actabiomedicaActa Biomedica ScientificaActa Biomedica Scientifica2541-94202587-9596Scientific Centre for Family Health and Human Reproduction Problems10.29413/ABS.2026-11.4.22actabiomedica-6337Research ArticleЭКСПЕРИМЕНТАЛЬНЫЕ ИССЛЕДОВАНИЯEXPERIMENTAL RESEARCHESЛучевые признаки деградации и морфологическая реакция мягких тканей на имплантаты Mg–2Zn–2Ga и титана в эксперименте in vivoRadiological signs of degradation and soft-tissue response to Mg–2Zn–2Ga and titanium implants in vivohttps://orcid.org/0009-0001-9077-6528КунижевК. А.KunizhevK. A.

Кунижев Кантемир Анатольевич – аспирант кафедры челюстно-лицевой и пластической хирургии; сотрудник лаборатории медицинской биорезорбции и биорезистентности 

127473, Москва, ул. Делегатская, 20, стр. 1

Kantemir A. Kunizhev –postgraduate student of the Department of Oral and Maxillofacial and Plastic Surgery; researcher at the Laboratory of Medical Bioresorption and Bioresistance

Delegatskaya St., 20, Bldg. 1, Moscow 127473

kunizhevk@gmail.comhttps://orcid.org/0000-0001-7807-9351РедькоН. А.RedkoN. A.

Редько Николай Андреевич – кандидат медицинских наук, доцент кафедры челюстно-лицевой и пластической хирургии; ведущий научный сотрудник лаборатории медицинской биорезорбции и биорезистентности

127473, Москва, ул. Делегатская, 20, стр. 1

Nikolai A. Redko – Cand Sc. (Med.), associate professor of the Department of Oral and Maxillofacial and Plastic Surgery; leading researcher at the Laboratory of Medical Bioresorption and Bioresistance

Delegatskaya St., 20, Bldg. 1, Moscow 127473

dr.redko@mail.ruhttps://orcid.org/0000-0001-5722-6922ИвановА. А.IvanovA. A.

Иванов Алексей Алексеевич – доктор медицинских наук, заведующий лабораторией клеточной и молекулярной патологии

127473, Москва, ул. Делегатская, 20, стр. 1

Alexey A. Ivanov – Dr. Sc. (Med.), head of the Laboratory of Cellular and Molecular Pathology

Delegatskaya St., 20, Bldg. 1, Moscow 127473

butivanov@yandex.ruНевскаяЕ. Е.NevskayaE. E.

Невская Елена Евгеньевна – сотрудник лаборатории медицинской биорезорбции и биорезистентности

127473, Москва, ул. Делегатская, 20, стр. 1

Elena E. Nevskaya – researcher at the Laboratory of Medical Bioresorption and Bioresistance

Delegatskaya St., 20, Bldg. 1, Moscow 127473

nevskaya_ee@rosunimed.ruhttps://orcid.org/0000-0001-5125-9870КомиссаровА. А.KomissarovA. A.

Комиссаров Александр Александрович – кандидат технических наук, доцент, директор Института «Материаловедение, аддитивные и сквозные технологии», заведующий лабораторией «Гибридные наноструктурные материалы»

119049, Москва, Ленинский пр-кт, д. 4, стр. 1

Alexander A. Komissarov – Cand. Sc. (Engineering), director of the Institute of Materials Science, Additive and End-to-End Technologies; head of the Laboratory of Hybrid Nanostructured Materials

Leninsky Prospekt, 4, Bldg. 1, Moscow 119049

 

Komissarov.alex@gmail.comhttps://orcid.org/0000-0003-3214-1935БаженовВ. Е.BazhenovV. E.

Баженов Вячеслав Евгеньевич – кандидат технических наук, доцент кафедры литейных технологий и художественной обработки материалов; старший научный сотрудник отдела компьютерных технологий и лабораторного обеспечения

119049, Москва, Ленинский пр-кт, д. 4, стр. 1

Vyacheslav E. Bazhenov – Cand. Sc. (Engineering), associate professor of the Department of Foundry Technologies and Artistic Processing of Materials; senior researcher

Leninsky Prospekt, 4, Bldg. 1, Moscow 119049

v.e.bagenov@gmail.comhttps://orcid.org/0000-0002-1710-6923ДробышевА. Ю.DrobyshevA. Yu.

