Dynamics of ATPase activity and lipid peroxidation indicators in liver tissue in experimental peritonitis using the drug “Seroguard”

Background. Oxidative stress plays an important role in the development of peritonitis, which leads to a decrease in antioxidant level and an increase in oxidant production. Studying ATPase activity and lipid peroxidation when using new methods of treating experimental peritonitis is of interest for clinical practice.

The aim. To study the dynamics of ATPase activity and lipid peroxidation indicators in liver tissue in experimental peritonitis when using Serogard.

Methods. The study was conducted on male Wistar rats aged 6 months. All animals were subjected to experimental peritonitis using the method we developed. Animals in the control group (n = 20) were intraperitoneally injected with 3 ml of physiological solution one day after modeling peritonitis; animals in the experimental group (n = 19) were injected Serogard® (AO Pharmasintez, Russia). We determined the initial values of the indicators in healthy rats (n = 7). In animal liver tissue, total and mitochondrial ATPase activity was  studied, as  well  as the  content of  malondialdehyde (MDA) and diene conjugates (DC).

Results. It was found that the total and mitochondrial activity of ATPases was suppressed by the day 3 of experimental peritonitis. During 14 days of the experiment, ATPase activity did not reach normal levels in any of the groups. However, animals in  the  experimental group had dynamics of  a  more steady, significant increase in the total ATPase and the mitochondrial ATPase (ATP synthase) activity with a significantly higher level than in the control group. MDA and DC in the experimental group increased by days 7–14, which indicated the activity of free radical processes and was also an indicator of the dynamics of restoration of metabolic and energy processes.

Conclusion. Using Serogard in experimental peritonitis contributed to a more effective restoration of total ATPase activity and mitochondrial ATPase (ATP synthase) and on days 7–14 of the study led to an increase in the level of free radical processes activity that accompany and indicate the process of aerobic energy restoration.

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