actabiomedicaActa Biomedica ScientificaActa Biomedica Scientifica2541-94202587-9596Scientific Centre for Family Health and Human Reproduction Problems10.12737/23404actabiomedica-290Research ArticleЭКСПЕРИМЕНТАЛЬНЫЕ ИССЛЕДОВАНИЯ В БИОЛОГИИ И МЕДИЦИНЕEXPERIMENTAL RESEARCHES IN BIOLOGY AND MEDICINEЭкспрессия молекулярных лимфатических маркеров в сосудистой оболочке глаза человекаExpression of molecular lymphatic markers in human choroidНоговицинаС. Р.NogovitsinaS. R.nogovitsina.niikel@gmail.comГБНУ «Научно-исследовательский клинической и экспериментальной лимфологии»Research Institute of Clinical and Experimental Lymphology20162809201615114116Copyright © Ноговицина С.Р., 20162016Ноговицина С.Р.Nogovitsina S.R.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.actabiomedica.ru/jour/article/view/290

Целью работы было выявление лимфатических путей оттока в сосудистой оболочке глаза человека. Проведено иммуногистохимическое исследование хориоидеи с использованием антител к специфическим лимфатическим и кровеносным эндотелиальным маркерам. Выявлены Prox-1+-, LYVE-1+-, и Podoplanin+- каналы и лакуны, ограниченные эндотелиоподобными клетками и фибробластами. Положительное окрашивание на маркеры лимфатических сосудов и отсутствие их окрашивания на маркер кровеносных сосудов CD34 дает основание считать выявленные структуры лимфатическими.

The purpose of this study was to research lymphatic outflow tract in the human choroid. Using a light microscopy and immunohistochemistry, structure of the choroid was investigated. We used antibodies against lymphatic endothelial-specific markers Prox-1, LYVE-1, Podoplanin, endothelial blood vessels marker CD34 and fibroblasts growth factor receptor FGFR. The choroid was found to contain lymphatic canals in choroidal stroma and a layer of choriocapillaris, and lymphatic lacunae in the suprachoroid lamina, limited endothelium-like cells, positively stained for markers of lymphatic vessels Prox-1, LYVE-1, Podoplanin, and fibroblasts and pigment cells. These endothelium-like cells were shown positive Prox-1, LYVE-1 and Podoplanin staining and negative CD34 staining; therefore we consider that these canals and lacunae are lymphatic structures.

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The authors declare that there are no conflicts of interest present.