actabiomedicaActa Biomedica ScientificaActa Biomedica Scientifica2541-94202587-9596Scientific Centre for Family Health and Human Reproduction Problems10.12737/article_5955e6b5aad2e3.30269730actabiomedica-369Research ArticleЭКСПЕРИМЕНТАЛЬНЫЕ ИССЛЕДОВАНИЯ В БИОЛОГИИ И МЕДИЦИНЕEXPERIMENTAL RESEARCHES IN BIOLOGY AND MEDICINEОЦЕНКА ВИРУСНЕЙТРАЛИЗУЮЩИХ СВОЙСТВ ДНК-АПТАМЕРОВ И ЭКСТРАКТОВ ЛЕКАРСТВЕННЫХ РАСТЕНИЙ В ОТНОШЕНИИ ВИРУСА КЛЕЩЕВОГО ЭНЦЕФАЛИТАASSESSMENT OF NEUTRALIZING PROPERTIES OF DNA-APTAMERS AND EXTRACTS OF MEDICINAL HERBS AGAINST THE TICK-BORNE ENCEPHALITIS VIRUSСоловаровИ. С.SolovarovI. S.keschass@mail.ruХаснатиновМ. А.KhasnatinovM. A.khasnatinov@yandex.ruДанчиноваГ. А.DanchinovaG. A.dan-chin@yandex.ruЛяпуновА. В.LiapunovA. V.liapunov.asp@mail.ruБолотоваН. А.BolotovaN. A.nataly2193@mail.ruМанзароваЭ. Л.ManzarovaE. L.manzarova89@yandex.ruКондратовИ. Г.KondratovI. G.igkondratov@rambler.ruБеликовС. И.BelikovS. I.sergeibelikov47@gmail.ruФГБНУ «Научный центр проблем здоровья семьи и репродукции человека»Scientific Centre for Family Health and Human Reproduction ProblemsФГБУН Лимнологический институт Сибирского отделения Российской академии наукLimnological Institute of Siberian Branch of the Russian Academy of Sciences201728022017218488Copyright © Соловаров И.С., Хаснатинов М.А., Данчинова Г.А., Ляпунов А.В., Болотова Н.А., Манзарова Э.Л., Кондратов И.Г., Беликов С.И., 20172017Соловаров И.С., Хаснатинов М.А., Данчинова Г.А., Ляпунов А.В., Болотова Н.А., Манзарова Э.Л., Кондратов И.Г., Беликов С.И.Solovarov I.S., Khasnatinov M.A., Danchinova G.A., Liapunov A.V., Bolotova N.A., Manzarova E.L., Kondratov I.G., Belikov S.I.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.actabiomedica.ru/jour/article/view/369

Была проведена работа по получению аптамеров к вирусу клещевого энцефалита, а также оценка их нейтрализующей активности. В качестве нейтрализующих агентов были использованы 2 аптамера и 2 субстрата из растений из Монголии. Кроме того, был поставлен эксперимент на возможности маскирования аптамерами вирусных частиц от нейтрализующих их антител. В результате один из экстрактов растений показал нейтрализующую активность по ингибированию инвазивности вируса клещевого энцефалита.

Tick borne encephalitis (TBE) is a dangerous neurological disease that is transmitted to humans through the bite of Ixodid ticks. The disease exhibits an estimated 16 000 cases recorded annually over 30 European and Asian countries. The agent of TBE is the tick borne encephalitis virus (TBEV), belonging to the family Flaviviridae, genus Flavivirus. In spite the significant impact of TBE on human health, there is a serious lack of specific treatment against this disease. The only specific drug available is the human anti-TBEV immunoglobulin from vaccinated blood donors. The drug is produced and used in Russia only, both to prevent and to cure the TBE. In this work, we evaluated the ability of TBEV-specific DNA-aptamers and extracts of traditional medicine plants to neutralize the TBEV Selection of aptamers was performed using SELEX approach. Extracts of the seeds of Momordica cochinchinensis and Terminalia chebula were produced by boiling the ground seeds in water, clarified by centrifugation and filtration steps and filter sterilized. The SELEX had produced two aptamers - My13 and My38. Neither of two was capable to neutralize TBEV in vitro. The ability to shield the antibody binding sites on the surface of TBEV virions was also absent. The extract of M. cochinchinensis exhibited no neutralizing activity as well. Surprisingly, the T. chebula extract completely neutralized the TBEV after 30 min of incubation at 37 °C. The possible explanations and further development of the project are discussed.

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The authors declare that there are no conflicts of interest present.