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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">actabiomedica</journal-id><journal-title-group><journal-title xml:lang="ru">Acta Biomedica Scientifica</journal-title><trans-title-group xml:lang="en"><trans-title>Acta Biomedica Scientifica</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">2541-9420</issn><issn pub-type="epub">2587-9596</issn><publisher><publisher-name>Scientific Centre for Family Health and Human Reproduction Problems</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.29413/ABS.2024-9.6.25</article-id><article-id custom-type="elpub" pub-id-type="custom">actabiomedica-5143</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>ЭПИДЕМИОЛОГИЯ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>EPIDEMIOLOGY</subject></subj-group></article-categories><title-group><article-title>Иксодовые клещи как  переносчики возбудителей бабезиозов человека и  животных в  Прибайкалье</article-title><trans-title-group xml:lang="en"><trans-title>Ixodes ticks as carriers of human and animal babesiosis agents in  the  Baikal region</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0003-4057-2890</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Сунцова</surname><given-names>О. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Suntsova</surname><given-names>O. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Сунцова Ольга Владимировна – кандидат биологических наук, научный сотрудник лаборатории молекулярной эпидемиологии и генетической диагностики,</p><p>664003, г. Иркутск, ул. Тимирязева, 16</p></bio><bio xml:lang="en"><p>Olga V. Suntsova – Cand. Sc. (Biol.), Research Officer at the Laboratory of Molecular Epidemiology and Genetic Diagnostics, </p><p>Timiryazeva str. 16, Irkutsk 664003</p></bio><email xlink:type="simple">olga_syntsova@list.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-5930-5306</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Рар</surname><given-names>В. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Rar</surname><given-names>V. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Рар Вера Александровна – доктор биологических наук, ведущий научный сотрудник лаборатории молекулярной микробиологии, </p><p>630090, г. Новосибирск, просп. Академика Лаврентьева, 8</p></bio><bio xml:lang="en"><p>Vera A. Rar – Dr. Sc. (Biol.), Leading Research Officer at the Laboratory of Molecular Microbiology, </p><p>Molodezhny settlement 1/1, Irkutsk district 664038</p></bio><email xlink:type="simple">rarv@niboch.nsc.ru</email><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-8566-7004</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Мельцов</surname><given-names>И. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Meltsov</surname><given-names>I. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Мельцов Иван Владимирович – кандидат ветеринарных наук, доцент кафедры специальных ветеринарных дисциплин, </p><p>664038, Иркутский район, пос. Молодёжный, 1/1</p></bio><bio xml:lang="en"><p>Ivan V. Meltsov – Cand. Sc. (Vet.), Associate Professor at the Department of Special Veterinary Disciplines, </p><p>Molodezhny settlement 1/1, Irkutsk district 664038</p></bio><email xlink:type="simple">ivanmeltsov@mail.ru</email><xref ref-type="aff" rid="aff-3"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0003-3909-7551</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Лисак</surname><given-names>О. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Lisak</surname><given-names>O. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Лисак Оксана Васильевна – младший научный сотрудник лаборатории молекулярной эпидемиологии и генетической диагностики, </p><p>664003, г. Иркутск, ул. Тимирязева, 16</p></bio><bio xml:lang="en"><p>Oksana  V. Lisak – Junior Research Officer at  the  Laboratory of  Molecular Epidemiology and  Genetic Diagnostics, </p><p>Timiryazeva str. 16, Irkutsk 664003</p></bio><email xlink:type="simple">lisak.liza@rambler.