actabiomedicaActa Biomedica ScientificaActa Biomedica Scientifica2541-94202587-9596Scientific Centre for Family Health and Human Reproduction Problems10.29413/ABS.2024-9.4.2actabiomedica-4939Research ArticleАКУШЕРСТВО И ГИНЕКОЛОГИЯOBSTETRICS AND GYNAECOLOGYИндексы биоразнообразия микробиоты кишечника как маркеры гиперандрогенемии у женщин репродуктивного возрастаGut microbiota biodiversity indices as markers of hyperandrogenemia in women of reproductive agehttps://orcid.org/0000-0002-1078-3657ИгумновИ. А.IgumnovI. A.

664003, г. Иркутск, ул. Тимирязева, 16

Ilia A. Igumnov – Junior Research Officer at the Laboratory of Gynecological Endocrinology

Timirayzeva str. 16, Irkutsk 664003

lya.igumnov.86@bk.ruhttps://orcid.org/0000-0001-9720-068XБельковаН. Л.BelkovaN. L.

664003, г. Иркутск, ул. Тимирязева, 16

Natalia L. Belkova – Cand. Sc. (Biol.), Docent, Leading Research Officer at the Laboratory of Microbiome and Microecology

Timirayzeva str. 16, Irkutsk 664003

nlbelkova@gmail.comhttps://orcid.org/0000-0002-3407-9365АталянА. В.AtalyanA. V.

664003, г. Иркутск, ул. Тимирязева, 16

Alina V. Atalyan – Cand. Sc. (Biol.), Senior Research Officer at the Laboratory of Socially Significant Problems of Reproductology

Timirayzeva str. 16, Irkutsk 664003

alinaa@mail.ruhttps://orcid.org/0000-0003-0979-8816КлименкоЕ. С.KlimenkoE. S.

664003, г. Иркутск, ул. Тимирязева, 16

Elizaveta S. Klimenko – Junior Research Officer at the Functional Group of Genomic Research and Bioinformatics Analysis

Timirayzeva str. 16, Irkutsk 664003

klimenko.elizabet@gmail.comhttps://orcid.org/0000-0003-3588-6545ШолоховЛ. Ф.SholokhovL. F.

664003, г. Иркутск, ул. Тимирязева, 16

Leonid F. Sholokhov – Dr. Sc. (Med.), Professor, Head of the Laboratory of Physiology and Pathology of Endocrine System

Timirayzeva str. 16, Irkutsk 664003

lfshol@mail.ruhttps://orcid.org/0000-0003-4904-3709БеленькаяЛ. В.BelenkaiaL. V.

664003, г. Иркутск, ул. Тимирязева, 16

Liliia V. Belenkaia – Cand. Sc. (Med.), Senior Research Officer at the Laboratory of Physiology and Pathology of Endocrine System

Timirayzeva str. 16, Irkutsk 664003

drblv@mail.ruСамаринаЯ. М.SamarinaYa. M.

664003, г. Иркутск, ул. Тимирязева, 16

Yana M. Samarina– Laboratory Assistant at the Laboratory of Gynecological Endocrinology

Timirayzeva str. 16, Irkutsk 664003

samarina-yana2002@mail.ruhttps://orcid.org/0000-0002-8862-5771ДанусевичИ. Н.DanusevichI. N.

664003, г. Иркутск, ул. Тимирязева, 16

Irina N. Danusevich – Dr. Sc. (Med.), Leading Research Officer at the Laboratory of Gynecological Endocrinology

Timirayzeva str. 16, Irkutsk 664003

danusevich@sbamsr.irk.ruhttps://orcid.org/0000-0002-7662-8529ЛазареваЛ. М.LazarevaL. M.

664003, г. Иркутск, ул. Тимирязева, 16

Lyudmila M. Lazareva – Cand. Sc. (Med.), Research Officer at the Laboratory of Gynecological Endocrinology

Timirayzeva str. 16, Irkutsk 664003

lirken@mail.ruhttps://orcid.org/0000-0002-5747-7315НаделяеваЯ. Г.NadeliaevaIa. G.

664003, г. Иркутск, ул. Тимирязева, 16

Iana G. Nadeliaeva – Cand. Sc. (Med.), Research Officer at the Laboratory of Gynecological Endocrinology

Timirayzeva str. 16, Irkutsk 664003

ianadoc@mail.ruhttps://orcid.org/0000-0002-6271-7803СутуринаЛ. В.SuturinaL. V.

