actabiomedicaActa Biomedica ScientificaActa Biomedica Scientifica2541-94202587-9596Scientific Centre for Family Health and Human Reproduction Problemsactabiomedica-899Research ArticleКЛИНИЧЕСКАЯ МЕДИЦИНАCLINICAL MEDICINEДИНАМИКА ПОВЕРХНОСТНОЙ ЭКСПРЕССИИ ТРИГГЕРНОГО РЕЦЕПТОРА, ЭКСПРЕССИРУЕМОГО МИЕЛОИДНЫМИ КЛЕТКАМИ-1 (TREM-1) НА РАЗЛИЧНЫХ СУБПОПУЛЯЦИЯХ МОНОЦИТОВ В РАННЕМ ПОСЛЕОПЕРАЦИОННОМ ПЕРИОДЕ ПРЯМОЙ РЕВАСКУЛЯРИЗАЦИИ МИОКАРДАDYNAMICS OF SURFACE EXPRESSION OF TRIGGER RECEPTOR, EXPRESSED BY MYELOID CELLS-1 (TREM-1) ON VARIOUS SUBPOPULATIONS OF MONOCYTES IN EARLY POSTOPERATIVE PERIOD OF DIRECT REVASCULARIZATION OF MYOCARDIUMМатвееваВ. Г.MatveyevaV. G.matveeva_vg@mail.ruГоловкинА. С.GolovkinA. S.golovkin_a@mail.ruЧерноваМ. Н.ChernovaM. N.chernova.maria.n@mail.ruШукевичД. Л.ShukevichD. L.noemail@neicon.ruГригорьевЕ. В.GrigoryevE. V.grigorievev@mail.ruНИИ комплексных проблем сердечно-сосудистых заболеваний СО РАМНResearch Institute of Complex Problems of Cardiovascular Diseases SB RAMS20122805201203(2)116120Copyright © Матвеева В.Г., Головкин А.С., Чернова М.Н., Шукевич Д.Л., Григорьев Е.В., 20122012Матвеева В.Г., Головкин А.С., Чернова М.Н., Шукевич Д.Л., Григорьев Е.В.Matveyeva V.G., Golovkin A.S., Chernova M.N., Shukevich D.L., Grigoryev E.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.actabiomedica.ru/jour/article/view/899

Исследована поверхностная экспрессия триггерного рецептора, экспрессируемого миелоидными клетками-1 (TREM-1) на различных популяциях моноцитов до проведения операции прямой реваскуляризации миокарда в условиях искусственного кровообращения (ИК) и в раннем послеоперационном периоде. Нами зарегистрировано, что субпопуляциям моноцитов соответствует различная экспрессия TREM-1 на поверхности, при этом наибольший уровень обнаружен на моноцитах CD14hiCD16+. К 1-м суткам послеоперационного периода происходит повышение поверхностной экспрессии. TREM-1 на всех субпопуляциях моноцитов, свидетельствуя об увеличении их провоспалительного потенциала.

We studied surface expression of trigger receptor, expressed by myeloid cells-1 (TREM-1) on various subpopulations of monocytes before direct revascularization of myocardium with artificial blood flow and in early postoperative period. We registered that various expression of TREM-1 on the surface corresponds to subpopulations of monocytes, and the highest level is observed on CD14hiCD16+ monocytes. By the 1st day of postoperative period elevation of surface expression of TREM-1 occurs on all subpopulations of monocytes, testifying to enlargement of their proinflammatory potential.

