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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">actabiomedica</journal-id><journal-title-group><journal-title xml:lang="ru">Acta Biomedica Scientifica</journal-title><trans-title-group xml:lang="en"><trans-title>Acta Biomedica Scientifica</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">2541-9420</issn><issn pub-type="epub">2587-9596</issn><publisher><publisher-name>Scientific Centre for Family Health and Human Reproduction Problems</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.29413/ABS.2024-9.1.6</article-id><article-id custom-type="elpub" pub-id-type="custom">actabiomedica-4598</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>МИКРОБИОЛОГИЯ И ВИРУСОЛОГИЯ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>MICROBIOLOGY AND VIRUSOLOGY</subject></subj-group></article-categories><title-group><article-title>Биологические свойства и генетическая структура клинических изолятов комплекса видов Klebsiella pneumoniae</article-title><trans-title-group xml:lang="en"><trans-title>Biological properties and genetic structure of clinic isolates of Klebsiella pneumoniae species</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-9720-068X</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Белькова</surname><given-names>Н. Л.</given-names></name><name name-style="western" xml:lang="en"><surname>Belkova</surname><given-names>N. L.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Белькова Наталья Леонидовна – кандидат биологических наук, доцент, ведущий научный сотрудник, заведующая лабораторией микробиома и микроэкологии, </p><p>664003, г. Иркутск, ул. Тимирязева, 16</p></bio><bio xml:lang="en"><p>Natalia L. Belkova – Cand. Sc. (Biol.), Docent, Leading Research Officer at the Laboratory for Microbiome and Microecology, </p><p>Timiryazeva str. 16, Irkutsk 664003</p></bio><email xlink:type="simple">nlbelkova@gmail.com</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0003-0979-8816</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Клименко</surname><given-names>Е. С.</given-names></name><name name-style="western" xml:lang="en"><surname>Klimenko</surname><given-names>E. S.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Клименко Елизавета Станиславовна – младший научный сотрудник лаборатории микробиома и микроэкологии, </p><p>664003, г. Иркутск, ул. Тимирязева, 16</p></bio><bio xml:lang="en"><p>Elizaveta  S. Klimenko – Junior Research Officer at  the  Laboratory for  Microbiome and  Microecology,</p><p>Timiryazeva str. 16, Irkutsk 664003</p></bio><email xlink:type="simple">klimenko.elizabet@gmail.com</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-7656-342X</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Немченко</surname><given-names>У. М.</given-names></name><name name-style="western" xml:lang="en"><surname>Nemchenko</surname><given-names>U. M.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Немченко Ульяна Михайловна – кандидат биологических наук, научный сотрудник лаборатории микробиома и микроэкологии, </p><p>664003, г. Иркутск, ул. Тимирязева, 16</p></bio><bio xml:lang="en"><p>Ulyana  M. Nemchenko – Cand.  Sc. (Biol.), Research Officer at  the  Laboratory for  Microbiome and  Microecology, </p><p>Timiryazeva str. 16, Irkutsk 664003</p></bio><email xlink:type="simple">umnemch@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-6588-2591</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Григорова</surname><given-names>Е. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Grigorova</surname><given-names>E. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Григорова Екатерина Владимировна – кандидат биологических наук, научный сотрудник лаборатории микробиома и микроэкологии, </p><p>664003, г. Иркутск, ул. Тимирязева, 16</p></bio><bio xml:lang="en"><p>Ekaterina  V. Grigorova – Cand.  Sc. (Biol.), Research Officer at  the  Laboratory for  Microbiome and  Microecology, </p><p>Timiryazeva str. 16, Irkutsk 664003</p></bio><email xlink:type="simple">buxarowa.ekaterina@yandex.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-7717-906X</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Ситникова</surname><given-names>К. О.</given-names></name><name name-style="western" xml:lang="en"><surname>Sitnikova</surname><given-names>K. O.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Ситникова Ксения Олеговна – лаборант-исследователь лаборатории микробиома и микроэкологии, </p><p>664003, г. Иркутск, ул. Тимирязева, 16</p></bio><bio xml:lang="en"><p>Kseniya  O. Sitnikova – Clinical Research Assistant at  the  Laboratory for  Microbiome and  Microecology, </p><p>Timiryazeva str. 16, Irkutsk 664003</p></bio><email xlink:type="simple">butakovaksenia505@gmail.com</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0009-0000-8522-7359</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Зугеева</surname><given-names>Р. Е.</given-names></name><name name-style="western" xml:lang="en"><surname>Zugeeva</surname><given-names>R. E.