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-8209-616X</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Дорощенко</surname><given-names>Е. К.</given-names></name><name name-style="western" xml:lang="en"><surname>Doroshchenko</surname><given-names>E. K.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Дорощенко Елена Константиновна – кандидат биологических наук, научный сотрудник лаборатории молекулярной эпидемиологии и генетической диагностики, </p><p>664003, г. Иркутск, ул. Тимирязева, 16</p></bio><bio xml:lang="en"><p>Elena K. Doroshchenko – Cand. Sc. (Biol.), Research Officer at the Laboratory of Molecular Epidemiology and Genetic Diagnostics,</p><p>Timiryazeva str. 16, Irkutsk 664003</p></bio><email xlink:type="simple">doroshchenko-virus@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-6324-8746</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Козлова</surname><given-names>И. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Kozlova</surname><given-names>I. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Козлова Ирина Валерьевна – доктор медицинских наук, руководитель лаборатории молекулярной эпидемиологии и генетической диагностики, </p><p>664003, г. Иркутск, ул. Тимирязева, 16</p></bio><bio xml:lang="en"><p>Irina  V. Kozlova – Dr.  Sc. (Med.), Head of  the  Laboratory of  Molecular Epidemiology and  Genetic Diagnostics, </p><p>Timiryazeva str. 16, Irkutsk 664003</p></bio><email xlink:type="simple">diwerhoz@rambler.ru</email><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>ФГБНУ «Научный центр проблем здоровья семьи и репродукции человека»</institution></aff><aff xml:lang="en"><institution>Scientific Centre for Family Health and Human Reproduction Problems</institution></aff></aff-alternatives><aff-alternatives id="aff-2"><aff xml:lang="ru"><institution>ФГБУН Институт химической биологии и фундаментальной медицины СО РАН</institution></aff><aff xml:lang="en"><institution>Institute of Chemical Biology and Fundamental Medicine SB RAS</institution></aff></aff-alternatives><aff-alternatives id="aff-3"><aff xml:lang="ru"><institution>ФГБОУ ВО «Иркутский государственный аграрный университет им. А.А. Ежевского»</institution></aff><aff xml:lang="en"><institution>Irkutsk State Agrarian University named after A.A. Ezhevsky</institution></aff></aff-alternatives><pub-date pub-type="collection"><year>2024</year></pub-date><pub-date pub-type="epub"><day>28</day><month>12</month><year>2024</year></pub-date><volume>9</volume><issue>6</issue><fpage>249</fpage><lpage>257</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Сунцова О.В., Рар В.А., Мельцов И.В., Лисак О.В., Дорощенко Е.К., Козлова И.В., 2024</copyright-statement><copyright-year>2024</copyright-year><copyright-holder xml:lang="ru">Сунцова О.В., Рар В.А., Мельцов И.В., Лисак О.В., Дорощенко Е.К., Козлова И.В.</copyright-holder><copyright-holder xml:lang="en">Suntsova O.V., Rar V.A., Meltsov I.V., Lisak O.V., Doroshchenko E.K., Kozlova I.V.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://www.actabiomedica.ru/jour/article/view/5143">https://www.actabiomedica.ru/jour/article/view/5143</self-uri><abstract><sec><title>Обоснование</title><p>Обоснование. Пироплазмозы (бабезиозы и  тейлериозы) – это  группа природно-очаговых инфекционных заболеваний человека и  животных, вызываемых внутриэритроцитарными паразитами, которые передаются преимущественно иксодовыми клещами. В  Прибайкалье, несмотря на широкое распространение очагов бабезиоза и тейлериоза, информация о переносчиках этих заболеваний фрагментарна.</p></sec><sec><title>Цель исследования</title><p>Цель исследования. Выявление переносчиков возбудителей бабезиоза человека и животных, изучение тропизма различных видов бабезий к иксодовым клещам, распространённым в Прибайкалье.