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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">actabiomedica</journal-id><journal-title-group><journal-title xml:lang="ru">Acta Biomedica Scientifica</journal-title><trans-title-group xml:lang="en"><trans-title>Acta Biomedica Scientifica</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">2541-9420</issn><issn pub-type="epub">2587-9596</issn><publisher><publisher-name>Scientific Centre for Family Health and Human Reproduction Problems</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.29413/ABS.2025-10.3.8</article-id><article-id custom-type="elpub" pub-id-type="custom">actabiomedica-5342</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>МИКРОБИОЛОГИЯ И ВИРУСОЛОГИЯ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>MICROBIOLOGY AND VIRUSOLOGY</subject></subj-group></article-categories><title-group><article-title>Новые данные об инфицированности Haemaphysalis concinna возбудителями клещевых инфекций человека и животных на территории Прибайкалья</article-title><trans-title-group xml:lang="en"><trans-title>New data on infection of Haemaphysalis concinna with tick-borne infection agents in humans and animals in the territory of Baikal region</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0003-4057-2890</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Сунцова</surname><given-names>О. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Suntsova</surname><given-names>O. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Сунцова Ольга Владимировна – кандидат биологических наук, научный сотрудник лаборатории молекулярной эпидемиологии и генетической диагностики </p><p>664003, г. Иркутск, ул. Тимирязева, 16</p></bio><bio xml:lang="en"><p>Olga V. Suntsova – Cand. Sc. (Biol.), Research Officer at the Laboratory of molecular epidemiology and genetic diagnostics </p><p>Timiryazeva str., 16, Irkutsk 664003</p></bio><email xlink:type="simple">olga_syntsova@list.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-5930-5306</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Рар</surname><given-names>В. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Rar</surname><given-names>V. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Рар Вера Александровна – доктор биологических наук, ведущий научный сотрудник лаборатории молекулярной микробиологии </p><p>630090, г. Новосибирск, просп. Академика Лаврентьева, 8</p></bio><bio xml:lang="en"><p>Vera A. Rar – Dr. Sc. (Biol.), Leading Research Officer </p><p>Lavrentyeva av., 8, Novosibirsk 630090</p></bio><email xlink:type="simple">rarv@niboch.nsc.ru</email><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0003-3909-7551</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Лисак</surname><given-names>О. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Lisak</surname><given-names>O. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Лисак Оксана Васильевна – младший научный сотрудник лаборатории молекулярной эпидемиологии и генетической диагностики </p><p>664003, г. Иркутск, ул. Тимирязева, 16</p></bio><bio xml:lang="en"><p>Oksana V. Lisak – Junior Research Officer at the Laboratory of molecular epidemiology and genetic diagnostics </p><p>Timiryazeva str., 16, Irkutsk 664003</p></bio><email xlink:type="simple">lisak.liza@rambler.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-8209-616X</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Дорощенко</surname><given-names>Е. К.</given-names></name><name name-style="western" xml:lang="en"><surname>Doroshchenko</surname><given-names>E. K.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Дорощенко Елена Константиновна – кандидат биологических наук, научный сотрудник лаборатории молекулярной эпидемиологии и генетической диагностики </p><p>664003, г. Иркутск, ул. Тимирязева, 16</p></bio><bio xml:lang="en"><p>Elena K. Doroshchenko – Cand. Sc. (Biol.), Research Officer at the Laboratory of molecular epidemiology and genetic diagnostics </p><p>Timiryazeva str., 16, Irkutsk 664003</p></bio><email xlink:type="simple">doroshchenko-virus@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0003-2222-4518</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Арефьева</surname><given-names>Н. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Arefieva</surname><given-names>N. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Арефьева Надежда Александровна – младший научный сотрудник лаборатории молекулярной эпидемиологии и генетической диагностики </p><p>664003, г. Иркутск, ул. Тимирязева, 16</p></bio><bio xml:lang="en"><p>Nadezhda A. Arefieva – Junior Research Officer at the Laboratory of molecular epidemiology and genetic diagnostics </p><p>Timiryazeva str., 16, Irkutsk 664003</p></bio><email xlink:type="simple">arefieva.n4@gmail.com</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-6324-8746</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Козлова</surname><given-names>И. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Kozlova</surname><given-names>I. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Козлова Ирина Валерьевна – доктор медицинских наук, руководитель лаборатории молекулярной эпидемиологии и генетической диагностики </p><p>664003, г. Иркутск, ул. Тимирязева, 16</p></bio><bio xml:lang="en"><p>Irina V. Kozlova – Dr. Sc. (Med.), Head of the Laboratory of molecular epidemiology and genetic diagnostics </p><p>Timiryazeva str., 16, Irkutsk 664003</p></bio><email xlink:type="simple">diwerhoz@rambler.ru</email><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>ФГБНУ «Научный центр проблем здоровья семьи и репродукции человека»</institution></aff><aff xml:lang="en"><institution>Scientific Center for Family Health for Human Reproduction problems</institution></aff></aff-alternatives><aff-alternatives id="aff-2"><aff xml:lang="ru"><institution>ФГБУН Институт химической биологии и фундаментальной медицины СО РАН</institution></aff><aff xml:lang="en"><institution>Institute of Chemical Biology and Fundamental Medicine, SB of the RAS</institution></aff></aff-alternatives><pub-date pub-type="collection"><year>2025</year></pub-date><pub-date pub-type="epub"><day>22</day><month>07</month><year>2025</year></pub-date><volume>10</volume><issue>3</issue><fpage>80</fpage><lpage>90</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Сунцова О.