Allelic polymorphism of atoxigenic Vibrio Cholerae strains of housekeeping genes isolated in Siberia and Far East
Abstract
There were atoxigenic Vibrio cholerae strains housekeeping genes analyzed. There were studied 20 isolates, in which nine sequence types have determined and five of which have described as unique. There were clustersformed as belonging to serogroup: first cluster includes RO-variantstrains and one O139 serogroup strain of V. cholerae, second - all O1 serogroup V. cholerae strains. There are sequence types associated with place and time isolation strains in some cases, although differentiation of the strains, which are formed the dominant sequence type strains group have no spatial and temporal characteristics associated.
About the Authors
E. G. Goldapel
Irkutsk Antiplague Research Institute of Siberia and Far East of Rospotrebnadzor
Russian Federation
Russian Federation
L. V. Mironova
Irkutsk Antiplague Research Institute of Siberia and Far East of Rospotrebnadzor
Russian Federation
Russian Federation
M. V. Afanas'ev
Irkutsk Antiplague Research Institute of Siberia and Far East of Rospotrebnadzor
Russian Federation
Russian Federation
S. V. Balakhonov
Irkutsk Antiplague Research Institute of Rospotrebnadzor
Russian Federation
Russian Federation
References
1. Achtman M. et al. Multilocus sequence typing as a replacement for serotyping in Salmonella enterica // PLoS Pathog. - 2012. - Vol. 8. - N 6. - e1002776. doi: 10.1371/ journal.ppat.1002776
2. Bogard R.W., Davies B.W., Mekalanos J.J. MetR-regulated Vibrio cholerae metabolism is required for virulence // MBio. - 2012. - Vol. 3, N 5. - e00236-12. doi: 10.1128/ mBio.00236-12
3. Boonsilp S. et al. A single multilocus sequence typing (MLST) scheme for seven pathogenic Leptospira species // PLoS Negl. Trop. Dis. - 2013. - Vol. 7, N 1. -e1954. doi: 10.1371/journal.pntd.0001954
4. Byun R. et al. Evolutionary relationships of pathogenic clones of Vibrio cholerae by sequence analysis of four housekeeping genes // Infect. Immun. - 1999. - Vol. 67, N 3. - P. 1116-1124.
5. Faruque S.M., Albert M.J., Mekalanos J.J. Epidemiology, genetics, and ecology of toxigenic Vibrio cholera // Microbiol. Mol. Biol. Rev. - 1998. - Vol. 62, N 4. -P. 1301-1314.
6. Larsen M.V. et al. Multilocus sequence typing of total-genome-sequenced bacteria // J. Clin. Microbi ol. - 2012. - Vol. 50, N 4. - Р. 1355-1361. doi: 10.1128/ JCM.06094-11
7. Maiden M.C. et al. Multilocus sequence typing: a portable approach to the identification of clones within populations of pathogenic microorganisms // Proc. Natl. Acad. Sci. USA. - 1998. - Vol. 95, N 6. - Р. 3140-3145.
8. Molecular epidemiology of O139 Vibrio cholerae·. mutation, lateral gene transfer, and founder flush / Garg P. [et al.] // Emerg. Infect. Dis. - 2003. - Vol. 9, N 7. - P. 810-814.
9. Moschioni M. et al. Sequence analysis of 96 genomic regions identifies distinct evolutionary lineages within CC156, the largest Streptococcus pneumoniae clonal complex in the MLST database // PLoS One. - 2013. - Vol. 8, N 4. - e61003. doi· 10.1371/journal. pone.0061003.
10. Octavia S. [et al.] Population structure and evolution of non-O1/non-O139 Vibrio cholerae by multilocus sequence typing // PLoS One. - 2013. - Vol. 8, N 6. - e65342. doi· 10.1371/journal.pone.0065342
11. World Health Organization. Cholera. 2012. Fact sheet N 107 [Электронный ресурс]. - Режим доступа· http://www.who.int/mediacentre/factsheets/fs107/ en/index.html.
12. Yeo M. et al. Multilocus sequence typing (MLST) for lineage assignment and high resolution diversity studies in Trypanosoma cruzi// PLoS Negl. Trop. Dis. - 2011. -Vol. 5, N 6. - e1049. doi· 10.1371/journal.pntd.0001049
Review
For citations:
Goldapel E.G., Mironova L.V., Afanas'ev M.V., Balakhonov S.V. Allelic polymorphism of atoxigenic Vibrio Cholerae strains of housekeeping genes isolated in Siberia and Far East. Acta Biomedica Scientifica. 2013;(6):132-135. (In Russ.)
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