actabiomedicaActa Biomedica ScientificaActa Biomedica Scientifica2541-94202587-9596Scientific Centre for Family Health and Human Reproduction Problems10.29413/ABS.2021-6.4.24actabiomedica-2998Research ArticleЭКСПЕРИМЕНТАЛЬНЫЕ ИССЛЕДОВАНИЯEXPERIMENTAL RESEARCHESВлияние экзогенного мелатонина на антиоксидантную защиту печени и тонкого кишечника сирийского хомяка (Mesocricetus auratus)Effect of exogenous melatonin on the antioxidant defense system in the liver and small intestine of the Syrian hamster (Mesocricetus auratus)https://orcid.org/0000-0002-4740-2141АнтоноваЕ. П.AntonovaE. P.

 кандидат биологических наук, старший научный сотрудник лаборатории экологической физиологии животных

185910, г. Петрозаводск, ул. Пушкинская, 11, Россия

 Cand. Sc. (Biol.), Senior Research Officer at the Laboratory of Animal Ecophysiology

 Pushkinskaya str. 11, Petrozavodsk 185910, Russian Federation 

antonova88ep@mail.ruhttps://orcid.org/0000-0002-0016-7017ВолодинаА. Д.VolodinaA. D.

 ведущий биолог лаборатории экологической физиологии животных

185910, г. Петрозаводск, ул. Пушкинская, 11, Россия

 Leading Biologist at the Laboratory of Animal Ecophysiology 

Pushkinskaya str. 11, Petrozavodsk 185910, Russian Federation 

myshonog@rambler.ruhttps://orcid.org/0000-0002-7085-4154ИлюхаВ. А.IlyukhaV. A.

 доктор биологических наук, директор

185910, г. Петрозаводск, ул. Пушкинская, 11, Россия

 Dr. Sc. (Biol.), Director 

Pushkinskaya str. 11, Petrozavodsk 185910, Russian Federation 

ilyukha@bio.krc.karelia.ruИнститут биологии – обособленное подразделение ФГБУН Федеральный исследовательский центр «Карельский научный центр РАН»Institute of Biology of Karelian Research Centre of the Russian Academy of Sciences 20211210202164265272Copyright © Антонова Е.П., Володина А.Д., Илюха В.А., 20212021Антонова Е.П., Володина А.Д., Илюха В.А.Antonova E.P., Volodina A.D., Ilyukha V.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.actabiomedica.ru/jour/article/view/2998

Обоснование. По причине возрастающего светового загрязнения и освоения новых северных территорий поиск препаратов, повышающих адаптационные возможности организма, является перспективным.Цель исследования: изучить влияние экзогенного мелатонина на антиоксидантную защиту печени и тонкого кишечника самок сирийского хомяка (Mesocricetus auratus) в фотопериодических условиях Севера.Материалы и методы. Хомяки были разделены на две группы: контроль (LD: 12 ч свет/12 ч темнота; n = 12) и опыт (NL: постепенное уменьшение продолжительности световой фазы дня от 19:36 ч/4:24 ч до 12 ч/12 ч, наблюдаемое в г. Петрозаводске в период с 25.06.18 по 25.09.18; n = 24). В целях коррекции физиологического состояния хомякам группы NL добавляли в воду мелатонин в ночное время (100 мкг/животное) (NL + mel).Результаты. Содержание животных в NL-режиме привело к снижению содержания глутатиона и активностей супероксиддисмутазы (СОД) и каталазы на начальном этапе эксперимента и повышению уровня ТБК-реактивных продуктов (ТБК-РП) в начале и через месяц эксперимента в печени по сравнению с LD-режимом. Обнаружено, что в тонком кишечнике активность супероксиддисмутазы, а также уровни глутатиона (начальный и промежуточный этапы) и ТБК-РП (конец опыта) были значительно выше в группе NL, чем у хомяков группы LD. Концентрации ТБК-РП в печени и тонком кишечнике через 1 и 3 месяца были снижены в группе NL + mel по сравнению с группой NL.Заключение. Полученные результаты свидетельствуют о чувствительности антиоксидантной защиты в тканях печени и тонкого кишечника к фотопериоду и экзогенному мелатонину у сирийского хомяка. Показано, что экзогенный мелатонин способен снижать уровень ТБК-РП и повышать активность СОД и каталазы в световых условиях Севера (NL + mel).

Background. Due to the growing light pollution and the development of new territories, including northern ones, the search for drugs that increase the adaptive capacity of the organism is promising.The aim. We studied the effects of the exogenous melatonin (100 µg/day/animal) on antioxidant status of liver and small intestine in Syrian hamsters (Mesocricetus auratus) in the light conditions of North-West of Russia (Petrozavodsk, northern lighting – NL).Materials and methods. Female hamsters were exposed to a 12/12 light/dark cycle (LD; n = 12) or NL for 3 months. In NL light conditions hamsters were divided into two groups: NL-control (received placebo; n = 12) and NL-mel (received melatonin; n = 12). The study was conducted from the period of the summer solstice – June 25 (NL: 19.36/4.24) to September 25 (NL: 12/12) (autumn equinox).Results. Animals were kept in the NL conditions had decreased the levels of GSH and activities of antioxidant enzymes (superoxide dismutase (SOD) and catalase) at initial stage of experiment as well as increased TBA reactive substances (TBARS) level at the beginning and after a month of the experiment in the liver in comparison to control (LD). It was observed that in the small intestine the activities of SOD and the levels of GSH (initial and intermediate stages) and TBARS (end of the experiment) were significantly higher in NL in comparison to LD. Liver and small intestine TBARS concentrations after one and three months of the experiment were decreased in NL-mel in comparison to NL-control.Conclusion. The results of the study indicate the sensitivity of the antioxidant defense system in the tissues of the liver and small intestine of Syrian hamster to the photoperiod and exogenous melatonin. The present study revealed that exogenous melatonin was able to reduce the level of TBARS and increase the activity of SOD and CAT in the light conditions of North-West of Russia.

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The authors declare that there are no conflicts of interest present.