В., Рар В.А., Лисак О.В., Дорощенко Е.К., Арефьева Н.А., Козлова И.В., 2025</copyright-statement><copyright-year>2025</copyright-year><copyright-holder xml:lang="ru">Сунцова О.В., Рар В.А., Лисак О.В., Дорощенко Е.К., Арефьева Н.А., Козлова И.В.</copyright-holder><copyright-holder xml:lang="en">Suntsova O.V., Rar V.A., Lisak O.V., Doroshchenko E.K., Arefieva N.A., Kozlova I.V.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://www.actabiomedica.ru/jour/article/view/5342">https://www.actabiomedica.ru/jour/article/view/5342</self-uri><abstract><p>Обоснование. Haemaphysalis concinna привлекает к себе большое внимание в связи с расширением ареала и возрастающей ролью в передаче человеку и животным заболеваний различной этиологии. На территории Прибайкалья изучение инфицированности H. concinna почти не проводилось, несмотря на способность этих клещей переносить возбудителей, патогенных для человека. Цель исследования. Изучение инфицированности Haemaphysalis concinna возбудителями клещевых инфекций человека и животных на территории Прибайкалья. Материалы и методы. Из природных стаций Прибайкалья было исследовано ПЦР методом 998 экземпляров клещей H. concinna на наличие маркеров различных патогенов вирусной, бактериальной и протозойной природы. Результаты. В клещах H. concinna нами выявлены маркеры следующих патогенов: РНК ВКЭ (0,6 %), вируса Кемерово (1,1 %), ДНК Borrelia burgdorferi s.l. (1,3 %), B. miyamotoi (0,5 %), ДНК представителей семейства Anaplasmataceae (E. muris и A. phagocytophilum) (3,7 %), риккетсий (R. sibirica (1,6 %), R. raoultii (2,8 %)), «Candidatus R. tarasevichiae» (32,6 %) а также простейших (Babesia spp.) (3,5 %). Определенная в ходе исследования нуклеотидная последовательность groESL оперона длиной 1315 н.о. образца ДНК Ehrlichia spp. от клеща H. concinna оказалась идентичной нуклеотидным последовательностям образцов E. muris. На основании анализа последовательностей гена 18S рРНК в образцах клещей H. concinna, нами выявлены бабезии генетически близкие к пироплазмидам мелких жвачных – B. crassa и B. motasi. Впервые обнаружен образец ДНК Babesia sp., нуклеотидные последовательности которого идентичны последовательностям образца Kh-Hc222 (KJ486568), обнаруженного в Хабаровском крае. Заключение. Полученные нами данные свидетельствуют о необходимости организации постоянного мониторинга за местообитаниями клещей H. concinna на территории Прибайкалья и изучении его инфицированности как известными, так и новыми, недавно выявленными трансмиссивными клещевыми патогенами.</p></abstract><trans-abstract xml:lang="en"><p>Background. In recent years, the tick Haemaphysalis concinna has drawn increased attention from researchers due to its expanding geographic range and its growing role in the transmission of diseases of various etiologies to humans and animals. However, studies on the infection of H. concinna with tick-borne pathogens affecting humans and animals in the Baikal region have been minimal, despite its known capacity to carry at least 40 pathogen species pathogenic to humans. The aim. To investigate the infection of Haemaphysalis concinna with tick-borne pathogens affecting humans and animals in the Baikal region. Materials and Methods. A total of 998 specimens of H. concinna ticks collected from natural habitats in the Baikal region were analyzed by PCR for the presence of markers of various viral, bacterial, and protozoan pathogens. Results. In the examined H. concinna ticks, the following pathogens were detected: Kemerovo virus RNA (1.1 %), Borrelia burgdorferi sensu lato (1.3 %), Borrelia miyamotoi (0.5 %), Ehrlichia sp. / Anaplasma sp. (3.7 %), Rickettsia sibirica (1.6 %), Rickettsia raoultii (2.8 %), «Candidatus R. tarasevichiae» (32.6 %) and Babesia spр. (3.5 %). Tickborne encephalitis virus (TBEV) RNA was not detected in this study; however, in earlier PCR-based investigations, TBEV RNA was found in 0.6 ± 0.6 % of H. concinna samples from the Baikal region. In one tick sample, the nucleotide sequence of the groESL operon (1315 bp) was identified and found to be identical to sequences of Ehrlichia muris. Based on 18S rRNA gene sequence analysis, Babesia species genetically close to small ruminant piroplasms – Babesia crassa and Babesia motasi – were identified. For the first time, a Babesia sp. DNA sample was detected with nucleotide sequences identical to the Kh-Hc222 strain (KJ486568), previously identified in Khabarovsk Krai. Conclusion. The data obtained underscore the need for continuous monitoring of H. concinna tick populations in the Baikal region and further investigation into their infection with both well-known and newly identified vector-borne pathogens.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>Haemaphysalis concinna</kwd><kwd>вирус Кемерово</kwd><kwd>боррелии</kwd><kwd>риккетсии</kwd><kwd>анаплазмы</kwd><kwd>эрлихии</kwd><kwd>бабезии</kwd></kwd-group><kwd-group xml:lang="en"><kwd>Haemaphysalis concinna</kwd><kwd>Kemerovo virus</kwd><kwd>Borrelia</kwd><kwd>Rickettsia</kwd><kwd>Anaplasma</kwd><kwd>Ehrlichia</kwd><kwd>Babesia</kwd></kwd-group><funding-group><funding-statement xml:lang="ru">Исследование поддержано в рамках государственного задания ФГБНУ НЦ ПЗСРЧ №  1021060107125-0 и ИХБФМ СО РАН № 125012300671-8.</funding-statement></funding-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Rubel F, Brugger K, Walter M, Vogelgesang JR, Didyk YM, Fu S, et al. 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