GENETIC ANALYSIS OF HOKKAIDO HANTAVIRUS AMONG MYODES RUFOCANUS IN THE BAIKAL LAKE AREA
Abstract
Hokkaido hantavirus (HOKV) identified originally in the grey red-backed vole (Myodes rufocanus) in Hokkaido, Japan. Subsequent studies showed different genetic lineages of HOKV in Sakhalin, Buryatia and Far Eastern regions of Russia and in China. Tissuesfrom 68 arvicolid rodents, captured in regions south and west of Baikal Lake, were initially tested for hantaviral antigen by ELISA, and tissues from antigen-positive rodents were analyzed for hantavirus RNA by RT-PCr. Taxonomic identification of host species was based on phylogenetic analysis of partial cytochrome b gene sequences. Hantavirus Land S-segment sequences were detected in two antigen-positive M. rufocanus, from the Tunka region of Buryatia Republic (south side) and the Olhon region of Irkutsk Oblast (west side). Sequence analysis showed that the newfound hantavirus strains, designated Baikal and Siberia, represented genetic variants of HOKV Previously unknown genetic variant designated Siberia was identified in M. rufocanus captured in Olhon region. Second genetic variant from Tunka region, designated Baikal, was closely related to previously described hantavirus strain from the same region. Alignment and comparison of the nucleotide and amino acid sequences showed intra-strain differences of 18,4 % and 5,3 % for the L segment and 17,4 % and 3,5 % for the S segment, respectively. Sequence divergence from geographically distant HOKV strains were 17,4-21,5 % and 3,9-6,8 % for the L segment and 15,2-17,0 % and 3,3-4,0 % for the S segment, respectively. Phylogenetic analysis, based on a 346-nucleotide region of the L segment, revealed four lineages represented by previously reported variants from Japan and Sakhalin (strains Kitahiyama128L/2008, Tobetsu35L/2010 and Sakhalin99L/1998), Shkotovo in Far-Eastern Russia (strain Khekhtsir37L/2002) and the new variants from Baikal Lake. Analysis of the N protein, coding by the S segment, identified specific amino acid signatures for YJRV of Lys5, Arg26, Val/Ile68, Val/Ala9 He262, Pro283. Conclusions: HOKV is widespread across the geographic range of its arvicolid rodent reservoir host.
About the Authors
L. N. Yashina
State Research Center of Virology and Biotechnology «Vector»
Russian Federation
Russian Federation
G. A. Danchinova
Scientific Center of Family Health and Human Reproduction Problems SB RAMS
Russian Federation
Russian Federation
S. V. Seregin
State Research Center of Virology and Biotechnology «Vector»
Russian Federation
Russian Federation
M. A. Khasnatinov
Scientific Center of Family Health and Human Reproduction Problems SB RAMS
Russian Federation
Russian Federation
R. Yanagihara
University of Hawaii at Manoa
Russian Federation
Russian Federation
References
1. Данчинова Г.А., Яшина Л.Н., Чапоргина Е.А., Хаснатинов М.А. и др. Хантавирусы на территории Прибайкалья // Дальневосточный журнал инфекционной патологии. 2008. № 13. С. 180-181.
2. Малкин А.Е., Мясников Ю.А., Рыльцева Е.В., Ткаченко Е.А. Ландшафтное районирование природных очагов ГЛПС в России // Медицинская паразитол. и паразитар. болезни. 1996. № 2. С. 27-32.
3. Ткаченко Е.А., Рыльцева Е.В., Мясников Ю.А., Иванов А.П. и др. Изучение циркуляции вируса геморрагической лихорадки с почечным синдромом среди мелких млекопитающих на территории СССР // Вопр. вирусол. 1987. № 32 (6). С. 709-715.
4. Яшина Л.Н., Слонова Р.А., Олейник О.В., Кузина И.И. и др. Новый генетический вариант вируса Пуумала в Приморье и его природный носитель, красно-серая полевка Clethrionomys rufocanus // Вопр. вирусол. 2004. № 49 (6). С. 34-37.
5. Brummer-Korvenkontio M., Henttonen H., Va-hery A. Hemorrhagic fever with renal syndrome in Finland: ecology and virology of nephropathia epidemica // Scand. J. Infect. Dis. 1982. Vol. 36. P. 88-91.