субпопуляции моноцитовСВОTREM-1TREM-1subpopulations of monocytessystemic inflammatory responseReferencesГусев Е.Ю., Черешнев В.А., Юрченко Л.Н.. Системное воспаление с позиции теории типового патологического процесса // Цитокины и воспаление. - 2007. - T. 6, Вып. 4. - С. 9-21.Гусев Е.Ю., Черешнев В.А., Юрченко Л.Н.. Системное воспаление с позиции теории типового патологического процесса // Цитокины и воспаление. - 2007. - T. 6, Вып. 4. - С. 9-21.Bleharski J.R., Kiessler V., Buonsanti C. A role for triggering receptor expressed on myeloid cells-1 in host defense during the early-induced and adaptive phases of the immune response // J. Immunol. -2003. - Vol. 170, N 7. - Р. 3812-3818.Bleharski J.R., Kiessler V., Buonsanti C. A role for triggering receptor expressed on myeloid cells-1 in host defense during the early-induced and adaptive phases of the immune response // J. Immunol. -2003. - Vol. 170, N 7. - Р. 3812-3818.Bone R.C., Balk R.A., Cerra F.B. Definitions for sepsis and organ failure and guidelines for the use of innovative therapies in sepsis. The ACCP/SCCM Consensus Conference Committee. American College of Chest Physicians/Society of Critical Care Medicine // Chest. -1992. - Vol. 101, N 6. -Р. 1644-1655.Bone R.C., Balk R.A., Cerra F.B. Definitions for sepsis and organ failure and guidelines for the use of innovative therapies in sepsis. The ACCP/SCCM Consensus Conference Committee. American College of Chest Physicians/Society of Critical Care Medicine // Chest. -1992. - Vol. 101, N 6. -Р. 1644-1655.Bouchon A., Dietrich J., Colonna M. Cutting edge: inflammatory responses can be triggered by TREM-1, a novel receptor expressed on neutrophils and monocytes // J. Immunol. - 2000. - Vol. 164, N 10. - Р. 4991 -4995.Bouchon A., Dietrich J., Colonna M. Cutting edge: inflammatory responses can be triggered by TREM-1, a novel receptor expressed on neutrophils and monocytes // J. Immunol. - 2000. - Vol. 164, N 10. - Р. 4991 -4995.Bouchon A., Facchetti F., Weigand M.A., Colonna M. TREM-1 amplifies inflammation and is a crucial mediator of septic shock // Nature. - 2001. -Vol. 410. - Р. 1103-1107.Bouchon A., Facchetti F., Weigand M.A., Colonna M. TREM-1 amplifies inflammation and is a crucial mediator of septic shock // Nature. - 2001. -Vol. 410. - Р. 1103-1107.Cros J., Cagnard N., Woollard K. Human CD-14dim monocytes patrol and sense nucleic acids and viruses via TLR7 and TLR8 receptors // Immunity. - 2010. - Vol. 33, N 3. - P. 375-386.Cros J., Cagnard N., Woollard K. Human CD-14dim monocytes patrol and sense nucleic acids and viruses via TLR7 and TLR8 receptors // Immunity. - 2010. - Vol. 33, N 3. - P. 375-386.Dower K., Ellis D.K., Saraf K. Innate Immune Responses to TREM-1 Activation: Overlap, Divergence, and Positive and Negative Cross-Talk with Bacterial Lipopolysaccharide // J. Immunol. - 2008. -Vol. 180. - Р. 3520-3534.Dower K., Ellis D.K., Saraf K. Innate Immune Responses to TREM-1 Activation: Overlap, Divergence, and Positive and Negative Cross-Talk with Bacterial Lipopolysaccharide // J. Immunol. - 2008. -Vol. 180. - Р. 3520-3534.Gibot S., Massin F., Alauzet C., Montemont C. et al. Effects of the TREM-1 pathway modulation during mesenteric ischemia-reperfusion in rats // Crit. Care Med. - 2008. - Vol. 36, N 2. - Р. 504-510.Gibot S., Massin F., Alauzet C., Montemont C. et al. Effects of the TREM-1 pathway modulation during mesenteric ischemia-reperfusion in rats // Crit. Care Med. - 2008. - Vol. 36, N 2. - Р. 504-510.Gibot S., Massin F., Marcou M. TREM-1 promotes survival during septic