Дробышев Алексей Юрьевич – доктор медицинских наук, член-корреспондент РАН, профессор, заведующий кафедрой челюстно-лицевой и пластической хирургии 

127473, Москва, ул. Делегатская, 20, стр. 1

Alexey Yu. Drobyshev – Dr. Sc. (Med.), corresponding member of the RAS, professor, head of the Department of Oral and Maxillofacial and Plastic Surgery

Delegatskaya St., 20, Bldg. 1, Moscow 127473

dr.drobyshev@gmail.comФГБОУ ВО «Российский университет медицины» Минздрава РоссииРоссияRussian University of Medicine, Ministry of Health of the Russian FederationRussian FederationФГАОУ ВО «Национальный исследовательский технологический университет «МИСИС»РоссияNational University of Science and Technology “MISIS”Russian Federation202617102026114215224Copyright © Кунижев К.А., Редько Н.А., Иванов А.А., Невская Е.Е., Комиссаров А.А., Баженов В.Е., Дробышев А.Ю., 20262026Кунижев К.А., Редько Н.А., Иванов А.А., Невская Е.Е., Комиссаров А.А., Баженов В.Е., Дробышев А.Ю.Kunizhev K.A., Redko N.A., Ivanov A.A., Nevskaya E.E., Komissarov A.A., Bazhenov V.E., Drobyshev A.Y.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.actabiomedica.ru/jour/article/view/6337Обоснование

Обоснование. Клинический потенциал магниевых имплантатов определяется характером деградации и реакцией окружающих тканей. Для Mg–2Zn–2Ga недостаточно изучено соответствие лучевых и морфологических изменений в парной подкожной модели с титановым контролем.

Цель

Цель. Сопоставить МСКТ-признаки деградации Mg–2Zn–2Ga и газообразования с морфологической реакцией мягких тканей в сравнении с титаном в сроки 1–11 недель.

Методы

Методы. Исследование выполнено на 20 самцах крыс линии Wistar. Каждому животному подкожно над лопатками устанавливались диски Mg–2Zn–2Ga слева и титана справа диаметром 7 мм и толщиной 4 мм. На сроках 1, 2, 3, 4 и 11 недель (по 4 животных) проводились МСКТ, макроскопическая оценка и гистологическое исследование обеих зон имплантации.

Результаты

Результаты. Через 1 неделю вокруг Mg–2Zn–2Ga выявлялась газовая полость и отмечалось небольшое снижение рентгеноплотности. На сроках 2–4 недели лучевые изменения становились заметнее; к 4-й неделе магниевый образец визуально был меньше стабильного титанового имплантата. На 11-й неделе сохранялись остаточная газовая полость, снижение рентгеноплотности и размеров Mg–2Zn–2Ga. Морфологически ранняя полость с тонкой фиброзной капсулой и лимфогистиоцитарной инфильтрацией сменялась клеточным валом и множественными полостями с фрагментацией прилежащих мышечных волокон; к 11-й неделе выявлялись единичные гигантские клетки инородных тел. Вокруг титана преобладала последовательная соединительнотканная организация. Крепитация над магниевым имплантатом отмечалась у 3 из 20 животных (15 %).

Заключение

Заключение. Сопоставление МСКТ и морфологии выявило согласованную временную последовательность признаков деградации Mg–2Zn–2Ga и перестройки окружающих тканей, отличавшуюся от реакции на титан. Сроки 2–4 недели характеризовали развитие ранних изменений, а 11 недель — их сохранение. Эти сроки и признаки могут использоваться в последующей количественной доклинической оценке.

Background

Background. The clinical potential of magnesium implants depends on their degradation behavior and the response of surrounding tissues. For Mg–2Zn–2Ga, the correspondence between radiological and morphological changes in a paired subcutaneous model with a titanium control has not been adequately studied.

Aim

Aim. To compare MSCT signs of Mg–2Zn–2Ga degradation and gas formation with the morphological response of soft tissues relative to titanium at 1–11 weeks.

