EXPRESSION OF COLLAGENS IN THE DAMAGE AREA AT ABDOMINAL ADHESIONS
https://doi.org/10.12737/article_5a0a90604689c9.94438754
Abstract
Background. Postoperative adhesions are a serious problem in surgery. However, at the present time molecular mechanisms of the adhesion process are insufficiently studied. Aim. To study the dynamics of expression of genes encoding the synthesis of collagen in case of damage to the serosa on the example of the peritoneum in conditions of aseptic inflammation. Materials and methods. Aseptic inflammatory process in the abdominal cavity was modeled (Wistar rats, n = 40). A micro- and macroscopic picture of the damage area was studied. Immunofluorescent staining for Type I collagen (Col 1A1) was performed. The expression of genes encoding collagen of different types was evaluated using the RT2-Profiler PCR kit Array Rat Wound Healing. Results. It has been established that the adhesion process with peritoneal damage in aseptic conditions reaches its maximum by the 30th day of observation. The same period coincides with the maximum of collagen synthesis in fibroblasts in the repair area, revealed by immunofluorescence study. The interrelation of synthesis of type I and III collagens went as expected - the onset of expression of type III collagen (from day 3) is ahead of the expression of collagen type I (from day 7). Peak gene expression of collagens type I, Alpha-1 and -2; type III Alpha-1, type IV Alpha-1 and -3, type V Alpha-1, -2 and -3; type XIV Alpha-1 (Col14a1) falls on the 14th day. For the first time, active involvement of type V alpha-3 collagen in the adhesion process was noted - we detected both early (from day 1) and maximum intensive (up to 166.96 times increase in comparison with intact animals). Conclusion. Perhaps, the hyperexpression of collagen V alpha-3 that we revealed is an important link in the pathogenesis of adhesion in the abdominal cavity.
About the Authors
I. A. Shurygina
ФГБНУ «Иркутский научный центр хирургии и травматологии»
Russian Federation
Russian Federation
M. G. Shurygin
ФГБНУ «Иркутский научный центр хирургии и травматологии»; АО «Фармасинтез»
Russian Federation
Russian Federation
L. V. Rodionova
ФГБНУ «Иркутский научный центр хирургии и травматологии»
Russian Federation
Russian Federation
Е. E. Chepurnykh
ФГБНУ «Иркутский научный центр хирургии и травматологии»; ФГБОУ ВО «Иркутский государственный медицинский университет» Минздрава России
Russian Federation
Russian Federation
N. I. Ayushinova
ФГБНУ «Иркутский научный центр хирургии и травматологии»
Russian Federation
Russian Federation
References
1. Аюшинова Н., Шурыгина И., Чепурных Е., Шурыгин М., Григорьев Е. Спаечная болезнь брюшной полости - междисциплинарная проблема // Врач. -2017. - № 5. - С. 8-10. Ayushinova N, Shurygina I, Chepurnykh E, Shury-gin M, Grigoriev E. (2017). Adhesive disease of abdominal cavity - interdisciplinary problem [Spaechnaya bolezn’ bryushnoy polosti - mezhdistsiplinarnaya problema]. Vrach, (5), 8-10.
2. Аюшинова Н.И., Шурыгина И.А., Шурыгин М.Г., Лепехова С.А., Балыкина А.В., Малгатаева Е.Р., Попова А.Д., Янкелевич С.А. Экспериментальная модель для разработки способов профилактики спаечного процесса в брюшной полости // Сибирский медицинский журнал (Иркутск). - 2012. - Т. 109, № 2. - С. 51-53. Ayushinova NI, Shurygina IA, Shurygin MG, Lepekhova SA, Balykina AV, Malgatayeva ER, Popova AD, Yankelevich SA. (2012). Experimental model for the development of prevention of adhesions in the abdominal cavity [Eksperimental’naya model’ dlya razrabotki sposobov profilaktiki spaechnogo protsessa v bryushnoy polosti]. Sibirskiy meditsinskiy zhurnal (Irkutsk), 109 (2), 51-53.