shock in mice // Eur. J. Immunol. - 2007. - Vol. 37. - Р. 456-466.Gibot S., Massin F., Marcou M. TREM-1 promotes survival during septic shock in mice // Eur. J. Immunol. - 2007. - Vol. 37. - Р. 456-466.Markus P. Radsak, Helmut R. Salih Hans-Georg. Rammensee, Hansjörg Schild Triggering Receptor Expressed on Myeloid Cells-1 in Neutrophil Inflammatory Responses: Differential Regulation of Activation and Survival // J. Immunol. - 2004. -Vol. 172. - Р. 4956-4963.Markus P. Radsak, Helmut R. Salih Hans-Georg. Rammensee, Hansjörg Schild Triggering Receptor Expressed on Myeloid Cells-1 in Neutrophil Inflammatory Responses: Differential Regulation of Activation and Survival // J. Immunol. - 2004. -Vol. 172. - Р. 4956-4963.Merino A., Buendia P., Martin-Malo A. Senescent CD14 + CD16+ Monocytes Exhibit Proinflammatory and Proatherosclerotic Activity // J. Immunol. - 2010. - Vol. 186. - P. 1809-1815.Merino A., Buendia P., Martin-Malo A. Senescent CD14 + CD16+ Monocytes Exhibit Proinflammatory and Proatherosclerotic Activity // J. Immunol. - 2010. - Vol. 186. - P. 1809-1815.Murakami Y., Akahoshi T., Hayashi I., Endo H. et al. Induction of triggering receptor expressed on myeloid cells 1 in murine resident peritoneal macrophages by monosodium urate monohydrate crystals // Arthritis Rheum. - 2006. -Vol. 54. - Р. 455-462.Murakami Y., Akahoshi T., Hayashi I., Endo H. et al. Induction of triggering receptor expressed on myeloid cells 1 in murine resident peritoneal macrophages by monosodium urate monohydrate crystals // Arthritis Rheum. - 2006. -Vol. 54. - Р. 455-462.Nahrendorf M., Swirski F.K., Aikawa E. The healing myocardium sequentially mobilizes two monocyte subsets with divergent and complementary functions // J. Exp. Med. - 2007. - Vol. 204. -P. 3037-3047.Nahrendorf M., Swirski F.K., Aikawa E. The healing myocardium sequentially mobilizes two monocyte subsets with divergent and complementary functions // J. Exp. Med. - 2007. - Vol. 204. -P. 3037-3047.Netea M.G., Azam T., Ferwerda G. Triggering receptor expressed on myeloid cells-1 (TREM-1) amplifies the signals induced by the NACHT-LRR (NLR) pattern recognition receptors // J. Leuk. Biol. - 2006. - Vol. 80. - Р. 1454-1461.Netea M.G., Azam T., Ferwerda G. Triggering receptor expressed on myeloid cells-1 (TREM-1) amplifies the signals induced by the NACHT-LRR (NLR) pattern recognition receptors // J. Leuk. Biol. - 2006. - Vol. 80. - Р. 1454-1461.Steppich B., Dayyani F., Gruber R. Selective mobilization of CD14 + CD16+ monocytes by exercise // Am. J. Physio.l Cell Physiol. - 2000. -Vol. 279. - P. 578-586.Steppich B., Dayyani F., Gruber R. Selective mobilization of CD14 + CD16+ monocytes by exercise // Am. J. Physio.l Cell Physiol. - 2000. -Vol. 279. - P. 578-586.Tallone T., Turconi G., Soldati G. Heterogeneity of Human Monocytes: An Optimized Four-Color Flow Cytometry Protocol for Analysis of Monocyte Subsets // J. of Cardiovasc. Trans. Res. - 2011. -Vol. 4. - P. 211-219.Tallone T., Turconi G., Soldati G. Heterogeneity of Human Monocytes: An Optimized Four-Color Flow Cytometry Protocol for Analysis of Monocyte Subsets // J. of Cardiovasc. Trans. Res. - 2011. -Vol. 4. - P. 211-219.Ziegler-Heitbrock L. The CD14+ CD16+ blood monocytes: their role in infection and inflammation // J. Leukocyte Biol. - 2007. - Vol. 81, N 3. - P. 584-592.Ziegler-Heitbrock L. The CD14+ CD16+ blood monocytes: their role in infection and inflammation // J. Leukocyte Biol. - 2007. - Vol. 81, N 3. - P. 584-592.

The authors declare that there are no conflicts of interest present.