Materials and methods

Materials and methods. The study was performed on 20 male Wistar rats. Discs measuring 7 mm in diameter and 4 mm in thickness were implanted subcutaneously over the scapulae of each animal: Mg–2Zn–2Ga on the left and titanium on the right. At weeks 1, 2, 3, 4, and 11 (four animals per time point), MSCT, macroscopic assessment, and histological examination of both implantation sites were performed.

Results

Results. At week 1, a gas cavity was detected around Mg–2Zn–2Ga, and a slight reduction in radiodensity was observed. At weeks 2–4, radiological changes became more apparent; by week 4, the magnesium specimen was visually smaller than the stable titanium implant. At week 11, a residual gas cavity and reduced radiodensity and dimensions of Mg–2Zn–2Ga persisted. Histologically, the early cavity with a thin fibrous capsule and lymphohistiocytic infiltration was followed by a cellular rim and multiple cavities with fragmentation of adjacent muscle fibers; occasional foreign-body giant cells were detected by week 11. Orderly connective-tissue organization predominated around titanium. Crepitus over the magnesium implant was observed in 3 of 20 animals (15 %).

Conclusion

Conclusion. Comparison of MSCT and histology revealed a concordant time course of Mg–2Zn–2Ga degradation signs and surrounding tissue remodeling that differed from the response to titanium. Early changes developed at weeks 2–4 and persisted at week 11. These time points and features may be used in subsequent quantitative preclinical assessment.

