References

actabiomedica

Acta Biomedica Scientifica

2541-94202587-9596

Scientific Centre for Family Health and Human Reproduction Problems

10.12737/23419

actabiomedica-303

Research Article

ГЕНЕТИКА И ПРОТЕОМИКА

GENETICS AND PROTEOMICS

Полиморфный локус Q223R гена LEPR и ожирение

Q223R polymorphism of the LEPR and obesity

Иевлева

К. Д.

Ievleva

K. D.

asiy91@mail.ru

Рычкова

Л. В.

Rychkova

L. V.

iphr@sbamsr.irk.ru

Шенеман

Е. А.

Sheneman

E. A.

sheneman@bk.ru

Баирова

Т. А.

Bairova

T. A.

tbairova38@mail.ru

ФГБНУ «Научный центр проблем здоровья семьи и репродукции человека»Scientific Centre for Family Health and Human Reproduction Problems

2016

28092016

15170174

2016

Иевлева К.Д., Рычкова Л.В., Шенеман Е.А., Баирова Т.А.

Ievleva K.D., Rychkova L.V., Sheneman E.A., Bairova T.A.

Данная работа распространяется под лицензией Creative Commons Attribution 4.0.

This work is licensed under a Creative Commons Attribution 4.0 License.

https://www.actabiomedica.ru/jour/article/view/303

Цель: выявить ассоциацию полиморфизма LEPR Q223R с избыточной массой тела и ожирением у девочек подросткового возраста. Исследовано 123 девочки подросткового возраста: 58 человек (SDS ИМТ ± 1,0) - контрольная группа и 65 человек (SDS ИМТ > +1,0-2,0) - основная группа. Использовались антропометрические, молекулярногенетические и статистические методы. Частота G-аллели в контрольной группе составила 43,1 %, в основной группе - 40,0 %. Между группами не выявлены статистически значимые различия распространенности G-аллели (р = 0,862).

The problem of overweight and obesity is one of the most urgent health issues in the world. 13 % of girls and 21 % of boys aged 11 suffer from overweight in the Russian Federation. The main causes of pubertal obesity are endocrine pathology, lifestyle and genetic disorders including mutation and polymorphisms of different metabolic pathways. Leptin produced in adipose tissue participates in reproduction regulation, glucose homeostasis, bone formation, etc. These effects are provided by leptin receptors coding LEPR gene. Q223R (rs1137101) polymorphism is associated with an increased serum level of leptin and overweight. There is no exact information about association between this polymorphism and obesity of adolescent females. The objective was to reveal LEPR Q223R polymorphism association between overweight and obesity in adolescent females. 123 Caucasian adolescent females were involved in this study. All samples could be separated into two groups: the girls with normal weight (SDS BM1 ± 1.0; control group), girls with overweight and obesity (SDS BM1 > +1.0-2.0; studied group). Anthropometric measurements (weight, height, waist and hip circumference, body fat percentage) were taken, and genotyping was performed using polymerase chain reaction with electrophoresis detection. G-allele frequency was 43.1 % in control and 40 % in the clinical group. We found no significant differences of the prevalence of polymorphism Q223R between the studied groups (р = 0,862). Furthermore, there was no association between the carriage of AG and GG with weight, BM1, body fat percentage, waist and hip circumference in both groups (р > 0.05). We have not found any association between LEPR Q223R and overweight and obesity in adolescent females.

подростковый возрастизбыточная масса телаожирениеполиморфный локус Q223Rген LEPR

adolescent femalesoverweightobesityLEPR Q223R

References1

Аметов А.С. Избранные лекции по эндокринологии. - М., МИА, 2009. - 496 с

Ахмедова Р.М., Софронова Л.В. Ожирение и метаболический синдром в детском возрасте: современный взгляд на проблему // Вопросы диагностики в педиатрии. - 2012. - Т. 4, № 1. - C. 13-19

Ковалева Ю.В. Роль ожирения в развитии нарушений менструальной и репродуктивной функций // Российский вестник акушера-гинеколога. - 2014. -№ 2. - С. 43-51

Николаев И.В., Мулюкова Р.В., Каюмова Л.Р., Воробьева Е.В. Анализ взаимодействия аллелей генов липидного обмена при дислипидемии // Вавиловский журнал генетики и селекции. - 2014. - Т. 18, № 4/2. - С. 856-866

Щербакова М.Ю., Порядина Г.И. Современный взгляд на проблему ожирения у детей и подростков // Педиатрия. - 2012. - Т. 91, № 3. - С. 122-130

Allele frequency for polymorphic site: rs1137101. The allele frequency database. Available at: https://alfred.med.yale.edu/alfred/SiteTable1A_working.asp?siteuid=SI096978N.

