Influence of the Pentaphylloides fruticosa l. liniment on TBA-active products and antioxidant status in wound injuries associated with experimental diabetes mellitus

Abstract

The aim of the work was to evaluate the influence of the linimentfrom Pentaphylloides fruticosa L. on lipid peroxidation in rats with experimental diabetes mellitus accompanied by wound process. The liniment was developed with the use of NanoDeBee30 device. Experiments were carried out on white male Wistar rats. Experimental diabetes mellitus was simulated by a single intraperitoneal introduction of alloxan in a dose of 100 mg/kg. The skin injury was simulated by inflicting planar wounds 2.0 x 2.0 cm in size. 5 mg of the 5%-liniment from Pentaphylloides fruticosa was put on the wounds of the first experimental group once a day for 14 days. The second experimental group was treated by the preparation of comparison - balsamic liniment. The wounds of the control group were treated with glycerin-lanolin ointment. On the 7^th, 14^th and 21^st day the concentration of TBA-active products in the blood serum was determined to estimate the intensity of lipid peroxidation processes; the contents of reduced glutathione and catalase activity were determined to evaluate the antioxidant status. The results of the experiments verify the marked curative effect of the linimentfrom Pentaphylloides fruticosa L. on the healing of the wounds on the background of experimental diabetes mellitus in rats