HUMAN BONE MARROW AND ADIPOSE TISSUE DERIVED MESENCHYMAL STROMAL CELL INFLUENCE ON NEUROLOGICAL DEFICIT RECOVERY IN A MODEL OF SEVERE TRAUMATIC BRAIN INJURY IN RATS
Abstract
In the present study we characterized the effect of transplantation of bone marrow and adipose tissue MSCs in the model of brain injury in rats. This study was performed on Wistar rats (males and females in equal proportions with body weight of 250—270 g; n = 50). Traumatic brain injury was produced, by original spring-loaded, mechanism to precise dosing of impact force. Neurological disorders were assessed by Chen scale, a vertical grid test and a Morris water maze. MSC injections on day 1 were accompanied by a significant reduction in neurological deficit in compare with the control group. Adipose tissue derived MSCs were found to have a more pronounced effectiveness than MSCs obtained from bone marrow.
About the Authors
E. V. Polovnikov
Research Institute of Traumatology and Orthopedics
Russian Federation
Russian Federation
V. V. Stupak
Research Institute of Traumatology and Orthopedics
Russian Federation
Russian Federation
A. G. Samokhin
Research Institute of Traumatology and Orthopedics
Russian Federation
Russian Federation
I. A. Vasiliev
Research Institute of Traumatology and Orthopedics
Russian Federation
Russian Federation
O. B. Dobryakova
Research Institute of Traumatology and Orthopedics
Russian Federation
Russian Federation
E. Ya. Shevela
Research Institute of Clinical Immunology SB RAMS
Russian Federation
Russian Federation
E. R. Chernykh
Research Institute of Clinical Immunology SB RAMS
Russian Federation
Russian Federation
References
1. Григорян А.С. Влияние трансплантации мезенхимальных стволовых клеток на развитие посттравматических процессов в головном мозге крыс: автореф. дис.... канд. биолог. наук. — 21 с.
2. Останин А.А., Петровский Я.Л., Шевела Е.Я., Черных Е.Р. Мультиплексный анализ цитокинов, хемокинов, ростовых факторов, MMP-9 и TIMP-1, продуцируемых мезенхимальными стромальными клетками костного мозга, жировой ткани и плаценты человека // Клеточные технологии в биологии и медицине. — 2011. — № 1. — С. 29 — 38.
3. Южаков В.В., Конопляников А.Г., Цыб А.Ф., Рошаль Л.М. и др. Действие аутологичных мезенхимальных стволовых клеток на репаративные процессы в нервной ткани при диффузной травме головного мозга крыс // Стволовые клетки и перспектива их использования в здравоохранении: тез. докл. Всерос. междунар. науч. конф., 30 — 31 мая 2007 г. — М., 2007. — С. 18.
4. Borlongan C.V., Hadman M., Sanberg C.D., Sanberg P.R. Central nervous system entry of peripherally injected umbilical cord blood cells is not required for neuroprotection in stroke // Stroke. — 2004. — Vol. 35. — P. 2385 — 2389.
5. Chen J., Li Y., Wang L., Zhang Z. et al. Therapeutic benefit of intravenous administration of bone marrow stromal cells after cerebral ischemia in rats // Stroke. — 2001. — Vol. 32. — P. 1005—1011.
6. Chen J., Sanberg P.R., Li Y., Wang L. et al. Intravenous administration of human umbilical cord blood reduces behavioral deficits after stroke in rats // Stroke. — 2001. — Vol. 32. — P. 2682 — 2688.
7. Corti S., Locatelli F., Strazzer S., Guglieri M. et al. Neuronal Generation from Somatic Stem Cells: Current Knowledge and Perspectives on the Treatment of Acquired and Degenerative Central Nervous System Disorders // Current Gene Therapy. — 2003. — Vol. 3. — P. 247 — 272.
8. Nakada А., Fukuda S., Ichihara S., Sato T. et al. Regeneration of Central Nervous Tissue Using a Collagen Scaffold and Adipose-Derived Stromal Cells // Cells tissues organs. — 2009. — Vol. 190, N 6. — P. 256 — 258.
9. Parr A.M., Tator C.H., KeAting A. Bone-marrow-derived mesenchymal stromal cells for the repair of central nervous system injury // Bone marrow transplantation. — 2007. — Vol. 40. — P. 609 — 619.
Review
For citations:
Polovnikov E.V., Stupak V.V., Samokhin A.G., Vasiliev I.A., Dobryakova O.B., Shevela E.Ya., Chernykh E.R. HUMAN BONE MARROW AND ADIPOSE TISSUE DERIVED MESENCHYMAL STROMAL CELL INFLUENCE ON NEUROLOGICAL DEFICIT RECOVERY IN A MODEL OF SEVERE TRAUMATIC BRAIN INJURY IN RATS. Acta Biomedica Scientifica. 2012;(3(2)):301-304. (In Russ.)
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