Bender N., Allemann N., Marek D., Vollenweider P., Waeber G., Mooser V., Egger M., Bochud M. (2011). Association between LEPR and Obesity Association between variants of the Leptin Receptor Gene (LEPR) and overweight: a systematic review and an analysis of the CoLaus study. Plos One, 6 (10), e26157.

Boumaiza I., Omezzine A., Rejeb J., Rebhi L., Ouedrani A., Rejeb N.B., Nabli N., Abdelaziz A.B., Bouslama A. (2012). Relationship between Leptin G2548A and leptin receptor Q223R gene polymorphisms and obesity and metabolic syndrome risk in Tunisian volunteers. Genetic Testing and Molecular Biomarkers, 16 (7), 726-733.

Constantin A., Costache G., Sima A.V., Glavce C.S., Vladica M. (2010). Leptin G-2548A and leptin receptor Q223R gene polymorphisms are not associated with obesity in Romanian subjects. Biochem Biophys Res Commun, (391), 282-286.

Domi'nguez-Reyes T., Astudillo-Lôpez C.C., Salgado-Goytia L., Munoz-Valle J.F., Salgado-Bernabé1 A.B., Guzman-Guzman I.P., Castro-Alarcôn N., Moreno-Godi'nez M.E., Parra-Rojas I. (2015). Interaction of dietary fat intake with APOA2, APOA5 and LEPR polymorphisms and its relationship with obesity and dyslipidemia in young subjects. Lipids in Health and Disease. 14 (106), available at: http://creativecommons.org/licenses/by/4.0/

Fan S.H., Fan Y.H.S. (2014). Leptin and leptin receptor gene polymorphisms and their association with plasma leptin levels and obesity in a multi-ethnic Malaysian suburban population. Journal of Physiological Anthropology, (33), 15.

Garver W.S., Newman S.B., Gonzales-Pacheco D.M., Castillo J.J., Jelinek D., Heidenreich R.A., Orlando R.A. (2013). The genetics of childhood obesity and interaction with dietary macronutrients. Genes Nutr., (8), 271-287.

Gill1 R., Cheung Y.H., Shen Y., Lanzano P., Mirza N.M., Ten S., Maclaren N.K., Motaghedi R., Han J.C., Yanovski J.A. et al. (2014). Whole-exome sequencing identifies novel LEPR mutations in individuals with severe early onset obesity. Obesity (Silver Spring), 22 (2), 576-584.

LEPR gene (protein coding). Gene cards human gene database (2015). Available at: http://www.genecards.org/cgi-bin/carddisp.pl?gene=LEPR&keywords=Lepr.

Mutch D.M., Clement K. (2006). Unraveling the genetics of human obesity. PlosOne, 2 (12), e188.

Pyzzak B., Wiswiewska A., Kuckarska A., Wasik M., Demkov V. (2009). No association of LEPR Gln223Arg polymorphism with leptin, obesity or metabolic disturbance in children. Eur. J. Med. Res., 14 (4), 201-209.

Queiroz E.M., Candido A.P.C., Castro I.M., Bastos A.Q.A., Machado-Coelho G.L.L., Freitas R.N. (2015). IGF2, LEPR, POMC, PPARG, and PPARGC1 gene variants are associated with obesity-related risk phenotypes in Brazilian children and adolescents. Brazilian J. Med. Biol. Res., 48 (7), 595-602.

Qui L., Cho Y.A. (2008) Gene-environment interaction and obesity. Nutr. Rev., 66 (12), 684-694.

Riestra P., Garcia-Anguita A., Schoppen S., Lopez-Simon L., De Oya M., Garces C. (2010). Sex-specific association between leptin receptor polymorphisms and leptin levels and BMI in healthy adolescents. Acta Paediatr., (99), 1527-1530.

Sahin S., Rüstemollu A., Tekcan I.A., Tagliyurt T., Güven H., Yigit S. (2013). Investigation of associations between obesity and LEP G2548A and LEPR 668A/G polymorphisms in a Turkish population. Disease Markers, 35 (6), 673-677.

Yiannakouris N., Yannakoulia M., Melistas L., Chan J.L., Klimis-Zacas D., Mantzoros C.S. (2001). The Q223R polymorphism of the leptin receptor gene is significantly associated with obesity and predicts a small percentage of body weight and body composition variability. J. Clin. Endocrinol. Metab., (86), 4434-443.

The authors declare that there are no conflicts of interest present.