actabiomedicaActa Biomedica ScientificaActa Biomedica Scientifica2541-94202587-9596Scientific Centre for Family Health and Human Reproduction Problemsactabiomedica-946Research ArticleЭКСПЕРИМЕНТАЛЬНЫЕ ИССЛЕДОВАНИЯ В БИОЛОГИИ И МЕДИЦИНЕEXPERIMENTAL RESEARCHES IN BIOLOGY AND MEDICINEЦИТОТОКСИЧЕСКАЯ АКТИВНОСТЬ ДЕНДРИТНЫХ КЛЕТОК ПРОТИВ АКТИВИРОВАННЫХ CD4+ И CD8 Т-ЛИМФОЦИТОВ В КУЛЬТУРЕ IN VITROCYTOTOXIC ACTIVITY OF DENDRITIC CELLS AGAINST ACTIVATED CD4+ AND CD8+T-LYMPHOCYTES IN THE CULTURE IN VITROТыриноваТ. В.TyrinovaT. V.tyrinova@bk.ruЛеплинаО. Ю.LeplinaO. Yu.oleplina@mail.ruТихоноваМ. А.TihonovaM. A.noemail@neicon.ruОстанинА. А.OstaninA. A.noemail@neicon.ruЧерныхЕ. Р.ChernykhE. R.bmt-novosibirsk@mail.ruНИИ клинической иммунологии СО РАМНInstitute of Clinical Immunology SB RAMS20122805201203(2)327331Copyright © Тыринова Т.В., Леплина О.Ю., Тихонова М.А., Останин А.А., Черных Е.Р., 20122012Тыринова Т.В., Леплина О.Ю., Тихонова М.А., Останин А.А., Черных Е.Р.Tyrinova T.V., Leplina O.Y., Tihonova M.A., Ostanin A.A., Chernykh E.R.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.actabiomedica.ru/jour/article/view/946

Целью настоящего исследования явилась сравнительная оценка цитотоксической активности IFNα-индуцированных дендритных клеток (ИФН-ДК) и IL-4-индуцированных ДК (ИЛ4-ДК) против активированных в алло-СКЛ CD4+ и CD8+ Т-лимфоцитов. Показано, что в культурах с ИФН-ДК доля AnnexinV+/PI'клеток, соответствующих ранней стадии апоптоза, среди CD4+Т-лимфоцитов была ниже, чем в культурах с ИЛ4-ДК. В то же время относительное количество AnnexinV+/PI+клеток (поздняя фаза апоптоза/некроз) среди CD4+Т-клеток после активации аллогенными ИФН-ДК и ИЛ4-ДК возрастало в равной степени. Продемонстрировано, что ИФН-ДК обладали сходной c ИЛ4-ДК способностью индуцировать апоптоз в CD3+CD8+Т-клетках.

The aim of the present study was a comparative assessment of the cytotoxic activity of IFNα-induced dendritic cells (IFN-DCs) and IL-4-induced DCs (IL4-DCs) against allo-MLC- activated CD4+ and CD8+T-lymphocytes. It was shown the level of AnnexinV+/PI- cells corresponding to early apoptosis among CD4+T-lymphocytes was lower in cultures with IFN-DCs compared with IL4-DCs. At the same time the relative number of AnnexinV+/ PI+ cells (late phase of apoptosis/necrosis) among CD4+T-lymphocytes activated by allogenic IFN-DCs and IL4-DCs increased in an equal degree. It was demonstrated that IFN-DCs possesed similar ability to induce apoptosis of CD3+CD8+T-lymphocytes.

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The authors declare that there are no conflicts of interest present.