</p></sec><sec><title>Материалы и методы</title><p>Материалы и методы. На наличие ДНК Babesia spp./Theileria spp. исследовано 3239 экземпляров I. persulcatus, 1795 экземпляров D. nuttalli и 729 экземпляров H. сoncinna, собранных в природных стациях Прибайкалья из 19 районов Иркутской области и 6 районов Республики Бурятия, а также 5 экземпляров D. nuttalli, снятых с лошадей. Для детекции и типирования бабезий и тейлерий использована гнездовая двухраундовая полимеразная цепная реакция с родо- и видоспецифичными праймерами из области гена 18S рРНК. Для изучения генетического разнообразия выявленных возбудителей проводилось выборочное секвенирование образцов.</p></sec><sec><title>Результаты</title><p>Результаты. ДНК Babesia  spp. выявлена в  трёх видах иксодовых клещей на  территории Иркутской области и  в  одном – в  Республике Бурятия. В клещах I. persulcatus обнаружены: B. microti «US»-type, B. venatorum, B. crassalike и  уникальная Babesia  sp. Irk-Ip655. В  клещах H.  concinna выявлена ДНК B. crassa-like, B. motasi-like и Babesia spp. В клещах D. nuttalli обнаружена ДНК Babesia spp. и T. equi.</p></sec><sec><title>Заключение</title><p>Заключение. На территории Прибайкалья выявлены переносчики возбудителей бабезиоза как человека, так и животных. Установлен тропизм B. microti «US»-type и B. venatorum в отношении клещей I. persulcatus. Выявление специфических связей между другими видами бабезий и тейлерий и клещамипереносчиками на территории Прибайкалья будет продолжено.</p></sec></abstract><trans-abstract xml:lang="en"><sec><title>Background</title><p>Background. Piroplasmosis (babesiosis and theileriosis) is a group of natural focal infectious diseases of  humans and  animals caused by  intraerythrocytic parasites transmitted predominantly by ixodid ticks. In the Baikal region, despite the widespread foci of babesiosis and theileriosis, the data about these pathogen vectors is fragmentary.</p></sec><sec><title>The aim</title><p>The aim. To identify human and animal babesiosis vectors, study various Babesia spp. tropism to ixodid ticks common in the Baikal region.</p></sec><sec><title>Materials and methods</title><p>Materials and methods. A total of 3,239 specimens of I. persulcatus, 1,795 specimens of D. nuttalli and 729 specimens of H. сoncinna ticks collected in natural habitats of the Baikal region from 19 districts of the Irkutsk region and six districts of the Republic of Buryatia, as well as 5 specimens of D. nuttalli ticks removed from horses were examined for the presence of Babesia spp./Theileria spp. DNA. For detection and typing of Babesia and Theileria, a nested PCR with genus- and species-specific primers corresponding to  18S  rRNA gene region was used. Selective sequencing of samples was performed to study the genetic diversity of the identified pathogens.</p></sec><sec><title>Results</title><p>Results. Babesia spp. DNA was detected in three species of ixodid ticks in Irkutsk region and in one species in the Republic of Buryatia. In I. persulcatus ticks B. microti US-type, B. venatorum, B. crassa-like and a unique Babesia sp. Irk-Ip655 were found. DNAs of B. crassa-like, B. motasi-like and Babesia spp. were detected in H. concinna ticks. DNAs of Babesia spp. And T. equi were found in D. nuttalli ticks.