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Formation of Indoleacetic Acid by Enterbacteria Pathogenic for Human

https://doi.org/10.29413/ABS.2019-4.5.2

Abstract

Background. At present, the problem of plant colonization by bacteria pathogenic for human is of particular interest. Among the bacteria capable of polyhostality, species of the Enterobacteriaceae family, which includes both phytopathogenic and human pathogens, are of particular interest. Fruits and vegetables contaminated with such pathogens have been shown to be able to cause human intestinal infections. Being one of the ways of communication in the plantmicroorganism system, it is possible that the synthesis of indole acetic acid (IAA) by Enterobacteriaceae determines the growth rate of bacteria, contributing to more rapid colonization of the plant.
Aim: comparative study of the synthesis of IAA by different types of enterobacteria.
Methods. Eight types of enterobacteria were used in the study, seven of which were isolated from sick people, as well as the phytopathogen Pectobacterium carotovorum spp. carotovorum. The work was performed using microbiological and spectrophotometric research methods.
Results. The ability of the synthesis of IAA by opportunistic microorganisms belonging to the Enterobacteriaceae family was studied. We found that the majority of the studied strains synthesize IAA. Studies on the effect on the synthesis of IAA, the introduction into the nutrient medium of tryptophan and its enrichment with glucose, as well as the variation of the temperature regime of the cultivation of bacteria were carried out.

About the Authors

A. L. Turskaya
Siberian Institute of Plant Physiology and Biochemistry SB RAS
Russian Federation

Cand. Sc. (Biol.), Research Officer at the Laboratory of Plant-Microbial Interactions

Lermontov str. 132, Irkutsk 664033, Russian Federation



L. A. Belovezhets
A.E. Favorsky Irkutsk Institute of Chemistry SB RAS
Russian Federation

Cand. Sc. (Biol.), Research Officer at the Group of Pharmaceutical Development

Favorsky str. 1, Irkutsk 664033, Russian Federation



T. E. Putilina
Siberian Institute of Plant Physiology and Biochemistry SB RAS
Russian Federation

Leading Engineer at the Laboratory of Physicoсhemical Research Methods

Lermontov str. 132, Irkutsk 664033, Russian Federation



V. A. Bybin
Siberian Institute of Plant Physiology and Biochemistry SB RAS
Russian Federation

Cand. Sc. (Biol.), Leading Engineer at the Laboratory of Plant-Microbial Interactions

Lermontov str. 132, Irkutsk 664033, Russian Federation



A. V. Dukhanina
Scientific Centre for Family Health and Human Reproduction Problems
Russian Federation

Cand. Sc. (Biol.), Engineer at the Laboratory of Epidemiologically and Socially Significant Infections

Timiryazev str. 16, Irkutsk 664003, Russian Federation



Yu. A. Markova
Siberian Institute of Plant Physiology and Biochemistry SB RAS
Russian Federation

Dr. Sc. (Biol.), Head of the Laboratory of Plant-Microbial Interactions

Lermontov str. 132, Irkutsk 664033, Russian Federation



References

1. Markova YuA, Alekseenko AL, Kramarsky AV, Savilov ED. Plants as one of the links in the circulation chain of bacteria pathogenic for humans in the environment. Siberian Medical Journal (Irkutsk). 2012; 114(7): 11-14. (In Russ.)

2. Markova YuA, Dukhanina AV, Anganova EV, Belovezhets LA, Savilov ED. Contamination of foods of plant origin by pathogenic and opportunistic enterobacteria. Acta biomedica scientifica. 2012; (5): 268-270. (In Russ.)

3. Arthurson V, Sessitsch A, Jäderlund L. Persistence and spread of Salmonella enterica serovar Weltevreden in soil and on spinach plants. FEMS Microbiol. Let. 2011; 314(1): 67-74. doi: 10.1111/j.1574-6968.2010.02140.x

4. Kirzinger MW, Nadarasah G, Stavrinides J. Insights into cross-kingdom plant pathogenic bacteria. Genes (Basel). 2011; 2(4): 980-997. doi: 10.3390/genes2040980

5. Tsavkelova EA, Klimova SYu, Cherdyntseva TA, Netrusov AI. Microbial producers of plant growth stimulators and their practical use: A review. Prikladnaya biokhimiya i mikrobiologiya. 2006; 42(2): 117-126. doi: 10.1134/S0003683806020013

6. Bianco C, Imperlini E, Calogero R, Senatore B, Pucci P, Defez R. Indole-3-acetic acid regulates the central metabolic pathways in Escherichia coli. Microbiology. 2006; 152(8): 2421-2431. doi: 10.1099/mic.0.28765-0

7. Tyler HL, Triplett EW. Plants as a habitat for beneficial and/or human pathogenic bacteria. Annu Rev Phytopathol. 2008; 46: 53-73. doi: 10.1146/annurev.phyto.011708.103102

8. Katsy EI. Molecular genetics of the associative interactions between bacteria and plants. Current status and prospects of the studies. Moscow: Nauka; 2007. (In Russ.)

9. Kravchenko LV, Azarova TS, Makarova NM, Tikhonovich IA. The role of tryptophan in root exometabolites for phytostimulating activity of rhizobacteria. Microbiology. 2004; 73(2): 195-198. (In Russ.)

10. Celloto VR, Oliveira AJ, Gonçalves JE, Watanabe CS, Matioli G, Gonçalves RA. Biosynthesis of indole-3-acetic acid by new Klebsiella oxytoca free and immobilized cells on inorganic matrices. Scientific World J. 2012; 2012: 495970. doi: 10.1100/2012/495970

11. Glantz S. Primer of biostatistics. Trans Danilov YuA. Moscow: Praktika; 1998. (In Russ.)

12. Duca D, Lorv J, Patten CL, Rose D, Glick BR Indole-3-acetic acid in plant-microbe interactions. Antonie Van Leeuwenhoek. 2014; 106 (1): 85-125. doi 10.1007/s10482-013-0095-y

13. Khare E, Arora NK. Effect of indole-3-acetic acid (IAA) produced by Pseudomonas aeruginosa in suppression of charcoal rot disease of chickpea. Curr Microbiol. 2010; 61(1): 64-68. doi: 10.1007/s00284-009-9577-6

14. Kim YC, Leveau J, McSpadden Gardener BB, Pierson EA, Pierson LS 3rd, Ryu CM. The multifactorial basis for plant health promotion by plant-associated bacteria. Appl Environ Microbiol. 2011; 77(5): 1548-1555. doi: 10.1128/AEM.01867-10

15. Spaepen S, Vanderleyden J, Remans R. Indole-3-acetic acid in microbial и microorganism-plant signaling. FEMS Microbiol Rev. 2007; 31(4): 425-448. doi: 10.1111/j.1574-6976.2007.00072.x

16. Ona O, Van Impe J, Prinsen E, Vanderleyden J. Growth and indole-3-acetic acid biosynthesis of Azospirillum brasilense Sp245 is environmentally controlled. FEMS Microbiol Lett. 2005; 246(1): 125-132. doi:10.1016/j.femsle.2005.03.048


Review

For citations:


Turskaya A.L., Belovezhets L.A., Putilina T.E., Bybin V.A., Dukhanina A.V., Markova Yu.A. Formation of Indoleacetic Acid by Enterbacteria Pathogenic for Human. Acta Biomedica Scientifica. 2019;4(5):14-18. (In Russ.) https://doi.org/10.29413/ABS.2019-4.5.2

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