actabiomedicaActa Biomedica ScientificaActa Biomedica Scientifica2541-94202587-9596Scientific Centre for Family Health and Human Reproduction Problems10.29413/ABS.2024-9.2.11actabiomedica-4733Research ArticleМОРФОЛОГИЯ, ФИЗИОЛОГИЯ И ПАТОФИЗИОЛОГИЯMORPHOLOGY, PHYSIOLOGY AND PATHOPHYSIOLOGYСреднетяжёлое и бессимптомное течение COVID-19 у женщин в менопаузе: биомаркеры системы антиоксидантной защитыMenopausal women with moderate and asymptomatic COVID-19: antioxidant defense system biomarkershttps://orcid.org/0000-0002-6512-1335СемёноваН. В.SemenovaN. V.

Семёнова Наталья Викторовна – доктор биологических наук, ведущий научный сотрудник лаборатории патофизиологии.

664003, Иркутск, ул. Тимирязева, 16

Natalya V. Semenova – Dr. Sc. (Biol.), Leading Research Officer at the Laboratory of Pathophysiology.

Timiryazeva str. 16, Irkutsk 664003

natkor_84@mail.ruhttps://orcid.org/0000-0001-7954-9570ВырупаеваЕ. В.VyrupaevaE. V.

Вырупаева Екатерина Викторовна – аспирант.

664003, Иркутск, ул. Тимирязева, 16

Ekaterina V. Vyrupaeva – Postgraduate.

Timiryazeva str. 16, Irkutsk 664003

goliafm@yandex.ruhttps://orcid.org/0000-0003-2124-6328КолесниковС. И.KolesnikovS. I.

Колесников Сергей Иванович – доктор медицинских наук, академик РАН, главный научный сотрудник.

664003, Иркутск, ул. Тимирязева, 16

Sergey I. Kolesnikov – Dr. Sc. (Med.), Member of RAS, Chief Scientific Officer.

Timiryazeva str. 16, Irkutsk 664003

sikolesnikov2012@gmail.comhttps://orcid.org/0000-0003-3255-2013ДаренскаяМ. А.DarenskayaM. A.

Даренская Марина Александровна – доктор биологических наук, ведущий научный сотрудник лаборатории патофизиологии.

664003, Иркутск, ул. Тимирязева, 16

Marina A. Darenskaya – Dr. Sc. (Biol.), Leading Research Officer at the Laboratory of Pathophysiology.

Timiryazeva str. 16, Irkutsk 664003

mops_my@front.ruhttps://orcid.org/0009-0001-1207-3309НовиковаЕ. А.NovikovaE. A.

Новикова Елизавета Анатольевна – лаборант-исследователь лаборатории биомедицинской микроэкологии.

664003, Иркутск, ул. Тимирязева, 16

Elizaveta A. Novikova – Clinical Research Assistant at the Laboratory of Biomedical Microecology.

Timiryazeva str. 16, Irkutsk 664003

yelizaveta_novikova_2001@bk.ruhttps://orcid.org/0000-0002-7965-8061ПетроваА. Г.PetrovaA. G.

Петрова Алла Германовна – доктор медицинских наук, профессор, заведующая лабораторией инфектологии и иммунопрофилактики в педиатрии.

664003, Иркутск, ул. Тимирязева, 16

Alla G. Petrova – Dr. Sc. (Med.), Professor, Head of the Laboratory of Infectology and Immunoprophylaxis in Pediatrics.

Timiryazeva str. 16, Irkutsk 664003

rudial75@gmail.comhttps://orcid.org/0000-0003-2169-0242ОрловаЕ. А.OrlovaE. A.

Орлова Елизавета Андреевна – младший научный сотрудник Института эпидемиологии и микробиологии.

664003, Иркутск, ул. Тимирязева, 16

Elizaveta A. Orlova – Junior Research Officer at the Institute of Epidemiology and Microbiology.

Timiryazeva str. 16, Irkutsk 664003

elizaveta.a.orlova@gmail.comhttps://orcid.org/0000-0003-3354-2992КолесниковаЛ. И.KolesnikovaL. I.

Колесникова Любовь Ильинична – доктор медицинских наук, профессор, академик РАН, научный руководитель.

664003, Иркутск, ул. Тимирязева, 16

Lyubov I. Kolesnikova – Dr. Sc. (Med.), Professor, Member of RAS, Scientific Advisor.