</p></sec><sec><title>Conclusion</title><p>Conclusion. Vectors of  both human and  animal babesiosis pathogens were  detected in the Baikal region. Tropism of B. microti US-type and B. venatorum in relation to I. persulcatus ticks was established. The establishment of specific relations between other species of Babesia and Theileria and vector ticks in the Baikal region will be continued. </p></sec></trans-abstract><kwd-group xml:lang="ru"><kwd>бабезии</kwd><kwd>тейлерии</kwd><kwd>иксодовые клещи</kwd><kwd>тропизм</kwd><kwd>видовое разнообразие</kwd></kwd-group><kwd-group xml:lang="en"><kwd>Babesia</kwd><kwd>Theileria</kwd><kwd>ixodid ticks</kwd><kwd>tropism</kwd><kwd>species diversity</kwd></kwd-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Vannier EG, Diuk-Wasser MA, Ben Mamoun C, Krause PJ. Babesiosis. Infect Dis Clin North Am. 2015; 29: 357-370. doi: 10.1016/j.idc.2015.02.008</mixed-citation><mixed-citation xml:lang="en">Vannier EG, Diuk-Wasser MA, Ben Mamoun C, Krause PJ. Babesiosis. Infect Dis Clin North Am. 2015; 29: 357-370. doi: 10.1016/j.idc.2015.02.008</mixed-citation></citation-alternatives></ref><ref id="cit2"><label>2</label><citation-alternatives><mixed-citation xml:lang="ru">Onyiche TE, Răileanu C, Fischer S, Silaghi C. Global distribution of Babesia species in questing ticks: A systematic review and meta-analysis based on published literature. Pathogens. 2021; 10: 230. doi: 10.3390/pathogens10020230</mixed-citation><mixed-citation xml:lang="en">Onyiche TE, Răileanu C, Fischer S, Silaghi C. Global distribution of Babesia species in questing ticks: A systematic review and meta-analysis based on published literature. Pathogens. 2021; 10: 230. doi: 10.3390/pathogens10020230</mixed-citation></citation-alternatives></ref><ref id="cit3"><label>3</label><citation-alternatives><mixed-citation xml:lang="ru">Hildebrandt A, Zintl A, Montero E, Hunfeld KP, Gray J. Human babesiosis in Europe. Pathogens. 2021; 10: 1165. doi: 10.3390/pathogens10091165</mixed-citation><mixed-citation xml:lang="en">Hildebrandt A, Zintl A, Montero E, Hunfeld KP, Gray J. Human babesiosis in Europe. Pathogens. 2021; 10: 1165. doi: 10.3390/pathogens10091165</mixed-citation></citation-alternatives></ref><ref id="cit4"><label>4</label><citation-alternatives><mixed-citation xml:lang="ru">Birkenheuer AJ, Buch J, Beall MJ, Braff J, Chandrashekar R. Global distribution of canine Babesia species identified by a commercial diagnostic laboratory. Vet Parasitol Reg Stud Rep. 2020; 22: 100471. doi: 10.1016/j.vprsr.2020.100471</mixed-citation><mixed-citation xml:lang="en">Birkenheuer AJ, Buch J, Beall MJ, Braff J, Chandrashekar R. Global distribution of canine Babesia species identified by a commercial diagnostic laboratory. Vet Parasitol Reg Stud Rep. 2020; 22: 100471. doi: 10.1016/j.vprsr.2020.100471</mixed-citation></citation-alternatives></ref><ref id="cit5"><label>5</label><citation-alternatives><mixed-citation xml:lang="ru">Penzhorn BL. Don’t let sleeping dogs lie: Unravelling the identity and taxonomy of Babesia canis, Babesia rossi and Babesia vogeli. Parasit Vectors. 2020; 13: 184. doi: 10.1186/s13071-020-04062-w</mixed-citation><mixed-citation xml:lang="en">Penzhorn  BL. Don’t let sleeping dogs lie: Unravelling the identity and taxonomy of Babesia canis, Babesia rossi and Babesia vogeli. Parasit Vectors. 2020; 13: 184. doi: 10.1186/s13071-020-04062-w</mixed-citation></citation-alternatives></ref><ref id="cit6"><label>6</label><citation-alternatives><mixed-citation xml:lang="ru">Всемирная организация по охране здоровья животных (МЭБ). URL: https://www.oie.int [date of access: 10.10.2024].</mixed-citation><mixed-citation xml:lang="en">World Organisation for Animal Health. URL: https://www.oie.int [date of access: 10.10.2024].</mixed-citation></citation-alternatives></ref><ref id="cit7"><label>7</label><citation-alternatives><mixed-citation xml:lang="ru">Eshoo MW, Crowder CD, Carolan HE, Rounds MA, Ecker DJ, Haag H, et al. Broad-range survey of tick-borne pathogens in Southern Germany reveals a high prevalence of Babesia microti and a diversity of other tick-borne pathogens. Vector Borne Zoonotic Dis. 2014; 14: 584. doi: 10.1089/vbz.2013.1498</mixed-citation><mixed-citation xml:lang="en">Eshoo MW, Crowder CD, Carolan HE, Rounds MA, Ecker DJ, Haag H, et al. Broad-range survey of tick-borne pathogens in Southern Germany reveals a high prevalence of Babesia microti and a diversity of other tick-borne pathogens. Vector Borne Zoonotic Dis. 2014; 14: 584. doi: 10.1089/vbz.2013.1498</mixed-citation></citation-alternatives></ref><ref id="cit8"><label>8</label><citation-alternatives><mixed-citation xml:lang="ru">Moniuszko-Malinowska A, Swiecicka I, Dunaj J, Zajkowska J, Czupryna P, Zambrowski G, et al. Infection with Babesia microti in humans with non-specific symptoms in North East Poland. Infect Dis (Lond). 