Functional characteristics of tissue equivalents of various tissues using porous-permeable incubators of titanium nickelide
Abstract
Pancreas cages form a functional fabric and allocate hormonal substances necessary for indemnification of the lost structures of a pancreas of an organism. And, at input in an organism of ß-cells deprived of a substrate, life cycle transplanted xenogeneic cages considerably decreases under the influence of immune factors, during too time of a cage in an incubator function longer time, promoting construction of spatial architecture of new gland. The given methodology is effective in diabetes treatment as the transplanted cages it is very thin react to allocation of insulin depending on a condition homeostatic organism systems that is an actual problem at introduction of injections of insulin. Results of researches show that incubators of titanium nickelide are highly effective for their use as biocompatible matrixes. Features incubator spaces, and also a material titanium nickelide the titan promote creation fabric biosystems from various cages of an organism and to considerable prolongation of functional activity transplantation cages that accordingly conducts to increase in period of validity of therapeutic effect. Investigation have shown the formation of specific tissues in porous-permeable incubators of titanium nickelide for different cell populations. Allogeneic bone marrow cells immobilized in incubators of titanium nickelide possess antitumor (25%) and significant antimetastatic (45%) effect. The study of morphological parameters immunocompetent organs showed that administration of allogeneic bone marrow cells can decrease thymic regression, reduces splenomegaly in animals with transplantable tumors. Hepatocytes were implanted in porous materials of titanium nickelide mice with CCl4-induced hepatitis provided significant antitoxic effect. Obtained initial results promising pancreatic tissue-engineering in complex treatment of diabetes. It is noted that the use of incubators permeable porous nickel-titanium increase therapeutic effect and significantly prolongs effect of transplanted cells, as opposed their injecting.
About the Authors
O. V. Kokorev
Research Institute of Medical Shape Memory Materials and Implants
Russian Federation
Russian Federation
V. N. Khodorenko
Research Institute of Medical Shape Memory Materials and Implants
Russian Federation
Russian Federation
G. T. Dambaev
Siberian State Medical University
Russian Federation
Russian Federation
V. E. Gunther
Research Institute of Medical Shape Memory Materials and Implants
Russian Federation
Russian Federation
References
1. Барышников А.Ю. Взаимоотношение опухоли и иммунной системы организма // Практическая онкология. - 2003. - Т. 4, № 3. - С. 127-130
2. Бенч Л., Джонс Д. Биоматериалы, искусственные органы и инжиниринг тканей. - М.: Техносфера, 2007. - 304 с
3. Гюнтер В.Э., Ходоренко В.Н., Чекалкин Т.Л. и др. Медицинские материалы и имплантаты с памятью формы // Медицинские материалы с памятью формы / Под ред. В.Э. Гюнтера. - Томск: Изд-во МИЦ, 2011. -Т. 1. - 534 с
4. Дамбаев Г.Ц., Гюнтер В.Э., Зиганьшин Р.В. и др. Имплантаты с памятью формы в хирургии // Медицинские материалы с памятью формы / Под ред. В.Э. Гюнтера. - Томск: Изд-во МИЦ, 2012. - Т. 11. - 398 с
5. Имплантат для хирургического лечения заболеваний внутренних органов: Пат. 2143867 Рос. Федерация / Дамбаев Г.Ц., Гюнтер В.Э., Ходоренко В.Н., Загребин Л.В., Смольянинов Е.С., Чердынцева Н.В., Ясенчук Ю.Ф., Кокорев О.В. - 2000. - Бюл. № 1
6. Материалы с памятью формы в медицине / Под ред. В.Э. Гюнтера. - Томск: Изд-во «НПП МИЦ», 2014. - 342 с
7. Материалы с памятью формы и новые медицинские технологии / Под ред. В.Э. Гюнтера. - Томск: Изд-во «НПП МИЦ», 2010. - 360 с
8. Носитель клеточных культур искусственных внутренних органов: Патент 2191607, Рос. Федерация / Гюнтер В.Э., Дамбаев Г.Ц., Ходоренко В.Н., Загребин Л.В., Хлусов И.А., Ясенчук Ю.Ф. - 2002. - Бюл. № 30
9. Подольцева Э.И. Реакция «трансплантат против опухоли» - перспективный метод иммунотерапии злокачественных новообразований // Практическая онкология. - 2003. - Т. 4, № 3. - С. 175-182
10. Прохоров А.В., Третьяк С.И., Горанов В.А, Маркелов Д.В. Свободная трансплантация культуры ß-клеток в лечении экспериментального сахарного диабета // Актуальные вопросы современной медицины: Материалы Юбилейной научн. конф., посв. 80-летию БГМУ / Под ред. С.Л. Кабака. - Минск: БГМУ 2001. - Ч. II. - С. 91-93
11. Шумаков В.И., Блюмкин В.Н., Скалецкий Н.Н., Шальнев Б.И. и др. Трансплантация островковых клеток поджелудочной железы. - М.: Канон, 1995. - 383 с
12. Bettinger C.J., Kulig K.M., Vacanti J.P. et al. (2009). Nanofabricated collagen-inspired synthetic elastomers for primary rat hepatocyte culture. Tissue Engineering Part A., 15 (6), 1321-1329
13. Bokhari M., Carnachan R.J., Cameron N.R., Przyborski S.A. (2007). Culture of HepG2 liver cells on three dimensional polystyrene scaffolds enhances cell structure and during toxicological challenge. J. Anatomy., 21, 567-576.
14. Griffith L.G., Naughton G. (2002). Tissue engineering-current challenges and expanding opportunities. Science, 295, 1009-1114.
15. Gunther V.E. (ed.) (2011). Shape memory biomaterials and implants: Proceedings of international conference, 220-222
16. Kuo C.K., Li W.J., Mauck R.L. et al. (2006). Cartilage tissue engineering: its potential and uses. Curr. Opin. Rheumatol., 18, 64-73.
17. Lacy P.E. (2009). Islet transplantation in diabetes mellitus. Diabetes, 111 (3), 1-9.
18. Langer R., Vacanti J.P. (1993). Tissue engineering. Science, 260, 920-926.
19. Rose FR, Oreffo RO (2002). Bone tissue engineering: hope vs. hype. Biochem. Biophys. Res. Commun., 292, 1-7.
20. Rosenberg S.A., Yang J.C., Restifo N.P. (2004). Cancer immunotherapy: moving beyond current vaccines. Nat. Med, 10 (9), 909-915.
21. Sharma B., Elisseeff J.H. (2004). Engineering structurally organized cartilage and bone tissues. Ann. Biomed. Eng., 32, 148-159.
22. Vacanti J.P., Langer R. (1999). Tissue engineering: the design and fabrication of living replacement devices for surgical reconstruction and transplantation. Lancet, 354, 32-39.
Review
For citations:
Kokorev O.V., Khodorenko V.N., Dambaev G.T., Gunther V.E. Functional characteristics of tissue equivalents of various tissues using porous-permeable incubators of titanium nickelide. Acta Biomedica Scientifica. 2015;(2):73-79. (In Russ.)
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