3. Способ моделирования спаечного процесса в брюшной полости: Патент № 2467401 Рос. Федерация; МПК G09B 23/28 (2006.01) / Аюшинова Н.И., Лепехова С.А., Шурыгина И.А., Рой Т.А., Шурыгин М.Г., Зарицкая Л.В., Гольдберг О.А.; заявитель и патентообладатель Учреждение Российской академии медицинских наук Научный центр реконструктивной и восстановительной хирургии Сибирского отделения РАМН (НЦРВХ СО РАМН). - № 2011131678/14; заявл. 27.07.2011; опубл. 20.11.2012. - Бюл. № 32. Ayushinova NI, Lepekhova SA, Shurygina IA, Roy TA, Shurygin MG, Zaritskaya LV, Goldberg OA. (2012). Method for modeling the adhesion process in the abdominal cavity: Patent N 2467401 of the Russian Federation [Sposob modelirovaniya spaechnogo protsessa v bryushnoy polosti: Patent № 2467401 Ros. Federatsiya].
4. Шурыгина И.А., Аюшинова Н.И., Шурыгин М.Г. Оценка эффективности и безопасности применения Adept для профилактики спайкообразования в брюшной полости в эксперименте // Новости хирургии. -2017. - Т. 25, № 1. - С. 14-19. Shurygina IA, Ayushinova NI, Shurygin MG. (2017). Evaluation of the effectiveness and safety of Adept application for the prevention of adhesion in the abdominal cavity in the experiment [Otsenka effektivnosti i bezopasnosti primeneniya Adept dlya profilaktiki spaykoobrazovaniya v bryushnoy polosti v eksperimente]. Novosti khirurgii, 25 (1), 14-19.
5. Шурыгина И.А., Шурыгин М.Г., Аюшинова Н.И., Каня О.В. Фибробласты и их роль в развитии соединительной ткани // Сибирский медицинский журнал (Иркутск). - 2012. - Т. 110, № 3. - С. 8-12. Shurygina IA, Shurygin MG, Ayushinova NI, Kanya OV. (2012). Fibroblasts and their role in the development of connective tissue [Fibroblasty i ikh rol’ v razvitii soedi-nitel’noy tkani]. Sibirskiy meditsinskiy zhurnal (Irkutsk), 110 (3), 8-12.
6. Flavell S.J., Hou T.Z., Lax S., Filer A.D., Salmon M., Buckley C.D. (2008). Fibroblasts as novel therapeutic targets in chronic inflammation Br J Pharmacol, 153, 241-246.
7. Imamura Y., Scott I.C., Greenspan D.S. (2000). The pro-alpha3(V) collagen chain. Complete primary structure, expression domains in adult and developing tissues, and comparison to the structures and expression domains of the other types V and XI procollagen chains. J Biol Chem, 275 (12), 8749-8759.
8. Katada J., Saito H., Ohashi A. (2005). Significance of cyclooxygenase-2 induced via p38 mitogen-activated protein kinase in mechanical stimulus-induced peritoneal adhesion in mice. J Pharmacol Exp Ther, 313 (1), 286-292.
9. Musso O., Rehn M., Saarela J., Théret N., Liétard J., Hintikka, Lotrian D., Campion J.P., Pihlajaniemi T., Clément B. (1998). Collagen XVIII is localized in sinusoids and basement membrane zones and expressed by hepatocytes and activated stellate cells in fibrotic human liver. Hepatology, 28, 98-107.
10. Sumiyoshi H., Kitamura H., Matsuo N., Tatsukawa S., Ishikawa K., Okamoto O., Fujikura Y., Fujiwara S., Yoshioka H. (2012). Transient expression of mouse pro-a3(V) collagen gene (Col5a3) in wound healing. Connect Tissue Res, 53 (4), 313-317. doi: 10.3109/03008207.2011.653061.
Review
For citations:
Shurygina I.A., Shurygin M.G., Rodionova L.V., Chepurnykh E., Ayushinova N.I. EXPRESSION OF COLLAGENS IN THE DAMAGE AREA AT ABDOMINAL ADHESIONS. Acta Biomedica Scientifica. 2017;2(6):188-192. (In Russ.) https://doi.org/10.12737/article_5a0a90604689c9.94438754
Views:
950
.png)
Email this article 