магниевые сплавыMg–2Zn–2Gaкоррозионная деградациямультиспиральная компьютерная томографиягазовая полостьмягкие тканигистологическое исследованиетитанmagnesium alloysMg–2Zn–2Gacorrosion degradationmultislice computed tomographygas cavitysoft tissueshistologytitaniumИсследование было поддержано Министерством здравоохранения Российской Федерации в рамках Государственного задания № 05600052-26-02ReferencesStaiger MP, Pietak AM, Huadmai J, Dias G. Magnesium and its alloys as orthopedic biomaterials: a review. Biomaterials. 2006; 27(9): 1728–1734. doi: 10.1016/j.biomaterials.2005.10.003Staiger MP, Pietak AM, Huadmai J, Dias G. Magnesium and its alloys as orthopedic biomaterials: a review. Biomaterials. 2006; 27(9): 1728–1734. doi: 10.1016/j.biomaterials.2005.10.003Zhao D, Witte F, Lu F, Wang J, Li J, Qin L. Current status on clinical applications of magnesium-based orthopaedic implants: a review from clinical translational perspective. Biomaterials. 2017; 112: 287–302. doi: 10.1016/j.biomaterials.2016.10.017Zhao D, Witte F, Lu F, Wang J, Li J, Qin L. Current status on clinical applications of magnesium-based orthopaedic implants: a review from clinical translational perspective. Biomaterials. 2017; 112: 287–302. doi: 10.1016/j.biomaterials.2016.10.017Herber V, Okutan B, Antonoglou G, Sommer NG, Payer M. Bioresorbable magnesium-based alloys as novel biomaterials in oral bone regeneration: general review and clinical perspectives. J Clin Med. 2021; 10 (9): 1842. doi: 10.3390/jcm10091842Herber V, Okutan B, Antonoglou G, Sommer NG, Payer M. Bioresorbable magnesium-based alloys as novel biomaterials in oral bone regeneration: general review and clinical perspectives. J Clin Med. 2021; 10 (9): 1842. doi: 10.3390/jcm10091842Gareb B, van Bakelen NB, Vissink A, Bos RRM, van Minnen B. Titanium or biodegradable osteosynthesis in maxillofacial surgery? In vitro and in vivo performances. Polymers (Basel). 2022; 14 (14): 2782. doi: 10.3390/polym14142782Gareb B, van Bakelen NB, Vissink A, Bos RRM, van Minnen B. Titanium or biodegradable osteosynthesis in maxillofacial surgery? In vitro and in vivo performances. Polymers (Basel). 2022; 14 (14): 2782. doi: 10.3390/polym14142782Vujović S, Desnica J, Stanišić D, Ognjanović I, Stevanovic M, Rosic G. Applications of biodegradable magnesium-based materials in reconstructive oral and maxillofacial surgery: a review. Molecules. 2022; 27(17): 5529. doi: 10.3390/molecules27175529Vujović S, Desnica J, Stanišić D, Ognjanović I, Stevanovic M, Rosic G. Applications of biodegradable magnesium-based materials in reconstructive oral and maxillofacial surgery: a review. Molecules. 2022; 27(17): 5529. doi: 10.3390/molecules27175529Wang JL, Xu JK, Hopkins C, Chow DHK, Qin L. Biodegradable magnesium-based implants in orthopedics — A general review and perspectives. Adv Sci (Weinh). 2020; 7(8): 1902443. doi: 10.1002/advs.201902443Wang JL, Xu JK, Hopkins C, Chow DHK, Qin L. Biodegradable magnesium-based implants in orthopedics — A general review and perspectives. Adv Sci (Weinh). 2020; 7(8): 1902443. doi: 10.1002/advs.201902443Nasr Azadani M, Zahedi A, Bowoto OK, Oladapo BI. A review of current challenges and prospects of magnesium and its alloy for bone implant applications. Prog Biomater. 2022; 11(1): 1–26. doi: 10.1007/s40204-022-00182-xNasr Azadani M, Zahedi A, Bowoto OK, Oladapo BI. A review of current challenges and prospects of magnesium and its alloy for bone implant applications. Prog Biomater. 2022; 11(1): 1–26. doi: 10.1007/s40204-022-00182-xWitte F, Kaese V, Haferkamp H, Switzer E, Meyer-Lindenberg A, Wirth CJ, et al. In vivo corrosion of four magnesium alloys and the associated bone response. Biomaterials. 2005; 26(17): 3557–3563. doi: 10.1016/j.biomaterials.2004.09.049Witte F, Kaese V, Haferkamp H, Switzer E, Meyer-Lindenberg A, Wirth CJ, et al. In vivo corrosion of four magnesium alloys and the associated bone response. Biomaterials. 2005; 26(17): 3557–3563. doi: 10.1016/j.biomaterials.2004.09.049Amerstorfer F, Fischerauer SF, Fischer L, Eichler J, Draxler J, Zitek A, et al. Long-term in vivo degradation behavior and near-implant distribution of resorbed elements for magnesium alloys WZ21 and ZX50. Acta Biomater. 2016; 42: 440–450. doi: 10.1016/j.actbio.2016.06.025Amerstorfer F, Fischerauer SF, Fischer L, Eichler J, Draxler J, Zitek A, et al. Long-term in vivo degradation behavior and near-implant distribution of resorbed elements for magnesium alloys WZ21 and ZX50. Acta Biomater. 