Sequencing of a fragment of the leptin gene in adolescents with different weight status
https://doi.org/10.29413/ABS.2023-8.4.10
Abstract
Background. Obesity is a significant social problem among the population of the world. The leptin gene (LEP) is currently considered as a potential candidate gene influencing metabolic disorders associated with predisposition to overweight and obesity. Leptin plays an important role in body weight homeostasis by influencing food intake and energy expenditure and maintaining constant energy stores. A defect in the leptin gene may be one of the causes of obesity and, as a result, of various obesity-associated pathologies.
The aim of the study. To search for single-nucleotide polymorphisms (SNP) of the leptin gene in adolescents with different weight status.
Methods. The study involved 20 adolescents aged 11–17 years with normal body weight and overweight/obesity. Research methods: assessment of clinical status with anthropometry; Sanger sequencing of the leptin gene fragment localized in the intron of this gene – (5’-AGCCTTGTTTTCATCATCTGGA, 3’-TGGGAGGAATCGCTCTCAGA). We also carried out bioinformatic processing of sequencing results.
Results. As a result of the study, the optimal conditions for amplification of the 891 bps leptin gene region were selected for the above mentioned primer pair of the LEP gene (s16_L891, s16_R891). Based on the results of sequencing, 45 single nucleotide substitutions of the LEP gene were identified, including 23 single nucleotide substitutions which were not previously registered in GenBank. In the group of adolescents with overweight and obesity, 14 unregistered single nucleotide substitutions of the LEP gene and 13 registered SNPs were identified in the GenBank database. In the group of adolescents with normal body weight, these SNPs were not found.
About the Authors
T. A. Bairova
Scientific Сentre for Family Health and Human Reproduction Problems
Russian Federation
Russian Federation
Tatyana A. Bairova – Dr. Sc. (Med.), Head of the Laboratory of Personalized Medicine
Timiryazeva str. 16, Irkutsk 664003
O. A. Ershova
Scientific Сentre for Family Health and Human Reproduction Problems
Russian Federation
Russian Federation
Oksana A. Ershova – Cand. Sc. (Biol.), Research Officer at the Laboratory of Personalized Medicine
Timiryazeva str. 16, Irkutsk 664003
A. Yu. Sambyalova
Scientific Сentre for Family Health and Human Reproduction Problems
Russian Federation
Russian Federation
Alexandra Yu. Sambyalova – Junior Research Officer at the Laboratory of Personalized Medicine
Timiryazeva str. 16, Irkutsk 664003
E. V. Belyaeva
Scientific Сentre for Family Health and Human Reproduction Problems
Russian Federation
Russian Federation
Elena V. Belyaeva – Cand. Sc. (Biol.), Research Officer at the Laboratory of Personalized Medicine
Timiryazeva str. 16, Irkutsk 664003
V. V. Sinkov
Scientific Сentre for Family Health and Human Reproduction Problems
Russian Federation
Russian Federation
Viacheslav V. Sinkov – Cand. Sc. (Med.), Senior Research Officer at the Laboratory of Epidemiologically and Socially Significant Infections
Timiryazeva str. 16, Irkutsk 664003
L. V. Rychkova
Scientific Сentre for Family Health and Human Reproduction Problems
Russian Federation
Russian Federation
Lyubov V. Rychkova – Dr. Sc. (Med.), Corresponding Member of the RAS, Director
Timiryazeva str. 16, Irkutsk 664003
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Review
For citations:
Bairova T.A., Ershova O.A., Sambyalova A.Yu., Belyaeva E.V., Sinkov V.V., Rychkova L.V. Sequencing of a fragment of the leptin gene in adolescents with different weight status. Acta Biomedica Scientifica. 2023;8(4):92-100. https://doi.org/10.29413/ABS.2023-8.4.10
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