2016; 48: 537-543. doi: 10.3109/23744235.2016.1164339</mixed-citation><mixed-citation xml:lang="en">Moniuszko-Malinowska A, Swiecicka I, Dunaj J, Zajkowska J, Czupryna P, Zambrowski G, et al. Infection with Babesia microti in humans with non-specific symptoms in North East Poland. Infect Dis (Lond). 2016; 48: 537-543. doi: 10.3109/23744235.2016.1164339</mixed-citation></citation-alternatives></ref><ref id="cit9"><label>9</label><citation-alternatives><mixed-citation xml:lang="ru">Beattie JF, Michelson ML, Holman PJ. Acute babesiosis caused by Babesia divergens in a resident of Kentucky. N Engl J Med. 2002; 3(47): 697-698. doi: 10.1056/NEJM200208293470921</mixed-citation><mixed-citation xml:lang="en">Beattie  JF, Michelson  ML, Holman  PJ. Acute babesiosis caused by  Babesia divergens in  a  resident of  Kentucky. N Engl J Med. 2002; 3(47): 697-698. doi: 10.1056/NEJM200208293470921</mixed-citation></citation-alternatives></ref><ref id="cit10"><label>10</label><citation-alternatives><mixed-citation xml:lang="ru">Hong SH, Kim SY, Song BG, Rho JR, Cho CR, Kim CN, et al. Detection and characterization of an emerging type of Babesia sp. similar to Babesia motasi for the first case of human babesiosis and ticks in Korea. Emerg Microbes Infect. 2019; 8: 869-878. doi: 10 .1080/22221751.2019.1622997</mixed-citation><mixed-citation xml:lang="en">Hong SH, Kim SY, Song BG, Rho JR, Cho CR, Kim CN, et al. Detection and characterization of an emerging type of Babesia sp. similar to Babesia motasi for  the  first case of  human babesiosis and ticks in Korea. Emerg Microbes Infect. 2019; 8: 869-878. doi: 10 .1080/22221751.2019.1622997</mixed-citation></citation-alternatives></ref><ref id="cit11"><label>11</label><citation-alternatives><mixed-citation xml:lang="ru">Wilhelmsson P, Lövmar M, Krogfelt KA, Nielsen HV, Forsberg P, Lindgren PE. Clinical/serological outcome in humans bitten by Babesia species positive Ixodes ricinus ticks in Sweden and on the Åland Islands. Ticks Tick Borne Dis. 2020; 11: 101455. doi: 10.1016/j.ttbdis.2020.101455</mixed-citation><mixed-citation xml:lang="en">Wilhelmsson  P, Lövmar  M, Krogfelt  KA, Nielsen  HV, Forsberg P, Lindgren PE. Clinical/serological outcome in humans bitten by Babesia species positive Ixodes ricinus ticks in Sweden and on the Åland Islands. Ticks Tick Borne Dis. 2020; 11: 101455. doi: 10.1016/j.ttbdis.2020.101455</mixed-citation></citation-alternatives></ref><ref id="cit12"><label>12</label><citation-alternatives><mixed-citation xml:lang="ru">Doderer-Lang C, Filisetti D, Badin J, Delale C, Clavier V, Brunet J, et al. Babesia crassa-like human infection indicating need for adapted PCR diagnosis of babesiosis, France. Emerg Infect Dis. 2022; (2): 449-452. doi: 10.3201/eid2802.211596</mixed-citation><mixed-citation xml:lang="en">Doderer-Lang  C, Filisetti  D, Badin  J, Delale  C, Clavier V, Brunet J, et al. Babesia crassa-like human infection indicating need for adapted PCR diagnosis of babesiosis, France. Emerg Infect Dis. 2022; (2): 449-452. doi: 10.3201/eid2802.211596</mixed-citation></citation-alternatives></ref><ref id="cit13"><label>13</label><citation-alternatives><mixed-citation xml:lang="ru">Kumar A, O’Bryan J, Krause PJ. The global emergence of human babesiosis. Pathogens. 