2016; 42: 440–450. doi: 10.1016/j.actbio.2016.06.025Byun SH, Lim HK, Cheon KH, Lee SM, Kim HE, Lee JH. Biodegradable magnesium alloy (WE43) in bone-fixation plate and screw. J Biomed Mater Res B Appl Biomater. 2020; 108(6): 2505–2512. doi: 10.1002/jbm.b.34582Byun SH, Lim HK, Cheon KH, Lee SM, Kim HE, Lee JH. Biodegradable magnesium alloy (WE43) in bone-fixation plate and screw. J Biomed Mater Res B Appl Biomater. 2020; 108(6): 2505–2512. doi: 10.1002/jbm.b.34582Guo Y, Yu Y, Han L, Ma S, Zhao J, Chen H, et al. Biocompatibility and osteogenic activity of guided bone regeneration membrane based on chitosan-coated magnesium alloy. Mater Sci Eng C Mater Biol Appl. 2019; 100: 226–235. doi: 10.1016/j.msec.2019.03.006Guo Y, Yu Y, Han L, Ma S, Zhao J, Chen H, et al. Biocompatibility and osteogenic activity of guided bone regeneration membrane based on chitosan-coated magnesium alloy. Mater Sci Eng C Mater Biol Appl. 2019; 100: 226–235. doi: 10.1016/j.msec.2019.03.006Sun Y, Helmholz H, Willumeit-Römer R. Peri-implant gas accumulation in response to magnesium-based musculoskeletal biomaterials: reframing current evidence for preclinical research and clinical evaluation. Journal of Magnesium and Alloys. 2024; 12(1): 59–71. doi: 10.1016/j.jma.2024.01.023Sun Y, Helmholz H, Willumeit-Römer R. Peri-implant gas accumulation in response to magnesium-based musculoskeletal biomaterials: reframing current evidence for preclinical research and clinical evaluation. Journal of Magnesium and Alloys. 2024; 12(1): 59–71. doi: 10.1016/j.jma.2024.01.023Ben Amara H, Martinez DC, Shah FA, Loo AJ, Emanuelsson L, Norlindh B, et al. Magnesium implant degradation provides immunomodulatory and proangiogenic effects and attenuates peri-implant fibrosis in soft tissues. Bioact Mater. 2023; 26: 353–369. doi: 10.1016/j.bioactmat.2023.02.014Ben Amara H, Martinez DC, Shah FA, Loo AJ, Emanuelsson L, Norlindh B, et al. Magnesium implant degradation provides immunomodulatory and proangiogenic effects and attenuates peri-implant fibrosis in soft tissues. Bioact Mater. 2023; 26: 353–369. doi: 10.1016/j.bioactmat.2023.02.014Bazhenov VE, Koltygin AV, Komissarov AA, Li A, Bautin V, Khasenova R, et al. Gallium-containing magnesium alloy for potential use as temporary implants in osteosynthesis. Journal of Magnesium and Alloys. 2020; 8(2): 352–363. doi: 10.1016/j.jma.2020.02.009Bazhenov VE, Koltygin AV, Komissarov AA, Li A, Bautin V, Khasenova R, et al. Gallium-containing magnesium alloy for potential use as temporary implants in osteosynthesis. Journal of Magnesium and Alloys. 2020; 8(2): 352–363. doi: 10.1016/j.jma.2020.02.009Bazhenov VE, Li A, Iliasov A, Bautin V, Plegunova S, Koltygin A, et al. Corrosion behavior and biocompatibility of hot-extruded Mg–Zn–Ga–(Y) biodegradable alloys. J Funct Biomater. 2022; 13(4): 294. doi: 10.3390/jfb13040294Bazhenov VE, Li A, Iliasov A, Bautin V, Plegunova S, Koltygin A, et al. Corrosion behavior and biocompatibility of hot-extruded Mg–Zn–Ga–(Y) biodegradable alloys. J Funct Biomater. 2022; 13(4): 294. doi: 10.3390/jfb13040294Rogachev SO, Bazhenov VE, Komissarov AA, Li AV, Munzaferova NE, Plegunova SV, et al. Microstructure, mechanical, and corrosion properties of Mg–Zn–Ga alloy after hot rolling. Journal of Materials Engineering and Performance. 2025; 34(5): 3970–3978. doi: 10.1007/s11665-024-09361-2Rogachev SO, Bazhenov VE, Komissarov AA, Li AV, Munzaferova NE, Plegunova SV, et al. Microstructure, mechanical, and corrosion properties of Mg–Zn–Ga alloy after hot rolling. Journal of Materials Engineering and Performance. 2025; 34(5): 3970–3978. doi: 10.1007/s11665-024-09361-2Drobyshev A, Gurganchova Z, Redko N, Komissarov A, Bazhenov V, Statnik ES, et al. An in vivo rat study of bioresorbable Mg–2Zn–2Ga alloy implants. Bioengineering (Basel). 2023; 10(2): 273. doi: 10.3390/bioengineering10020273Drobyshev A, Gurganchova Z, Redko N, Komissarov A, Bazhenov V, Statnik ES, et al. An in vivo rat study of bioresorbable Mg–2Zn–2Ga alloy implants. Bioengineering (Basel). 2023; 10(2): 273. doi: 10.3390/bioengineering10020273Drobyshev A, Komissarov A, Redko N, Gurganchova Z, Statnik ES, Bazhenov V, et al. Bone remodeling interaction with magnesium alloy implants studied by SEM and EDX. Materials (Basel). 2022; 15(21): 7529. doi: 10.3390/ma15217529Drobyshev A, Komissarov A, Redko N, Gurganchova Z, Statnik ES, Bazhenov V, et al. Bone remodeling interaction with magnesium alloy implants studied by SEM and EDX. Materials (Basel). 2022; 15(21): 7529. doi: 10.3390/ma15217529

The authors declare that there are no conflicts of interest present.