actabiomedicaActa Biomedica ScientificaActa Biomedica Scientifica2541-94202587-9596Scientific Centre for Family Health and Human Reproduction Problemsactabiomedica-862Research ArticleЭКСПЕРИМЕНТАЛЬНЫЕ ИССЛЕДОВАНИЯ В БИОЛОГИИ И МЕДИЦИНЕEXPERIMENTAL RESEARCHES IN BIOLOGY AND MEDICINEСОВРЕМЕННЫЕ ПОДХОДЫ К МОЛЕКУЛЯРНОЙ ХАРАКТЕРИСТИКЕ ОПУХОЛЕЙ ПРОСТАТЫMODERN APPROACH TO MOLECULAR CHARACTERIZATION OF PROSTATE TUMORSХвостоваЕ. П.KhvostovaE. P.ivanovakatysha@mail.ruПустыльнякВ. О.PustylnyakV. O.noemail@neicon.ruКорбутА. И.KorbutA. I.noemail@neicon.ruОтпущенниковА. А.OtpuschennikovA. A.noemail@neicon.ruГуляеваЛ. Ф.GulyaevaL. F.noemail@neicon.ruКрыловаИ. Н.KrylovaI. N.noemail@neicon.ruФГБУ Институт молекулярной биологии и биофизики СО РАМН; Новосибирский государственный университетInstitute of Molecular Biology and Biophysics, Siberian Branch of Russian Academy of Medical Sciences; Novosibirsk State UniversityНовосибирский государственный университетNovosibirsk State UniversityЦентральная Клиническая Больница СО РАНCentral Clinical Hospital Siberian Branch of Russian Academy of Sciences20122805201203(1)8388Copyright © Хвостова Е.П., Пустыльняк В.О., Корбут А.И., Отпущенников А.А., Гуляева Л.Ф., Крылова И.Н., 20122012Хвостова Е.П., Пустыльняк В.О., Корбут А.И., Отпущенников А.А., Гуляева Л.Ф., Крылова И.Н.Khvostova E.P., Pustylnyak V.O., Korbut A.I., Otpuschennikov A.A., Gulyaeva L.F., Krylova I.N.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.actabiomedica.ru/jour/article/view/862

Задачей исследования являлось создание клеточной биосенсорной системы, основанной на факте, что стимулы, активирующие AR, приводят к перераспределению AR из цитоплазмы в ядро. Эти изменения могут быть измерены и использованы для диагностики и персонализованного лечения рака простаты. Были созданы стабильные клеточные линии с флуоресцентно помеченным AR. Для стабильной экспрессии клеток были использованы клетки HeLa, не экспрессирующие AR. Для трансфекции выбраны плазмидные конструкты, экспрессирующие химеры флуоресцентного белка с AR дикого типа или ART877A мутантом. Было исследовано изменение CFP-AR-YFP и CFP-ART877A-YFP в ядрах клеток HeLa при обработке стероидами разной концентрации. Кроме того, проведена оценка количества активированного рецептора в ядрах после воздействия стероидов.

The aim of the study was the development of a cell biosensor system based on the fact that AR activator stimuli lead to redistribution of AR from cytoplasm to the nucleus. These changes could be measured and used for the diagnostics and personalized treatment of prostate cancer patient. The stably transfected cell lines with fluorescently tagged AR were generated. For the stable cell expression the non AR expressing HeLa cell line was used. The constructs of chimeric fluorescent proteins wild type AR or mutant ART877A were used for the transfection. The changes of the CFP-AR-YFP and CFP-ART877A-YFP proteins in the nucleus of HeLa cells under different concentrations steroids treatment was investigated. Moreover, the quantity of activated receptor in the nucleus was carried.

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The authors declare that there are no conflicts of interest present.