Timiryazeva str. 16, Irkutsk 664003

iphr@sbamsr.irk.ruФГБНУ «Научный центр проблем здоровья семьи и репродукции человека»Scientific Centre for Family Health and Human Reproduction Problems20240106202492112119Copyright © Семёнова Н.В., Вырупаева Е.В., Колесников С.И., Даренская М.А., Новикова Е.А., Петрова А.Г., Орлова Е.А., Колесникова Л.И., 20242024Семёнова Н.В., Вырупаева Е.В., Колесников С.И., Даренская М.А., Новикова Е.А., Петрова А.Г., Орлова Е.А., Колесникова Л.И.Semenova N.V., Vyrupaeva E.V., Kolesnikov S.I., Darenskaya M.A., Novikova E.A., Petrova A.G., Orlova E.A., Kolesnikova L.I.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.actabiomedica.ru/jour/article/view/4733Цель

Цель. Оценка общего антиоксидантного и глутатионового статуса, активности супероксиддисмутазы у женщин в менопаузе со среднетяжёлым и бессимптомным течением COVID-19.

Материалы и методы

Материалы и методы. Исследование включало 92 женщины в возрасте от 45 до 69 лет, которые были разделены на группы: женщины без COVID-19, не вакцинированные, без антител к SARS-CoV-2 (IgG) – контроль; женщины со среднетяжёлой формой заболевания COVID-19 – основная группа; женщины с IgG к SARS-CoV-2 в крови, но отрицающие какие-либо симптомы COVID-19 за последние 12 месяцев, – бессимптомное течение COVID-19.

Результаты

Результаты. У пациентов со среднетяжёлым течением COVID-19 выявлена более низкая активность глутатионпероксидазы (GPx), супероксиддисмутазы (СОД) и более высокая активность глутатионредуктазы (GR), концентрации глутатион-S-трансферазы pi (GSTpi) по сравнению с контролем. В группе с бессимптомным течением COVID-19 наблюдались статистически более низкие уровни окисленной формы глутатиона (GSSG), общего антиоксидантного статуса (TAS, total antioxidant status) и более высокие уровни восстановленного глутатиона (GSH), а также соотношение GSH/ GSSG по сравнению с контролем. У пациентов со среднетяжёлым течением COVID-19 выявлены статитсически значимо более низкие значения активности GPx, СОД и более высокие значения TAS, активности GR, GSTpi по сравнению с группой без клинических симптомов. ROC-анализ показал диагностическую значимость TAS (AUC (area under curve) – 0,714; p = 0,048), GSH (AUC = 0,714; p = 0,030), GSSG (AUC = 0,712; p = 0,031), GSH/GSSG (AUC = 0,837; p < 0,001) для группы с бессимптомным течением COVID-19 по сравнению с контрольной группой; TAS (AUC = 0,709; p = 0,020), СОД (AUC = 0,760; p < 0,001), GSH/GSSG (AUC = 0,658; p = 0,039), GPx (AUC = 0,774; p < 0,001), GSTpi (AUC = 0,864; p < 0,001) и GR (AUC = 0,871; p < 0,001) для группы со среднетяжёлым COVID-19 по сравнению с бессимптомным течением заболевания.

Заключение

Заключение. Активность системы антиоксидантной защиты у женщин в менопаузе зависит от течения COVID-19.

The aim

The aim. The research was conducted to assess the total antioxidant and glutathione status, superoxide dismutase activity in menopausal women with moderate and asymptomatic COVID-19.

Materials and methods

Materials and methods. Ninety two women 45 to 69 years old were divided into groups: women without COVID-19, not vaccinated, with no antibodies to SARS-CoV-2 (IgG) – control; women with moderate COVID-19 – main group; women with anti-SARS-CoV-2 IgG in blood but who deny any symptoms of COVID-19 in the last 12 months – asymptomatic COVID-19.

Results

Results. A lower glutathione peroxidase (GPx), superoxide dismutase (SOD) activities and a higher glutathione reductase (GR) activity, glutathione S-transferase pi (GSTpi) concentrations were detected in the patients with moderate COVID-19 as compared to control. There were statistically lower oxidized glutathione (GSSG) levels, total antioxidant status (TAS) and higher reduced glutathione (GSH) levels, as well as GSH/GSSG ratio in the group with asymptomatic COVID-19 as compared to control. Significantly a lower GPx, SOD activities and a higher TAS, GR activity, GSTpi concentrations were detected in the patients with symptomatic COVID-19 as compared to group without clinical symptoms. ROC analysis shows the diagnostic significance of TAS (AUC = 0.714; p = 0.048), GSH (AUC = 0.714; p = 0.030), GSSG (AUC = 0.712; p = 0.031), GSH/GSSG (AUC = 0.837; p < 0.001) for group with asymptomatic COVID-19 compared with controls; TAS (AUC = 0.709; p = 0.020), SOD (AUC = 0.760; p < 0.001), GSH/GSSG (AUC = 0.658; p = 0.039), GPx (AUC = 0.774; p < 0.001), GSTpi (AUC = 0.864; p < 0.001) and GR (AUC = 0.871; p < 0.001) for group with moderate COVID-19 compared asympotmatic COVID-19.

Conclusions

Conclusions. Antioxidant defense system activity in menopausal women depends on the COVID-19 course.

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The authors declare that there are no conflicts of interest present.