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Зугеева Раиса Евгеньевна – лаборант лаборатории микробиома и микроэкологии,</p><p>664003, г. Иркутск, ул. Тимирязева, 16</p></bio><bio xml:lang="en"><p>Raisa E. Zugeeva – Clinical Research Assistant at the Laboratory for Microbiome and Microecology,</p><p>Timiryazeva str. 16, Irkutsk 664003</p></bio><email xlink:type="simple">raya.zugeeva@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0009-0004-3614-6631</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Смурова</surname><given-names>Н. Е.</given-names></name><name name-style="western" xml:lang="en"><surname>Smurova</surname><given-names>N. E.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Смурова Надежда Евгеньевна – лаборант-исследователь лаборатории микробиома и микроэкологии, </p><p>664003, г. Иркутск, ул. Тимирязева, 16</p></bio><bio xml:lang="en"><p>Nadezhda  E. Smurova – Clinical Research Assistant at  the  Laboratory for  Microbiome and  Microecology, </p><p>Timiryazeva str. 16, Irkutsk 664003</p></bio><email xlink:type="simple">nadinasmurova@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-5375-7785</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Чемезова</surname><given-names>Н. Н.</given-names></name><name name-style="western" xml:lang="en"><surname>Chemezova</surname><given-names>N. N.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Чемезова Наталья Николаевна – кандидат медицинских наук, врач-бактериолог, 664003, г. Иркутск, ул. Тимирязева, 16;</p><p>доцент кафедры эпидемиологии и микробиологии, 664049, г. Иркутск, Юбилейный, 100</p></bio><bio xml:lang="en"><p>Natalya N. Chemezova – Cand.  Sc. (Biol.), Bacteriologist, Timiryazeva str. 16, Irkutsk 664003;</p><p>Associate Professor at  the  Department of  Epidemiology and Microbiology, Yubileyny 100, Irkutsk 664049</p></bio><email xlink:type="simple">chemezova_nataly@mail.ru</email><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-9217-6876</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Савилов</surname><given-names>Е. Д.</given-names></name><name name-style="western" xml:lang="en"><surname>Savilov</surname><given-names>E. D.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Савилов Евгений Дмитриевич – доктор медицинских наук, профессор, главный научный сотрудник лаборатории эпидемиологически и социально-значимых инфекций, </p><p>664003, г. Иркутск, ул. Тимирязева, 16</p></bio><bio xml:lang="en"><p>Evgeny D. Savilov – Dr. Sc. (Med.), Professor, Chief Research Officer at the Laboratory of Epidemiologically and Socially Important Infections,</p><p>Timiryazeva str. 16, Irkutsk 664003</p></bio><email xlink:type="simple">savilov47@gmail.com</email><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>ФГБНУ «Научный центр проблем здоровья семьи и репродукции человека»</institution></aff><aff xml:lang="en"><institution>Scientific Centre for Family Health and Human Reproduction Problems</institution></aff></aff-alternatives><aff-alternatives id="aff-2"><aff xml:lang="ru"><institution>ФГБНУ «Научный центр проблем здоровья семьи и репродукции человека»;&#13;
Иркутская государственная медицинская академия последипломного образования – филиал ФГБОУ ДПО «Российская медицинская академия непрерывного профессионального образования» Минздрава России</institution></aff><aff xml:lang="en"><institution>Scientific Centre for Family Health and Human Reproduction Problems;&#13;
Irkutsk State Medical Academy of Postgraduate Education – Branch Campus of the Russian Medical Academy of Continuing Professional Education</institution></aff></aff-alternatives><pub-date pub-type="collection"><year>2024</year></pub-date><pub-date pub-type="epub"><day>25</day><month>03</month><year>2024</year></pub-date><volume>9</volume><issue>1</issue><fpage>53</fpage><lpage>63</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Белькова Н.Л., Клименко Е.С., Немченко У.М., Григорова Е.В., Ситникова К.О., Зугеева Р.Е., Смурова Н.Е., Чемезова Н.Н., Савилов Е.Д., 2024</copyright-statement><copyright-year>2024</copyright-year><copyright-holder xml:lang="ru">Белькова Н.Л., Клименко Е.С., Немченко У.М., Григорова Е.В., Ситникова К.О., Зугеева Р.Е., Смурова Н.Е., Чемезова Н.Н., Савилов Е.Д.</copyright-holder><copyright-holder xml:lang="en">Belkova N.L., Klimenko E.S., Nemchenko U.M., Grigorova E.V., Sitnikova K.O., Zugeeva R.E., Smurova N.E., Chemezova N.N., Savilov E.D.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://www.actabiomedica.ru/jour/article/view/4598">https://www.actabiomedica.ru/jour/article/view/4598</self-uri><abstract><p>Комплекс видов Klebsiella pneumoniae (Kp) представляет собой генетически и  экологически разнообразную группу бактерий, вызывающую широкий спектр инфекций у людей и животных.</p><sec><title>Цель исследования</title><p>Цель исследования. Биологическая характеристика и  генотипирование на  основе изучения разных локусов клинических изолятов Klebsiella pneumoniae.