2021; 10: 1447. doi: 10.3390/pathogens10111447</mixed-citation><mixed-citation xml:lang="en">Kumar A, O’Bryan J, Krause PJ. The global emergence of human babesiosis. Pathogens. 2021; 10: 1447. doi:  10.3390/pathogens10111447</mixed-citation></citation-alternatives></ref><ref id="cit14"><label>14</label><citation-alternatives><mixed-citation xml:lang="ru">Рар В.А., Марченко В.А., Ефремова Е.А., Сунцова О.В., Лисак О.В., Тикунов А.Ю., и др. Идентификация и генетическая характеризация этиологического агента пироплазмидоза лошадей на территории Западной и Восточной Сибири. Вавиловский журнал генетики и селекции. 2018; 22(2): 224-229. doi: 10.18699/VJ18.351</mixed-citation><mixed-citation xml:lang="en">Rar VA, Marchenko VA, Efremova EA, Suntsova OV, Lisak OV, Tikunov AYu, et  al. Identification of  the  etiological agent of  equine piroplasmosis in Western and Eastern Siberia. Vavilov Journal of Genetics and Breeding. 2018; 22(2): 224-229. (In  Russ.). doi:  10.18699/VJ18.351</mixed-citation></citation-alternatives></ref><ref id="cit15"><label>15</label><citation-alternatives><mixed-citation xml:lang="ru">Рар В.А., Епихина Т.И., Ефремова Е.А., Марченко В.А., Сунцова О.В., Лисак О.В., и др. Молекулярно-генетический анализ возбудителей анаплазмозов сельскохозяйственных животных на территории Западной и Восточной Сибири. Acta biomedica scientifica. 2015; 105(5): 83-87.</mixed-citation><mixed-citation xml:lang="en">Rar VA, Yepikhina TI, Yefremova EA, MarchenkoVA, SuntsovaOV, LisakOV, et al. Molecular genetic analysis ofinfection agents offarm animals anaplasmosis on the territory of Western and Eastern Siberia. Acta biomedica scientifica. 2015; 105(5): 83-87. (In Russ.).</mixed-citation></citation-alternatives></ref><ref id="cit16"><label>16</label><citation-alternatives><mixed-citation xml:lang="ru">Standard Nucleotide BLAST. URL: http://www.ncbi.nlm.nih.gov/BLAST [date of access: 10.10.2024].</mixed-citation><mixed-citation xml:lang="en">Standard Nucleotide BLAST. URL: http://www.ncbi.nlm.nih.gov/BLAST [date of access: 10.10.2024].</mixed-citation></citation-alternatives></ref><ref id="cit17"><label>17</label><citation-alternatives><mixed-citation xml:lang="ru">MEGA 7.0. URL: http://www.megasoftware.net/manual.html [date of access: 10.10.2024].</mixed-citation><mixed-citation xml:lang="en">MEGA 7.0. URL:  http://www.megasoftware.net/manual.html [date of access: 10.10.2024].</mixed-citation></citation-alternatives></ref><ref id="cit18"><label>18</label><citation-alternatives><mixed-citation xml:lang="ru">Rar VA, Epikhina TI, Tikunova NV, Suntsova OV, Kozlova IV, Lisak OV, et al. Genetic variabilis of Babesia parasites in Haemaphysalis spp. and Ixodes persulcatus in the Baikal region and Far East of Russia. Infection, Genetics and Evolution. 2014; 28: 270-275. doi: 10.1016/j.meegid.2014.10.010</mixed-citation><mixed-citation xml:lang="en">Rar VA, Epikhina TI, TikunovaNV, SuntsovaOV, Kozlova IV, Lisak OV, et al. Genetic variabilis of Babesia parasites in Haemaphysalis spp. and Ixodes persulcatus in the Baikal region and Far East of Russia. Infection, Genetics and Evolution. 2014; 28: 270-275. doi: 10.1016/j.meegid.2014.10.010</mixed-citation></citation-alternatives></ref><ref id="cit19"><label>19</label><citation-alternatives><mixed-citation xml:lang="ru">Schnittger L, Yin H, Gubbels MJ, Beyer D, Niemann S, Jongejan F, et al. Phylogeny of sheep and goat Theileria and Babesia parasites. Parasitol Res. 2003; 91: 398-406. doi: 10.1007/s00436-003-0979-2</mixed-citation><mixed-citation xml:lang="en">Schnittger  L, Yin  H, Gubbels  MJ, Beyer  D, Niemann  S, Jongejan F, et al. Phylogeny of sheep and goat Theileria and Babesia parasites. Parasitol Res. 2003; 91: 398-406. doi:  10.1007/s00436-003-0979-2</mixed-citation></citation-alternatives></ref><ref id="cit20"><label>20</label><citation-alternatives><mixed-citation xml:lang="ru">Gou H, Guan G, Ma M, Liu A, Liu Z, Ren Q, et al. Phylogenetic analysis based on 28S rRNA of Babesia spp. In ruminants in China. Exp Appl Acarol. 