6. Dekonenko A., Yakimenko V., Ivanov A., Morozov V. et al. Genetic similarity of Puumala viruses found in Finland and western Siberia and of the mitochondrial DNA of their rodent hosts suggests a common evolutionary origin // Infect. Genet. Evol. 2003. Vol. 3. -P. 245-257.
7. Garanina S.B., Platonov A.E., Zhuravlev V.I., Murashkina A.N. et al. Genetic diversity and geographic distribution of hantaviruses in Russia // Zoonoses Public Health. 2009. Vol. 56. P. 297-309.
8. Kariwa H., Yashimatsu K., Sawabe J., Yokota E. et al. Genetic diversities of hantaviruses among rodents in Hokkaido, Japan and Far East Russia // Virus Res. 1999. -Vol. 59. P. 219-228.
9. Kariwa H., Yoshizumi S., Arikawa J., Yoshimatsu K. et al. Evidence for the existence of Puumala-related virus among Clethrionomys rufocanus in Hokkaido, Japan // Am. J. Trop. Med. Hyg. 1995. P. 222-227.
10. Kosoy M., Slonova R., Mills J., Mandel E. et al. Community structure and prevalence of hantavirus infection in rodents: a geographic division of the enzootic area in far eastern Russia // J. Vect. Ecol. 1997. Vol. 22. P. 52-63.
11. Kraus A.A., Kafteri M.J., Giese T., Ulrich R. et al. Differential antiviral response of endothelian cells after infection with pathogenic and nonpathogenic hantaviruses // J. Virol. 2004. Vol. 78. P. 6143-6150.
12. Mills J.N., Childs J.E., Ksiazek T.G., Peters C.J. Methods for trapping and sampling small mammals for virologic testing. U.S. Department of Health and Human Services, Center for Disease Control and Prevention. -Atlanta, 1995.
13. Plyusnin A., Vapalahti A., Ulfves K., Lehvaslaiho H. et al. Sequences of wild Puumala virus genes show a correlation of genetic variation with geographic origin of the strains // J. Gen. Virol. 1994. Vol. 75. P. 405-409.
14. Plyusnina A., Laakkonen J., Niemimaa J., Nemirov K. et al. Genetic analysis of hantaviruses carried by Myodes and Microtus rodents in Buryatia // Virol. J. 2008. Vol. 54. P. 4-10.
15. Sanada T., Seto T., Ozaki Y., Saasa N. et al. Isolation of Hokkaido virus, genus Hantavirus, using a newly established cell line derived from the kidney of the grey red-backed vole (myodes rufocanus bedfordiae) // J. Gen. Virol. 2012. P. 2237-2246.
16. Schmaljohn C., Hjelle B. Hantaviruses: a global disease problem // Emerg. Infect. Dis. 1987. Vol. 3. -P. 95-104.
17. Sironen T., Vaheri A., Plyusnin A. Molecular evolution of Puumala hantavirus // J. Virol. 2001. Vol. 75. P. 11803-11810.
18. Song K.J., Baek L.J., Moon S., Ha S.J. et al. Muju virus, a novel hantavirus harboured by the arvicolid rodent Myodes regulus in Korea // J. Gen. Virol. 2007. -Vol. 88. P. 3121-3129.
19. Tamura K., Dudley J., Nei M., Kumar S. MEGA4: Molecular evolutionary genetic analysis (MEGA) software version 4.0 // Mol. Biol. Evol. 2007. Vol. 24. -P. 1596-1599.
20. Vapalahti O., Mustonen J., Lundkvist A., Hent-tonen H. et al. Hantavirus infections in Europe // Lancet Infect. Dis. 2003. Vol. 3. P. 653-661.
21. Zhang Y.Z., Zou Y., Yan Y.Z., Hu G.W. et al. Detection of phylogenetically distinct Puumala-like viruses from red-grey vole Clethrionomys rufocanus in China // J. Med. Virol. 2007. Vol. 79. P. 1208-1218.
Review
For citations:
Yashina L.N., Danchinova G.A., Seregin S.V., Khasnatinov M.A., Yanagihara R. GENETIC ANALYSIS OF HOKKAIDO HANTAVIRUS AMONG MYODES RUFOCANUS IN THE BAIKAL LAKE AREA. Acta Biomedica Scientifica. 2013;(4):147-152. (In Russ.)
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