2022; 23(7): 3636. doi: 10.3390/ijms23073636

20. Zahorec R. Neutrophil-to-lymphocyte ratio, past, present and future perspectives. Bratisl Lek Listy. 2021; 122(7): 474-488. doi: 10.4149/BLL_2021_078

21. Song M, Graubard BI, Rabkin CS, Engels EA. Neutrophil-to-lymphocyte ratio and mortality in the United States general population. Sci Rep. 2021; 11(1): 464. doi: 10.1038/s41598-020-79431-7

22. Dobrodeeva LK, Samodova AV. Adhesion and aggregation of peripheral venous blood cells in inhabitants of high latitudes. Ekaterinburg: Uro RAS; 2024; 216. (In Russ.).

23. Unguryanu T, Novikov S, Buzinov R, Gudkov A, Grjibovski A. Respiratory Diseases in a Town with Heavy Pulp and Paper Industry. Epidemiologia e Prevenzione. 2010; 34(5-6): 138