Influence of a multicomponent plant substance on physical endurance in the forced swimming test

The effect of preventive administration of the complex phytopreparation "tetraphyton" has been studied in experiments on rats. Tetraphyton developed on the base of Tibetan formula is the dry extract derived from species of plant material: roots of Inula helenium L., roots of Zingiber officinale Roscoe, fruits of Elletaria cardamomum (L.) Maton, offshoots of Caragana spinosa (L.) Wall. ex Hornem., characterized for the content of phenolic compounds, flavonoids, terpenoids. The preventive course introduction of tetraphyton in a dose 100 mg/kg for 7 days before experiment increases the potential for physical performance, increasing overall physical endurance against a background of intense physical exertion by 73 % (p < 0.05), prevents fatigue. It is shown that increase in the physical performance of rats in the experimental group is due to increase in the efficiency of the energy supply system in skeletal muscles and myocardium: a significant increase in the content of ATP (by 13 % and 2.5 times); decrease in lactate and pyruvate content (by 33 and 30 %; p < 0.05); increased glycogen content in the liver by 23 %, blood glucose by 19 %. The increase in catalase, SOD, and gSh levels (5 %, 19 %, 79 %; p < 0.05) indicates an increase in the power of the antioxidant defense of the organism and causes the cytoprotective effect of tetraphyton, confirmed by a decrease in fermentemia (lactate dehydrogenase, kreatinphosphokinase - 49 % and 43 %; p < 0.05). The main molecular-cellular mechanisms of the actoprotective action of the phytoadaptogen is the inhibition of the lipid peroxidation of cell membranes and a significant increase in the capacity of the endogenous antioxidant enzyme system that promotes the preservation of the integrity of skeletal muscles and the myocardium. This is due to the presence of phenolic compounds in the chemical composition that have direct antiradical action and increase the power endogenous antioxidant system.

phytopreparation, actoprotective activity, tetraphyton, rats, antioxidants

1. Азам Н., Горошко О.А., Пахомова В.П. Антиоксидантная активность лекарственных субстанций и биологически активных веществ // Традиционная медицина. - 2009. - № 1. - С. 35-38

2. Алейникова Т.А. Рубцова Г.В. Количественное определение макроэргических соединений мышц (АТФ, креатинфосфата) // Руководство к практическим занятиям по биологической химии. - М., 1988. -С. 115-117

3. Каркищенко В.Н., Капанадзе Г.Д., Деньгина С.Е., Станкова Н.В. Разработка методики оценки физической выносливости мелких лабораторных животных для изучения адаптогенной активности некоторых лекарственных препаратов / / Биомедицина. - 2011. -№ 1. - С. 72-74

4. Колб В.Г., Камышников В.С. Клиническая биохимия. - Минск, 1976. - 231 с

5. Королюк М.А., Иванова Л.И. Методы определения активности каталазы // Лабораторное дело. 1988. - № 1. - С. 16-19

6. Меерсон Ф.З., Пшенникова М.Г Адаптация к стрессорным ситуациям и физическим нагрузкам. -М.: Медицина, 1988. - 256 с

7. Николаев С.М. Фитофармакотерапия и фитофармакопрофилактика. - Улан-Удэ: Изд-во Бурят. гос. ун-та, 2012. - 286 с

8. Темирбулатов Р.А., Селезнев Е.И. Метод повышения интенсивности свободнорадикального окисления липидсодержащих компонентов крови и его диагностическое значение // Лабораторное дело. - 1981. - № 4. - С. 209-211

9. Шантанова Л.Н., Алексеева Э.А., Осадчук Л.В. Фармакокоррекция физической работоспособности растительным средством «КАРДЕКАИМ» // Бюл. ВСНЦ СО РАМН. -2013. - № 2-2 (90). - С. 167-170

10. Anderson RL, Linas SL, Berns AS. (1977). Nonoliguric acute renal failure. N Engl J Med, 236, 1134

11. Chen H, Ju Y, Li J, Yu M. (2012). Antioxidant activities of polysaccharides from Lentinus edodes and their significance for disease prevention. Int J Biol Macromol, 50 (1), 214-218.

12. Nijveldt RJ, van Nood E, van Hoorn DE, Boelens PG, van Norren K, van Leeuwen PA. (2001). Flavonoids: a review of probable mechanisms of action and potential applications. Am J Clin Nutr, 74 (4), 418-425.

13. Tohma H, Gulçin I, Bursal E, Gören A, Alwasel SH, Köksal E. (2017). Antioxidant activity and phenolic compounds of ginger (Zingiber officinale Rosc.) determined by HPLC-MS/MS. J Food Measurement Characteriz, 11 (2), 556-566. doi: 10.1007/s11694-016-9423-z