2013; 59: 463-472. doi: 10.3347/kjp.2013.51.5.511</mixed-citation><mixed-citation xml:lang="en">Gou  H, Guan  G, Ma  M, Liu  A, Liu  Z, Ren  Q, et  al. Phylogenetic analysis based on  28S rRNA of Babesia  spp. In  ruminants in China. Exp Appl Acarol. 2013; 59: 463-472. doi: 10.3347/kjp.2013.51.5.511</mixed-citation></citation-alternatives></ref><ref id="cit21"><label>21</label><citation-alternatives><mixed-citation xml:lang="ru">Jia N, Zheng YC, Jiang JF, Jiang RR, Jiang BG, Wei R, et al. Human babesiosis caused by a Babesia crassa-like pathogen: A case series. Clin Infect Dis. 2018; 67: 1110-1119. doi: 10.1093/cid/ciy212</mixed-citation><mixed-citation xml:lang="en">Jia N, Zheng YC, Jiang JF, Jiang RR, Jiang BG, Wei R, et al. Human babesiosis caused by a Babesia crassa-like pathogen: A case series. Clin Infect Dis. 2018; 67: 1110-1119. doi: 10.1093/cid/ciy212</mixed-citation></citation-alternatives></ref><ref id="cit22"><label>22</label><citation-alternatives><mixed-citation xml:lang="ru">Сунцова О.В., Рар В.А., Лисак О.В., Мельцов И.В., Дорощенко Е.К., Савинова Ю.С., и др. Эпизоотическая ситуация в отношении гемопаразитарных заболеваний сельскохозяйственных животных в Иркутской области. Siberian Journal of Life Sciences and Agriculture. 2023; 15(4): 210-235. doi: 10.12731/2658-6649-2023-15-4-210-235</mixed-citation><mixed-citation xml:lang="en">Suntsova OV, Rar VA, Lisak OV, Meltsov IV, Doroshchenko EK, Savinova YuS, et al. Epizootic situation of hemoparasitic diseases of farm animals in Irkutsk region. Siberian Journal of Life Sciences and Agriculture. 2023; 15(4): 210-235. (In Russ.). doi: 10.12731/2658-6649-2023-15-4-210-235</mixed-citation></citation-alternatives></ref><ref id="cit23"><label>23</label><citation-alternatives><mixed-citation xml:lang="ru">Tirosh-Levy S, Gottlieb Y, Fry LM, Knowles DP, Steinman A. Тwenty years of equine piroplasmosis research: Global distribution, molecular diagnosis, and phylogeny. Pathogens. 2020; 8(9-11): 926. doi: 10.3390/pathogens9110926</mixed-citation><mixed-citation xml:lang="en">Tirosh-Levy S, Gottlieb Y, Fry LM, Knowles DP, Steinman A. Тwenty years of equine piroplasmosis research: Global distribution, molecular diagnosis, and phylogeny. Pathogens. 2020; 8(9-11): 926. doi: 10.3390/pathogens9110926</mixed-citation></citation-alternatives></ref><ref id="cit24"><label>24</label><citation-alternatives><mixed-citation xml:lang="ru">Rar V, Marchenko V, Suntsova O, Epikhina T, Tikunov A, Meltsov I, et al. The first study of the prevalence and genetic diversity of Theileria equi and Babesia caballi in horses in Russia. Parasitol Res.2024; 123: 279. doi: 10.1007/s00436-024-08300-3</mixed-citation><mixed-citation xml:lang="en">Rar V, Marchenko V, Suntsova O, Epikhina T, Tikunov A, Meltsov I, et al. The first study of the prevalence and genetic diversity of Theileria equi and Babesia caballi in horses in Russia. Parasitol Res.2024; 123: 279. doi: 10.1007/s00436-024-08300-3</mixed-citation></citation-alternatives></ref><ref id="cit25"><label>25</label><citation-alternatives><mixed-citation xml:lang="ru">Bajer A, Dwużnik-Szarek D. The specificity of Babesia tick vector interactions: Recent advances and pitfalls in molecular and field studies. Parasit Vectors. 2021; 14-1: 507. doi: 10.1186/s13071-021-05019-3</mixed-citation><mixed-citation xml:lang="en">Bajer A, Dwużnik-Szarek D. The specificity of Babesia tick vector interactions: Recent advances and  pitfalls in  molecular and  field studies. Parasit Vectors. 2021; 14-1: 507. doi:  10.1186/s13071-021-05019-3</mixed-citation></citation-alternatives></ref></ref-list><fn-group><fn fn-type="conflict"><p>The authors declare that there are no conflicts of interest present.</p></fn></fn-group></back></article>