</p></sec><sec><title>Материалы и методы</title><p>Материалы и методы. Объектом исследования стали три клинических изолята Kp, выделенные из разных биотопов пациентов детского многопрофильного стационара регионального уровня. В работе использован комплекс бактериологических, молекулярно-генетических и  биоинформационных методов. Генотипирование изолятов проводили с использованием сервиса Института Пастера для штаммов видового комплекса K. pneumoniae.</p></sec><sec><title>Результаты</title><p>Результаты. Все штаммы были чувствительны к антимикробным препаратам групп карбапенемы (имипенем, меропенем) и тетрациклины (тигециклин) и демонстрировали высокую чувствительность к бактериофагу клебсиелл поливалентный. У  изолятов Kp ODKB-16 и  ODKB-81 отмечена антибиотикорезистентность к семи и восьми антимикробным препаратам соответственно.</p><p>Согласно результатам мультилокусного типирования, все штаммы отнесены к филогруппе Kp1, имели K2-тип и различались по сиквенс-типам, профилю scgMLST629 и KL-типу. Штамм Kp ODKB-16 был определён как ST-65, scgST-11107, KL2; ODKB-07 – как  ST-219, scgST-6401, KL125KL114; ODKB-81 – как  ST-86, scgST-2800, KL2KL30. Кластеры генов вирулентности AbST, CbST, YbST, SmST и  RmST были охарактеризованы только в  геноме изолята Kp ODKB-16, что  позволяет охарактеризовать его как  высоковирулентный с  множественной лекарственной устойчивостью (МЛУ). Дополнительно у всех штаммов выявлены гены, ответственные за синтез фимбриальных адгезинов 1-го и 3-го типов, а локусы ter-оперона – только у Kp ODKB-16. Анализ резистома показал, что все штаммы имели генотип 2b. Плазмиды были определены в геномах Kp ODKB-81 (IncI2) и ODKB-16 (IncFIA + IncFIB + IncHI1B).</p></sec><sec><title>Заключение</title><p>Заключение. Использована комплексная схема для геномной таксономии клинических изолятов, которая может способствовать унификации глобальных и  региональных особенностей возникновения и  микроэволюции бактериальных патогенов.</p></sec></abstract><trans-abstract xml:lang="en"><p>Klebsiella pneumoniae (Kp) species complex is a genetically and ecologically diverse group of bacteria that causes a wide range of infections in humans and animals.</p><sec><title>The aim</title><p>The aim. To carry out biological characterization and genotyping based on the study of different loci of Klebsiella pneumoniae clinical isolates.</p></sec><sec><title>Materials and  methods</title><p>Materials and  methods. The  object of  the study was  three Klebsiella pneumoniae clinical isolates from different biotopes of patients from a regional children’s multidisciplinary hospital. We used a complex of bacteriological, molecular genetic and bioinformatic methods. Genotyping of the isolates was carried out using the Pasteur Institute service for strains of the K. pneumoniae species complex.</p></sec><sec><title>Results</title><p>Results. All strains were sensitive to antimicrobial drugs from carbapenem (imipenem, meropenem) and  tetracycline groups (tigecycline), and  demonstrated high sensitivity to the Klebsiella polyvalent bacteriophage. The antibiotic resistance of the Kp ODKB-16 and ODKB-81 isolates to seven and eight antimicrobial drugs, respectively, was registered</p><p>Based on  the results of  multi-locus sequence typing, all  strains were assigned to  Kp1  phylogroup, K2  type and  differed in  sequence type, scgMLST629  profile and KL type. Kp ODKB-16 strain was identified as ST-65, scgST-11107, KL2; ODKB-07 strain – as ST-219, scgST-6401, KL125KL114; ODKB-81 strain – as ST-86, scgST-2800, KL2KL30. The virulence gene clusters AbST, CbST, YbST, SmST, and RmST have been characterized only in the genome of the Kp ODKB-16 isolate, allowing it to be characterized as highly virulent with multidrug resistance (MDR). Additionally, genes responsible for the synthesis of types 1 and 3 fimbrial adhesins were registered in all strains, and ter operon loci were identified only in Kp ODKB-16. Resistome analysis showed that all strains had 2b genotype. Plasmids were found in the genomes of Kp ODKB-81 (IncI2) and ODKB-16 (IncFIA + IncFIB + IncHI1B).</p></sec><sec><title>Conclusion</title><p>Conclusion. We used a comprehensive framework for genomic taxonomy of clinical isolates, which can contribute to the unification of global and regional peculiarities of the developing and microevolution of bacterial pathogens.</p></sec></trans-abstract><kwd-group xml:lang="ru"><kwd>комплекс видов Klebsiella pneumoniae</kwd><kwd>генотипирование</kwd><kwd>мультилокусный анализ</kwd><kwd>множественная антибиотикорезистентность</kwd><kwd>антимикробные препараты</kwd><kwd>вирулентность</kwd></kwd-group><kwd-group xml:lang="en"><kwd>Klebsiella pneumoniae species complex</kwd><kwd>genotyping</kwd><kwd>multilocus analysis</kwd><kwd>multiple antibiotic resistance</kwd><kwd>antimicrobial drugs</kwd><kwd>virulence</kwd></kwd-group><funding-group><funding-statement xml:lang="ru">Исследование выполнено в  рамках госзадания № 121022500179-0.</funding-statement></funding-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Wyres KL, Lam MMC, Holt KE. 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