Study of the effect of a new antiadhesive agent on peripheral blood indices (experimental study)

Abstract

Adhesive process in the abdominal cavity was simulated in Wistar rats, which were divided into two groups: the main one - simulation of adhesive process in the abdominal cavity and introduction of 3 ml of saline into the abdominal cavity; the controls - simulation of adhesive process in the abdominal cavity and introduction of 3 ml of new antiadhesive preparation. The mechanism of drug action bases on the ability to inhibit the growth of connective tissue in the adhesion area by effecting the activity of mitogen-activated protein kinases. Examination of peripheral blood indices was performed at eight time points during the period from 2 hours to 28 days. The concentration of red blood cells in the peripheral blood in both main and control groups decreased moderately in the early postoperative period (2 hours, 6 hours and 12 hours), and this rate was significantly lower in the control group as compared to the main one by 12 hours term. Later the index restored to normal. Hemoglobin concentration in the peripheral blood was higher in the main group than in the control one, the differences were significant in 2 hours, 3 days, 28 days. The level of hematocrit in the main group was higher than that in the controls during the entire follow-up period; the difference was maximal to the final term of experiment (28 days). The level of leukocytes in peripheral blood of the main group rats did not exceed the normal value for the given species of animals throughout the follow-up period. The number of eosinophils in the peripheral blood of the rodents of both groups did not differ at every period of survey. Thus, the introduction of the drug reduced the severity of leukocyte response to injury, improvement in postoperative hemoglobin and hematocrit levels.