664003, г. Иркутск, ул. Тимирязева, 16

Larisa V. Suturina – Dr. Sc. (Med.), Professor, Chief Research Officer, Head of the Department of Reproductive Health

Timirayzeva str. 16, Irkutsk 664003

lsuturina@mail.ruФГБНУ «Научный центр проблем здоровья семьи и репродукции человека»Scientific Centre for Family Health and Human Reproduction Problems202425092024941218Copyright © Игумнов И.А., Белькова Н.Л., Аталян А.В., Клименко Е.С., Шолохов Л.Ф., Беленькая Л.В., Самарина Я.М., Данусевич И.Н., Лазарева Л.М., Наделяева Я.Г., Сутурина Л.В., 20242024Игумнов И.А., Белькова Н.Л., Аталян А.В., Клименко Е.С., Шолохов Л.Ф., Беленькая Л.В., Самарина Я.М., Данусевич И.Н., Лазарева Л.М., Наделяева Я.Г., Сутурина Л.В.Igumnov I.A., Belkova N.L., Atalyan A.V., Klimenko E.S., Sholokhov L.F., Belenkaia L.V., Samarina Y.M., Danusevich I.N., Lazareva L.M., Nadeliaeva I.G., Suturina L.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.actabiomedica.ru/jour/article/view/4939Введение

Введение. Ранее было показано, что «классические» фенотипы синдрома поликистозных яичников (СПЯ) связаны со значительным снижением альфа-разнообразия кишечной микробиоты по сравнению с показателями здоровых женщин.

Цель данного исследования. Установить пороговые значения для индексов альфа-разнообразия, значимых при синдроме поликистозных яичников с гиперандрогенией.

Материал и методы

Материал и методы. Врукописи представлены результаты, полученные в рамках «Исследования эпидемиологии и фенотипа СПЯ в Восточной Сибири», проведённого в Восточной Сибири (Россия) с 2016 по 2019 г. Все участники (175 женщин репродуктивного возраста: 26 женщин с СПЯ (согласно критериям, принятым в Роттердаме в 2003 г.) и гиперандрогенемией (повышенным уровнем общего тестостерона (Т), и/или индексом свободных андрогенов (ИСА), и/или уровнем дегидроэпиандростерон-сульфата (ДГЭА-С)) и 149 женщин без гиперандрогенемии) были рекрутированы во время ежегодного медосмотра по месту работы. Методы включали анкетирование, антропометрию и оценку по модифицированной шкале Ферримана – Галлвея, гинекологический осмотр, ультразвуковое исследование органов малого таза и анализы сыворотки крови на Т, ДГЭА-С, глобулин, связывающий половые гормоны, ИСА, пролактин, тиреотропный гормон и 17-OH-прогестерон. Пять индексов альфа-разнообразия микробиоты кишечника (ASV (amplicon sequencing variant), индекс Шеннона, индекс Симпсона, индекс Чао, и ACE (Abundance-based Coverage index)) оценивали с использованием метасеквенирования ампликонов. Статистический анализ включал ROC-анализ для разработки пороговых значений для индексов, ассоциированных с гиперандрогенией у женщин репродуктивного возраста с СПКЯ.

Результаты

Результаты. По результатам ROC-анализа наибольшая чувствительность при умеренной специфичности, с высокой площадью под кривой была установлена для индексов Шеннона и Симпсона с пороговыми значениями, классифицирующими женщин с гиперандрогенемией или без неё: 5,84 и 0,97 соответственно.

Выводы

Выводы. Разработанные критерии оценки альфа-разнообразия с использованиемпороговыхзначенийдлянаиболеезначимыхиндексовмогутбыть полезны для мониторинга результатов различных терапевтических вмешательств (пребиотики, пробиотики и т. д.) при гиперандрогенных фенотипах СПКЯ.

Introduction

Introduction. Previously, it was shown that the “classic” phenotypes of polycystic ovarian syndrome (PCOS) are associated with significant decrease in gut microbiota alpha diversity as compared with healthy women.

The aim of the study

The aim of the study. To establish cut-off points for alpha diversity indices, significant in polycystic ovarian syndrome with hyperandrogenism.

Material and methods

Material and methods. The manuscript presents a sub-study of Eastern Siberia PCOS Epidemiology and Phenotype Study, conducted in Eastern Siberia (Russia) from 2016 to 2019. All participants (175 women of reproductive age: 26 women with PCOS (according to Rotterdam criteria (2003)) and hyperandrogenemia (increased levels of total testosterone (TT) and/or free androgenindex(FAI), and/ordehydroepiandrosterone sulphate (DHEAS)), 149 – without hyperandrogenemia) were recruited during the annual employment medical assessment. Methods included a questionnaire survey, anthropometry and modified Ferriman – Gallwey score, gynecological examination, pelvic ultrasound, and blood serum tests for TT, DHEAS, sex hormone-binding globulin, FAI, prolactin, thyroid-stimulating hormone, and 17-hydroxyprogesterone. Five indices of alpha diversity (amplicon sequencing variant, Shannon index, Simpson index, Chao index, and abundance-based coverage Index) were estimated for the gut microbiota using amplicon metasequencing. Statistical analysis included ROC-analysis for development of cut-off points for the indices, associated with hyperandrogenism in women of reproductive age with PCOS.

Results

Results. According to results of ROC-analysis, the greatest sensitivity with moderate specificity, with a high area under the curve was established for the Shannon and Simpson indices with cut-off points classifying women with or without hyperandrogenemia – 5.84 and 0.97, respectively.

Conclusions

Conclusions. The developed criteria for assessing alpha diversity using cut-off points for the most significant indices can be useful for monitoring the results of different therapeutic interventions (prebiotics, probiotics, etc.) in hyperandrogenic phenotypes of PCOS.

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The authors declare that there are no conflicts of interest present.